scholarly journals Molecular detection of vector-borne agents in cats in Southern Brazil

2019 ◽  
Vol 28 (4) ◽  
pp. 632-643
Author(s):  
Daniela Pedrassani ◽  
Juliano Biolchi ◽  
Luiz Ricardo Gonçalves ◽  
Natalia Serra Mendes ◽  
Diego Carlos de Souza Zanatto ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Leishmania Infantum ◽  
Molecular Detection ◽  
Ehrlichia Canis ◽  
Zoonotic Potential ◽  
Southern Brazil ◽  
Pcr Assay ◽  
Vector Borne ◽  
The University ◽  
Veterinary Hospital

Abstract This study used serological and molecular methods to investigate the occurrence of vector-borne pathogens (VBP) with zoonotic potential in cats neutered at the University Veterinary Hospital in Canoinhas, Santa Catarina. The combined PCR and serological results revealed that 17 (56.6%) cats were positive for one or more pathogens. The sampled cats had antibodies to Ehrlichia spp. (7/30), Anaplasma phagocytophilum (3/30) and Leishmania infantum (2/30). The PCR assay detected DNA closely related to Ehrlichia canis in 6/30 cats, Mycoplasma haemofelis in 2/30 cats, A. phagocytophilum and Cytauxzoon sp. in one cat each. While Bartonella clarridgeiae and B. henselae were detected in two cats each, and B. koehlerae was detected in one cat.

scholarly journals Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil

2018 ◽  
Vol 27 (4) ◽  
pp. 505-513 ◽  
Author(s):  
Anna Cláudia Baumel Mongruel ◽  
Priscila Ikeda ◽  
Keyla Carstens Marques de Sousa ◽  
Jyan Lucas Benevenute ◽  
Margarete Kimie Falbo ◽  
...  
Keyword(s):  
18S Rrna ◽  
Molecular Detection ◽  
Phylogenetic Analyses ◽  
Southern Brazil ◽  
Conventional Pcr ◽  
Pathogenic Potential ◽  
Vector Borne ◽  
Pcr Assays ◽  
Etiological Agents ◽  
Positive Results

Abstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood was collected from 75 domestic dogs presenting anemia or/and thrombocytopenia from Guarapuava, state of Paraná, Brazil. DNA samples were subjected to conventional PCR assays for Ehrlichia spp. (dsb), piroplasmids (18S rRNA) and Hepatozoon spp. (18S rRNA), followed by sequencing and phylogenetic analyses. Among the 75 dogs, one (1.33%) was positive for Hepatozoon sp. and six (8%) were positive for piroplasmids in 18S rRNA cPCR assays. None of the dogs showed positive results in Ehrlichia spp.-cPCR targeting dsb gene. The phylogenetic analyses revealed that three piroplasm sequences were clustered with Rangellia vitalii, while one sequence was grouped with B. vogeli. The only sequence obtained from Hepatozoon spp.-PCR protocol was pooled with H. canis. Therefore, there is urgent need for differential molecular diagnosis of the two piroplasm species cited as etiological agents in clinical cases of canine hemoparasitic diseases, given the higher pathogenic potential of R. vitalii than of B. vogeli.

Molecular detection of Anaplasma platys, Anaplasma phagocytophilum and Wolbachia sp. but not Ehrlichia canis in Croatian dogs

2017 ◽  
Vol 116 (11) ◽  
pp. 3019-3026 ◽  
Author(s):  
Doroteja Huber ◽  
Irena Reil ◽  
Sanja Duvnjak ◽  
Daria Jurković ◽  
Damir Lukačević ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Molecular Detection ◽  
Ehrlichia Canis ◽  
Anaplasma Platys

scholarly journals Hematological and histopathological changes in medular aplasia resulting from Ehrlichia canis infection in a Border collie dog

2021 ◽  
Vol 15 (4) ◽  
pp. 275-280
Author(s):  
Carolina Araújo Neves ◽  
Reiner Silveira de Moraes ◽  
Kaline Ogliari ◽  
Antônio Carlos Severino Neto ◽  
Dirceu Guilherme de Souza Ramos ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adipose Tissue ◽  
Rhipicephalus Sanguineus ◽  
Ehrlichia Canis ◽  
Major Constituent ◽  
Long Bones ◽  
Histopathological Changes ◽  
Canine Monocytic Ehrlichiosis ◽  
The University ◽  
Veterinary Hospital

The Canine Monocytic Ehrlichiosis (CME) is an infectious disease that commonly affects dogs of all breeds and ages. It is caused by the bacterium Ehrlichia canis and is transmitted by the tick Rhipicephalus sanguineus. The disease may pre-sent itself in the acute, subclinical, and chronic forms. The present study reports the case of a 2-year-old male Border Collie with advanced stage CME, attended at the Pet Clinic of the Veterinary Hospital of the University Federal de Jataí, which resul-ted in medullary aplasia. The diagnosis of marrow aplasia was based on the necroscopic and histopathological examinations. At necropsy, the diaphyses of the long bones were filled with diffuse, strongly whitish and pasty tissue, typical of the adipose tissue, also found in the femoral epiphyses. The histopathology showed unilocular adipose tissue as the major constituent of the bone marrow and rare islands of marrow cells. These findings were compatible with severe hypoplasia of the red bone mar-row and hyperplasia of the white bone marrow, affecting hematopoiesis, resulting in the laboratory alterations observed in the hematocrit, WBC, and plateletogram.

scholarly journals Seroprevalence Canine Survey For Selected Vector-Borne Pathogens of and Its Relationship With Poverty in Metropolitan Pereira, Colombia, 2020*

2021 ◽  
Author(s):  
D. Katterine Bonilla-Aldana ◽  
Erwin J. Gutiérrez-Grajales ◽  
J. Paola Martínez-Arboleda ◽  
María Angelica Reina-Mora ◽  
Adrián E. Trejos-Mendoza ◽  
...  
Keyword(s):  
Socioeconomic Status ◽  
Latin American ◽  
Anaplasma Phagocytophilum ◽  
Test System ◽  
Ehrlichia Canis ◽  
Vector Borne Diseases ◽  
Lower Socioeconomic ◽  
Level Ii ◽  
Vector Borne ◽  
Level I

Abstract Background Tick-borne diseases (TBD) and dirofilariasis are currently not under surveillance in most Latin American countries, and there is a significant lack of studies describing the current situation in most endemic areas, including Colombia. Seroprevalence studies are crucial for understanding the epidemiology of these vector-borne diseases. Methods A serosurvey for TBD and dirofilariasis amongst 100 dogs was carried out in the municipality of Pereira, located in the Coffee-Triangle region, Colombia. Samples were tested using a rapid assay test system (SNAP® 4Dx®); based on an enzyme immunoassay technique‚ screening for antibodies to Anaplasma phagocytophilum/platys (sensitivity 99.1%)‚ Borrelia burgdorferi s.l. (98.8%), and Ehrlichia canis/ewingii (96.2%) by using specific antigens, and checking for Dirofilaria immitis antigen based on specific antibodies (99.2%). Bivariate analyses were performed on Stata®14, significant p < 0.05. Results Global seroprevalence to the selected vector-borne pathogens was 74% (95%CI 65–83%). The highest seroprevalence was found for Ehrlichia canis/ewingii (74%), followed by Anaplasma phagocytophilum/platys (16%). Seropositivity for Borrelia spp. and Dirofilaria spp. was 0%. All Anaplasma spp. seropositive dogs showed co-detection of Ehrlichia spp. (16%). Seroprevalence was significantly higher among dogs from families of lower socioeconomic status / level (I, 86%), followed by level II (74%), and III (36%) (p = 0.001); no seropositive dogs were found in for levels IV and V (higher socioeconomic status). All dogs exhibiting anorexia (12%) were invariably seropositive (100%) (p = 0.029). Seroprevalence was higher amongst those showing mucocutaneous paleness (95%) compared to those without paleness (68%) (p = 0.013) (OR = 9.3; 95%CI 1.18–72.9). There was high variability in seroprevalence through the studied areas, ranging from 0% (La Libertad Park) up to Combia, Cesar Nader, Las Brisas and Saturno localities (100%) (p = 0.033). Conclusions Given the high seroprevalence obtained in an area with documented presence of ticks, there is a potential risk for zoonotic transmission to humans. Further seroprevalence studies in humans are needed to assess the prevalence of infections. Poverty is highly associated with these tick-borne pathogens in Pereira, as shown in the present study.

scholarly journals Seroprevalence Canine Survey for Selected Vector-Borne Pathogens of and Its Relationship with Poverty in Metropolitan Pereira, Colombia, 2020

2021 ◽  
Author(s):  
D. Katterine Bonilla-Aldana ◽  
Erwin J. Gutiérrez-Grajales ◽  
J. Paola Martínez-Arboleda ◽  
María Angelica Reina-Mora ◽  
Adrián E. Trejos-Mendoza ◽  
...  
Keyword(s):  
Socioeconomic Status ◽  
Latin American ◽  
Anaplasma Phagocytophilum ◽  
Test System ◽  
Ehrlichia Canis ◽  
Vector Borne Diseases ◽  
Lower Socioeconomic ◽  
Level Ii ◽  
Vector Borne ◽  
Level I

Abstract Background: Tick-borne diseases (TBD) and dirofilariasis are currently not under surveillance in most Latin American countries, and there is a significant lack of studies describing the current situation in most endemic areas, including Colombia. Seroprevalence studies are crucial for understanding the epidemiology of these vector-borne diseases.Methods: A serosurvey for TBD and dirofilariasis amongst 100 dogs was carried out in the municipality of Pereira, located in the Coffee-Triangle region, Colombia. Samples were tested using a rapid assay test system (SNAP® 4Dx®); based on an enzyme immunoassay technique‚ screening for antibodies to Anaplasma phagocytophilum/platys (sensitivity 99.1%)‚ Borrelia burgdorferi s.l. (98.8%), and Ehrlichia canis/ewingii (96.2%) by using specific antigens, and checking for Dirofilaria immitis antigen based on specific antibodies (99.2%). Bivariate analyses were performed on Stata®14, significant p<0.05. Results: Global seroprevalence to the selected vector-borne pathogens was 74% (95%CI 65-83%). The highest seroprevalence was found for Ehrlichia canis/ewingii (74%), followed by Anaplasma phagocytophilum/platys (16%). Seropositivity for Borrelia spp. and Dirofilaria spp. was 0%. All Anaplasma spp. seropositive dogs showed co-detection of Ehrlichia spp. (16%). Seroprevalence was significantly higher among dogs from families of lower socioeconomic status / level (I, 86%), followed by level II (74%), and III (36%) (p=0.001); no seropositive dogs were found in for levels IV and V (higher socioeconomic status). All dogs exhibiting anorexia (12%) were invariably seropositive (100%) (p=0.029). Seroprevalence was higher amongst those showing mucocutaneous paleness (95%) compared to those without paleness (68%) (p=0.013) (OR=9.3; 95%CI 1.18-72.9). There was high variability in seroprevalence through the studied areas, ranging from 0% (La Libertad Park) up to Combia, Cesar Nader, Las Brisas and Saturno localities (100%) (p=0.033). Conclusions: Given the high seroprevalence obtained in an area with documented presence of ticks, there is a potential risk for zoonotic transmission to humans. Further seroprevalence studies in humans are needed to assess the prevalence of infections. Poverty is highly associated with these tick-borne pathogens in Pereira, as shown in the present study.

Molecular detection of Dirofilaria immitis, Hepatozoon canis, Babesia spp., Anaplasma platys and Ehrlichia canis in dogs on Costa Rica

2014 ◽  
Vol 60 (1) ◽  
Author(s):  
Lanjing Wei ◽  
Patrick Kelly ◽  
Kate Ackerson ◽  
Heba S. El-Mahallawy ◽  
Bernhard Kaltenboeck ◽  
...  
Keyword(s):  
Costa Rica ◽  
Dirofilaria Immitis ◽  
Molecular Detection ◽  
Ehrlichia Canis ◽  
Hepatozoon Canis ◽  
Anaplasma Platys ◽  
Vector Borne Diseases ◽  
Tropical Areas ◽  
Vector Borne ◽  
Babesia Spp

AbstractAlthough vector-borne diseases are important causes of morbidity and mortality in dogs in tropical areas, there is little information on these conditions in Costa Rica. In PCRs of blood from dogs in Costa Rica, we did not detect DNAs of Rickettsia (R.) felis and Coxiella (C.) burnetii but we did find evidence of infection with Dirofilaria (D.) immitis (9/40, 22.5%), Hepatozoon (H.) canis (15/40, 37.5%), Babesia spp. (10/40, 25%; 2 with B. gibsoni and 8 with B. vogeli), Anaplasma (A.) platys (3/40, 7.5%) and Ehrlichia (E.) canis (20/40, 50%). Nine dogs (22.5%) were free of any vector-borne pathogens while 14 (35%) were infected with a single pathogen, 11 (27.5%) with two, 4 (10%) with three, 1 (2.5%) with four, and 1 (2.5%) with five pathogens. Dogs in Costa Rica are commonly infected with vector-borne agents

scholarly journals Co-infection with arthropod-borne pathogens in domestic cats

2017 ◽  
Vol 26 (4) ◽  
pp. 525-531 ◽  
Author(s):  
Marcos Rogério André ◽  
Kilder Dantas Filgueira ◽  
Ana Cláudia Calchi ◽  
Keyla Carstens Marques de Sousa ◽  
Luiz Ricardo Gonçalves ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Clinical Signs ◽  
Ehrlichia Canis ◽  
Domestic Cats ◽  
Blood Samples ◽  
Cytauxzoon Felis ◽  
Blood Smears ◽  
Vector Borne ◽  
Biochemical Abnormalities

Abstract The role of several feline vector-borne pathogens (FVBP) as a cause of disease in cats has not been clearly determined. In fact, with the exception of Bartonella spp. and hemoplasmas, FVBP in cats has not been clearly determined in Brazil yet. The present study aimed at identifying, by using molecular methods, the presence of FVBP in three cats showing non-specific clinical signs and inclusions suggestive of hemoparasites in blood smears. Cytauxzoon felis, ‘Candidatus Mycoplasma haemominutum’, Ehrlichia sp. closely related to Ehrlichia canis, and Anaplasma sp. closely related to Anaplasma phagocytophilum were detected in blood samples from two out of three sampled cats. Both cats positive for multiple FVBP did not show hematological and biochemical abnormalities. The present work emphasizes the need for molecular confirmation of co-infection by multiple FVBP in cats presenting non-specific clinical signs and inclusions resembling hemoparasites in blood smears.

Human Sporotrichosis Outbreak caused by Sporothrix brasiliensis in a Veterinary Hospital in Southern Brazil

2021 ◽  
pp. 101163
Author(s):  
José Raphael Batista Xavier ◽  
Stefanie Bressan Waller ◽  
Luíza da Gama Osório ◽  
Patrícia Silva Vives ◽  
Ana Paula Neuschrank Albano ◽  
...  
Keyword(s):  
Southern Brazil ◽  
Sporothrix Brasiliensis ◽  
Veterinary Hospital
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