THE LEVELS OF FSH AND LH IN THE PITUITARY OF THE EWE IN RELATION TO FOLLICULAR GROWTH AND OVULATION

1962 ◽  
Vol 24 (2) ◽  
pp. 143-151 ◽  
Author(s):  
H. A. ROBERTSON ◽  
J. S. M. HUTCHINSON
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Ovarian Follicles ◽  
Anterior Lobe ◽  
The State ◽  
Oestrous Cycle ◽  
Follicular Growth ◽  
Ovulatory Period ◽  
Marked Drop ◽  
Stimulating Hormone

SUMMARY The levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) have been determined in the anterior lobe of the pituitary of ewes killed at precise stages of the oestrous cycle, during anoestrus, pregnancy, lactation, and in virgin ewes. A marked drop in the content of both FSH and LH has been demonstrated during the ovulatory period of the cycle. The values obtained are discussed in relation to the state of the ovarian follicles.

OESTROGEN SYNTHESIS IN RAT OVARIES IN VITRO: EFFECTS OF ENDOGENOUS AND EXOGENOUS GONADOTROPHINS

1972 ◽  
Vol 53 (3) ◽  
pp. 397-406 ◽  
Author(s):  
BRENDA ROBINSON ◽  
R. E. OAKEY
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Oestrous Cycle ◽  
The Other ◽  
Ovarian Vein ◽  
Hormone Activity ◽  
In Vitro Effects ◽  
Stimulating Hormone

SUMMARY The rate of synthesis of [14C]oestrone and [14C]oestradiol-17β from [14C]testosterone in vitro by ovaries from rats at different stages of the oestrous cycle was measured. The rate of [14C]oestrogen synthesis was highest in ovaries taken from rats in pro-oestrus and lowest in ovaries taken from rats early in the dioestrous phase of the cycle. Rates of synthesis in ovaries obtained from rats in the late dioestrous stage were intermediate between the rates of the other groups. The rates of [14C]oestrogen synthesis at these periods of the cycle paralleled the concentrations of oestrogens in ovarian vein plasma reported by other authors. Gonadotrophin preparations with either luteinizing hormone activity or both follicle-stimulating hormone and luteinizing hormone activities had no effect on [14C]oestrogen synthesis by rat ovaries in vitro at any of these stages of the oestrous cycle.

scholarly journals Changes in the Concentration of Gonadotrophic and Steroidal Hormones in the Antral Fluid of Ovarian Follicles Throughout the Oestrous Cycle of the Sheep

1981 ◽  
Vol 34 (1) ◽  
pp. 67 ◽  
Author(s):  
KP McNatty ◽  
M Gibb ◽  
C Dobson ◽  
DC Thurley ◽  
JK Findlay
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Ovarian Follicles ◽  
Oestrous Cycle ◽  
Steroid Secretion ◽  
Steroidal Hormones ◽  
Follicle Size ◽  
The Individual ◽  
Secretion Rates ◽  
Venous Plasma

The concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, progesterone, androstenedione and oestradiol were determined in the antral fluid of ovarian follicles > 1 mm in diameter as well as in ovarian venous or peripheral venous plasma, or both, from at least four different animals on each day throughout the oestrous cycle of the sheep. The individual steroid hormones in antral fluid were examined in relation to the steroid-secretion rates in ovarian venous plasma, follicle size and the hormone levels in jugular venous plasma.

scholarly journals The Roles of Luteinizing Hormone, Follicle-Stimulating Hormone and Testosterone in Spermatogenesis and Folliculogenesis Revisited

2021 ◽  
Vol 22 (23) ◽  
pp. 12735
Author(s):  
Olayiwola O. Oduwole ◽  
Ilpo T. Huhtaniemi ◽  
Micheline Misrahi
Keyword(s):  
Luteinizing Hormone ◽  
Leydig Cells ◽  
Low Dose ◽  
Follicle Stimulating Hormone ◽  
Rodent Models ◽  
Follicular Growth ◽  
Lh Receptor ◽  
Transgenic Expression ◽  
Stimulating Hormone

Spermatogenesis and folliculogenesis involve cell–cell interactions and gene expression orchestrated by luteinizing hormone (LH) and follicle-stimulating hormone (FSH). FSH regulates the proliferation and maturation of germ cells independently and in combination with LH. In humans, the requirement for high intratesticular testosterone (T) concentration in spermatogenesis remains both a dogma and an enigma, as it greatly exceeds the requirement for androgen receptor (AR) activation. Several data have challenged this dogma. Here we report our findings on a man with mutant LH beta subunit (LHβ) that markedly reduced T production to 1–2% of normal., but despite this minimal LH stimulation, T production by scarce mature Leydig cells was sufficient to initiate and maintain complete spermatogenesis. Also, in the LH receptor (LHR) knockout (LuRKO) mice, low-dose T supplementation was able to maintain spermatogenesis. In addition, in antiandrogen-treated LuRKO mice, devoid of T action, the transgenic expression of a constitutively activating follicle stimulating hormone receptor (FSHR) mutant was able to rescue spermatogenesis and fertility. Based on rodent models, it is believed that gonadotropin-dependent follicular growth begins at the antral stage, but models of FSHR inactivation in women contradict this claim. The complete loss of FSHR function results in the complete early blockage of folliculogenesis at the primary stage, with a high density of follicles of the prepubertal type. These results should prompt the reassessment of the role of gonadotropins in spermatogenesis, folliculogenesis and therapeutic applications in human hypogonadism and infertility.

DEVELOPMENT AND APPLICATION OF HOMOLOGOUS RADIOIMMUNOASSAYS FOR PORCINE GONADOTROPHINS

1979 ◽  
Vol 81 (1) ◽  
pp. 1-10 ◽  
Author(s):  
J. L. VANDALEM ◽  
CH. BODART ◽  
G. PIRENS ◽  
J. CLOSSET ◽  
G. HENNEN
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Oestrous Cycle ◽  
Releasing Hormone ◽  
Porcine Serum ◽  
Lh Releasing Hormone ◽  
Stimulating Hormone

Antisera were raised against highly purified preparations of porcine luteinizing hormone (pLH) and follicle-stimulating hormone (pFSH). Highly specific and sensitive radioimmunoassay systems were developed. The antisera to LH and FSH were used at working dilutions of 1: 500 000 and 1: 200 000 respectively and the sensitivities of the assays were 0·1 ng LH/ml serum (3 × 10−12 mol/l) and 0·5 ng FSH/ml serum (1·5 × 10−11 mol/l). The LH and FSH preparations used as standards were 1·2 and 81 times as potent as NIH-LH-S15 and NIH-FSH-P1 respectively. Both assays were validated and adapted for the measurement of the gonadotrophin content of porcine serum. The concentrations of LH and FSH in blood were measured simultaneously in prepubertal sows throughout a 24 h period, in adult sows during the oestrous cycle and in both prepubertal and adult animals after treatment with LH releasing hormone.

EFFECT OF OVINE LUTEINIZING HORMONE ON THE SECRETION OF PROGESTERONE IN VITRO BY OVARIAN FOLLICLES FROM HYPOPHYSECTOMIZED RATS TREATED WITH FOLLICLE-STIMULATING HORMONE

1978 ◽  
Vol 77 (3) ◽  
pp. 419-420
Author(s):  
A. R. LABARBERA ◽  
MERO R. NOCENTI
Keyword(s):  
New York ◽  
Luteinizing Hormone ◽  
Corn Oil ◽  
Follicle Stimulating Hormone ◽  
Virgin Female ◽  
Ovarian Follicles ◽  
Female Rats ◽  
Hypophysectomized Rats ◽  
Stimulating Hormone

Department of Physiology, College of Physicians and Surgeons, Columbia University, New York 10032, U.S.A. (Received 14 November 1977) Ovarian follicles from oestrous, pro-oestrous and hypophysectomized rats have the capacity to secrete progestins in vitro and to respond to luteinizing hormone (LH) by increasing this secretion (Stoklosowa & Nalbandov, 1972; LaBarbera, Nocenti & Castellano, 1974; Lindner, Tsafriri, Lieberman, Zor, Koch, Bauminger & Barnea, 1974). Follicles from hypophysectomized rats, untreated or treated with follicle-stimulating hormone (FSH), were therefore studied in experiments in vitro to investigate further the gonadotrophic control of progesterone secretion. Mature virgin female rats (200–250 g) were hypophysectomized and, beginning on day 4 after the operation, received a total of 360 μg ovine FSH (NIH-FSH-S9)/100 g body weight, injected s.c. in corn oil as six divided doses, one every 12 h for 3 days. Untreated controls received corn oil only. On day 7 after hypophysectomy, the ovaries were excised and

Release of 3H2O from 1β,2β[3H]androstenedione by equine granulosa cells

1983 ◽  
Vol 104 (2) ◽  
pp. 227-232 ◽  
Author(s):  
E. V. YoungLai ◽  
J. F. Jarrell
Keyword(s):  
Luteinizing Hormone ◽  
Granulosa Cells ◽  
Negative Correlation ◽  
Follicular Fluid ◽  
Enzyme System ◽  
Follicle Stimulating Hormone ◽  
Significant Negative Correlation ◽  
Oestrous Cycle ◽  
Bicarbonate Buffer ◽  
Stimulating Hormone

Abstract. Granulosa cells were harvested from mares at various stages of the oestrous cycle and incubated in Krebs-Ringer bicarbonate buffer with 1β,2β[3H]androstenedione as substrate. The release of 3H2O expressed as cpm/h/mg protein varied from 44 000 to 768 000 in follicles from 7 mares. The release of 3H2O was not significantly altered by luteinizing hormone, follicle stimulating hormone or pregnant mare's serum gonadotrophin. There was a significant negative correlation between the release of 3H2O and the concentration of progesterone in the follicular fluid. Based on the assumption that the release of 3H2O represent total aromatization, these data suggest that the equine granulosa cells have a very active aromatizing enzyme system.

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