SYBR green-based detection of Leishmania infantum DNA using peripheral blood samples

AkDNAPCR enzyme-linked immunosorbent assay (kDNAPCR-ELISA) for the diagnosis of human visceral leishmaniasis (HVL) was developed. The detection limit of the reaction, precision measurements, and cut-off of thekDNAPCR-ELISA were defined in a proof-of-concept phase. A reference strain ofLeishmania (Leishmania) infantumand a bank of 14 peripheral blood samples from immunocompetent patients with VL were characterized using techniques considered gold standards, and 11 blood samples obtained from healthy individuals of an endemic area were also assessed. Phase II evaluation determined the performance of the assay in peripheral blood samples from 105 patients with VL (adults and children), 25 patients withLeishmania/HIV coinfection, 40 healthy individuals, and 33 asymptomatic individuals living in endemic areas. ThekDNAPCR-ELISA exhibited satisfactory precision, with a detection limit of 0.07 fg of DNA fromL.(L.) infantumand 1 parasite/mL blood. The overall sensitivity of the assay for all groups studied was 100% (95% confidence interval [CI]: 97.1–100%), and the specificity was 95% (95% CI: 83.5–98.6%). ThekDNAPCR-ELISA was shown to be a useful tool for VL symptomatic and asymptomatic individuals diagnosis and its use in endemic countries may help monitor control interventions.

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Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro

Research in Veterinary Science ◽

10.1016/j.rvsc.2020.11.021 ◽

2021 ◽

Vol 134 ◽

pp. 58-63

Author(s):

Matheus Fujimura Soares ◽

Larissa Martins Melo ◽

Jaqueline Poleto Bragato ◽

Amanda de Oliveira Furlan ◽

Natália Francisco Scaramele ◽

...

Keyword(s):

Differential Expression ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Leishmania Infantum ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

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Evaluation of T cell-mediated antitumor immune response in liver tumors - an approach using tumor and peripheral blood samples

HPB ◽

10.1016/j.hpb.2018.06.207 ◽

2018 ◽

Vol 20 ◽

pp. S262

Author(s):

R. Martins ◽

C. Martín-Sierra ◽

P. Laranjeira ◽

A.M. Abrantes ◽

J.G. Tralhão ◽

...

Keyword(s):

Immune Response ◽

T Cell ◽

Peripheral Blood ◽

Liver Tumors ◽

Antitumor Immune Response ◽

Blood Samples

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Increased Density of Human Immunodeficiency Virus Type 1 Coreceptors CCR5 and CXCR4 on the Surfaces of CD4+ T Cells and Monocytes of Patients with Schistosoma mansoni Infection

Infection and Immunity ◽

10.1128/iai.71.11.6668-6671.2003 ◽

2003 ◽

Vol 71 (11) ◽

pp. 6668-6671 ◽

Cited By ~ 58

Author(s):

W. Evan Secor ◽

Amil Shah ◽

Pauline M. N. Mwinzi ◽

Bryson A. Ndenga ◽

Caroline O. Watta ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

T Cells ◽

Cell Surface ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Peripheral Blood ◽

Chemokine Receptors ◽

Blood Samples ◽

Immunodeficiency Virus

ABSTRACT Distribution of chemokine receptors CCR5 and CXCR4, which are also coreceptors for human immunodeficiency virus type 1 invasion of cells, was measured on the surfaces of CD4+ T cells and monocytes in peripheral blood samples from a group of Kenyan car washers. Patients with active schistosomiasis displayed higher cell surface densities of these receptors than did cured schistosomiasis patients.

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Evaluation of the tshr gene reveals polymorphisms associated with typical symptoms in primary congenital hypothyroidism

Journal of Pediatric Endocrinology and Metabolism ◽

10.1515/jpem-2015-0130 ◽

2016 ◽

Vol 29 (1) ◽

Author(s):

Erik Artur Cortinhas Alves ◽

Raissa Coelho Andrade ◽

Carlos Eduardo de Melo Amaral ◽

Milena Coelho Fernandes Caldato ◽

Adriana Maria Rocha Bastos ◽

...

Keyword(s):

Congenital Hypothyroidism ◽

Peripheral Blood ◽

Genomic Dna ◽

Gene Mutations ◽

Molecular Heterogeneity ◽

Coding Region ◽

Blood Samples ◽

Live Births ◽

Tshr Gene ◽

Inactivating Mutations

AbstractPrimary congenital hypothyroidism (PCH) has an incidence of approximately 1 in each 3000–4000 live births. In the last two decades, nearly 50 types of the distinct inactivating mutations have already been described in the coding region of the tshr gene. The aim of present study was to investigate tshr gene mutations in patients with primary congenital hypothyroidism, analyzing a sample of 106 patients that were diagnosed with PCH. Genomic DNA was isolated from peripheral blood samples, and 10 exons from the TSH receptor were automatically sequenced. Five nucleotide alterations (P52T, N187N, A459A, L645L, and D727E. N187N and D727E polymorphisms) were associated with positive medical history. In view of the clinical, biochemical and molecular heterogeneity of the etiology of the PCH, the study of polymorphisms is critical for investigating the possible associations with prevailing symptoms of this disorder.

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Associations between the Epithelial-Mesenchymal Transition Phenotypes of Circulating Tumor Cells and the Clinicopathological Features of Patients with Colorectal Cancer

Disease Markers ◽

10.1155/2017/9474532 ◽

2017 ◽

Vol 2017 ◽

pp. 1-6 ◽

Cited By ~ 7

Author(s):

Fengjie Wu ◽

Jun Zhu ◽

Yongjiang Mao ◽

Xiaomei Li ◽

Baoguang Hu ◽

...

Keyword(s):

Colorectal Cancer ◽

Peripheral Blood ◽

Epithelial Mesenchymal Transition ◽

Mesenchymal Cell ◽

Blood Samples ◽

Mesenchymal Transition ◽

Enrichment Technique ◽

Tumor Node Metastasis Stage ◽

Mesenchymal Cell Marker ◽

Circulating Tumor Microemboli

In this study, we identified CTCs using the previously reported CanPatrol CTC enrichment technique from peripheral blood samples of 126 patients with colorectal cancer (CRC) and found that CTCs could be classified into three subpopulations based on expression of epithelial cell adhesion molecule (EpCAM) (E-CTCs), the mesenchymal cell marker vimentin (M-CTCs), or both EpCAM and vimentin (biphenotypic E/M-CTCs). Circulating tumor microemboli (CTMs) were also identified in peripheral blood samples. Meanwhile, E-CTCs, M-CTCs, E/M-CTCs, and CTMs were detected in 76.98%, 42.06%, 56.35%, and 36.51% of the 126 patients, respectively. Interestingly, the presence of CTMs and each CTC subpopulation was significantly associated with blood lymphocyte counts and tumor-node-metastasis stage (P<0.001). Lymphocyte counts and the neutrophil-to-lymphocyte ratio (NLR) in patients lacking CTCs were significantly different from those in patients testing positive for CTMs and each CTC subpopulation (P<0.001). Our results indicate that tumor metastasis is more significantly associated with the presence of CTMs and M-CTCs than with other CTC subpopulations and suggest that EMT may be involved in CTC evasion of lymphocyte-mediated clearance.

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Development of Implantable Autologous Small-Calibre Vascular Grafts from Peripheral Blood Samples

Zentralblatt für Chirurgie - Zeitschrift für Allgemeine Viszeral- Thorax- und Gefäßchirurgie ◽

10.1055/s-0032-1315112 ◽

2013 ◽

Vol 138 (02) ◽

pp. 173-179 ◽

Cited By ~ 6

Author(s):

T. Aper ◽

O. Teebken ◽

A. Krüger ◽

A. Heisterkamp ◽

A. Hilfiker ◽

...

Keyword(s):

Peripheral Blood ◽

Vascular Grafts ◽

Blood Samples

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Earlier Positivity of Central-Venous- versus Peripheral-Blood Cultures Is Highly Predictive of Catheter-Related Sepsis

Journal of Clinical Microbiology ◽

10.1128/jcm.36.1.105-109.1998 ◽

1998 ◽

Vol 36 (1) ◽

pp. 105-109 ◽

Cited By ~ 198

Author(s):

François Blot ◽

Eric Schmidt ◽

Gérard Nitenberg ◽

Cyrille Tancrède ◽

Bernard Leclercq ◽

...

Keyword(s):

Central Venous Catheter ◽

Peripheral Blood ◽

Venous Catheter ◽

Blood Cultures ◽

Central Venous ◽

Indwelling Catheter ◽

Blood Samples ◽

Reference Center ◽

Reliable Tool ◽

Period Data

To diagnose catheter-related sepsis without removing the catheter, we evaluated the differential positivity times of cultures of blood drawn simultaneously from central venous catheter and peripheral sites. In a 450-bed cancer reference center, simultaneous central- and peripheral-blood cultures were prospectively performed for patients with suspicion of catheter-related sepsis over an 18-month period. Data for 64 patients for whom the same microorganisms were found when central- and peripheral-blood samples were cultured were retrospectively reviewed by two independent physicians blinded to the differential positivity time values in order to establish or refute the diagnosis of catheter-related sepsis. The diagnosis was established in 28 cases, refuted in 14, and indeterminate in the remaining 22. The differential positivity time was significantly greater for patients with catheter-related sepsis (P < 10−4). A cutoff limit of +120 min had 100% specificity and 96.4% sensitivity for the diagnosis of catheter-related sepsis. These results strongly suggest that measurement of the differential positivity time might be a reliable tool facilitating the diagnosis of catheter-related sepsis in patients with an indwelling catheter.

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