PROGESTERONE CONCENTRATION IN THE PERIPHERAL PLASMA OF PREGNANT GOATS

1972 ◽  
Vol 53 (3) ◽  
pp. 447-452 ◽  
Author(s):  
G. IRVING ◽  
D. E. JONES ◽  
A. KNIFTON
Keyword(s):  
Cord Blood ◽  
Venous Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Peripheral Plasma ◽  
Significant Difference ◽  
Competitive Protein Binding ◽  
The Mean ◽  
Serial Samples ◽  
Twin Pregnancies

SUMMARY Plasma progesterone concentration was measured by a competitive protein-binding method in serial samples of jugular venous blood from 21 pregnant goats, 11 with twin and 10 with single foetuses. Progesterone levels in twin pregnancies were significantly greater than in singletons. The mean progesterone concentration (ng/ml plasma) in the twin pregnancies was greatest during the 3rd month of gestation (10·7 ± 0·4 (s.e.m.)) and in the singletons during the 4th month (7·8 ± 0·2 (s.e.m.)). There was a significant decrease in mean progesterone concentration in the last month of pregnancy due to a steady decline in the last 7 days before parturition. The mean progesterone concentrations at parturition in five twin and eight single pregnancies were 2·2 ± 0·4 and 1·5 ± 0·2 (s.e.m.) ng/ml plasma respectively; there was no significant difference between these values. In cord blood from nine kids immediately after delivery the progesterone concentration was 0·9 ± 0·1 ng/ml.

THE PROGESTERONE CONCENTRATION IN THE PLASMA OF THE GOAT DURING THE OESTROUS CYCLE AND PREGNANCY

1972 ◽  
Vol 52 (1) ◽  
pp. 23-36 ◽  
Author(s):  
G. D. THORBURN ◽  
W. SCHNEIDER
Keyword(s):  
Binding Assay ◽  
Progesterone Concentration ◽  
Maternal Circulation ◽  
Plasma Progesterone ◽  
Pregnant Uterus ◽  
Main Site ◽  
Peripheral Plasma ◽  
Protein Binding Assay ◽  
The Mean ◽  
Mean Concentration

SUMMARY Progesterone concentrations in the peripheral plasma of goats were measured by a protein-binding assay. The mean concentration was extremely low on the day of oestrus (0·2 ng/ml) and was not significantly different from that found in anoestrous or ovariectomized animals. The concentration increased to a maximum of 4 ng/ml on about day 10 of the 21-day cycle, and decreased rapidly during the last 3 days of the cycle. Plasma progesterone concentration during early pregnancy (2·5–3·5 ng/ml) was similar to the luteal phase value and remained steady from day 8 to day 60. Between days 60 and 70 there was a secondary increase in progesterone concentration which was maintained at this increased level (4·5–5·5 ng/ml) until just before parturition. In twin-bearing animals, the secondary increase was greater. Progesterone concentration decreased rapidly during the 1–2 days preceding parturition, but the concentration was still quite high on the day of parturition (1·25 ng/ml). The progesterone concentration in peripheral plasma was markedly increased during anaesthesia and the operation. After bilateral ovariectomy of the pregnant goat, peripheral progesterone concentration fell rapidly from 9 to 2·5 ng/ml during the first ½ h and then more slowly during the next 5–6 h. The animals aborted 36–48 h later. A consistent positive arterio—venous difference for progesterone was observed across the pregnant uterus in two unanaesthetized goats. These results indicate that the ovary is the main site of progesterone production in the pregnant goat and that production by the placenta is small and unlikely to influence the level of this hormone in the maternal circulation.

scholarly journals Selected Factors that Affect the Measurement of Plasma Progesterone Concentrations in Pregnant Ewes

1974 ◽  
Vol 27 (6) ◽  
pp. 659 ◽  
Author(s):  
AR Gleeson ◽  
GD Thorburn
Keyword(s):  
Protein Binding ◽  
Diurnal Variation ◽  
Significant Positive Correlation ◽  
Plasma Samples ◽  
Elevated Plasma ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Peripheral Plasma ◽  
Competitive Protein Binding

A competitive protein-binding technique was used to measure progesterone concentrations in the peripheral plasma of pregnant ewes. Neither haemolysis of blood nor thawing of plasma samples affected plasma progesterone concentration. Blood samples should be chilled immediately upon collection but subsequent to centrifugation immediate chilling of the plasma samples is not critical. No consistent diurnal variation in progesterone concentrations was evident but there was large apparently random day-to-day variation in progesterone concentrations for any ewe. Although a significant positive correlation was found between endogenous progesterone and corticosteroid concentrations, the present study failed to correlate experimentally elevated plasma corticosteroid concentrations with progesterone concentrations. Progesterone concentrations varied greatly between ewes at the same stage of pregnancy.

PLASMA PROGESTERONE LEVELS IN GOATS DURING PREGNANCY MEASURED BY COMPETITIVE PROTEIN BINDING

1971 ◽  
Vol 66 (3) ◽  
pp. 471-477 ◽  
Author(s):  
Anne Kristine Blom ◽  
Olav Lyngset
Keyword(s):  
Protein Binding ◽  
Uterine Artery ◽  
Venous Blood ◽  
Ovarian Vein ◽  
Progesterone Concentration ◽  
Gradual Decline ◽  
Plasma Progesterone ◽  
Peripheral Venous Blood ◽  
Before And After ◽  
Competitive Protein Binding

ABSTRACT Plasma progesterone concentration at different stages of pregnancy was measured in the blood obtained from the uterine artery (6 goats), the uterine vein (6 goats), and the ovarian vein (11 goats). Progesterone concentration was also measured in the peripheral venous blood before and after extirpation of the ovaries and the uterus (16 goats). The concentration of progesterone was found to rise gradually in the peripheral and ovarian vein blood to reach a maximum at 90 days of pregnancy. This was followed by a gradual decline with consistent low values of about 7 ng prog./ml 3–4 days before parturition. A significant decrease in peripheral progesterone concentration was found 10 minutes after removing the ovaries and the uterus. In two goats sampled before and after parturition, the plasma progesterone concentration was found to decrease before parturition and to remain low for at least three days after parturition.

Postpartum Changes in the Minimum Alveolar Concentration of Isoflurane

1995 ◽  
Vol 82 (6) ◽  
pp. 1360-1363. ◽  
Author(s):  
Matthew T. V. Chan ◽  
Tony Gin
Keyword(s):  
Minimum Alveolar Concentration ◽  
Normal Values ◽  
Venous Blood ◽  
Rank Correlation ◽  
Tubal Ligation ◽  
Progesterone Concentration ◽  
Electric Stimulus ◽  
Plasma Progesterone ◽  
The Mean ◽  
End Tidal

Background Minimum alveolar concentration (MAC) is decreased in pregnancy, but it is not known how quickly after delivery MAC returns to normal. We measured the MAC of isoflurane in a group of women undergoing elective tubal ligation after delivery. Methods After delivery, 20 patients underwent inhalational induction of anesthesia with isoflurane and tracheal intubation. MAC was determined in each patient by observing the response to a 10-s, 50-Hz, 80-mA transcutaneous tetanic electric stimulus to the ulnar nerve at various concentrations of isoflurane. The end-tidal concentration of isoflurane was kept constant for at least 10 min before each stimulus, and the concentration of isoflurane was ultimately varied in steps of 0.05 vol% until we obtained a sequence of three alternate responses: move-not move-move or not move-move-not move. The MAC for each subject was taken as the mean of the two concentrations just permitting and just preventing movement. A venous blood sample was taken immediately before induction of anesthesia for measurement of progesterone concentration. MAC was compared with time after delivery and plasma progesterone concentrations by Kendall's rank correlation. Results There was a positive correlation between MAC and the time after delivery (P < 0.001). The median MAC of isoflurane was 0.775 vol% (range 0.675-0.775 vol%) in five women 24-36 h postpartum. MAC was more variable, 0.825 vol% (0.675-0.975 vol%) in nine women 36-72 h postpartum, whereas six patients more than 72 h postpartum had a MAC of 1.125 vol% (1.025-1.175 vol%). The correlation between MAC and plasma progesterone concentration was almost statistically significant (P = 0.060). Conclusions The MAC of isoflurane was reduced in women 24-36 h postpartum and gradually increased to normal values by 72 h postpartum.

DIFFERENCE IN CONCENTRATION OF PROGESTERONE IN PLASMA BETWEEN PREGNANT AND NON-PREGNANT QUOKKAS (SETONIX BRACHYURUS)

1980 ◽  
Vol 84 (1) ◽  
pp. 153-158 ◽  
Author(s):  
M. H. CAKE ◽  
F. J. OWEN ◽  
S. D. BRADSHAW
Keyword(s):  
Luteal Phase ◽  
Oestrous Cycle ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Peripheral Plasma ◽  
Early Stages ◽  
Significant Difference ◽  
Early Peak

The plasma progesterone concentrations during pregnancy and the oestrous cycle of the quokka were measured daily after each had been initiated by the removal of pouch young. Progesterone levels ranged from 0·6 ng/ml in the early stages of the oestrous cycle to about 2·5 ng/ml at the peak of the luteal phase. There was no significant difference between pregnant and non-pregnant states before the removal of the pouch young nor in the latter half of the cycle. However, the plasma progesterone concentration on days 3–4 after removal of the pouch young was significantly greater in pregnant animals when compared with nonpregnant animals at the same stage and also when compared with the levels before removal of young. This early peak in the concentration of progesterone in peripheral plasma is discussed in relation to the development of the previously dormant blastocyst.

THE EFFECT OF PREGNANCY, OVARIECTOMY AND PARTURITION ON PLASMA PROGESTERONE LEVEL IN SHEEP

1970 ◽  
Vol 65 (2) ◽  
pp. 273-283 ◽  
Author(s):  
Petter Fylling
Keyword(s):  
Plasma Levels ◽  
Gradual Increase ◽  
Mean Value ◽  
Progesterone Level ◽  
Maximum Plasma ◽  
Plasma Progesterone ◽  
Peripheral Plasma ◽  
Marked Decline ◽  
The Mean ◽  
Twin Pregnancies

ABSTRACT In sheep a gradual increase in the progesterone* level in peripheral plasma was observed during pregnancy. The maximum plasma levels were reached at about day 140, the mean value in two ewes, each carrying one lamb being 13.2 ng/ml, and in four twin pregnancies 20.0 ng/ml. During the last week of pregnancy a marked decline in plasma progesterone levels in all the intact animals was observed with a mean value of 2.1 ng/ml at parturition. Following ovariectomy the increase in plasma progesterone in advancing stages of pregnancy which has been observed in intact animals, was blocked. In the ovariectomized animals too a decrease in plasma progesterone levels prior to parturition was demonstrated.

PROGESTERONE IN THE HUMAN PERIPHERAL BLOOD IN THE PREOVULATORY PERIOD OF THE MENSTRUAL CYCLE

1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Graafian Follicle ◽  
The Mean ◽  
Mean Concentration

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.

Investigation of the Association Between Carotid Artery Intima-Media Thickness (IMT) and Cardiac Risk Factors in Patients with Systemic Lupus Erythematosus

2020 ◽  
Vol 16 (2) ◽  
pp. 125-133
Author(s):  
Zahra Rezaieyazdi ◽  
Sima Sedighi ◽  
Masoumeh Salari ◽  
Mohammadreza H. Fard ◽  
Mahmoud R. Azarpazhooh ◽  
...  
Keyword(s):  
Risk Factors ◽  
Insulin Resistance ◽  
Lupus Erythematosus ◽  
Serum Insulin ◽  
Venous Blood ◽  
Test Group ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Background: The relationship between SLE and traditional risk factors for cardiovascular events was evaluated. Methods: The data regarding sixty patients with SLE and 30 healthy controls (age and sex matched) were gathered using SLEDAI forms. Venous blood (10mL) from all the participants was examined for hs-CRP, homocysteine, VCAM1, CBC, anti-DNA antibody, C3, C4, low-density lipoprotein (LDL), cholesterol, FBS and triglyceride. : The IMT of carotid arteries was determined bilaterally by ultrasound. Other measurements included insulin levels via Elisa (Linco/Millipore Corp) and the HOMA-IR index for insulin resistance. Results: The mean age (in years) in the test and control groups was 28.8±10.3 (18-52) and 33.8±9.13 (18-48), respectively. Results: The mean age (in years) in the test and control groups was 28.8±10.3 (18-52) and 33.8±9.13 (18-48), respectively. : The average IMT in the test group was directly related to serum levels of VCAM1 (p<0.001), homocysteine (p<0.001), cholesterol (p<0.009), LDL (p<0.001), TG (p<0.001), and FPG (p=0.004). The association between other risk factors, insulin resistance, carotid IMT and SLEDAI, was nonexistent. Mean insulin and insulin resistance levels in all the participants were 0.43±2.06 µU/mL and 0.09±0.44, respectively. There was no significant difference between the test and control groups regarding serum insulin and insulin resistance levels (p=0.42 and p=0.9, respectively). None of the risk factors, such as hsCRP, VCAM1, or homocysteine, were shown to be related to insulin resistance (p=0.6, p=0.6, p=0.09, respectively). Conclusion:: Our findings did not show an increase in the prevalence of atherosclerosis in patients with SLE. There was no association between IMT and insulin resistance. However, the former was associated with FPG, total cholesterol, LDL, TG, homocystein and VCAM1.

SECRETION OF DIHYDROTESTOSTERONE BY HUMAN PROSTATE IN BENIGN PROSTATIC HYPERTROPHY

1974 ◽  
Vol 77 (2) ◽  
pp. 401-407 ◽  
Author(s):  
J. A. Mahoudeau ◽  
A. Delassalle ◽  
H. Bricaire
Keyword(s):  
Plasma Levels ◽  
Benign Prostatic Hypertrophy ◽  
Prostatic Hypertrophy ◽  
Human Prostate ◽  
Control Subjects ◽  
Peripheral Plasma ◽  
Significant Difference ◽  
Before And After ◽  
The Mean ◽  
And Control

ABSTRACT Plasma levels of testosterone (T) and 5α-dihydrotestosterone (DHT) were determined by radioimmunoassay in 29 patients with benign prostatic hypertrophy (BPH) and in 56 control men of various ages. No significant difference was found in T, DHT nor DHT/T ratio between BPH and control subjects of similar age. Plasma DHT was higher in the prostatic than in the peripheral veins in 8/9 patients with BPH during laparotomy, indicating a prostatic secretion of DHT. No difference in the mean T nor the mean DHT was found in peripheral plasma before and after adenomectomy.

PROGESTERONE IN THE UTERINE VENOUS BLOOD OF WOMEN DURING THE FIRST HALF OF GESTATION

1963 ◽  
Vol 27 (3) ◽  
pp. 333-336 ◽  
Author(s):  
F. FUCHS ◽  
ANNA-RIITTA FUCHS ◽  
R. V. SHORT
Keyword(s):  
Peripheral Blood ◽  
Venous Blood ◽  
Progesterone Concentration ◽  
The Mean

SUMMARY Progesterone determinations were carried out in twenty-eight samples of uterine venous blood collected from women between the 9th and 22nd weeks of pregnancy. The mean progesterone concentration was 35·4 μg./100 ml. plasma (range 8–97), which is ten times as high as the concentration in the peripheral blood.

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