Exemplar Abstract for Acidianus manzaensis Yoshida et al. 2006.

The sulfur activation by extracellular proteins is considered as the crucial stage during biooxidation of elemental sulfur (S0). In order to study genes encoding sulfur-activation related extracellular proteins of extremely thermophilic Acidianus manzaensis, the extracellular proteins with higher abundance for the strain grown on S0 allotropes than that on Fe2+ were first screened by two-dimensional gel electrophoresis, and then identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Nine genes amplified with PCR were satisfactory according to their agarose gel electrophoresis. The differential expression of these nine genes when the strain grown on S0 allotropes and Fe2+ were analyzed with real-time quantitative PCR (RT-qPCR). Results showed that seven of them were higher expressed when the strain grown on S0 allotropes than on Fe2+, indicating they may be related with sulfur activation by A. manzaensis.

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Evolution of Compositions and Contents of Capsule and Slime EPSs for Adaptation to and Action on Energy Substrates and Heavy Metals by Typical Bioleaching Microorganisms

Solid State Phenomena ◽

10.4028/www.scientific.net/ssp.262.466 ◽

2017 ◽

Vol 262 ◽

pp. 466-470 ◽

Cited By ~ 2

Author(s):

Zhen Yuan Nie ◽

Hong Chang Liu ◽

Jin Lan Xia ◽

Huan Liu ◽

Yun Lu Cui ◽

...

Keyword(s):

Heavy Metals ◽

Heavy Metal Ions ◽

Elemental Sulfur ◽

Uronic Acids ◽

Energy Substrates ◽

Inorganic Matter ◽

Acidianus Manzaensis ◽

First Time ◽

Extracellular Polymer ◽

Mineral Interaction

Adaption to the energy substrates and heavy metals by bioleaching micoorganisms is the prerequisite for efficient microbe-mineral interaction in bioleaching process. It is known extracellular polymer substances (EPSs) take important role in mediating the adaption to and action on energy substrates and heavy metals. This report presents the evolution of compositions and contents of the major components of EPSs of the typical bioleaching microorganisms (Acidithiobacillus ferrooxidans, Leptospirillum ferriphilum, Sulfobacillus thermosulfidooxidans, and Acidianus manzaensis,) exposed to different energy substrates and heavy metal ions. These strains were acclimated firstly to Fe2+ substrate, and then on the substrates elemental sulfur (S0), pyrite and chalcopyrite, respectively. It was found that the major components of capsule and slime EPSs in terms of proteins, polysaccharides, as well as uronic acids were quite different in contents for the Fe2+-acclimated strains, and they even changed more when the Fe2+-acclimated strains were further acclimated to the other substrates. When exposed to heavy metals, all strains demonstrated much decrease in contents of capsule EPSs, and much increase in slime EPSs contents and the heavy metals were found to bound to the slime parts. It was for the first time the EPSs of the bioleaching strains were fractionated into capsule part and slime part, and it was also for the first time we found the differences in evolution of compositions and contents of the major organic components as well as the inorganic matter of capsule EPSs and slime EPSs when the bioleaching strains were exposed to different energy substrates and heavy metals.

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In Situ Characterization of Relevance of Surface Microstructure and Electrochemical Properties of Chalcopyrite to Adsorption of Acidianus manzaensis

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1130.183 ◽

2015 ◽

Vol 1130 ◽

pp. 183-187 ◽

Cited By ~ 3

Author(s):

Jin Lan Xia ◽

Hong Rui Zhu ◽

Lei Wang ◽

Hong Chang Liu ◽

Zhen Yuan Nie ◽

...

Keyword(s):

Electrochemical Properties ◽

Electrochemical Treatment ◽

Specific Adsorption ◽

Initial Oxidation ◽

Edge Structure ◽

X Ray ◽

Oxidation Potentials ◽

Before And After ◽

Acidianus Manzaensis

The in situ relevance of micro- structure and electrochemical properties of chalcopyrite to adsorption of thermoacidophilic bioleaching Archaea Acidianus manzaensis was studied. In this study, the electrochemical behavior of chalcopyrite was first investigated by cyclic voltammetry (CV) to get suitable initial reduction and oxidation potentials, at which electrochemical corrosions of chalcopyrite for several time were performed, respectively, to get specific surface micro-structures. The specific adsorption of A. manzaensis on the electrochemically corroded chalcopyrite surface was then comparatively studied. The changes of microstructure and chemical composition/speciation on the surface of chalcopyrite before and after electrochemical treatment and bio-adsorption was characterized by scanning electron microscopy/electron dispersive spectroscopy (SEM/EDS), and synchrotron radiation-based X-ray diffraction (SR-XRD) and Fe, Cu K-edge X-ray absorption near edge structure (XANES) spectroscopy. The results showed that the suitable initial oxidation and reduction of chalcopyrite electrode were at 0.67 V for 1h and -0.54 V for 10 min, respectively. After treated at 0.67V the surface of chalcopyrite became Cu-deficient with a composition of CuFe1.02S2.15, and bornite (Cu5FeS4) was detected. While after treated at -0.54V, the surface became Fe/S-deficient, with a composition of CuFe0.33S0.81, and a mass of chalcocite and some covellite were detected. Comparing to the original chalcopyrite, the adsorption capacity of A. manzaensis was increased on the surface of oxidation-treatment at 0.67 V, and decreased on the surface of reduction-treatment at -0.54 V. It clearly demonstrates the bornite-containing copper deficient chalcopyrite surface was more preferably adsorbed, whereas the chalcocite-containing Fe/S deficient chalcopyrite surface was less adsorbed by A. manzaensis, indicating the dependence of the specific adsorption of A. manzaensis upon the secondary minerals as well as Fe/S availability in the microstructure of chalcopyrite.

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Investigations of Attached and Unattached Cells during Bioleaching of Chalcopyrite with Acidianus Manzaensis at 65°C

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.71-73.377 ◽

2009 ◽

Vol 71-73 ◽

pp. 377-380

Author(s):

H. Zhou ◽

L. Zhang ◽

Y. Guo ◽

J. Peng ◽

M. Wei ◽

...

Keyword(s):

Chemical Composition ◽

Redox Potential ◽

Cell Density ◽

Morphological Feature ◽

Copper Ion ◽

Solution Chemistry ◽

Cooperative Action ◽

Shake Flasks ◽

Acidianus Manzaensis ◽

Thiosulfate Concentration

For the purpose of investigations of the different situations when the cells of Acidianus manzaensis can or can not attach the surface of the chalcopyrite. Three experiments were carried out in the modified shake flasks, and in one of the experiments the cells could not attach the surface of the chalcopyrite, but could participate in the solution chemistry of the process. The redox potential, pH, cell density, copper, ion and thiosulfate concentration in the solution were monitored in the experiments, and the morphological feature and chemical composition of the leached residues were analyzed by SEM and XRD. The most leach effiency of Cu and Fe was reached in the experiment that the Acidianus manzaensis could attach the surface of the chalcopyrite. However, the unattached Acidianus manzaensis could also leach the chalcopyrite, but it was less actively than attached cells. There was no precipitation of jarosite in the leached residues of the three experiments, but there was sulfur in the leached residues when the cells could not attach the chalcopyrite. So the surface passivating layer of sulfur can be removed only when the Acidianus manzaensis can attach the surface of the chalcopyrite. From these results it become apparent that the leach of the chalcopyrite is the cooperative action of the attached and unattached Acidianus manzaensis.

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