Collagen solubility and tensile properties of the rat uterine cervix in late pregnancy: effects of arachidonic acid and prostaglandin F2α

1982 ◽  
Vol 95 (3) ◽  
pp. 341-347 ◽  
Author(s):  
Keith Hillier ◽  
R. M. Wallis
Keyword(s):  
Acetic Acid ◽  
Arachidonic Acid ◽  
Tensile Properties ◽  
Uterine Cervix ◽  
Prostaglandin F2α ◽  
Late Pregnancy ◽  
Collagen Structure ◽  
Collagen Concentration ◽  
Pregnant Animal ◽  
Prostaglandin F

The collagen concentration in rat uterine cervix was less on day 18 of pregnancy than in the non-pregnant animal but did not diminish further as pregnancy proceeded. The solubility of cervical collagen in warm acetic acid (0·5 mol/l) was increased on day 22 compared with days 19, 20 and 21 of pregnancy, and there was a positive correlation of increasing solubility with the tissue rate of creep (a measure of reducing stiffness of the cervix). Treatment of rats subcutaneously with arachidonic acid or prostaglandin F2α (PGF2α) on day 18 of pregnancy decreased the stiffness of the tissue when assessed on day 19 and this was accompanied by increased solubility in cold saline (0·45 mol/l), cold acetic acid and warm acetic acid and a reduction in collagen concentration. These results suggest that collagen properties rather than concentration are important in determining the stiffness of the rat uterine cervix at term and that exogenous PGF2αand arachidonic acid cause biochemical changes in collagen structure unlike those seen at term in untreated animals.

THE CONCENTRATION OF PROSTAGLANDIN F2α IN MATERNAL PLASMA, FOETAL PLASMA AND AMNIOTIC FLUID DURING PREGNANCY IN WOMEN

1975 ◽  
Vol 79 (3) ◽  
pp. 589-597 ◽  
Author(s):  
D. A. Johnson ◽  
P. A. Manning ◽  
J. F. Hennam ◽  
J. R. Newton ◽  
W. P. Collins
Keyword(s):  
Amniotic Fluid ◽  
Prostaglandin F2α ◽  
Late Pregnancy ◽  
Maternal Plasma ◽  
Uterine Contractions ◽  
Prostaglandin F ◽  
Serial Samples ◽  
Considerable Individual Variation ◽  
Venous Plasma

ABSTRACT The concentration of prostaglandin F2α has been determined in serial samples of peripheral venous plasma from women at defined times during labour, and studied in detail throughout two consecutive uterine contractions. In addition, the same compound has been measured in single samples of uterine venous plasma, cord venous plasma, and amniotic fluid in groups of patients during early and late pregnancy, labour and at delivery of the baby. The results from the analysis of peripheral venous plasma show that there is considerable individual variation in the concentration of prostaglandin F2α during labour (mean ± sd, 33.1 ± 11.6 pg/ml). However, it is not possible to establish a definite correlation with either the latent or accelerated phases or with the time of delivery. Furthermore, there is no apparent temporal relationship between the concentrations in peripheral venous plasma and the contractile state of the uterus as assessed by external tocography. In early pregnancy (16th to 20th week) the concentration of prostaglandin F2α (pg/ml, mean ± sd) in peripheral venous plasma is 26.3 ± 4.3 and in amniotic fluid 32.7 ± 26.5. At the 36th week to the start of labour the corresponding values are 27.1 ± 8.1 and 110.0 ± 73.8. At the same time the levels in cord plasma and uterine venous plasma are 100.4 ± 74.9 and 87.9 ± 55.0 respectively. During labour there is a significant increase (P < 0.005, Student's t-test) in the concentration in amniotic fluid (335.1 ± 171.0). The results are discussed in relation to the possible role of prostaglandin F2α in the process of parturition.

scholarly journals Leishmania braziliensis prostaglandin F2α synthase impacts host infection

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Eliza Vanessa Carneiro Alves-Ferreira ◽  
Tiago Rodrigues Ferreira ◽  
Pegine Walrad ◽  
Paul M. Kaye ◽  
Angela Kaysel Cruz
Keyword(s):  
Bone Marrow ◽  
Arachidonic Acid ◽  
Host Cell ◽  
Prostaglandin F2α ◽  
Time Lapse ◽  
Lipid Mediators ◽  
Leishmania Braziliensis ◽  
Macrophage Infection ◽  
Prostaglandin F ◽  
Host Parasite

Abstract Background Prostaglandins (PG) are lipid mediators derived from arachidonic acid metabolism. They are involved in cellular processes such as inflammation and tissue homeostasis. PG production is not restricted to multicellular organisms. Trypanosomatids also synthesize several metabolites of arachidonic acid. Nevertheless, their biological role in these early-branching parasites and their role in host-parasite interaction are not well elucidated. Prostaglandin F2α synthase (PGF2S) has been observed in the Leishmania braziliensis secreted proteome and in L. donovani extracellular vesicles. Furthermore, we previously reported a positive correlation between L. braziliensis PGF2S (LbrPGF2S) expression and pathogenicity in mice. Methods LbrPGF2S gene expression and PGF2α synthesis in promastigotes were detected and quantified by western blotting and EIA assay kit, respectively. To investigate LbrPGF2S localization in amastigotes during bone marrow-derived macrophage infection, parasites expressing mCherry-LbrPGF2S were generated and followed by time-lapse imaging for 48 h post-infection. PGF2S homolog sequences from Leishmania and humans were analyzed in silico using ClustalW on Geneious v6 and EMBOSS Needle. Results Leishmania braziliensis promastigotes synthesize prostaglandin F2α in the presence of arachidonic acid, with peak production in the stationary growth phase under heat stress. LbrPGF2S is a cytoplasmic protein enriched in the secretory site of the parasite cell body, the flagellar pocket. It is an enzyme constitutively expressed throughout promastigote development, but overexpression of LbrPGF2S leads to an increase of infectivity in vitro. The data suggest that LbrPGF2S may be released from intracellular amastigotes into the cytoplasm of bone marrow-derived macrophages over a 48-hour infection period, using time-lapse microscopy and mCherry-PGF2S (mChPGF2S)-expressing parasites. Conclusions LbrPGF2S, a parasite-derived protein, is targeted to the host cell cytoplasm. The putative transfer of this enzyme, involved in pro-inflammatory lipid mediator synthesis, to the host cell suggests a potential role in host-parasite interaction and may partially explain the increased pathogenicity associated with overexpression of LbrPGF2S in L. braziliensis. Our data provide valuable insights to help understand the importance of parasite-derived lipid mediators in pathogenesis.

Potential role for lipoxygenase products of arachidonic acid in prostaglandin F2α-stimulated oxytocin release from the ovine corpus luteum

1994 ◽  
Vol 142 (1) ◽  
pp. 47-52 ◽  
Author(s):  
R G Cooke ◽  
N Ahmad
Keyword(s):  
Arachidonic Acid ◽  
Corpus Luteum ◽  
Potential Role ◽  
Prostaglandin F2α ◽  
Nordihydroguaiaretic Acid ◽  
Luteal Function ◽  
Lipoxygenase Inhibition ◽  
Lipoxygenase Products ◽  
Oxytocin Release ◽  
Prostaglandin F

Abstract Intrauterine administration of nordihydroguaiaretic acid (NDGA) will maintain luteal function in sheep and also suppress the release of both oxytocin and prostaglandin F2α (PGF2α) suggesting that 5-lipoxygenase products of arachidonic acid may be involved in ovine luteolysis. During luteolysis, uterine PGF2α is considered to be the major stimulus for the secretion of luteal oxytocin, and we report the effects of 5-lipoxygenase inhibition, via intrauterine NDGA administration, on the ability of PGF2α to effect such secretion. In the NDGA-treated ewes, luteal function was maintained and oestrus delayed, the duration of the oestrous cycle (20 ±1 days; mean ± s.d.; n=9) being significantly (P<0·01) longer than in intact controls (15 ± 1 days, n=4). Jugular infusions of PGF2α did not stimulate luteal secretion of oxytocin, the effects being comparable with those in ovariectomized ewes. In intact ewes receiving intrauterine infusions of vehicle only, PGF2α produced marked increases in luteal secretion of oxytocin. Also, preinfusion or basal concentrations of oxytocin in this group of ewes (6·6 ± 1·9 pg/ml) were significantly (P<0·01) greater than in either the NDGA-treated (3·1 ± 1·1 pg/ml) or ovariectomized (3·0 ± 0·6 pg/ml) ewes. The results suggest involvement of 5-lipoxygenase products of arachidonic acid in the release of oxytocin from the ovine corpus luteum. Journal of Endocrinology (1994) 142, 47–52

Expression of prostaglandin G/H synthase (PGHS) and heat shock protein-70 (HSP-70) in the corpus luteum (CL) of prostaglandin F2α-treated immature superovulated rats

2004 ◽  
Vol 82 (6) ◽  
pp. 363-371 ◽  
Author(s):  
R M Narayansingh ◽  
M Senchyna ◽  
M M Vijayan ◽  
J C Carlson
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Corpus Luteum ◽  
Heat Shock Protein 70 ◽  
Prostaglandin F2α ◽  
Sampling Period ◽  
Immunoblot Analysis ◽  
Hsp 70 ◽  
Prostaglandin F

In this study we examined the mechanism of corpus luteum (CL) regression by measuring changes in expression of prostaglandin G/H synthase-1 (PGHS-1) and -2 (PGHS-2) in day 4 CL and inducible heat shock protein 70 (HSP-70) in day 4 and day 9 CL of immature superovulated rats. The rats were superovulated and treated with 500 µg of prostaglandin F2α (PGF2α) on day 4 or day 9 after CL formation. Ovaries and serial blood samples were removed during the 24-hour period following treatment. Plasma progesterone was determined by radioimmunoassay while mRNA abundance and protein expression were assessed by semiquantitative RT-PCR and immunoblot analysis, respectively. One hour after PGF2α, both day 4 and day 9 rats exhibited a significant decrease in progesterone secretion; however, there was a greater decrease in day 9 rats. In ovarian samples removed on day 4, there was a significant increase in mRNA for PGHS-2 at 1 hour after PGF2α. PGHS-1 mRNA content remained unchanged. Immunoblot analyses showed an increase in PGHS-2 protein expression only at 8 h. There were no changes in PGHS-1 protein expression. In day 9 rats, ovarian HSP-70 protein levels increased by 50% after PGF2α injection; however, on day 4 there was no change in expression of this protein over the sampling period. These results suggest that expression of PGHS-2 may be involved in inhibiting progesterone production and that expression of HSP-70 may be required for complete CL regression in the rat.Key words: rat, prostaglandin F2α, corpus luteum, prostaglandin G/H synthase, heat shock protein-70.

Comparative Analysis of the Proteomic Profile of Cattle Hides that Produce Loose and Tight Leather using In-Gel Tryptic Digestion followed by LC-MS/MS

2020 ◽  
Vol 115 (11) ◽  
pp. 399-408
Author(s):  
Catherine Maidment ◽  
Meekyung Ahn ◽  
Rafea Naffa ◽  
Trevor Loo ◽  
Gillian Norris
Keyword(s):  
Type I Collagen ◽  
Raw Material ◽  
Type I ◽  
Collagen Structure ◽  
Proteomic Profile ◽  
Collagen Network ◽  
Collagen Concentration ◽  
Different Types ◽  
Molecular Components ◽  
First Time

Looseness is a defect found in leather that reduces its quality by causing a wrinkly appearance in the finished product, resulting in a reduction in its value. Earlier studies on loose leather using microscopy and Raman spectroscopy reported a change in the collagen structure of loose leather. In this study, proteomics was used to investigate the possible molecular causes of looseness in the raw material, the first time such a study has been carried out. Proteins extracted from two regions of raw hide using two different methods were analysed; those taken from the distal axilla, an area prone to looseness, and those taken from the backbone which is less prone to looseness. Analyses using 1DE-LC-MS/MS showed that although the overall collagen concentration was similar in both areas of the hide, the distribution of the different types of collagen differed.  Specifically, concentrations of type I collagen, and the collagen-associated proteoglycan decorin were lower in samples taken from the distal axilla, symptomatic of a collagen network with excess space seen for these samples using confocal microscopy. This study suggests a possible link between the molecular components of raw cattle hide and looseness and more importantly between the molecular components of skin and skin defects. There is therefore potential to develop biomarkers for looseness which will enable early preventative action.

scholarly journals The oophorectomy effect on Walker 256 tumor inoculated into the vagina and uterine cervix of female rats

2009 ◽  
Vol 24 (1) ◽  
pp. 26-29 ◽  
Author(s):  
Nara Macedo Botelho Brito ◽  
Rita de Kássia Vidigal Carvalho ◽  
Lia Tavares de Moura Brasil Matos ◽  
Rodolfo Costa Lobato ◽  
Rosângela Baía Brito
Keyword(s):  
Acetic Acid ◽  
Tumor Growth ◽  
Uterine Cervix ◽  
Statistical Difference ◽  
Female Rats ◽  
Tumor Mass ◽  
Tumor Group ◽  
Walker 256 ◽  
Similar Development ◽  
Walker 256 Tumor

PURPOSE: Verify the effect of oophorectomy on the evolution of the Walker 256 tumor inoculated into the vagina and cervix of female rats. METHODS: Ten Wistar, female rats were used, distributed into two groups with 05 animals each: Tumor group (TG): Rats inoculated with Walker 256 tumor; Oophorectomy group (OG): oophorectomized rats inoculated with Walker 256 tumor. The day before the tumor vaginal inoculation, acetic acid was inoculated into the vaginas of both groups of rats; the following day, the vaginal walls were scarified with an endocervix brush, and then Walker 256 tumor was inoculated. After 12 days, the tumor was removed together with the vagina and uterine horns for macro and microscopic analyses. The data were submitted to statistical analyses. RESULTS: There was no statistical difference between the two groups; however it was observed that the behavior of tumor growth on the OG group presented greater invasion, compromising the uterine horns. CONCLUSION: The results of the study on the GO group presented a macroscopic behavior different from the TG group, however, both of them presented similar development in terms of tumor mass.

EFFECTS OF PROSTAGLANDIN F2α ON SOME REPRODUCTIVE PROCESSES OF HAMSTERS AND RATS

1972 ◽  
Vol 53 (2) ◽  
pp. 201-213 ◽  
Author(s):  
A. P. LABHSETWAR
Keyword(s):  
Prostaglandin F2α ◽  
Concomitant Administration ◽  
Corpora Lutea ◽  
Fallopian Tubes ◽  
Racemic Form ◽  
Endocrine Profile ◽  
Prostaglandin F ◽  
Blue Reaction ◽  
Egg Transport ◽  
Reproductive Processes

SUMMARY In an attempt to characterize the endocrine profile of prostaglandin F2α (PGF2α) in relation to the female reproductive system, the compound (racemic form) was administered to hamsters and rats in various reproductive states. The prostaglandin terminated pregnancy when given once a day either subcutaneously (50 μg/hamster) or orally (1·5–2 mg/hamster) from Days 4 to 6 of pregnancy inclusive, or as a single subcutaneous injection (50 μg/animal) on Day 4. In the rat, higher (500 μg/injection) and more frequent (twice daily) s.c. injections were required to get even foetal resorption. Concomitant administration of progesterone (4 mg/animal) in either species protected pregnancy. Prostaglandin F2α terminated pregnancy without interfering with the Pontamine blue reaction, suggesting that its antifertility effects were not mediated by inhibition of implantation. In both hamsters and rats the prostaglandin markedly reduced the size of deciduomata which could be restored to normal by administration of progesterone. Prostaglandin F2α delayed passage of zygotes through the Fallopian tubes in a proportion of rats but failed to accelerate egg transport in rats and hamsters. Furthermore, it caused a marked histological degeneration of the corpora lutea and induced formation of a fresh set of corpora lutea in pseudopregnant, pregnant and pseudopregnant—hysterectomized hamsters. These deleterious effects of prostaglandin were accompanied, in hamsters, by the appearance of freshly ovulated tubal ova. Most of the endocrine effects of PGF2α observed in this study can be accounted for by its luteolytic property.

INHIBITION OF OXYTOCIN- OR PROSTAGLANDIN F2α-DRIVEN MYOMETRIAL ACTIVITY BY RELAXIN IN THE RAT IS OESTROGEN-DEPENDENT

1981 ◽  
Vol 89 (3) ◽  
pp. 399-404 ◽  
Author(s):  
D. G. PORTER ◽  
SANDRA J. DOWNING ◽  
JANE M. C. BRADSHAW
Keyword(s):  
Smooth Muscle ◽  
Prostaglandin F2α ◽  
Ovariectomized Rats ◽  
Inhibitory Effects ◽  
Arterial Infusion ◽  
Uterine Smooth Muscle ◽  
Small Doses ◽  
Prostaglandin F

Relaxin in doses of 5 μg i.v. completely but reversibly abolishes 'spontaneous' myometrial activity in anaesthetized ovariectomized rats. Similar levels of myometrial activity, evoked in oestrogen-treated rats (which normally have quiescent uteri) by infusions of oxytocin or prostaglandin F2α (PGF2α), were also reduced to complete quiescence by relaxin in small doses. However when spontaneous myometrial activity in untreated ovariectomized rats was slightly stimulated by oxytocin the uterus became completely refractory to the inhibitory effects of relaxin even at doses of 50 μg. Relaxin was also ineffective in reducing myometrial activity in similar rats during intra-arterial infusion of PGF2α. It is suggested that the ability of relaxin to inhibit uterine smooth muscle during exogenous stimulation is oestrogen-dependent.

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