Memory retrieval improvement by Heteropterys aphrodisiaca in aging rats

Few data exists about the pharmacological properties of Heteropterys aphrodisiaca O. Mach. (Malpighiaceae), which is native to the scrubland regions of Brazil. The present study investigated the effects of oral treatment with H. aphrodisiaca extract (BST0298) on the learning and memory of young (3-6 months) and aged (21-23 months) rats, and compared the in vitro antioxidant activity of three lots collected in different years. An improvement in the number of sessions to learn the task was observed in the left/right discrimination test in aged rats treated for 45 days with 25 mg/kg (7.0 ± 0.5; p=0.005) or 50 mg/kg (7.6 ± 0.6; p=0.012) compared with control old rats (11.0 ± 1.6). On the other hand, pre-treatment did not improve the performance of scopolamine-treated mice in the passive avoidance test. The in vitro malondialdehyde test showed that all three different extracts presented similar antioxidant activity. The flavonoids astilbin, isoastilbin and neoastilbin were isolated from the extract and may contribute to the biological activity. These results suggest that repeated treatment with H. aphrodisiaca improves learning and memory, probably by a non-muscarinic mechanism.

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Green coffee extract attenuates Parkinson’s-related behaviors in animal models

10.21203/rs.3.rs-129362/v2 ◽

2021 ◽

Author(s):

Graziella Molska ◽

Lyvia Izaura Paula-Freire ◽

Marna Sakalem ◽

Daniele Köhn ◽

Giuseppina Negri ◽

...

Keyword(s):

Antioxidant Activity ◽

Medial Forebrain Bundle ◽

Oral Treatment ◽

Coffee Consumption ◽

Epidemiological Studies ◽

Experimental Models ◽

Inverse Association ◽

Green Coffee ◽

In Vitro Antioxidant Activity

Abstract Epidemiological studies have shown an inverse association between coffee consumption and the development of Parkinson’s disease (PD). The present investigation aimed to evaluate the effects of caffeine and green (non-roasted) coffee extract in experimental models of PD. The effects of the oral treatment with green coffee extracts (CE, Coffea arabica 100 or 400 mg/kg) and caffeine (31.2 mg/kg) were evaluated on catalepsy induced by haloperidol in mice, and unilateral 6-OHDA lesion of medial forebrain bundle (MFB) or striatum in rats. Also, the in vitro antioxidant activity and the monoamine levels in the striatum were investigated. CE presented a mild antioxidant activity in vitro and its administration decreased the catalepsy index. CE at the dose of 400 mg/kg induced ipsilateral rotations 14 days after the lesion; however, chronic 30-day CE and caffeine treatments did not interfere with the animals’ rotation after apomorphine or methamphetamine challenges in animals with MFB lesion, nor on monoamines levels. Furthermore, CE and caffeine were effective in inhibiting the asymmetry between ipsilateral and contralateral rotations induced by methamphetamine and apomorphine in animals with lesion in the striatum but did not avoid the monoamines depletion. These results suggest a pro-dopaminergic action of CE, although its mechanism remains unclear.

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Green Coffee Extract Blockades Haloperidol-Induced Catalepsy and Contralateral Rotations Induced by Apomorphine After Unilateral 6-OHDA Lesion on Striatum

10.21203/rs.3.rs-129362/v1 ◽

2020 ◽

Author(s):

Graziella Molska ◽

Lyvia Izaura Paula-Freire ◽

Marna Sakalem ◽

Daniele Köhn ◽

Giuseppina Negri ◽

...

Keyword(s):

Antioxidant Activity ◽

Medial Forebrain Bundle ◽

Oral Treatment ◽

Coffee Consumption ◽

Epidemiological Studies ◽

Experimental Models ◽

Inverse Association ◽

Green Coffee ◽

In Vitro Antioxidant Activity

Abstract Epidemiological studies have shown an inverse association between coffee consumption and the development of Parkinson’s disease (PD). The present investigation aimed to evaluate the effects of caffeine and green (non-roasted) coffee extract in experimental models of PD. The effects of the oral treatment with green coffee extracts (CE, Coffea arabica 100 or 400 mg/kg) and caffeine (31.2 mg/kg) were evaluated on the catalepsy induced by haloperidol in mice and unilateral 6-OHDA lesion of medial forebrain bundle (MFB) or striatum in rats. Also, the in vitro antioxidant activity and the monoamine levels in the striatum were investigated. CE presented a mild antioxidant activity and administration decreased the catalepsy index. CE at the dose of 400 mg/kg induced ipsilateral rotations 14 days after the lesion. However, the 30-day CE and caffeine treatments did not interfere with the animals’ rotation after apomorphine or methamphetamine challenges in animals with MFB lesion, nor on monoamines levels. Furthermore, CE and caffeine were effective in inhibiting the asymmetry between ipsilateral and contralateral rotations induced by methamphetamine and apomorphine in animals with lesion in the striatum but did not avoid the monoamines depletion. These results suggest a pro-dopaminergic action of CE, although its mechanism remains unclear.

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