scholarly journals Expression pattern of glycoconjugates in the Bidderian and ovarian follicles of the Brazilian toad Bufo ictericus analyzed by lectin histochemistry

2006 ◽  
Vol 66 (1a) ◽  
pp. 45-51 ◽  
Author(s):  
C. F. Farias ◽  
R. A. Azevedo ◽  
L. Brito-Gitirana
Keyword(s):  
Zona Pellucida ◽  
Ricinus Communis ◽  
Periodic Acid ◽  
Lectin Histochemistry ◽  
Sugar Residue ◽  
Follicular Cells ◽  
Periodic Acid Schiff ◽  
Ulex Europaeus ◽  
Alpha Beta ◽  
Toad Bufo

The Bidder's organ and ovary of the Brazilian toad Bufo ictericus were studied by light microscopy, using hematoxylin-eosin (HE) and periodic acid Schiff (PAS) staining. The expression and distribution of carbohydrate moieties was analyzed by lectin histochemistry, using 8 lectins with different carbohydrate specificities: Ulex europaeus (UEA I), Lens culinaris (LCA), Erythrina cristagalli (ECA), Arachis hypogaea (PNA), Ricinus communis (RCA I), Aleuria aurantia (AAA), Triticum vulgaris (WGA), and Glycine maximum (SBA). The results showed that the Bidderian zona pellucida presented alpha-mannose, alpha-L-fucose, beta-D-galactose, N-acetyl-D-glucosamine, and alpha/beta-N-acetyl-galactosamine residues. The Bidderian follicular cells showed the presence of beta-D-galactose and N-acetyl-D-glucosamine. In the extracellular matrix, alpha-mannose and alpha/beta-N-acetyl-galactosamine residues were detected. The ovarian zona pellucida showed alpha-L-fucose, N-acetyl-D-glucosamine, alpha/beta-N-acetyl-galactosamine residues, and alpha-mannose and N-acetyl-D-glucosamine residues were detected in the follicular cells. Thus, the zona pellucida in both organs contains N-acetyl-D-glucosamine, and alpha/beta-N-acetyl-galactosamine residues. alpha-L-fucose residues were detected in the zona pellucida of both organs, using different lectins. Considering that beta-D-galactose residue was absent from ovary but present in the Bidder's organ, this sugar residue may play an important role in follicle development, blocking the Bidderian follicles and preventing further development of the Bidder's organ into a functional ovary.

scholarly journals STUDI HISTOKIMIA LEKTIN TERHADAP JENIS DAN DISTRIBUSI GLIKOKONJUGAT ABOMASUM KERBAU RAWA (Bubalus bubalis) KALIMANTAN SELATAN

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Anni Nurliani ◽  
Teguh Budi Pitojo ◽  
Dwi Liliek Kusindarta
Keyword(s):  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Ricinus Communis ◽  
Periodic Acid ◽  
Bubalus Bubalis ◽  
Soybean Agglutinin ◽  
Con A ◽  
Periodic Acid Schiff ◽  
Ulex Europaeus ◽  
Ricinus Communis Agglutinin

Penelitian ini bertujuan mengkaji efisiensi pencernaan kerbau rawa dengan mengidentifikasi jenis dan distribusi glikokonjugat  pada daerah abomasum kerbau rawa. Enam ekor kerbau rawa jantan >2,5 tahun dan berat badan 300-400 kg digunakan dalam penelitian ini. Sampel diperoleh dari rumah potong hewan (RPH) Kabupaten Banjar, Kalimantan Selatan. Setiap bagian abomasum meliputi kardiak, fundus, dan pilorus diambil untuk pengamatan mikroskopis dengan pewarnaan hematoksilin-eosin (HE) dan alcian blue-periodic acid schiff (AB-PAS). Residu gula glikokonjugat pada abomasum dideteksi dengan pewarnaan histokimia lektin dengan menggunakan wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin agglutinin (Con A), ulex europaeus agglutinin (UEA), dan soybean agglutinin (SBA). Hasil penelitian menunjukkan bahwa daerah kardiak mengandung glikokonjugat D manosa/D glukosa, D galaktosa, dan N asetilglukosamin.  Daerah fundus mengandung D manosa/D glukosa, D galaktosa, L fukosa, N asetilglukosamin, dan N asetilgalaktosamin. Daerah pilorus mengandung glikokonjugat L fukosa dan N asetilglukosamin. Pola reaktivitas daerah kardiak, fundus, dan pilorus kerbau rawa terhadap pewarnaan histokimia lektin memiliki pola yang berbeda dengan ruminansia lain. Jenis glikokonjugat yang dimiliki oleh kerbau rawa tersebut diduga berkaitan dengan fungsi peningkatan kemampuan efisiensi pencernaan kerbau rawa. Setiap bagian abomasum kerbau rawa memiliki jenis glikokonjugat yang spesifik dengan pola distribusi khas sesuai dengan fungsinya.

Echinostoma caproni and E. trivolvis alter the binding of glycoconjugates in the intestinal mucosa of C3H mice as determined by lectin histochemistry

1993 ◽  
Vol 67 (3) ◽  
pp. 179-188 ◽  
Author(s):  
T. Fujino ◽  
B. Fried
Keyword(s):  
Ricinus Communis ◽  
Villous Atrophy ◽  
Lectin Binding ◽  
Goblet Cells ◽  
Lectin Histochemistry ◽  
Dolichos Biflorus ◽  
Echinostoma Caproni ◽  
Ulex Europaeus ◽  
C3h Mice ◽  
Small Intestines

AbstractMouse (C3H) mucosal glycoconjugates were examined in normal small intestines and intestines infected with Echinostoma caproni, or E. trivolvis using six different fluorescein-conjugated lectins: Triticum, vulgaris agglutinin (WGA), Ulex europaeus agglutinin I (UEA-I), Ricinus communis agglutinin I (RCA-I). Glycine max soybean agglutinin (SBA), Dolichos biflorus agglutinin (DBA), and Arachis hypogaeu peanut agglutinin (PNA). The expression of lectin-binding sites and the intensity of the binding of lectins in the mouse small intestines were changed by infection with the echinostomes. Specific differences in the reaction to glycoproteins were clearly observed between the mouse intestines infected with E. caproni and those infected with E. trivolvis. In E. caproni infection, binding of most of the lectins to the villi was remarkably reduced in accord with the villous atrophy and loss of goblet cells. In contrast, in E. trivolvis infection, the binding of WGA, RCA-I and DBA was reduced in the microvillar surfaces, but binding of UEA-I and SBA were unchanged compared to the control intestines. The lectin binding to goblet cells in E. trivolvis-infected mice mostly increased. These observations may reflect the marked increase in goblet cells and the less severe damage in the villi of E. trivolvis infection compared to E. caproni infection. Most of the glycoconjugates were slightly reduced in the hyperplastic crypts except for N-acetyl glucosamine. It is possible that glucose metabolism in the host intestines infected with E. trivolvis was activated. resulting in an increase in the rate of mucin synthesis as well as qualitative changes in mucus, thereby mediating the expulsion of the worms.

scholarly journals Genital Lesions following Long-term Administration of Clenbuterol in Female Pigs

1994 ◽  
Vol 31 (1) ◽  
pp. 82-92 ◽  
Author(s):  
B. Biolatti ◽  
M. Castagnaro ◽  
E. Bollo ◽  
S. Appino ◽  
G. Re
Keyword(s):  
Estrogen Receptors ◽  
Ricinus Communis ◽  
Lectin Histochemistry ◽  
Reproductive Organs ◽  
Corpora Lutea ◽  
Large White ◽  
Serum Progesterone ◽  
Ulex Europaeus ◽  
Term Administration ◽  
Theca Interna

Pathologic findings, lectin histochemistry, and nuclear estrogen receptors were studied in the reproductive organs of gilts treated with clenbuterol. A ration containing 1 ppm of clenbuterol was fed for 40 days to four Landrace x Large white, 9-month-old gilts, weighing 134 to 172 kg at slaughter (gilt Nos. 5–8). Four gilts (Nos. 1–4) served as controls. Treated animals had macroscopic lesions characterized by microcystic ovaries and uterine atrophy. Histopathologic lesions included atretic degeneration of many ovarian follicles, complete absence of functional corpora lutea, a reduction in the number of endometrial glands, and a decrease in cytoplasmic volume of endometrial and glandular epithelial cells. In ovaries, uterus, and vagina lectin histochemistry, performed with thirteen different biotinylated lectins, revealed a different staining distribution between control and treated gilts. The binding pattern of Ricinus communis agglutinin-1 (RCA-I) and -II (RCA-II) in the ovaries of control gilts, displayed labeling of cytoplasm in theca interna cells of Graafian follicles. There was no labeling of the same cells in treated gilts. Labeling patterns with Griffonia simplicifolia agglutinin-I (GS-I), Phaseolus vulgaris agglutinin (PHA), RCA-I and RCA-II documented a difference in the vascularis of the theca interna between Graafian follicles of control and treated gilts. The GS-1 and Ulex europaeus agglutinin-I (UEA-I) binding patterns in uterus and vagina of treated gilts when compared to control gilts suggested that there was a block of the cycling activity in the proliferative stage. Immunohistochemical staining for estrogen receptors in the endometrium was positive in all but one treated gilts, and negative to weakly positive in control gilts. Serum progesterone concentrations were decreased in treated animals when compared to control: estradiol concentrations were similar in both group of gilts. Cystic ovaries, uterine atrophy, and reduction in progesterone concentrations suggested that clenbuterol changed ovarian hormonal activity in treated animals.

Swainsonine Toxicosis Mimics Lectin Histochemistry of Mannosidosis

1985 ◽  
Vol 22 (4) ◽  
pp. 311-316 ◽  
Author(s):  
J. Alroy ◽  
U. Orgad ◽  
A. A. Ucci ◽  
V. E. Gavris
Keyword(s):  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Ricinus Communis ◽  
Lectin Histochemistry ◽  
Dolichos Biflorus ◽  
Ulex Europaeus ◽  
Lectin Staining ◽  
Astragalus Lentiginosus ◽  
Cytoplasmic Vacuoles ◽  
Ulex Europaeus Agglutinin I

Cells affected by locoweed (Astragalus lentiginosus) and Swainsona galegifolia toxicosis or mannosidosis exhibit similarities in their catabolism of N-linked glycoproteins and accumulation of cytoplasmic vacuoles. We used nine different biotinylated lectins as histochemical markers for specific sugars and avidin-biotin-peroxidase complex as a visualant to study the cells affected with these conditions. Since locoweed and Swainsona spp block mannosidase activity, we expected a similar lectin staining pattern in cells under these conditions as that seen in mannosidosis. Concanavalia ensiformis agglutinin, wheat germ agglutinin and succinyl wheat germ agglutinin stained the undegraded glycoproteins and oligosaccharides stored in the lysosomes of affected cells in all three conditions. Bandeirea simplicifolia-I, Dolichos biflorus agglutinin, peanut agglutinin, Ricinus communis agglutinin-I, soybean agglutinin and Ulex europaeus agglutinin-I did not stain any of these cells. These results indicate that in all three conditions there is an accumulation of undegraded oligosaccharides that contain α-mannosyl and β-N-acetyl glucosamine residues which are revealed by lectin staining in the vacuoles of all affected cells.

Ovary of the southern hairy-nosed wombat (Lasiorhinus latifrons): its divergent structural organisation

2019 ◽  
Vol 31 (9) ◽  
pp. 1457
Author(s):  
William G. Breed ◽  
Eleanor J. Peirce ◽  
Chris M. Leigh
Keyword(s):  
Zona Pellucida ◽  
Cycle Length ◽  
Periodic Acid ◽  
Oestrous Cycle ◽  
Interstitial Tissue ◽  
Cortical Region ◽  
Periodic Acid Schiff ◽  
Structural Organisation ◽  
Positive Material ◽  
Theca Interna

The organisation of the ovarian interstitial tissue in the southern hairy-nosed wombat Lasiorhinus latifrons was investigated. Unlike in most other marsupials, the outer cortical region of the ovary contains abundant luteinised interstitial tissue that largely occurs in discrete lobules, many of which contain a localised area of non-cellular, highly eosinophilic and periodic acid-Schiff-positive material. The findings suggest that the latter arises from the zona pellucida that surrounded the oocyte in growing follicles and that the luteinised interstitial tissue thus developed from transformed theca interna of degenerated atretic follicles. It is hypothesised that this tissue synthesises and secretes progestogens, which may result in the long, and variable, oestrous cycle length that has been found to occur in this species.

Modification of the outer surface of Erwinia carotovora ssp. atroseptica by the phytoalexin rishitin

1985 ◽  
Vol 31 (12) ◽  
pp. 1108-1112 ◽  
Author(s):  
W. M. Robertson ◽  
G. D. Lyon ◽  
C. E. Henry
Keyword(s):  
Outer Surface ◽  
Ricinus Communis ◽  
Periodic Acid ◽  
Membrane Integrity ◽  
Fluorescein Isothiocyanate ◽  
Erwinia Carotovora ◽  
Limulus Polyphemus ◽  
Dolichos Biflorus ◽  
Ulex Europaeus

The phytoalexin rishitin (500 μg/mL) modified the wall of Erwinia carotovora ssp. atroseptica by removing an outer layer and causing holes to appear through which the cell contents were extruded. A lower concentration of rishitin (300 μg/mL) produced effects on the cytoplasm visible by electron microscopy but no obvious differences in membrane integrity, although sections stained with the periodic acid – thiosemicarbazide – silver proteinate reaction showed a reduction in the intensity with which the carbohydrate layer in the outer membrane was stained which was similar to that found at the higher concentration (500 μg/mL). Labelling rishitin-treated cells with cationized ferritin showed the presence of negative charges on the outer surface of all cells treated with 500 μg rishitin/mL which were absent on nontreated cells and present in only a few cells treated with 300 μg/mL. After incubation of E. carotovora ssp. atroseptica with rishitin, cells bound to fluorescein isothiocyanate labelled lectins from Ricinus communis, Solanum tuberosum, and Triticum vulgaris and rhodamine-labelled avidin; cells of E. carotovora spp. atroseptica treated with ethanol were not labelled. Rishitin-treated cells did not bind to lectins from Arachis hypogaea, Dolichos biflorus, Glycine max, Limulus polyphemus, or Ulex europaeus. These results suggest that the phytoalexin rishitin can modify existing sites or expose previously blocked sites which may be involved in host–pathogen recognition, and may therefore indicate a reinforcement of other recognition processes in vivo.

scholarly journals CORRELATED LIGHT AND ELECTRON MICROSCOPE OBSERVATIONS ON GLYCOPROTEIN-CONTAINING GLOBULES IN THE FOLLICULAR CELLS OF THE THYROID GLAND OF THE RAT

1965 ◽  
Vol 13 (4) ◽  
pp. 286-295 ◽  
Author(s):  
HUBERTA E. VAN HEYNINGEN
Keyword(s):  
Electron Microscope ◽  
Thyroid Gland ◽  
Toluidine Blue ◽  
Fine Particles ◽  
Periodic Acid ◽  
Follicular Cells ◽  
Periodic Acid Schiff ◽  
Intense Staining ◽  
Thin Sections ◽  
Rat Thyroid

Two carbohydrate staining techniques were applied to sections of rat thyroid gland: periodic acid-silver methenamine to thin sections for electron microscopy, and periodic acid-Schiff to thick sections for light microscopy. The latter were compared with adjacent thin sections for identificatoin with the electron microscope. Two types of globules in thyroid follicular cells stained with both methods. Globules of the first type are relatively large (usually 0.5 to 3 µ) with electron opacity very similar to that of follicular colloid; when stained with toluidine blue they have the same gray shade as follicular colloid. These similarities suggest that their periodic acid reactivity is due to the same glycoprotein as that of follicular colloid, namely thyroglobulin, and that the origin of these "intracellular colloid droplets" is the colloid in the lumen. The second type comprises medium-sized (usually 0.1 to 1 µ) fairly electron opaque globules having fine particles (~70 Å) dispersed in their matrices and sometimes containing membrane fragments or other irregularities; when stained with toluidine blue these globules stand out dark blue. Although their periodic acid reactivity indicates that these globules also contain glycoprotein, their ultrastructure and staining characteristics suggest that their composition differs from colloid. It is possible that they represent enzyme or zymogen-containing granules. A third type of globule, which on account of its intense staining in some periodic acid silver methenamine preparations could perhaps also be periodic acid reactive, concerns small (usually 0.02 to 0.2 µ) globules, mainly accumulated beneath the apical border of the cell. These globules, known as "apical vesicles," are believed to contain the not-yet-iodinated precursor of thyroglobulin.

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

Detection of Carriers in Glanzmann's Thrombasthenia

1983 ◽  
Vol 49 (03) ◽  
pp. 182-186
Author(s):  
G T E Zonneveld ◽  
E F van Leeuwen ◽  
A Sturk ◽  
J W ten Cate
Keyword(s):  
Bleeding Time ◽  
Periodic Acid ◽  
Type I ◽  
Clot Retraction ◽  
Periodic Acid Schiff ◽  
Glanzmann’S Thrombasthenia ◽  
Aggregation Response ◽  
Glanzmann's Thrombasthenia ◽  
Sds Page ◽  
Reduced State

SummaryQuantitative glycoprotein (GP) analysis of whole platelets or platelet membranes was performed by SDS-polyacrylamide gelelectrophoresis (SDS-PAGE) and periodic acid Schiff staining in the families of two unrelated Glanzmann’s thrombasthenia (GT) patients. Each family consisted of two symptom free parents, a symptom free daughter and a GT daughter. All symptom free members had a normal bleeding time, clot retraction and platelet aggregation response to adenosine 5’-diphosphate (ADP), collagen and adrenalin. Platelet Zw* antigen was normally expressed in these subjects. GT patiens, classified as a type I and II subject, showed reduced amounts of GP lib and of GP nia. Analysis of isolated membranes in the non-reduced state, however, showed that the amount of GP Ilia was also reduced in three of the four parents, whereas one parent (of the GT type I patient) and the two unaffected daughters had normal amounts of GP Ilia. Quantitative SDS-PAGE may therefore provide a method for the detection of asymptomatic carriers in GT type I and II.

Swim bladder mycosis in farmed rainbow trout Oncorhynchus mykiss caused by Phoma herbarum and experimental verification of pathogenicity

2020 ◽  
Vol 138 ◽  
pp. 237-246 ◽  
Author(s):  
J Řehulka ◽  
A Kubátová ◽  
V Hubka
Keyword(s):  
Rainbow Trout ◽  
Histopathological Examination ◽  
Periodic Acid ◽  
Bladder Wall ◽  
Swim Bladder ◽  
Cell Reactivity ◽  
Periodic Acid Schiff ◽  
Posterior Portion ◽  
Hepatic Congestion ◽  
Phoma Herbarum

In this study, spontaneous swim bladder mycosis was documented in a farmed fingerling rainbow trout from a raceway culture system. At necropsy, the gross lesions included a thickened swim bladder wall, and the posterior portion of the swim bladder was enlarged due to massive hyperplasia of muscle. A microscopic wet mount examination of the swim bladder contents revealed abundant septate hyphae, and histopathological examination showed periodic acid-Schiff-positive mycelia in the lumen and wall of the swim bladder. Histopathological examination of the thickened posterior swim bladder revealed muscle hyperplasia with expansion by inflammatory cells. The causative agent was identified as Phoma herbarum through morphological analysis and DNA sequencing. The disease was reproduced in rainbow trout fingerlings using intraperitoneal injection of a spore suspension. Necropsy in dead and moribund fish revealed extensive congestion and haemorrhages in the serosa of visceral organs and in liver and abdominal serosanguinous fluid. Histopathological examination showed severe hepatic congestion, sinusoidal dilatation, Kupffer cell reactivity, leukostasis and degenerative changes. Fungi were disseminated to the liver, pyloric caeca, kidney, spleen and heart. Although infections caused by Phoma spp. have been repeatedly reported in fish, species identification has been hampered by extensive taxonomic changes. The results of this study confirmed the pathogenicity of P. herbarum in salmonids by using a reliably identified strain during experimental fish infection and provides new knowledge regarding the course of infection.

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