Production of thermostable invertases by Aspergillus caespitosus under submerged or solid state fermentation using agroindustrial residues as carbon source

The production cost of β-glucosidase and endoglucanase could be reduced by using water hyacinth, an aquatic weed, as the sole carbon source and using cost-efficient fermentation strategies like solid-state fermentation (SSF). In the present study, the effect of different production conditions on the yield of β-glucosidase and endoglucanase by Rhizopus oryzae MTCC 9642 from water hyacinth was investigated systematically using response surface methodology. A Central composite experimental design was applied to optimize the impact of three variables, namely, substrate concentration, pH, and temperature, on enzyme production. The optimal level of each parameter for maximum enzyme production by the fungus was determined. Highest activity of endoglucanase of 495 U/mL was achieved at a substrate concentration of 1.23%, pH 7.29, and temperature 29.93°C whereas maximum β-glucosidase activity of 137.32 U/ml was achieved at a substrate concentration of 1.25%, pH 6.66, and temperature 32.09°C. There was a direct correlation between the levels of enzymatic activities and the substrate concentration of water hyacinth as carbon source.

Download Full-text

Production of β-glucosidase on solid-state fermentation by Lichtheimia ramosa in agroindustrial residues: Characterization and catalytic properties of the enzymatic extract

Electronic Journal of Biotechnology ◽

10.1016/j.ejbt.2015.05.007 ◽

2015 ◽

Vol 18 (4) ◽

pp. 314-319 ◽

Cited By ~ 37

Author(s):

Nayara Fernanda Lisboa Garcia ◽

Flávia Regina da Silva Santos ◽

Fabiano Avelino Gonçalves ◽

Marcelo Fossa da Paz ◽

Gustavo Graciano Fonseca ◽

...

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Catalytic Properties ◽

Enzymatic Extract ◽

Agroindustrial Residues ◽

Lichtheimia Ramosa

Download Full-text

Influence of Carbon, Nitrogen and Phosphorous Sources on Glucoamylase Production by Aspergillus awamori in Solid State Fermentation

Zeitschrift für Naturforschung C ◽

10.1515/znc-2003-9-1020 ◽

2003 ◽

Vol 58 (9-10) ◽

pp. 708-712 ◽

Cited By ~ 15

Author(s):

Telma Elita Bertolin ◽

Willibaldo Schmidell ◽

Alfredo E. Maiorano ◽

Janice Casara ◽

Jorge A. V. Costa

Keyword(s):

Solid State ◽

Carbon Source ◽

Solid State Fermentation ◽

Carbon Sources ◽

Aspergillus Awamori ◽

Main Carbon Source ◽

Nitrogen Phosphorus ◽

Additional Carbon Source ◽

Main Carbon ◽

Glucoamylase Production

AbstractIt was the objective of the present study to increase the production of glucoamylase by Aspergillus awamori through solid state fermentation, using wheat bran as the main carbon source and (NH4)2SO4, urea, KH2PO4, glucose, maltose and starch as additional nitrogen, phosphorus, and carbon sources. The production of glucoamylase is strongly influenced by N and C sources. A 100% increase was observed when the (NH4)2SO4 was replaced by urea, with C/N = 4.8, using maltose as the additional carbon source. C/P ratios in a range of 5.1 to 28.7 did not induce glucoamylase production under the studied conditions.

Download Full-text