scholarly journals Corticosteroid receptors involved in stress regulation in common carp, Cyprinus carpio

2008 ◽  
Vol 198 (2) ◽  
pp. 403-417 ◽  
Author(s):  
Ellen H Stolte ◽  
Aurélia F de Mazon ◽  
Karen M Leon-Koosterziel ◽  
Maria Jesiak ◽  
Nic R Bury ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Bioinformatic Analysis ◽  
Duplication Event ◽  
Stress Axis ◽  
Mrna Levels ◽  
Stress Regulation ◽  
Regulatory Processes ◽  
The Brain ◽  
Higher Sensitivity

In higher vertebrates, mineralo- (aldosterone) and glucocorticoids (cortisol/corticosterone) exert their multiple actions via specific transcription factors, glucocorticoid (GR) and mineralocorticoid (MR) receptors. Teleostean fishes lack aldosterone and mineral regulatory processes seem under dominant control by cortisol. Despite the absence of the classical mineralocorticoid aldosterone, teleostean fishes do have an MR with cortisol and possibly 11-deoxycorticosterone (DOC) (as alternative for aldosterone) as predominant ligands. We studied corticoid receptors in common carp (Cyprinus carpio L). Through homology cloning and bioinformatic analysis, we found duplicated GR genes and a single MR gene. The GR genes likely result from a major genomic duplication event in the teleostean lineage; we propose that the gene for a second MR was lost. Transactivation studies show that the carp GRs and MR have comparable affinity for cortisol; the MR has significantly higher sensitivity to DOC, and this favours a role for DOC as MR ligand in fish physiology. mRNA of the GRs and the MR is expressed in forebrain (in pallial areas homologous to mammalian hippocampus), corticotrophin-releasing hormone (CRH) cells in the pre-optic nucleus (NPO) and pituitary pars distalis ACTH cells, three key neural/endocrine components of the stress axis. After exposure to prolonged and strong (not to mild acute) stressors, mRNA levels of both GRs and MR become down-regulated in the brain, but not in the NPO CRH cells or pituitary ACTH cells. Our data predicts a function in stress physiology for all CRs and suggest telencephalon as a first line cortisol target in stress.

scholarly journals Experimental hyperthyroidism and central mediators of stress axis and thyroid axis activity in common carp (Cyprinus carpio L.)

2006 ◽  
Vol 37 (3) ◽  
pp. 443-452 ◽  
Author(s):  
Edwin J W Geven ◽  
Folkert Verkaar ◽  
Gert Flik ◽  
Peter H M Klaren
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Plasma Cortisol ◽  
Stress Axis ◽  
Pars Intermedia ◽  
Mrna Levels ◽  
Experimental Hyperthyroidism ◽  
Pomc Mrna ◽  
Hpi Axis ◽  
Thyroid Axis

The effect of experimental hyperthyroidism, realized by T4 injection, on central mediators of the hypothalamo–pituitary–interrenal axis (HPI-axis) in common carp (Cyprinus carpio L.) was studied. Our results show that hyperthyroidism evokes a marked 3.2-fold reduction in basal plasma cortisol levels. Corticotropin-releasing hormone-binding protein (CRH-BP) mRNA levels in the hypothalamus, measured by real-time quantitative PCR, were significantly elevated by 40%, but CRH, urotensin-I, prepro-TRH, prohormone convertase-1 (PC1), and POMC mRNA levels were unchanged. In the pituitary pars distalis, PC1, CRH receptor-1, and POMC mRNA levels were unaffected, as was ACTH content. Plasma α-MSH concentrations were significantly elevated by 30% in hyperthyroid fish, and this was reflected in PC1 and POMC mRNA levels in pituitary pars intermedia that were increased 1.5- and 2.4-fold respectively. The α-MSH content of the pars intermedia was unchanged. Hyperthyroidism has profound effects on the basal levels of a central mediator, i.e., CRH-BP, of HPI-axis function in unstressed carp in vivo, and we conclude that HPI- and hypothalamo–pituitary–thyroid-axis functions are strongly interrelated. We suggest that the changes in plasma cortisol, thyroid hormone, and α-MSH levels reflect their concerted actions on energy metabolism.

The blood and mRNA levels of antioxidant-related factors in common carp (Cyprinus carpio) fed p-Coumaric acid

2020 ◽  
Author(s):  
Ehsan Ahmadifar ◽  
Naser Kalhor ◽  
Mahmoud A. O. Dawood ◽  
Mehdi Ahmadifar ◽  
Mohsen Shahriari Moghadam ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Mrna Levels ◽  
Coumaric Acid ◽  
Related Factors ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

Recombinant human leptin attenuates stress axis activity in common carp (Cyprinus carpio L.)

2012 ◽  
Vol 178 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Marnix Gorissen ◽  
Nicholas J. Bernier ◽  
Remy Manuel ◽  
Stefan de Gelder ◽  
Juriaan R. Metz ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Stress Axis ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

scholarly journals Central and peripheral interleukin-1β and interleukin-1 receptor I expression and their role in the acute stress response of common carp, Cyprinus carpio L.

2006 ◽  
Vol 191 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Juriaan R Metz ◽  
Mark O Huising ◽  
Karin Leon ◽  
B M Lidy Verburg-van Kemenade ◽  
Gert Flik
Keyword(s):  
Common Carp ◽  
Hpa Axis ◽  
Cyprinus Carpio ◽  
Acute Stress ◽  
Expression Profiles ◽  
Head Kidney ◽  
Interleukin 1Β ◽  
Pars Intermedia ◽  
Mrna Levels ◽  
Hpi Axis

In fish, the hypothalamus–pituitary–interrenal axis (HPI-axis), the equivalent of the hypothalamus–pituitary–adrenal axis (HPA-axis) in mammals, is activated during stress and leads to production and release of cortisol by the interregnal cells in the head kidney. In mammals, the cytokine interleukin-1β (IL-1β) takes a key position in the innate immune and inflammatory responses and influences the HPA-axis. In fish, studies that address the effects of cytokines on HPI-axis activation are limited. We quantitatively assessed expression of IL-1β and its receptor, IL-1RI (the latter was cloned and sequenced), in an acute restraint stress paradigm in common carp, Cyprinus carpio. We also considered expression of the pituitary hormones prolactin (PRL) and GH that have been shown to be structurally related to cytokines and have immunomodulatory actions. Pituitary PRL expression increased fourfold during stress; GH mRNA levels were unaffected. Following restraint, hypothalamic IL-1β expression was upregulated; in head kidney and pituitary pars intermedia, IL-1RI expression significantly increased. We suggest that during acute stress IL-1β signalling in the HPI-axis becomes more sensitive, since both ligand and receptor expressions are enhanced. In vitro, recombinant carp IL-1β stimulates release of α-MSH and N-Ac β-endorphin from the pituitary gland. This observation concurs with increased in vivo plasma levels of α-MSH and N-Ac β-endorphin following restraint. Our findings combined lead us to conclude that IL-1β affects the activity of the HPI-axis and, in turn, expression profiles of genes encoding IL-1β and its receptor are modified during acute stress. Our study provides convincing evidence for bi-directional communication of the HPI-axis and the immune system in fish.

Mancozeb exposure results in manganese accumulation and Nrf2-related antioxidant responses in the brain of common carp Cyprinus carpio

2018 ◽  
Vol 25 (16) ◽  
pp. 15529-15540 ◽  
Author(s):  
Dennis Guilherme Costa-Silva ◽  
Andressa Rubim Lopes ◽  
Illana Kemmerich Martins ◽  
Luana Paganotto Leandro ◽  
Mauro Eugênio Medina Nunes ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Antioxidant Responses ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
The Brain

scholarly journals Quantification of common carp (Cyprinus carpio) IGF-I and IGF-II mRNA by real-time PCR: differential regulation of expression by GH

2003 ◽  
Vol 178 (3) ◽  
pp. 513-521 ◽  
Author(s):  
QP Vong ◽  
KM Chan ◽  
CH Cheng
Keyword(s):  
Real Time ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Mrna Levels ◽  
Regulation Of Expression ◽  
Taq Polymerase ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Igf I ◽  
Extrahepatic Tissues

IGFs are potent mitogens for many different cell types and play important roles in growth and development. A multitude of regulatory factors modulate the expression of IGFs. In some teleosts, liver IGF-I expression has been demonstrated to depend on the presence of GH. However, the GH dependence of IGF-II expression in teleosts is controversial. Moreover, most IGF expression studies in bony fish have been focused on the liver, and information on extrahepatic tIssues are conflicting and inconsistent. This is partly due to the fact that the traditional methods of mRNA measurement such as Northern blot and RT-PCR are not sensitive enough to detect changes in IGF levels in extrahepatic tIssues because of the low levels of IGFs in these tIssues. In addition, there have been few studies on the IGF system of non-salmonid teleosts. Our laboratory has thus begun such studies on a local tropical fast-growing fish, the common carp (Cyprinus carpio). In this study, real-time quantitative PCR assays were developed for the accurate measurement of IGF-I and IGF-II mRNA levels in common carp tIssues. This quantitative method was based on the measurement of a fluorescent labeled probe, which was cleaved by Taq polymerase during PCR by the 5'-->3' nuclease activity. The signal generated was directly proportional to the starting copy number of the target molecules in the sample. Hence, it was possible to detect and quantify the mRNA levels of both IGF-I and IGF-II reliably in very small amounts of tIssues obtained from juvenile common carp. Using these assays, the expression pattern of IGF-I and IGF-II in various common carp tIssues was studied, and their differential response to GH stimulation was also investigated.

Screening cold-acclimation differential expression candidate genes in the brain of common carp (Cyprinus carpio)

2011 ◽  
Vol 33 (3) ◽  
pp. 262-269 ◽  
Author(s):  
Li-Hua XU ◽  
Yu-Mei CHANG ◽  
Chun-Lei LIU ◽  
Li-Qun LIANG ◽  
Jin-Liang LIU ◽  
...  
Keyword(s):  
Cold Acclimation ◽  
Differential Expression ◽  
Candidate Genes ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
The Brain
Sign in / Sign up

Export Citation Format

Share Document