Persistence of Radiation-Induced Chromosome Aberrations in a Long-Term Cell Culture

✓ Long-term survivors of Hodgkin disease may develop second primary tumors caused by the mutagenic effects of radio- and chemotherapy. The authors describe the case of a 35-year-old woman who presented with an unusual meningioma of the cervical spine 9 years after undergoing combined-modality treatment for Hodgkin disease. To the authors' knowledge, this is the first report of spinal meningioma as a complication of such therapy. Whereas radiation-induced intracranial meningiomas are well described in the literature, treatment-induced meningiomas of the spine have not been widely recognized.

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Amifostine-Loaded Dissolving Armored Microneedles for Long-Term Prevention of Ionizing Radiation-Induced Injury

SSRN Electronic Journal ◽

10.2139/ssrn.3539215 ◽

2020 ◽

Author(s):

Xiang Yu ◽

Minshu Li ◽

Lin Zhu ◽

Jingfei li ◽

Guoli Zhang ◽

...

Keyword(s):

Ionizing Radiation ◽

Radiation Induced

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Cumulative incidence and risk factors for radiation induced leukoencephalopathy in high grade glioma long term survivors

Scientific Reports ◽

10.1038/s41598-021-89216-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Robert Terziev ◽

Dimitri Psimaras ◽

Yannick Marie ◽

Loic Feuvret ◽

Giulia Berzero ◽

...

Keyword(s):

Risk Factors ◽

Cumulative Incidence ◽

High Grade Glioma ◽

High Grade ◽

Nucleotide Polymorphisms ◽

Increased Risk ◽

Radiation Induced ◽

Long Term Survivors

AbstractThe incidence and risk factors associated with radiation-induced leukoencephalopathy (RIL) in long-term survivors of high-grade glioma (HGG) are still poorly investigated. We performed a retrospective research in our institutional database for patients with supratentorial HGG treated with focal radiotherapy, having a progression-free overall survival > 30 months and available germline DNA. We reviewed MRI scans for signs of leukoencephalopathy on T2/FLAIR sequences, and medical records for information on cerebrovascular risk factors and neurological symptoms. We investigated a panel of candidate single nucleotide polymorphisms (SNPs) to assess genetic risk. Eighty-one HGG patients (18 grade IV and 63 grade III, 50M/31F) were included in the study. The median age at the time of radiotherapy was 48 years old (range 18–69). The median follow-up after the completion of radiotherapy was 79 months. A total of 44 patients (44/81, 54.3%) developed RIL during follow-up. Twenty-nine of the 44 patients developed consistent symptoms such as subcortical dementia (n = 28), gait disturbances (n = 12), and urinary incontinence (n = 9). The cumulative incidence of RIL was 21% at 12 months, 42% at 36 months, and 48% at 60 months. Age > 60 years, smoking, and the germline SNP rs2120825 (PPARg locus) were associated with an increased risk of RIL. Our study identified potential risk factors for the development of RIL (age, smoking, and the germline SNP rs2120825) and established the rationale for testing PPARg agonists in the prevention and management of late-delayed radiation-induced neurotoxicity.

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Design and development of microbioreactors for long-term cell culture in controlled oxygen microenvironments

Biomedical Microdevices ◽

10.1007/s10544-011-9592-9 ◽

2011 ◽

Vol 14 (1) ◽

pp. 145-152 ◽

Cited By ~ 47

Author(s):

Hasan E. Abaci ◽

Raghavendra Devendra ◽

Quinton Smith ◽

Sharon Gerecht ◽

German Drazer

Keyword(s):

Cell Culture ◽

Design And Development

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Temperature and the Formation of Radiation-induced Chromosome Aberrations. I. The Effect of Irradiation Temperature

International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine ◽

10.1080/09553008514552911 ◽

1985 ◽

Vol 49 (4) ◽

pp. 665-672 ◽

Cited By ~ 10

Author(s):

K. Gumrich ◽

R.P. Virsik-Peuckert ◽

D. Harder

Keyword(s):

Chromosome Aberrations ◽

Irradiation Temperature ◽

Radiation Induced

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The evolving definition of salivary gland stem cells

npj Regenerative Medicine ◽

10.1038/s41536-020-00115-x ◽

2021 ◽

Vol 6 (1) ◽

Author(s):

Cecilia Rocchi ◽

Lara Barazzuol ◽

Rob P. Coppes

Keyword(s):

Stem Cells ◽

Salivary Gland ◽

Adult Stem Cell ◽

Cell Types ◽

Research Field ◽

New Therapeutic Approaches ◽

Recent Developments ◽

Radiation Induced ◽

Definition Of

AbstractDysfunction of the salivary gland and irreversible hyposalivation are the main side effects of radiotherapy treatment for head and neck cancer leading to a drastic decrease of the quality of life of the patients. Approaches aimed at regenerating damaged salivary glands have been proposed as means to provide long-term restoration of tissue function in the affected patients. In studies to elucidate salivary gland regenerative mechanisms, more and more evidence suggests that salivary gland stem/progenitor cell behavior, like many other adult tissues, does not follow that of the hard-wired professional stem cells of the hematopoietic system. In this review, we provide evidence showing that several cell types within the salivary gland epithelium can serve as stem/progenitor-like cells. While these cell populations seem to function mostly as lineage-restricted progenitors during homeostasis, we indicate that upon damage specific plasticity mechanisms might be activated to take part in regeneration of the tissue. In light of these insights, we provide an overview of how recent developments in the adult stem cell research field are changing our thinking of the definition of salivary gland stem cells and their potential plasticity upon damage. These new perspectives may have important implications on the development of new therapeutic approaches to rescue radiation-induced hyposalivation.

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Metaphase-Based Cytogenetic Approach Identifies Radiation-Induced Chromosome and Chromatid Aberrations in Zebrafish Embryos

Radiation Research ◽

10.1667/rade-21-00145.1 ◽

2021 ◽

Author(s):

Halida Thanveer Asana Marican ◽

Hongyuan Shen

Keyword(s):

Animal Model ◽

Radiation Exposure ◽

Progenitor Cells ◽

Chromosome Aberrations ◽

Structural Changes ◽

Current Knowledge ◽

Living Organism ◽

Zebrafish Embryos ◽

Radiation Induced

Metaphase-based cytogenetic methods based on scoring of chromosome aberrations for the estimation of the radiation dose received provide a powerful approach for evaluating the associated risk upon radiation exposure and form the bulk of our current knowledge of radiation-induced chromosome damages. They mainly rely on inducing quiescent peripheral lymphocytes into proliferation and blocking them at metaphases to quantify the damages at the chromosome level. However, human organs and tissues demonstrate various sensitivity towards radiation and within them, self-proliferating progenitor/stem cells are believed to be the most sensitive populations. The radiation-induced chromosome aberrations in these cells remain largely unknown, especially in the context of an intact living organism. Zebrafish is an ideal animal model for research into this aspect due to their small size and the large quantities of progenitor cells present during the embryonic stages. In this study, we employ a novel metaphase-based cytogenetic approach on zebrafish embryos and demonstrate that chromosome-type and chromatid-type aberrations could be identified in progenitor cells at different cell-cycle stages at the point of radiation exposure. Our work positions zebrafish at the forefront as a useful animal model for studying radiation-induced chromosome structural changes in vivo.

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