scholarly journals Hematological and histopathological changes in medular aplasia resulting from Ehrlichia canis infection in a Border collie dog

2021 ◽  
Vol 15 (4) ◽  
pp. 275-280
Author(s):  
Carolina Araújo Neves ◽  
Reiner Silveira de Moraes ◽  
Kaline Ogliari ◽  
Antônio Carlos Severino Neto ◽  
Dirceu Guilherme de Souza Ramos ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adipose Tissue ◽  
Rhipicephalus Sanguineus ◽  
Ehrlichia Canis ◽  
Major Constituent ◽  
Long Bones ◽  
Histopathological Changes ◽  
Canine Monocytic Ehrlichiosis ◽  
The University ◽  
Veterinary Hospital

The Canine Monocytic Ehrlichiosis (CME) is an infectious disease that commonly affects dogs of all breeds and ages. It is caused by the bacterium Ehrlichia canis and is transmitted by the tick Rhipicephalus sanguineus. The disease may pre-sent itself in the acute, subclinical, and chronic forms. The present study reports the case of a 2-year-old male Border Collie with advanced stage CME, attended at the Pet Clinic of the Veterinary Hospital of the University Federal de Jataí, which resul-ted in medullary aplasia. The diagnosis of marrow aplasia was based on the necroscopic and histopathological examinations. At necropsy, the diaphyses of the long bones were filled with diffuse, strongly whitish and pasty tissue, typical of the adipose tissue, also found in the femoral epiphyses. The histopathology showed unilocular adipose tissue as the major constituent of the bone marrow and rare islands of marrow cells. These findings were compatible with severe hypoplasia of the red bone mar-row and hyperplasia of the white bone marrow, affecting hematopoiesis, resulting in the laboratory alterations observed in the hematocrit, WBC, and plateletogram.

Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues From Rhipicephalus sanguineus (Acari: Ixodidae) Ticks in Areas Endemic for Canine Monocytic Ehrlichiosis in Brazil

2018 ◽  
Vol 56 (3) ◽  
pp. 828-831
Author(s):  
Bruno César Miranda Oliveira ◽  
Elis Domingos Ferrari ◽  
Milena Araúz Viol ◽  
Marcos Rogério André ◽  
Rosangela Zacarias Machado ◽  
...  
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Ehrlichia Canis ◽  
Canine Monocytic Ehrlichiosis

Amplification of Ehrlichial DNA from Dogs 34 Months after Infection with Ehrlichia canis

1998 ◽  
Vol 36 (1) ◽  
pp. 73-76 ◽  
Author(s):  
Shimon Harrus ◽  
Trevor Waner ◽  
Itzhak Aizenberg ◽  
Janet E. Foley ◽  
Amy M. Poland ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Carrier State ◽  
Ehrlichia Canis ◽  
Beagle Dogs ◽  
Three Samples ◽  
Canine Monocytic Ehrlichiosis ◽  
Antibody Titers ◽  
Healthy Dogs ◽  
First Time ◽  
Indirect Immunofluorescent

In order to determine whether dogs in the subclinical phase of canine monocytic ehrlichiosis (CME) are carriers of Ehrlichia canis and to determine the significance of persistent indirect immunofluorescent anti-E. canis antibody titers during this phase, PCR was performed with blood, bone marrow, and splenic aspirates collected 34 months postinoculation from six clinically healthy beagle dogs experimentally infected with E. canis. At least one of the three samples (spleen, bone marrow, and blood) from four of the six dogs was PCR positive. The spleens of all four of these dogs were PCR positive, and the bone marrow and blood of two of the four dogs were PCR positive. Indirect immunofluorescent-antibody titers increased progressively during the first 5 months postinfection, remained high for an additional period of more than 11 months, and declined thereafter, suggesting that the dogs were recovering from the disease. Five of the dogs remained seropositive 34 months postinfection. The data obtained in this study demonstrate for the first time that clinically healthy dogs in the subclinical phase of CME are carriers of the rickettsia. It was shown that dogs can harbor E. canisfor years without developing the chronic clinical disease and that dogs can eliminate the parasite and recover from CME without medical treatment. Our findings suggest that the spleen is the organ most likely to harbor E. canis parasites during the subclinical phase and the last organ to accommodate the parasite before elimination. It was concluded that PCR of DNA extracted from splenic aspirates is a reliable method for determining the carrier state of CME.

scholarly journals Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis

2018 ◽  
Vol 39 (3) ◽  
pp. 1049
Author(s):  
Jéssica Fontes Veloso ◽  
Leonardo Sauer ◽  
Arianne Pontes Oriá ◽  
Deusdete Conceição Gomes Junior ◽  
Ana Cláudia Santos Raposo ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Infectious Agent ◽  
Posterior Uveitis ◽  
Ehrlichia Canis ◽  
State University ◽  
Nested Polymerase Chain Reaction ◽  
Canine Monocytic Ehrlichiosis ◽  
Polymerase Chain ◽  
Clinical Changes ◽  
Veterinary Hospital

Canine monocytic ehrlichiosis (CME) is an infectious disease caused by a gram-negative bacterium Ehrlichia canis that has a high global prevalence that leads to high rates of morbidity and mortality in dogs. Among the clinical changes, ophthalmic diseases can lead to permanent blindness and it can be an important clinical sign. The objective of this study was to perform nested polymerase chain reaction (PCR) to diagnose E. canis infection in dogs with bilateral uveitis from the Veterinary Hospital of the Santa Cruz State University. Blood samples were collected and DNA for the molecular diagnosis was extracted from 66 adult dogs of both genders and mixed breeds diagnosed with bilateral uveitis. Thirty-five (53%) dogs showed positive results and presented with iridocyclitis, posterior uveitis, panuveitis, or uveitis with secondary glaucoma. This study demonstrates that nested PCR is an important tool for the differential diagnosis of dogs with bilateral uveitis, as it provides evidence of the infectious agent in the animal.

scholarly journals Efficacy of a Doxycycline Treatment Regimen Initiated during Three Different Phases of Experimental Ehrlichiosis

2010 ◽  
Vol 54 (12) ◽  
pp. 5012-5020 ◽  
Author(s):  
Jennifer C. McClure ◽  
Michelle L. Crothers ◽  
John J. Schaefer ◽  
Patrick D. Stanley ◽  
Glen R. Needham ◽  
...  
Keyword(s):  
Antibiotic Therapy ◽  
Treatment Regimen ◽  
Rhipicephalus Sanguineus ◽  
Ehrlichia Canis ◽  
Clinical Parameters ◽  
Blood Samples ◽  
Persistently Infected ◽  
Doxycycline Treatment ◽  
Canine Monocytic Ehrlichiosis ◽  
Three Phases

ABSTRACT Doxycycline is the treatment of choice for canine monocytic ehrlichiosis (CME), a well-characterized disease and valuable model for tick-borne zoonoses. Conflicting reports of clearance of Ehrlichia canis after treatment with doxycycline suggested that the disease phase during which treatment is initiated influences outcomes of these treatments. The purpose of this study was to evaluate the efficacy of a 28-day doxycycline regimen for clearance of experimental E. canis infections from dogs treated during three phases of the disease. Ten dogs were inoculated with blood from E. canis carriers and treated with doxycycline during acute, subclinical, or chronic phases of CME. Daily rectal temperatures and semiweekly blood samples were monitored from each dog, and Rhipicephalus sanguineus ticks were acquisition fed on each dog for xenodiagnosis. Blood collected from dogs treated during acute or subclinical CME became PCR negative for E. canis as clinical parameters improved, but blood samples collected from dogs treated during chronic CME remained intermittently PCR positive. R. sanguineus ticks fed on dogs after doxycycline treatments became PCR positive for E. canis, regardless of when treatment was initiated. However, fewer ticks became PCR positive after feeding on two persistently infected dogs treated with doxycycline followed by rifampin, suggesting that antibiotic therapy can reduce tick acquisition of E. canis.

scholarly journals SEROPREVALENCE OF CANINE MONOCYTIC EHRLICHIOSIS IN HUNTING DOGS IN THE AUTONOMOUS PROVINCE OF VOJVODINA, SERBIA

2015 ◽  
Vol 7 (1) ◽  
pp. 11-18
Author(s):  
Aleksandar Potkonjak ◽  
Sara Savić ◽  
Ljubica Spasojević Kosić ◽  
Vuk Vračar ◽  
Radmila Kudus ◽  
...  
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Ehrlichia Canis ◽  
Transmitted Infection ◽  
Hunting Dogs ◽  
Mediterranean Countries ◽  
Bacterial Vector ◽  
Canine Monocytic Ehrlichiosis ◽  
Autonomous Province ◽  
Epidemiological Characteristics ◽  
Continental Climate

Canine monocytic ehrlichiosis is a bacterial, vector-transmitted infection caused by Ehrlichia canis. Th e pathogen is mainly transmitted by ticks Rhipicephalus sanguineus. The disease highest distributionis most widely distributed in tropical and subtropical countries but it is also reported in Mediterranean countries of Europe (Spain, France, Italy, Turkey). Temperate continental climate and presence of these tick species are responsible for the maintenance and spreading of canine monocytic ehrlichiosis within the dog population in our region as well. Since hunting dogs are more exposed to tick bites then pet dogs, ed thisour study was conducted with the aims of determining the seroprevalence and basic epidemiological characteristics of monocytic ehrlichiosis infection in the population of hunting dogs, and comparing the obtained results with the results of other authors. This research involved 58 hunting dogs from the region of Autonomous Province of Vojvodina. All dogs were clinically examined and their basic epidemiological characteristics were recorded. Then, blood samples were collected in order to determine the presence of specifi c G class antibodies against Ehrlichia canis antigens. An indirect immunofl uorescence test manufactured by VMRD, U.S.A., was used. In this study, the seroprevalence of monocytic ehrlichiosis in a population of examined hunting dogs from the region of Vojvodina was 13.79%. Th is rate is similar to the seroprevalence of monocytic ehrlichiosis in the general population of dogs in Vojvodina.

scholarly journals Molecular detection of vector-borne agents in cats in Southern Brazil

2019 ◽  
Vol 28 (4) ◽  
pp. 632-643
Author(s):  
Daniela Pedrassani ◽  
Juliano Biolchi ◽  
Luiz Ricardo Gonçalves ◽  
Natalia Serra Mendes ◽  
Diego Carlos de Souza Zanatto ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Leishmania Infantum ◽  
Molecular Detection ◽  
Ehrlichia Canis ◽  
Zoonotic Potential ◽  
Southern Brazil ◽  
Pcr Assay ◽  
Vector Borne ◽  
The University ◽  
Veterinary Hospital

Abstract This study used serological and molecular methods to investigate the occurrence of vector-borne pathogens (VBP) with zoonotic potential in cats neutered at the University Veterinary Hospital in Canoinhas, Santa Catarina. The combined PCR and serological results revealed that 17 (56.6%) cats were positive for one or more pathogens. The sampled cats had antibodies to Ehrlichia spp. (7/30), Anaplasma phagocytophilum (3/30) and Leishmania infantum (2/30). The PCR assay detected DNA closely related to Ehrlichia canis in 6/30 cats, Mycoplasma haemofelis in 2/30 cats, A. phagocytophilum and Cytauxzoon sp. in one cat each. While Bartonella clarridgeiae and B. henselae were detected in two cats each, and B. koehlerae was detected in one cat.

scholarly journals Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia

2021 ◽  
Vol 71 (2) ◽  
pp. 230-238
Author(s):  
Elena Atanaskova Petrov ◽  
Irena Celeska ◽  
Zagorka Popova ◽  
Kiril Krstevski ◽  
Igor Djadjovski
Keyword(s):  
Molecular Detection ◽  
Clinical Symptoms ◽  
Rhipicephalus Sanguineus ◽  
Ehrlichia Canis ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Positive Serology ◽  
Western Asia ◽  
Canine Monocytic Ehrlichiosis ◽  
The City

Abstract Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused by an obligate intracellular bacterium, Ehrlichia canis. The main vector, a brown-dog tick, Rhipicephalus sanguineus, is widely distributed, especially in areas with tropic, subtropic, or Mediterranean climates (Central and South America, Eastern and Western Asia, Africa, Australia and Southern Europe). The study performed in 2012, by Stefanovska et al., determined a seroprevalence of 18.7% of E. canis among the Macedonian dog population. Up to date, the presence of E. canis, using molecular diagnostic methods, has not been investigated in Macedonia. Therefore, this study aimed to confirm the presence of E. canis, in the pet-dog population on the territory of the city of Skopje, North Macedonia, using a highly sensitive multiplex Real-Time PCR method (qPCR). Whole blood samples from 80 dogs of different breeds and ages, with clinical symptoms of CME and positive serology result for the presence of antibodies against E.canis, were collected for analyses. Out of 80 dogs, 36 (45%) were found as positive. The present work reports the first molecular detection of E. canis in pet dogs on the territory of the city of Skopje, Macedonia.

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