A RE-EXAMINATION OF THE TADPOLE IN METAMORPHIC STASIS AS A RECIPIENT IN A BIOASSAY FOR THYROID-STIMULATING HORMONE

1974 ◽  
Vol 61 (1) ◽  
pp. 15-19 ◽  
Author(s):  
MELVIN CHING
Keyword(s):  
Growth Hormone ◽  
Luteinizing Hormone ◽  
Thyroid Function ◽  
Anterior Pituitary ◽  
Rana Pipiens ◽  
Thyroid Stimulating Hormone ◽  
Pituitary Extract ◽  
Metamorphic Stage ◽  
Stimulating Hormone

SUMMARY The tadpole, in metamorphic stasis, has been used as a bioassay recipient for thyroid-stimulating hormone (TSH). The validity of its use for this purpose, however, has not been tested critically, particularly with respect to the effect of other hormones, most notably, growth hormone (GH) and luteinizing hormone (LH). Growth hormone has been shown to influence thyroid function and LH is a common contaminant of pituitary TSH preparations. Tadpoles of Rana pipiens, arrested at a particular metamorphic stage, received various concentrations of LH alone, a combination of doses of GH and TSH, or GH and rat anterior pituitary extract. Growth hormone was ineffective in re-inducing metamorphosis in 78% of cases, whereas LH stimulated metamorphosis in the tadpoles.

Monolayer Cultures of Dispersed Cells from Bovine Anterior Pituitary: Responses to Synthetic Hypophysiotropic Peptides

1975 ◽  
Vol 53 (6) ◽  
pp. 1094-1098 ◽  
Author(s):  
G. Queen ◽  
S. Vivian ◽  
F. LaBella
Keyword(s):  
Growth Hormone ◽  
Luteinizing Hormone ◽  
Anterior Pituitary ◽  
Control Level ◽  
Thyroid Stimulating Hormone ◽  
Releasing Hormone ◽  
Cell Monolayers ◽  
Pituitary Glands ◽  
Lh Rh ◽  
Stimulating Hormone

Cells were dispersed from bovine anterior pituitary glands, by digestion with collagenase, and cultured. After 4 days the cell monolayers were incubated with fresh medium containing synthetic hypophysiotropic peptides for 2, 6, or 20 h, and hormone released into the medium was estimated by radioimmunoassay. After 2 h, thyroid releasing hormone (TRH) stimulated the release of thyroid-stimulating hormone (TSH) up to eightfold, and of prolactin (PRL) and follicle-stimulating hormone (FSH) about twofold at a minimal effective concentration of 1 ng/ml; enhanced growth hormone (GH) release was not apparent until 20 h, and release of luteinizing hormone (LH) and adrenocorticotrophic hormone (ACTH) was unaffected. Luteinizing hormone releasing hormone (LH-RH) enhanced release of LH maximally (three- to fourfold) during a 2 h incubation and was effective at 0.1 ng/ml; FSH release was significantly enhanced by about 50% above control level. Growth hormone release inhibiting hormone (GH-RIH) (somatostatin) showed significant effects only in the 20 h incubation; GH release was inhibited by 50% and release of PRL was slightly, but significantly, enhanced. Pituitary cell monolayers apparently permit maximal expression of releasing activities inherent in the hypothalamic hormones.

Pituitary failure secondary to head trauma

1976 ◽  
Vol 44 (4) ◽  
pp. 504-505 ◽  
Author(s):  
William W. Winternitz ◽  
James A. Dzur
Keyword(s):  
Growth Hormone ◽  
Head Trauma ◽  
Anterior Pituitary ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
Severe Head Trauma ◽  
Content Type ◽  
Hypothalamic Control ◽  
Fine Print ◽  
Stimulating Hormone

✓ A patient presented with hypopituitarism, 2 years after severe head trauma. Deficits of growth hormone, follicle-stimulating hormone, luteinizing hormone, and borderline thyroid-stimulating hormone (TSH) were demonstrated. Normal TSH-releasing hormone (TRH) response and elevated prolactin indicated viable anterior pituitary tissue with inadequate hypothalamic control. Precautions are suggested for recognition and treatment of this syndrome.

scholarly journals Case Report: A Case of Pituitary Adenoma Producing Growth Hormone and Thyroid-Stimulating Hormone Simultaneously

2021 ◽  
Vol 12 ◽  
Author(s):  
Junpei Sanada ◽  
Fuminori Tatsumi ◽  
Shinji Kamei ◽  
Yoshiro Fushimi ◽  
Masashi Shimoda ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Pituitary Adenoma ◽  
Thyroid Function ◽  
Tumor Resection ◽  
Brain Magnetic Resonance Imaging ◽  
Thyroid Stimulating Hormone ◽  
Clinical Findings ◽  
Additional Treatment ◽  
Pituitary Fossa ◽  
Stimulating Hormone

BackgroundPituitary adenoma producing growth hormone (GH) or thyroid-stimulating hormone (TSH) is characterized by various specific symptoms and/or findings. However, the frequency of pituitary adenoma producing both hormones is relatively low. In this report, we show a case of pituitary adenoma producing both GH and TSH simultaneously.Case presentationA 27-year-old woman was diagnosed as acromegaly based on various symptoms and clinical findings. For further examination and treatment, she was hospitalized in our institution. It was likely that this subject had pituitary adenoma producing both GH and TSH. In brain magnetic resonance imaging, there was a giant tumor around pituitary fossa. After the diagnosis of GH- and TSH-producing pituitary adenoma, pituitary tumor resection and cyber knife therapy were performed. In addition, we started additional treatment with somatostatin analog and GH receptor antagonist. After then, GH and insulin-like growth factor (IGF-1) levels were suppressed. After the operation, since thyroid function was not sufficiently suppressed, we started anti-thyroid drug thiamazole. After then, thyroid function was normalized and we stopped thiamazole. In GH and TSH staining, many GH-positive and TSH-positive cells were observed. These findings further confirmed our diagnosis that the pituitary adenoma in this subject produced both GH and TSH simultaneously.ConclusionsWe should bear in mind the possibility of pituitary adenoma producing both GH and TSH at the same time.

Regarding the validity of the endpoint response of the mouse (McKenzie) bioassay for thyrotrophin (TSH)

1981 ◽  
Vol 96 (3) ◽  
pp. 342-349 ◽  
Author(s):  
Melvin Ching
Keyword(s):  
Growth Hormone ◽  
Chemical Composition ◽  
Thyroid Hormone ◽  
Luteinizing Hormone ◽  
Pituitary Hormones ◽  
Thyroid Stimulating Hormone ◽  
Quantitative Estimates ◽  
Rat Pituitary ◽  
Stimulating Hormone

Abstract. The release of radiolabelled thyroid hormone into the circulation in low iodine fed mice has been used extensively as a bioassay for thyroid stimulating hormone (TSH). However, the specificity of several bioassays of pituitary hormones have been subject to question. Consequently, the validity of the assay endpoint for TSH in the mouse was re-evaluated with respect to the effect of luteinizing hormone (LH) whose chemical composition closely resembles that of TSH. Mice, prepared for bioassay of TSH received injections of purified LH or α or β subunits of LH. Identical doses of LH and LH subunits were quantified by LH and TSH radioimmunoassays and the results compared with those obtained by the bioassay. Microgram quantities of LH and subunits of LH elicited appreciable responses in the TSH bioassay but produced only negligible effects in the TSH radioimmunoassay. The response of the TSH bioassay of LH and α or β subunits of LH was 40–56% that obtained with LH radioimmunoassay. However, the pituitary concentrations obtained by TSH bioassay when compared with those obtained by radioimmunoassays for TSH, LH, or growth hormone (GH) paralleled closely the TSH radioimmunoassay data, although in terms of quantitative estimates, there was a 15-fold discrepancy between the TSH assays. Estimations of pituitary concentrations of LH lead to the conclusion that, at the doses normally employed, most crude rat pituitary extracts do not contain sufficient quantities of LH to alter significantly bioassayable (McKenzie) estimates of TSH.

ELECTROPHORETIC SEPARATION OF HORMONES ASSOCIATED WITH SECRETORY GRANULES FROM RAT ANTERIOR PITUITARY GLANDS

1970 ◽  
Vol 63 (2) ◽  
pp. 378-384 ◽  
Author(s):  
D. R. Hodges ◽  
W. H. McShan
Keyword(s):  
Growth Hormone ◽  
Anterior Pituitary ◽  
Secretory Granules ◽  
Follicle Stimulating Hormone ◽  
Pituitary Hormones ◽  
Thyroid Stimulating Hormone ◽  
Disc Electrophoresis ◽  
Hormone Activity ◽  
Pituitary Glands ◽  
Stimulating Hormone

ABSTRACT Electrophoretic analyses of rat, mouse, human and cow anterior pituitary homogenates with subsequent bioassays for hormonal activity have been reported. Comparison of the behaviour of the hormonal activities from rat anterior pituitary secretory granules and that reported for pituitary homogenates was made following disc electrophoresis on polyacrylamide gels. Bioassays of gel segments for the six anterior pituitary hormones resulted in the localization of the activities of five of the six hormones. ACTH activity was not detected. Growth hormone and prolactin were associated with the major cathodal and anodal discs respectively. Luteinizing hormone and thyroid stimulating hormone activities had similar mobilities and were located in a zone just above growth hormone. The activity was not restricted to a discrete, stainable disc in either case. Follicle stimulating hormone activity was detected in a narrow segment containing only one disc a few millimeters below growth hormone. Comparison of the mobilities of the hormones from homogenates and secretory granule extracts suggests that they have essentially similar electrophoretic characteristics at basis pH.

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