scholarly journals Seroprevalence of antibodies to Neospora caninum in dogs in the state of Alagoas, Brazil

2012 ◽  
Vol 21 (3) ◽  
pp. 287-290 ◽  
Author(s):  
Maria Evódia de Sousa ◽  
Wagnner José Nascimento Porto ◽  
Pedro Paulo Feitosa de Albuquerque ◽  
Orestes Luiz de Souza Neto ◽  
José Wilton Pinheiro Júnior ◽  
...  
Keyword(s):  
Risk Factors ◽  
Rural Areas ◽  
Urban Areas ◽  
Neospora Caninum ◽  
Antibody Test ◽  
The State ◽  
Factors Associated ◽  
Univariate Analyses ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

This study investigated occurrences of antibodies to anti-Neospora caninum in dogs in the state of Alagoas, Brazil, and the risk factors associated with the infection. To investigate antibodies against N. caninum, an indirect immunofluorescence antibody test (IFAT) was used. To identify risk factors associated with infection by N. caninum, univariate analyses on variables of interest were conducted. The study analyzed 237 samples from dogs of both sexes and different breeds and ages, both from rural and from urban areas. Ten animals (4.2%) were seropositive: five (4.8%) from rural areas and five (3.8%) from urban areas. Regarding sex, five (4.0%) were male and five (4.5%) were female. In relation to breed, four animals of undefined breed and six of defined breed were positive. The ages of the seropositive animals ranged from two months to eleven years. Results show there are no significant associations between seropositivity and the variables analyzed. Results indicate neosporosis must be included in differential diagnosis of dog neurological disorders.

scholarly journals Toxoplasma gondii and Neospora caninum in dogs from the state of Tocantins: serology and associated factors

2015 ◽  
Vol 24 (4) ◽  
pp. 475-481 ◽  
Author(s):  
Juliana Macedo Raimundo ◽  
Andresa Guimarães ◽  
Larissa Martins de Brito Moraes ◽  
Lucélia Azevedo Santos ◽  
Leandro Lopes Nepomuceno ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rural Areas ◽  
Urban Areas ◽  
Neospora Caninum ◽  
The State ◽  
Igg Antibodies ◽  
Factors Associated ◽  
Rural And Urban Areas ◽  
Rural And Urban ◽  
Urban And Rural Areas

Abstract This study investigated occurrences of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies by means of the enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence assay (IFAT), along with risk factors associated with toxoplasmosis and neosporosis, in 204 dogs from urban and rural areas of the municipality of Araguaína, state of Tocantins, Brazil. One hundred and thirty samples (63.7%) were positive for T. gondii using ELISA: 57.1% and 70.7% in the urban and rural areas, respectively. The seropositivity frequency for T. gondii observed through IFAT was 57.4%, distributed between rural and urban areas as 62.6% and 52.4%, respectively. The factors associated with canine toxoplasmosis were age and breed (p<0.05). In relation to N. caninum, 88 samples (43.1%) were positive, according to ELISA, distributed as 42.9% in urban areas and 43.3% in rural areas. Anti - N. caninum antibodies were detected through IFAT in 62 dogs (30.4%), distributed as 31.3% and 29.5% between rural and urban areas, respectively. Age and breed were associated with neosporosis occurrence (p<0.05) by IFAT. This study provides the first detection of IgG antibodies for canine toxoplasmosis and neosporosis in the state of Tocantins and highlights the importance of dogs in the epidemiological chain of these diseases.

scholarly journals Neospora caninum seroprevalence and risk factors for ewes from Santa Catarina Plateau, Brazil

2014 ◽  
Vol 35 (5) ◽  
pp. 2591 ◽  
Author(s):  
Anderson Barbosa de Moura ◽  
Marcelo Felipe Güths ◽  
Juliana Antunes Farias ◽  
Antonio Pereira de Souza ◽  
Amélia Aparecida Sartor ◽  
...  
Keyword(s):  
Risk Factors ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Economic Losses ◽  
Reproductive Disorders ◽  
Santa Catarina ◽  
Chi Square ◽  
Animal Management ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Neospora caninum can cause reproductive problems and economic losses in sheep flock. In order to evaluate N. canium seroprevalence and to identify risk factors, blood samples were collected from 1,308 ewes at 92 properties in 30 municipalities of Santa Catarina Plateau, southern Brazil. The antibodies against N. caninum were detected by Indirect Immunofluorescence Antibody Test (IFAT, ≥1:50) and the seropositive were titrated in serial twofold up to a maximum dilution. Data concerning animal management and information about reproductive problems were obtained from questionnaires. The results were tabulated and analyzed using the Fisher’s exact and Chi-Square tests (P<0.05). Among the 1,308 animals evaluated, 92 (7%) were seropositive to N. caninum. The titers observed varied from 1:50 to 1:3200. In 39 (42.4%) properties there was at least one infected animal. Breed (P=0.0249) and contact with cattle (P=0.0491) showed correlation with the prevalence of N. caninum. No association was found between N. caninum serology and reproductive disorders.

scholarly journals Occurrence and risk factors associated with infection by Toxoplasma gondii in goats in the State of Alagoas, Brazil

2011 ◽  
Vol 44 (2) ◽  
pp. 157-162 ◽  
Author(s):  
Giulliano Aires Anderlini ◽  
Rinaldo Aparecido Mota ◽  
Eduardo Bento Faria ◽  
Erika Fernanda Torres Samico Fernandes Cavalcanti ◽  
Rômulo Menna Barreto Valença ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Antibody Test ◽  
The State ◽  
Disease Occurrence ◽  
Toxoplasma Gondii Infection ◽  
Placental Remnants ◽  
Breeding Farms ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

INTRODUCTION: Goats are considered very susceptible to infection by Toxoplasma gondii and when this occurs during pregnancy, it may cause fetal death with subsequent fetal resorption, abortion, mummification, stillborn or the birth of weak goats. The objective of this work was to determine the occurrence of and identify risk factors for T. gondii infection in goats in different mesoregions in the State of Alagoas. METHODS: The research was conducted on 24 goat breeding farms in 10 municipalities. A total of 454 blood samples were examined for anti-T. gondii antibodies by indirect immunofluorescence antibody test. To evaluate the risk factors of toxoplasmosis in goats, questionnaires were applied analyzing the farm's production system and nutritional, reproductive and sanitary management. RESULTS: Disease occurrence was 39% with 95.8% of farms presenting seropositive animals. Significant associations were observed for mesoregion (OR = 0.23; 95%CI = 0.09 - 0.57), age (OR = 0.36; 95%CI = 0.20 - 0.64), semi-intensive herd management (OR = 8.70; 95%CI = 1.87 - 40.43), access of cats to water provided for goats (OR = 3.38; 95%CI = 1.89-6.02) and cats feeding on placental remnants (OR = 2.73; 95%CI = 1.38 - 5.40). CONCLUSIONS: Toxoplasma gondii infection is disseminated in the State of Alagoas and the adoption of a program that monitors infection foci is required to reduce the risk factors identified in the present study.

scholarly journals Risk factors for Toxoplasma gondii and Neospora caninum seropositivity in buffaloes in Paraiba State, Brazil

2015 ◽  
Vol 24 (4) ◽  
pp. 459-463 ◽  
Author(s):  
Arthur Willian de Lima Brasil ◽  
Roberta Nunes Parentoni ◽  
Thais Ferreira Feitosa ◽  
Camila de Sousa Bezerra ◽  
Vinicius Longo Ribeiro Vilela ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Odds Ratio ◽  
Indirect Fluorescent Antibody Test ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Management Systems ◽  
Intensive Management ◽  
Factors Associated

Abstract The aims of this survey were to determine the frequency of anti-Toxoplasma gondii and anti-Neospora caninum antibodies and to identify the risk factors associated with seropositivity among buffaloes in the state of Paraíba, Brazil. This survey included 136 buffaloes belonging to 14 herds. To detect anti-T. gondii and anti-N. caninum antibodies, the indirect fluorescent antibody test (IFAT) was used. Among the 136 samples analyzed, 17 (12.5%) were positive for anti-T. gondii antibodies with titers ranging from 64 to 1,024, and 26 (19.1%) for anti-N. caninum with titers from 200 to 1,600. Animals seropositive for both T. gondii and N. caninum were found in 10 of the 14 herds (71.4%). Semi-intensive management systems (odds ratio = 2.99) and presence of pigs (odds ratio = 4.33) were identified as risk factors for T. gondii and N. caninum, respectively. It can be suggested that T. gondii and N. caninum are widespread in buffaloes in Paraíba, and that additional surveys are needed in order to ascertain the importance of these agents for this species and for pigs, and the influence of the farming type on occurrences of seropositive animals.

scholarly journals Factors associated with the distribution of natural Toxoplasma gondii infection among equids in Northeastern Brazil

2019 ◽  
Vol 28 (2) ◽  
pp. 283-290 ◽  
Author(s):  
Alexandre Dias Munhoz ◽  
Monia Andrade Souza ◽  
Sonia Carmen Lopo Costa ◽  
Jéssica de Souza Freitas ◽  
Aísla Nascimento da Silva ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rural Areas ◽  
Urban Areas ◽  
Linear Models ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Northeastern Brazil ◽  
Domestic Cats ◽  
Serum Samples ◽  
Factors Associated

Abstract The aim of this study was to determine the frequency and factors associated with Toxoplasma gondii in naturally infected equids in northeastern Brazil. Serum samples from 569 equids (528 horses, 8 mules and 33 donkeys) were subjected to the indirect fluorescent antibody test. Generalized linear models were used to evaluate associated factors. Among the 569 animals sampled, 118 (30.6%) living in rural areas and 14 (26.42%) in urban areas were seropositive (p>0.05). Seropositive animals were observed on 95% of the farms and in all the municipalities. Donkeys/mules as the host, presence of domestic cats and rats on the farm, ingestion of lagoon water and goat rearing remained in the final model as factors associated with infection. Preventive measures such as avoiding the presence of domestic cats close to rearing areas, pastures and sources of water for the animals should be adopted. The wide-ranging distribution of positive animals also indicated that infection in other domestic animals and in humans, through the contaminated environment, was possible. It should be highlighted that there was the possibility that donkeys and mules would continue to have detectable titers for longer, thus explaining the prevalence found. Further studies are needed to confirm this possibility.

scholarly journals Low prevalence of infection by Sarcocystis neurona in horses from the State of Alagoas, Brazil

2019 ◽  
Vol 28 (2) ◽  
pp. 298-302 ◽  
Author(s):  
Sandra Regina Fonseca de Araújo Valença ◽  
Müller Ribeiro-Andrade ◽  
Gastón Moré ◽  
Pedro Paulo Feitosa de Albuquerque ◽  
José Wilton Pinheiro Júnior ◽  
...  
Keyword(s):  
Risk Factors ◽  
Antibody Test ◽  
Sarcocystis Neurona ◽  
Animal Management ◽  
Serological Status ◽  
Potential Risk Factors ◽  
Low Exposure ◽  
Low Prevalence ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Abstract The aim of this study was to determine the prevalence of infection by Sarcocystis neurona in horses and identify potential risk factors. Were analyzed 427 samples from 36 farms in 21 municipalities in the Alagoas State, Brazil. Presence of anti-S. neurona antibodies was diagnosed by indirect immunofluorescence antibody test (IFAT) and was confirmed using the immunoblot test. Risk factors were assessed through investigative questionnaires on animal management on the farms. The prevalence of anti-S.neurona antibodies was 2.8% (confidence interval, CI: 1.5-4.9%) from IFAT and 1.6% (CI:0.8-3.34%) from immunoblot, and there were positive horses on 16.6% of the studied farms. None of the variables studied presented associations with serological status for S. neurona. This is the first report on infection by S. neurona in horses reared in Alagoas, Brazil showing a low exposure to S. neurona in this region, but with significant numbers of foci.

scholarly journals Toxoplasma gondii antibodies on domiciled cats from Lages municipality, Santa Catarina State, Brazil

2010 ◽  
Vol 19 (4) ◽  
pp. 268-269 ◽  
Author(s):  
Luciana Dalla Rosa ◽  
Anderson Barbosa de Moura ◽  
Natascha Trevisani ◽  
Alessandra Pereira Medeiros ◽  
Amélia Aparecida Sartor ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Rural Areas ◽  
Antibody Test ◽  
Southern Brazil ◽  
Santa Catarina ◽  
Factors Associated ◽  
Immunofluorescent Antibody ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

Sera were collected from 300 domiciled cats from the municipality of Lages, Southern Brazil, to determine the prevalence of Toxoplasma gondii antibodies and risk factors associated. Tests for T. gondii antibodies were performed using indirect immunofluorescent antibody test (IFAT). Positive reactions with titers >1:64 were found in 43 (14.33%) cats. A significant number of seropositive cats were >6 month old (p = 0.03758) and had access to the streets or/and rural areas (p = 0.04185). The results indicate that T. gondii is widespread in cats in Lages with a prevalence of 14.33%.

scholarly journals Risk factor analysis associated with Neospora caninum in dairy cattle in Western Brazilian Amazon

2021 ◽  
Vol 30 (1) ◽  
Author(s):  
Poliana de Jesus Souza Venturoso ◽  
Osvaldo Juliatti Venturoso ◽  
Gisele Glomba Silva ◽  
Maerle Oliveira Maia ◽  
Rute Witter ◽  
...  
Keyword(s):  
Risk Factors ◽  
Dairy Cattle ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Serum Samples ◽  
Present Survey ◽  
Caninum Infection ◽  
Factors Associated ◽  
And Control

Abstract Neospora caninum is considered to be one of the main causes of abortion among cattle. The present survey was conducted in the municipality of Rolim de Moura, Rondônia State, Brazil. A questionnaire that investigates the epidemiological aspects of neosporosis was used in the analysis of risk factors associated with the animal-level and herd-level prevalence in dairy cattle. A total of 416 bovine blood samples were collected from 30 farms, and N. caninum antibody levels were measured by Indirect Fluorescent Antibody Test (IFAT). Analysis of dairy cattle serum samples revealed the presence of anti-N. caninum antibodies to be 47.36% (n = 197). Risk factors associated with N. caninum infection were the management system and access locations of dogs. The results of the present survey indicated that infection of dairy cattle with N. caninum is widespread in the studied region of Western Amazon, which has implications for prevention and control of neosporosis in this region. Therefore, integrated control strategies and measures are recommended to prevent and control N. caninum infection in dairy cattle. In addition, direct contact between dairy cattle, dogs and wild animals, which can influence the epidemiology of neosporosis, should be investigated further.

scholarly journals Occurrences of anti-Toxoplasma gondiiand anti-Neospora caninum antibodies in Barbary sheep at Curitiba zoo, southern Brazil

2014 ◽  
Vol 23 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Vivien Midori Morikawa ◽  
Cristina Kraemer Zimpel ◽  
Igor Adolfo Dexheimer Paploski ◽  
Maria do Carmo Custódio de Souza Hunold Lara ◽  
Eliana Monteforte Cassaro Villalobos ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Southern Brazil ◽  
Intermediate Hosts ◽  
Blood Samples ◽  
Ammotragus Lervia ◽  
Barbary Sheep ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Barbary sheep (Ammotragus lervia) have the potential to act as hosts of important infectious diseases, particularly zoonoses. Blood samples from 17 Barbary sheep at the Curitiba zoo were collected to evaluate occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies, tested using the indirect immunofluorescence antibody test (IFAT). Anti-T. gondii and anti-N. caninum antibodies were detected in 4/17 (23.5%) and 4/17 (23.5%) samples, respectively. The present study has shown that Barbary sheep at Curitiba zoo were exposed to T. gondii andN. caninum and therefore may act as intermediate hosts, spreading toxoplasmosis and neosporosis within and between species in shared areas.

scholarly journals Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil

2016 ◽  
Vol 25 (4) ◽  
pp. 418-422 ◽  
Author(s):  
Denise Amaral Gomes Nascimento ◽  
Rafael Felipe da Costa Vieira ◽  
Thállitha Samih Wischral Jayme Vieira ◽  
Roberta dos Santos Toledo ◽  
Katia Tamekuni ◽  
...  
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Antibody Test ◽  
Rural Settlement ◽  
Southern Brazil ◽  
Tick Bites ◽  
Serum Samples ◽  
Factors Associated ◽  
Borrelia Burgdorferi S.L ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Abstract The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Brazil. Eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. Immunofluorescence antibody test (IFAT) was performed on serum samples and positive samples were subjected to western blot (WB) analysis. Anti-B. burgdorferi antibodies were found in 4/87 (4.6%) humans, 26/83 (31.3%) dogs, and 7/18 (38.9%) horses by IFAT, with 4/4 humans also positive by WB. Ticks identified were mostly from dogs and included 45/67 Rhipicephalus sanguineus, 21/67 Amblyomma ovale, and 1/67 A. cajennense sensu lato. All (34/34) horse ticks were identified as A. cajennense s.l.. No significant association was found when age, gender, or presence of ticks was correlated to seropositivity to Borrelia sp. In conclusion, although anti-Borrelia antibodies have been found in dogs, horses and their owners from the rural settlement, the lack of isolation, molecular characterization, absence of competent vectors and the low specificity of the commercial WB kit used herein may have impaired risk factor analysis.

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