scholarly journals Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR

2021 ◽  
Vol 63 ◽  
Author(s):  
João Augusto Franco Leonel ◽  
Bruna Tannihão ◽  
Julia Assis Arantes ◽  
Geovanna Vioti ◽  
Julia Cristina Benassi ◽  
...  
Keyword(s):  
Leishmania Infantum ◽  
Equus Caballus ◽  
Blood Samples ◽  
Equus Asinus

scholarly journals Apicomplexan Protozoa Responsible for Reproductive Disorders: Occurrence of DNA in Blood and Milk of Donkeys (Equus asinus) and Minireview of the Related Literature

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 111
Author(s):  
Stefania Perrucci ◽  
Lisa Guardone ◽  
Iolanda Altomonte ◽  
Federica Salari ◽  
Simona Nardoni ◽  
...  
Keyword(s):  
Animal Species ◽  
Neospora Caninum ◽  
Reproductive Disorders ◽  
Theileria Equi ◽  
Blood Samples ◽  
Related Literature ◽  
Milk Samples ◽  
Equus Asinus ◽  
Time Periods ◽  
Blood Specimens

Donkeys may be susceptible to many pathological agents and may act as carriers of pathogens for other animal species and humans. This study evaluated the occurrence of potentially abortifacient apicomplexan protozoa DNA in blood and milk samples collected at different time periods during lactation (1, 6, and 10 months) from 33 healthy dairy jennies. A total of 73 blood and 73 milk samples were used for DNA extraction and analysis. Blood specimens from 11/33 (33%) jennies scored positive for Theileria equi, while milk samples scored negative. Blood and milk of 3/33 jennies yielded DNA of Toxoplasma gondii at 6 months (n. 1) and 10 months (n. 2) after parturition. Neospora caninum DNA was found in four milk and in five blood samples only at one month after parturition. This study is the first report about the presence of N. caninum DNA in milk of naturally infected jennies. Moreover, the excretion of N. caninum DNA in some of these jennies at 30 days from the parturition may suggest a possible occurrence of an endogenous cycle, while the presence of T. gondii DNA in the milk collected at 6 and 10 months after parturition may be suggestive of a discontinuous excretion.

scholarly journals Biometria de pontes de miocárdio em muares (Equus caballus x Equus asinus – Linnaeus 1758)

Biotemas ◽  
2011 ◽  
Vol 22 (3) ◽  
Author(s):  
André Luiz C. Ribeiro ◽  
Renato S. Severino ◽  
Ricardo R. Guerra ◽  
Phelipe Oliveira Favaron ◽  
Horácio L. P. Tommasi Junior ◽  
...  
Keyword(s):  
Equus Caballus ◽  
Equus Asinus

scholarly journals SYBR green-based detection of Leishmania infantum DNA using peripheral blood samples

2014 ◽  
Vol 40 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Mehrdad Ghasemian ◽  
Mohammad Javad Gharavi ◽  
Lame Akhlaghi ◽  
Mehdi Mohebali ◽  
Ahmad Reza Meamar ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Leishmania Infantum ◽  
Sybr Green ◽  
Blood Samples

Evaluation of a novel dried blood spot collection device (HemaSpot™) to test blood samples collected from dogs for antibodies to Leishmania infantum

2014 ◽  
Vol 205 (1-2) ◽  
pp. 338-342 ◽  
Author(s):  
Alexa C. Rosypal ◽  
Leanne D. Pick ◽  
Jaime O. Esquivel Hernandez ◽  
David S. Lindsay
Keyword(s):  
Leishmania Infantum ◽  
Dried Blood Spot ◽  
Blood Samples ◽  
Collection Device ◽  
Blood Spot

scholarly journals Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

2018 ◽  
Vol 66 (2) ◽  
pp. 106
Author(s):  
Z. BILGIN ◽  
N. TURAN ◽  
H. YILMAZ ◽  
E. FERROGLIO ◽  
E. TUZER
Keyword(s):  
Weight Loss ◽  
Leishmania Infantum ◽  
Clinical Symptoms ◽  
Skin Lesions ◽  
Leishmania Infection ◽  
Kinetoplast Dna ◽  
Blood Samples ◽  
Mediterranean Countries ◽  
Pcr Diagnosis ◽  
Serious Disease

Leishmania infantum is widespread in Mediterranean countries including Turkey and can cause a serious disease in both humans and dogs. Dogs are considered to be its main reservoirs. The current epidemiological study was carried out in Istanbul for detection of leishmaniosis among dogs. A total of 246 dogs were included in the study. Twenty one (8.54%) blood samples were found to be positive based on PCR diagnosis, using primers specific for the kinetoplast DNA of Leishmania. Infection rate was 6.51% in 169 dogs living in shelters and 12.99% in 77 client-owned dogs. The rate was significantly (p<0.001) higher in the dogs (37.93%) presenting one or more clinical symptoms which may be attributable to leishmaniosis, than in the asymptomatic dogs (4.61%). Eleven (52.4%) of 21 PCR positive dogs presented clinical symptoms whereas the rest of the dogs (47.62%) were asymptomatic. The major clinical sign in PCR positive animals was dermatological problems. Amongst PCR positive dogs, skin lesions were present in 11, weight loss in 2, lymphadenopathy in 2, epistaxis in 2 and lethargy in 2 dogs.

Intestinal ciliates (Alveolata, Ciliophora) in Brazilian domestic horses (Equus caballus L.) and a review on the ciliate communities associated with horses around the world

Zootaxa ◽  
2019 ◽  
Vol 4585 (3) ◽  
pp. 478
Author(s):  
FRANCIANE CEDROLA ◽  
SUYANE BORDIM ◽  
MARTA D’AGOSTO ◽  
ROBERTO JÚNIO PEDROSO DIAS
Keyword(s):  
South America ◽  
Equus Caballus ◽  
American Continent ◽  
Geographic Locations ◽  
Equus Asinus ◽  
The Family ◽  
The World ◽  
Tapirus Terrestris ◽  
Domestic Horses ◽  
First Time

The aims of this study were to identify and quantify the gastrointestinal ciliates from Brazilian domestic horses, to compare the data obtained with previous studies and to review data on ciliate communities associated with horses around the world. We used eight domestic horses, kept under extensive management. Fecal samples were obtained immediately after defecation of animals and fixed in 18.5% formaldehyde. We identify 24 species of endosymbiotic ciliates, distributed in two subclasses, two orders, five families and 13 genera. The ciliate composition was similar among the animals analyzed. The family Blepharocorythidae presented the higher prevalence and the family Buetschliidae was observed in greater abundance and density. Among the species identified, Cycloposthium dentiferum was observed for the first time in Central and South America and Bundleia elongata, B. inflata, B. piriformis, B. vorax and Cycloposthium psicauda, were observed for the first time in the American continent. In addition, the species Buissonella tapiri, which was originally discovered in fecal contents of the American tapir (Tapirus terrestris) in Brazil was observed for the first time associated to Equus caballus. The review on the ciliate communities associated with horses around the world demonstrated that the equine ciliates are a very diversified group with representatives in two subclasses, Suctoria and Trichostomatia, totaling 78 species distributed in three orders and six families, having been registered in different equine hosts (Equus asinus, Equus caballus and Equus quagga) and geographic locations (USA, China, Russia, Taiwan, Mexico, Cyprus, Turkey, Kyrgyzstan and Brazil). 

Preliminary findings in the rolling behavior of Mules (Equus caballus x Equus asinus)

2021 ◽  
Author(s):  
Marina P.F. Luz ◽  
Caroline M. Maia ◽  
José Nicolau P. Puoli Filho
Keyword(s):  
Equus Caballus ◽  
Equus Asinus

scholarly journals Habronema e Draschia: alguns dados sobre infecções em eqüinos, asininos e muares de alguns estados brasileiros

1996 ◽  
Vol 33 (2) ◽  
pp. 89
Author(s):  
Amália Verônica Mendes Silva ◽  
Claudia Freire de Andrade Moraes ◽  
Helio Martins de Araújo Costa
Keyword(s):  
Rio Grande ◽  
Equus Caballus ◽  
Minas Gerais ◽  
Equus Asinus ◽  
Habronema Muscae

Foram examinados os estômagos de 37 Equus caballus, 18 Equus asinus e 22 muares, procedentes do Estado do Maranhão, Piauí, Ceará, Rio Grande do Norte, Paraíba, Pernambuco, Minas Gerais e Goiás, para a pesquisa de espirurídeos e foram encontrados: Draschia megastoma (Rudolphi, 1819), Habronema muscae (Carter, 1861) e Habronema microstoma (Schneider, 1866). Foram consideradas as prevalências, as intensidades médias e as relações Macho/Fêmea para estes parasitos, nos três hospedeiros. A análise de variância mostrou não ocorrerem diferenças estatisticamente significativas entre as intensidades médias de H. muscae e H. microstoma, inclusive na comparação entre os hospedeiros.

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