scholarly journals First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata)

2019 ◽  
Vol 28 (3) ◽  
pp. 489-492
Author(s):  
Mércia de Seixas ◽  
Alessandra Taroda ◽  
Sérgio Tosi Cardim ◽  
João Pedro Sasse ◽  
Thais Agostinho Martins ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Protozoan Parasite ◽  
18S Rrna Gene ◽  
Pcr Analysis ◽  
Rrna Gene ◽  
Cryptosporidium Spp ◽  
Potential Source ◽  
Wide Range ◽  
First Time

Abstract Cryptosporidium is a protozoan parasite with a wide range of hosts, including humans. However, only a few Cryptosporidium species have been described in birds (C. meleagridis, C. baileyi, C. galli and C. avium). The aim of this study was to investigate the occurrence of Cryptosporidium spp. in feces of eared doves (Zenaida auriculata), followed by molecular characterization of the parasite. A total of 196 animals of both sexes were trap-captured; the animals were culled and the intestinal contents were collected for DNA extraction. After extraction, a nested-PCR (nPCR), which amplifies a fragment of the 18S rRNA gene of Cryptosporidium spp., was performed. The amplicons obtained were purified and sequenced. PCR analysis revealed that 30 animals (15.3%) were positive for Cryptosporidium spp. There was no significant sex-dependent enrichment of Cryptosporidium occurrence (p > 0.05). Only 15 out of the 30 positive samples were successfully sequenced and their species determined, of which, 13 (86.7%) and 2 (13.3%) were C. meleagridis and C. galli, respectively. Herein, we present for the first time a molecular characterization of Cryptosporidium from feces of eared doves (Z. auriculata) and propose that these birds are a potential source of C. meleagridis infection in humans.

scholarly journals Cryptosporidium spp. in Domestic Dogs: the “Dog” Genotype

2000 ◽  
Vol 66 (5) ◽  
pp. 2220-2223 ◽  
Author(s):  
Una M. Morgan ◽  
Lihua Xiao ◽  
Paul Monis ◽  
Abbie Fall ◽  
Peter J. Irwin ◽  
...  
Keyword(s):  
18S Rrna ◽  
18S Rrna Gene ◽  
Domestic Dogs ◽  
Heat Shock Gene ◽  
Valid Species ◽  
Rrna Gene ◽  
Phylogenetic Characterization ◽  
Cryptosporidium Spp ◽  
Shock Gene

ABSTRACT Genetic and phylogenetic characterization ofCryptosporidium isolates at two loci (18S rRNA gene and heat shock gene) from both Australian and United States dogs demonstrated that dog-derived Cryptosporidium isolates had a distinct genotype which is conserved across geographic areas. Phylogenetic analysis provided support for the idea that the “dog” genotype is, in fact, a valid species.

scholarly journals Molecular Characterization of Cryptosporidium Isolates from Humans and Animals in Iran

2006 ◽  
Vol 73 (3) ◽  
pp. 1033-1035 ◽  
Author(s):  
Ahmad Reza Meamar ◽  
Karine Guyot ◽  
Gabriela Certad ◽  
Eduardo Dei-Cas ◽  
Mino Mohraz ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Predominant Species ◽  
Animal Hosts

ABSTRACT Isolates of Cryptosporidium spp. from human and animal hosts in Iran were characterized on the basis of both the 18S rRNA gene and the Laxer locus. Three Cryptosporidium species, C. hominis, C. parvum, and C. meleagridis, were recognized, and zoonotically transmitted C. parvum was the predominant species found in humans.

scholarly journals Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of Theileria haneyi in Egypt

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1414
Author(s):  
Bassma S. M. Elsawy ◽  
Ahmed M. Nassar ◽  
Heba F. Alzan ◽  
Raksha V. Bhoora ◽  
Sezayi Ozubek ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Genetic Characterization ◽  
Simultaneous Detection ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Pcr Targeting ◽  
First Time

Equine Piroplasmosis (EP) is an infectious disease caused by the hemoprotozoan parasites Theileria equi, Babesia caballi, and the recently identified species T. haneyi. Hereby, we used a multiplex PCR (mPCR) targeting the 18S rRNA gene of T. equi and B. caballi for the simultaneous detection of EP in Egyptian equids and examined the presence of T. haneyi infections in Egypt. Blood samples from 155 equids (79 horses and 76 donkeys) collected from different governorates of Egypt were examined by mPCR and PCR targeting T. hayeni. The mPCR method revealed a prevalence of T. equi of 20.3% in horses and of 13.1% in donkeys and a prevalence of B. caballi of 1.2% in horses. B. caballi was not detected in donkeys in the current study. The mPCR method also detected coinfections with both species (2.5% and 1.3% in horses and donkeys, respectively). Additionally, we report the presence of T. haneyi in Egypt for the first time in 53.1% of the horse and 38.1% of the donkey tested samples. Coinfection with T. haneyi and T. equi was found in 13.5% of the samples, while infection with the three EP species was found in 1.9% of the samples.

scholarly journals Prevalence and molecular characterization of Cryptosporidium spp. in Père David’s deer (Elaphurus davidianus) in Jiangsu, China

2020 ◽  
Vol 29 (2) ◽  
Author(s):  
Si-Yang Huang ◽  
Yi-Min Fan ◽  
Yi Yang ◽  
Yi-Jun Ren ◽  
Jing-Zhi Gong ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Small Subunit ◽  
Basic Knowledge ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Ssu Rrna Gene ◽  
Cryptosporidium Spp ◽  
Père David’S Deer ◽  
Rrna Gene Sequencing

Abstract Cryptosporidium is a zoonotic parasite that causes diarrhea in a broad range of animals, including deer. Little is known about the prevalence and genotype of Cryptosporidium spp. in Père David’s deer. In this study, 137 fecal samples from Père David’s deer were collected between July 2017 and August 2018 in the Dafeng Reserve and analyzed for Cryptosporidium spp. by nested-PCR based on the small subunit ribosomal RNA (SSU rRNA) gene, followed by sequence analyses to determine the species. The 60 kDa glycoprotein (gp60) gene was used to characterize Cryptosporidium spp. Among 137 samples, 2 (1.46%) were positive for Cryptosporidium spp. according to SSU rRNA gene sequencing results. Both samples belonged to the Cryptosporidium deer genotype, with two nucleotide deletions and one nucleotide substitution. The prevalence data and molecular characterization of this study provide basic knowledge for controlling and preventing Cryptosporidium infections in Père David’s deer in this area.

scholarly journals Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil

2014 ◽  
Vol 23 (3) ◽  
pp. 383-386 ◽  
Author(s):  
Gustavo Freire Figueira ◽  
Victor Henrique Silva de Oliveira ◽  
Alessandra Taroda ◽  
Amauri Alcindo Alfieri ◽  
Selwyn Arlington Headley
Keyword(s):  
Molecular Characterization ◽  
Gene Sequence ◽  
Domestic Animals ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sequence Analyses ◽  
Pcr Assay ◽  
Bovine Pancreas

This study investigated the occurrence of Eurytremaspp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.

scholarly journals First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR

2017 ◽  
Vol 7 (9) ◽  
pp. 548-552 ◽  
Author(s):  
Mohd Aiman Barudin ◽  
◽  
Muhammad Lokman Md Isa ◽  
Noratikah Othman ◽  
Afzan Mat Yusof ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Nested Pcr ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene

scholarly journals Distribution and molecular characterization of biosurfactant-producing bacteria

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Nassir Alyousif ◽  
YASIN Y.Y. AL LUAIBI ◽  
WIJDAN HUSSEIN
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Bacillus Licheniformis ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Emulsification Activity ◽  
Emulsification Index ◽  
First Time

Abstract. Alyousif NA, Luaibi YYYA, Hussein W. 2020. Distribution and molecular characterization of biosurfactant-producing bacteria. Biodiversitas 21: 4034-4040. Biosurfactants (BSs) are biological surface-active compounds produced by several microorganisms with many areas of application, as such become an important product in biotechnology and consequence to be used in industries. In recent years, many researchers pay attention to BSs producers' microorganisms. The present study was aimed to isolate, identify, and screening BS producing bacteria from six various sites in two different cities in Iraq. Four samples were collected from four sites in Basrah governorate and the rest two samples from Al-Garraf oilfield in Thi-Qar governorate. A total of 33 different bacterial isolates were obtained, 20 out of the 33 were found to be biosurfactants producing isolates that detected through the emulsification index (E24%), oil spreading test, and emulsification activity. The isolated bacterial strains were more identified by 16S rRNA gene sequencing. The results showed that the biosurfactants producing isolates belonged to genera Bacillus, Pseudomonas, Enterobacter, Staphylococcus, Acinetobacter, and Aerococcus. Bacillus jeotgali and Aerococcus viridans are reporting as biosurfactant producing bacteria for the first time and Bacillus jeotgali is isolated for first time from crude oil of oilfield reservoir in this study in world. Moreover, six bacterial isolates were identified as new strains and deposited at NCBI Genbank under accession numbers MT261834 (Bacillus subtilis strain IRQNWYA3), MT261835 (Bacillus licheniformis strain IRQNWYB4), MT261836 (Pseudomonas stutzeri strain IRQNWYF2), MT261837 (Pseudomonas zhaodongensis strain IRQNWYF3), MT261838 (Pseudomonas sp. IRQNWYF4) and MT261839 (Bacillus licheniformis strain IRQNWYF5). A2 isolate that was identified as Pseudomonas aeruginosa has shown the highest values of emulsification activity and emulsification index (1.678±0.050 absorbance at 540 nm and 56.6% respectively) that show efficient potential of biosurfactant production. Phylogenetic tree was also constructed in this study based on 16S rRNA gene sequences of biosurfactant-producing bacteria to evaluate their close relationship and evolution between them.

scholarly journals Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil

2020 ◽  
Vol 41 (5supl1) ◽  
pp. 2437-2444
Author(s):  
Thábata dos Anjos Pacheco ◽  
Felippe Danyel Cardoso Martins ◽  
Sayanne Luns Hatum de Almeida ◽  
Thiago Borges Fernandes Semedo ◽  
Michelle Igarashi Watanabe ◽  
...  
Keyword(s):  
18S Rrna ◽  
18S Rrna Gene ◽  
The State ◽  
Severe Illness ◽  
Rrna Gene ◽  
Mato Grosso ◽  
Fecal Samples ◽  
Cryptosporidium Spp ◽  
Gp60 Gene ◽  
Wide Range

Cryptosporidium spp. is a protozoan that infects a wide range of vertebrate hosts; it has been reported to be the cause of severe illness or death in livestock worldwide, which leads to decreased performance and production losses, especially in young animals. This study investigated the presence of Cryptosporidium in calves from beef farms in the state of Mato Grosso, midwestern Brazil. For this purpose, fecal samples from 237 animals aged ? 45 days, raised in 20 rural properties were subjected to DNA extraction and nested polymerase chain reaction (nPCR) targeting 18S ribosomal RNA (18S rRNA) gene followed by sequencing. Additionally, positive samples, previously identified as Cryptosporidium parvum by sequencing and phylogenetic analyses based on 18S rRNA gene, were subsequently analyzed focusing the amplification and sequencing using nPCR of a fragment of the 60 kDa glycoprotein (gp60) gene. Of the 237 fecal samples analyzed by PCR (18S rRNA), 50 (21.1%) fecal samples were positive for Cryptosporidium spp., while 14 (70%) of the 20 properties had at least one positive animal. The following Cryptosporidium species were detected: C. bovis, C. parvum, and C. ryanae. Thereafter, two potentially zoonotic subtypes (IIaA15G2R1 and IIaA16G3R1) of C. parvum were identified based on gp60 gene sequences. This study resulted in the detection of subtype IIaA16G3R1 for the first time in South America and showed a wide distribution of the protozoan in beef farms in the studied area of the State.

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