Exemplar Abstract for Histophilus somni Angen et al. 2003.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Histophilus Somni

The problem of bovine histophilosis and Histophilus somni DNA detection by real-time PCR

2020 ◽  
Vol 23 (11) ◽  
pp. 28-33
Author(s):  
S.P. Yatsentyuk ◽  
◽  
D.A. Rudnyayev ◽  
Yu.I. Pobolelova ◽  
M.S. Krasnikova ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Detection ◽  
Histophilus Somni

Molecular Detection of Bacterial Pathogens Directly from the Nasal Swabs and Lung Tissue of Sheep and Goats

2019 ◽  
Vol 15 (02) ◽  
pp. 22-25
Author(s):  
Sunaina Thakur ◽  
Subhash Verma ◽  
Prasenjit Dhar ◽  
Mandeep Sharma
Keyword(s):  
Pasteurella Multocida ◽  
Direct Detection ◽  
Bacterial Species ◽  
Economic Losses ◽  
Isolation And Identification ◽  
Sheep And Goats ◽  
Histophilus Somni ◽  
Nasal Swabs ◽  
Mycoplasma Spp

Respiratory infections of sheep and goats cause heavy morbidity and mortality, leading to huge economic losses. Conventional methods of diagnosis that include isolation and identification of incriminating microbes are time-consuming and fraught with logistic challenges. Direct detection of incriminating microbes using molecular tools is gaining popularity in clinical, microbiological settings. In this study, a total of 50 samples (44 nasal swabs and 6 lung tissues) from sheep and goats were screened for the detection of different bacterial species by in vitro amplification of genus or species-specific genes. Histophilus somni was detected in 2% goat samples, Trueperella pyogenes in 20% goat nasal swabs, whereas 22% goat nasal swab samples were found positive for Mycoplasma spp. None of the samples from sheep was detected positive for H. somni, T. pyogenes, Mycoplasma spp. Similarly, all samples, irrespective, whether from sheep or goats, showed negative results for Pasteurella multocida, Mannheimia haemolytica, and Corynebacterium pseudotuberculosis.

scholarly journals Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

2014 ◽  
Vol 12 (1) ◽  
pp. 11
Author(s):  
Francisca Elda Ferreira Dias ◽  
Cáris Maroni Nunes ◽  
Tânia Vasconcelos Cavalcante ◽  
Juliano Franco De Souza ◽  
Silvia Minharro Barbosa ◽  
...  
Keyword(s):  
Histophilus Somni ◽  
Haemophilus Somnus

O presente estudo avaliou o uso da PCR para a detecção do Histophilus somni no sêmen bovino. Amostras de sêmen foram contaminadas experimentalmente com H. somni diluída em escalas de 107 a 101 bactérias/mL, submetidas à extração de DNA pelo método de fenol/clorofórmio e amplificadas pela PCR. Os produtos da amplificação do DNA foram analisados por eletroforese em gel de acrilamida 8%. Por meio de oligonucleotídeos iniciadores obteve-se a amplificação de um fragmento de 400 pares de bases a partir do DNA da bactéria. Conseguiu-se amplificação positiva até na diluição de 101 bactérias/mL. A PCR mostrou-se eficiente na detecção de H. somni. O resultado disponibiliza conhecimento relevante para o diagnóstico de H. somni, justificando a necessidade do diagnóstico dessa bactéria em reprodutores, especialmente em amostras de sêmen, que deveriam estar livres de qualquer contaminação. A PCR mostrou-se como uma valiosa ferramenta no controle da qualidade do sêmen produzido em centrais de inseminação artificial.

scholarly journals Evaluation of Antibody Response to Inactivated Histophilus somni Vaccine in Young Japanese Black Calves

2019 ◽  
Vol 72 (8) ◽  
pp. 477-480
Author(s):  
Konosuke OTOMARU ◽  
Katsuhisa NAGAI ◽  
Koji HYAKUTAKE ◽  
Shuichi KUBOTA ◽  
Takahiro KANESHIGE ◽  
...  
Keyword(s):  
Antibody Response ◽  
Histophilus Somni

scholarly journals Estimating Clinically Relevant Cut-Off Values for a High-Throughput Quantitative Real-Time PCR Detecting Bacterial Respiratory Pathogens in Cattle

2021 ◽  
Vol 8 ◽  
Author(s):  
Alicia F. Klompmaker ◽  
Maria Brydensholt ◽  
Anne Marie Michelsen ◽  
Matthew J. Denwood ◽  
Carsten T. Kirkeby ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Pasteurella Multocida ◽  
Age Groups ◽  
Therapeutic Interventions ◽  
Respiratory Pathogens ◽  
Logistic Regression Models ◽  
Histophilus Somni ◽  
Nasal Swabs

Bovine respiratory disease (BRD) results from interactions between pathogens, environmental stressors, and host factors. Obtaining a diagnosis of the causal pathogens is challenging but the use of high-throughput real-time PCR (rtPCR) may help target preventive and therapeutic interventions. The aim of this study was to improve the interpretation of rtPCR results by analysing their associations with clinical observations. The objective was to develop and illustrate a field-data driven statistical method to guide the selection of relevant quantification cycle cut-off values for pathogens associated with BRD for the high-throughput rtPCR system “Fluidigm BioMark HD” based on nasal swabs from calves. We used data from 36 herds enrolled in a Danish field study where 340 calves within pre-determined age-groups were subject to clinical examination and nasal swabs up to four times. The samples were analysed with the rtPCR system. Each of the 1,025 observation units were classified as sick with BRD or healthy, based on clinical scores. The optimal rtPCR results to predict BRD were investigated for Pasteurella multocida, Mycoplasma bovis, Histophilus somni, Mannheimia haemolytica, and Trueperella pyogenes by interpreting scatterplots and results of mixed effects logistic regression models. The clinically relevant rtPCR cut-off suggested for P. multocida and M. bovis was ≤ 21.3. For H. somni it was ≤ 17.4, while no cut-off could be determined for M. haemolytica and T. pyogenes. The demonstrated approach can provide objective support in the choice of clinically relevant cut-offs. However, for robust performance of the regression model sufficient amounts of suitable data are required.

scholarly journals Diagnostic Exercise: Myocarditis Due to Histophilus somni in Feedlot and Backgrounded Cattle

2009 ◽  
Vol 46 (5) ◽  
pp. 1015-1017 ◽  
Author(s):  
D. O'Toole ◽  
T. Allen ◽  
R. Hunter ◽  
L. B. Corbeil
Keyword(s):  
Differential Diagnosis ◽  
Bacterial Culture ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
Left Ventricular Myocardium ◽  
Papillary Muscles ◽  
Gram Negative ◽  
Histophilus Somni ◽  
Acute Necrotizing ◽  
Gross Lesions

A backgrounding operation for calves in Wyoming identified a disease syndrome presenting as lethargy, fever, and death between November and January each year. An unfixed heart was submitted for examination, along with samples of lung. There was focal red discoloration in papillary muscle of the left ventricular myocardium. Histologically, the lesion corresponded to acute necrotizing myocarditis with myriad intravascular and intralesional Gram-negative coccobacilli. Histophilus somni was detected by bacterial culture and immunohistochemistry. Focal myocarditis due to H. somni occurs in fall-placed cattle in western provinces and states of North America, and it can be an appreciable source of death loss. Gross lesions are readily detected in affected hearts. The presence of such changes in papillary muscles of left ventricular myocardium in feedlot or backgrounded cattle should prompt a differential diagnosis of H. somni myocarditis.

Detección de Pasteurella multocida, Mannhemia haemolytica, Histophilus somni y Mycoplasma bovis en pulmón de bovinos

2021 ◽  
Vol 12 (3) ◽  
pp. 710-720
Author(s):  
Seyda Cengiz ◽  
M. Cemal Adıgüzel ◽  
Gökçen Dinç
Keyword(s):  
Immune System ◽  
Pasteurella Multocida ◽  
Positive Sample ◽  
Mycoplasma Bovis ◽  
Opportunistic Pathogens ◽  
Specific Primers ◽  
Histophilus Somni ◽  
Healthy Cattle ◽  
Positive Results ◽  
Molecular Evaluation

In this study, it was aimed to determine of P. multocida, M. haemolytica, H. somni and M. bovis in macroscopically healthy cattle lungs by PCR. The study was carried out on 82 macroscopically healthy cattle lung. DNA extraction was performed to the lung samples. PCR was then performed using all specific primers. By molecular evaluation, positive results  were achieved for  P. multocida,  M. haemolytica,  H. somni and  M. bovis in 4 (4.8 %), 4 (4.8 %), 6 (7.3 %) and 3 (3.6 %) of the samples, respectively. Mix infections were detected in five samples. Of the samples, two were positive for both P. multocida and M. haemolytica, two were positive for both M. haemolytica and H. somni and one was positive for both P. multocida and H. somni. However, a positive sample, which carried all of pathogens, was not detected. In conclusion, P. multocida, M. haemolytica, H. somni and M. bovis are the important opportunistic pathogens of respiratory tract in cattle and these pathogens have a major role during infections. But multifactorial nature of bovine respiratory disease and immune system affected the formation of the disease. Hence, firstly cattle’s immunity should be strengthened and other conditions should be kept under control.

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