scholarly journals Secretory pattern of inhibin A, inhibin B and inhibin pro-alpha C during induced follicular atresia and subsequent follicular development in the golden hamster (Mesocricetus auratus)

2002 ◽  
Vol 172 (3) ◽  
pp. 575-581 ◽  
Author(s):  
K Ohshima ◽  
H Kishi ◽  
M Itoh ◽  
KY Arai ◽  
G Watanabe ◽  
...  
Keyword(s):  
Golden Hamster ◽  
Plasma Concentrations ◽  
Follicular Development ◽  
Inhibin B ◽  
Follicular Atresia ◽  
Inhibin A ◽  
Antral Follicles ◽  
Initial Values ◽  
Time Courses ◽  
Secretory Pattern

The changes in plasma concentrations of inhibins A, B and pro-alpha C were determined in the cyclic golden hamster during follicular atresia induced with antiserum against luteinizing hormone releasing hormone (LHRH-AS) at 1100 h on day 4 (day 1=day of ovulation). Follicular status in the ovary was also studied by determining the number of follicles ovulating in response to human chorionic gonadotrophin (hCG) injection. The time-courses of changes in plasma concentrations of inhibins A, B and pro-alpha C were different from each other during induced follicular atresia and subsequent follicular development. Plasma concentrations of inhibin A decreased to 58.6% of initial values by 24 h after LHRH-AS treatment, and then remained relatively low until at least 60 h later. Plasma concentrations of inhibin B decreased to 64.2% of the initial values by 18 h after LHRH-AS treatment and remained at basal values for 36 h, but increased abruptly to greater than initial values at 42 h after the treatment. Plasma concentrations of inhibin pro-alpha C increased at 6 and 12 h, decreased suddenly to 21.9% of the initial values by 24 h after LHRH-AS treatment, and then gradually increased until 60 h after LHRH-AS. The number of follicles responding to hCG decreased gradually between 0 and 30 h after LHRH-AS, when no ovulations were observed, and then gradually increased until 60 h. The changes in follicular ovulatory responses to hCG correlated with the plasma profile of inhibin A throughout the experiment. These results suggest that inhibin A is mainly secreted by large antral follicles. In contrast, during the subsequent follicular development, the plasma concentration of inhibin B increased earlier than that of inhibin A. These results suggest that inhibin B is secreted by small and large antral follicles. Plasma concentrations of inhibin pro-alpha C were high at a time when plasma concentrations of oestradiol-17 beta had already decreased, indicating that inhibin pro-alpha C is secreted not only from healthy follicles but also from early atretic antral follicles.

scholarly journals Secretion of inhibin A, inhibin B and inhibin pro-alphaC during the oestrous cycle of the golden hamster (Mesocricetus auratus)

1999 ◽  
Vol 162 (3) ◽  
pp. 451-456 ◽  
Author(s):  
K Ohshima ◽  
H Kishi ◽  
M Itoh ◽  
G Watanabe ◽  
K Arai ◽  
...  
Keyword(s):  
Golden Hamster ◽  
Plasma Concentrations ◽  
Follicular Development ◽  
Early Morning ◽  
Oestrous Cycle ◽  
Inhibin B ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inhibin A ◽  
Negative Relationships ◽  
Plasma Oestradiol

Plasma concentrations of inhibin pro-alphaC, inhibin A and inhibin B were determined by enzyme-linked immunosorbent assay at 6 h intervals throughout the 4-day oestrous cycle of the golden hamster. Plasma concentrations of follicle-stimulating hormone (FSH) and oestradiol-17beta were also measured by radioimmunoassay during the oestrous cycle. Plasma concentrations of inhibin A increased from the early morning of day 1 (day 1=day of ovulation) and reached plateau levels at 0500 h on day 2. An abrupt increase in plasma concentrations of inhibin A was found at 1700 h on day 4, when the preovulatory FSH surge was observed. An increase in plasma concentrations of inhibin B occurred on day 1 and reached plateau levels at 1700 h on day 1. The levels remained elevated until 0500 h on day 4 and declined gradually by 2300 h on day 4. Plasma concentrations of inhibin pro-alphaC gradually increased with some fluctuation from day 1 to 1700 h on day 4 and then declined. Significant negative relationships were noted between plasma FSH and both dimeric forms of inhibin from day 1 to day 3. Significant positive relationships were found between plasma oestradiol-17beta and inhibin A or inhibin pro-alphaC throughout the oestrous cycle. In contrast, no significant relationship was found between plasma oestradiol-17beta and inhibin B. These findings suggest that both dimeric forms of inhibin play a role in the regulation of FSH secretion during follicular development. These findings also suggest that inhibin pro-alphaC could be secreted primarily by large follicles, and early atretic follicles could also be responsible for inhibin pro-alphaC secretion. On the other hand, the secretory pattern of dimeric inhibins might shift from inhibin B to inhibin A with follicular development.

scholarly journals Potential role of activin A in follicular development during the second half of pregnancy in the golden hamster: utero-placental source of activin A

2002 ◽  
Vol 172 (2) ◽  
pp. 247-253 ◽  
Author(s):  
K Ohshima ◽  
K Ohshima ◽  
KY Arai ◽  
H Kishi ◽  
M Itoh ◽  
...  
Keyword(s):  
Golden Hamster ◽  
Potential Role ◽  
Follicular Development ◽  
Activin A ◽  
Inhibin A ◽  
Abrupt Decrease ◽  
Antral Follicles ◽  
Insight Into

Numerous antral follicles develop during the second half of pregnancy in the golden hamster. However, mechanisms regulating follicular development during this period are unknown. Because inhibin and activin are related to follicular development, these hormones were studied to gain insight into any potential roles in follicular development. Plasma inhibin A and B suddenly increased from day 8 of pregnancy, reached peak levels on day 10 and gradually declined to term. Plasma activin A gradually increased from day 8 to day 15 of pregnancy, and this was followed by an abrupt decrease at day one of lactation. Ovariectomy on day 12 of pregnancy rapidly reduced plasma inhibin A and B, but not activin A levels. Hysterectomy or placentectomy on day 12 of pregnancy caused an abrupt decrease in the levels of plasma activin A and FSH, but not inhibin A and B at 6 h after surgery. Hysterectomy also induced atresia of large antral follicles at 24 h after surgery. These results indicate that antral follicles are the main source of circulating inhibin A and B, whereas uteri and placentae are the main source of circulating activin A. These results suggest that increased levels of activin A may be involved in folliculogenesis in the ovary during the second half of pregnancy in the golden hamster.

Follicular dynamics and secretion of inhibin and oestradiol-17β during the oestrous cycle of the hamster

1995 ◽  
Vol 146 (1) ◽  
pp. 169-176 ◽  
Author(s):  
H Kishi ◽  
K Taya ◽  
G Watanabe ◽  
S Sasamoto
Keyword(s):  
Plasma Levels ◽  
Marked Decrease ◽  
Plasma Concentrations ◽  
Follicular Development ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Oestrous Cycle ◽  
Antral Follicles ◽  
Follicular Maturation ◽  
Follicular Dynamics

Abstract Plasma and ovarian levels of inhibin were determined by a radioimmunoassay (RIA) at 3-h intervals throughout the 4-day oestrous cycle of hamsters. Plasma concentrations of FSH, LH, progesterone, testosterone and oestradiol-17β were also determined by RIAs. In addition, hamsters were injected at various times with human chorionic gonadotrophin (hCG) to determine the follicular development. The changes in plasma concentrations of FSH after injection of antisera to oestradiol-17β (oestradiol-AS) and inhibin (inhibin-AS) on the morning of day 2 (day 1=day of ovulation) were also determined. Plasma concentrations of inhibin showed a marked increase on the afternoon of day 1, remained at plateau levels until the morning of day 4, then increased abruptly on the afternoon of day 4 when preovulatory LH and FSH surges were initiated. A marked decrease in plasma concentrations of inhibin occurred during the process of ovulation after the preovulatory gonadotrophin surges. An inverse relationship between plasma levels of FSH and inhibin was observed when the secondary surge of FSH was in progress during the periovulatory period. Plasma concentrations of oestradiol-17β showed three increase phases and these changes differed from those of inhibin. Changes in plasma concentrations of oestradiol-17β correlated well with the maturation and regression of large antral follicles. Follicles capable of ovulating following hCG administration were first noted at 2300 h on day 1. The number of follicles capable of ovulating reached a maximum on the morning of day 3 (24·8± 0·6), and decreased by 0500 h on day 4 (15·0 ± 1·1), corresponding to the number of normal spontaneous ovulations. Plasma concentrations of FSH were dramatically increased within 6 h after inhibin-AS, though no increase in FSH levels was observed after oestradiol-AS. These findings suggest that changes in the plasma levels of inhibin during the oestrous cycle provide a precise indicator of follicular recruitment, and that the changes in plasma concentrations of oestradiol-17β are associated with follicular maturation. These findings also suggest that inhibin may play a major role in the inhibition of FSH secretion during the oestrous cycle of the hamster. Journal of Endocrinology (1995) 146, 169–176

scholarly journals Secretion of inhibin A and inhibin B throughout pregnancy and the early postpartum period in chimpanzees

2001 ◽  
Vol 168 (2) ◽  
pp. 257-262 ◽  
Author(s):  
M Kondo ◽  
M Kondo ◽  
T Udono ◽  
WZ Jin ◽  
WZ Jin ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Postpartum Period ◽  
Plasma Concentrations ◽  
Late Pregnancy ◽  
Inhibin B ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inhibin A ◽  
Early Postpartum Period ◽  
Early Postpartum ◽  
Normal Menstrual Cycle

Plasma concentrations of inhibin A and inhibin B during pregnancy and early lactation in chimpanzees were determined by enzyme-linked immunosorbent assay (ELISA). Plasma samples were taken from five pregnant chimpanzees at 6-9, 10, 20 and 25 weeks of pregnancy, and following parturition. Throughout pregnancy and the early postpartum period, circulating inhibin A and inhibin B concentrations remained low, at similar levels to those during the normal menstrual cycle in chimpanzees. Concentrations of inhibin A in the placental homogenate were high enough to be measured by the ELISA and by bioassay, whereas circulating inhibin bioactivities in late pregnancy were too low to be measured. Plasma concentrations of FSH remained low with no significant changes throughout pregnancy and the postpartum period. Plasma concentrations of oestradiol-17beta and progesterone at 25 weeks of pregnancy were much higher than normal menstrual cycle levels. It was concluded that in chimpanzees the levels of circulating inhibin A and inhibin B remained low throughout pregnancy and the early postpartum period, and that the concentrations of bioactive dimeric inhibin did not increase towards the end of pregnancy. The suppression of circulating FSH levels during pregnancy is suggested to be controlled by steroid hormones that increased significantly in late pregnancy, and the present findings further suggest that the secretory pattern and role of inhibin during pregnancy in chimpanzees may be different from that in human and other primates.

INITIATION OF FOLLICULAR MATURATION AND OVULATION AFTER REMOVAL OF THE LITTER FROM THE LACTATING RAT

1980 ◽  
Vol 87 (3) ◽  
pp. 393-400 ◽  
Author(s):  
KAZUYOSHI TAYA ◽  
SHUJI SASAMOTO
Keyword(s):  
Plasma Levels ◽  
Plasma Concentrations ◽  
Follicular Development ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Moderate Increase ◽  
Normal Cycle ◽  
Antral Follicles ◽  
Follicular Maturation ◽  
Follicular Activity

In order to elucidate the mechanism of the resumption of follicular activity and ovulation in rats, levels of FSH, LH and prolactin in plasma and pituitary gland and ovarian follicular development were quantified after removal of the litter on day 3 of lactation (day of parturition = day 0 of lactation). Such removal resulted in ovulation of 13 oocytes 4 days later, a number comparable with that found in normal cyclic rats. Plasma levels of prolactin were high during lactation but markedly decreased after removal of the litter. Although plasma concentrations of FSH and LH did not change during days 3–7 of lactation, there was an FSH surge between 24 and 30 h after removal of the litter. Plasma concentrations of LH also increased slightly but significantly by 24 h after removal of the litter and this value persisted during the following 2 days. Surges of FSH, LH and prolactin occurred at 17.00 h 3 days after pups were removed. Removal of the litter did not increase pituitary contents of FSH, LH and prolactin and a marked reduction in pituitary levels of FSH and LH, but not of prolactin, occurred at 17 00 h 3 days after removal of the litter. A quantitative study of follicular development indicated that follicles larger than 401 μm in diameter were absent during days 3–7 of lactation. However, the number and size of antral follicles increased by 30 h after removal of the litter, probably due to the increases in plasma levels of FSH and LH, and follicles larger than 601 μm in diameter appeared 3 days after the young were removed. Although ovulation could not be induced by human chorionic gonadotrophin from days 3 to 5 of lactation, its administration 30 h after removal of the litter produced ovulation in all rats by the following morning. These results indicated that a moderate increase in FSH, although below the amounts released at the preovulatory surge, together with basal levels of LH which were within the range observed on the day of dioestrus during the normal cycle were responsible for the initiation of follicular maturation after removal of the litter.

scholarly journals Peripheral concentrations of inhibin A, ovarian steroids, and gonadotropins associated with follicular development throughout the estrous cycle of the sow

Reproduction ◽  
2010 ◽  
Vol 139 (1) ◽  
pp. 153-161 ◽  
Author(s):  
Michiko Noguchi ◽  
Koji Yoshioka ◽  
Seigo Itoh ◽  
Chie Suzuki ◽  
Sachiko Arai ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Luteal Phase ◽  
Inverse Relationship ◽  
Plasma Concentrations ◽  
Follicular Development ◽  
Follicular Phase ◽  
Follicular Growth ◽  
Inhibin A ◽  
Ovarian Steroids ◽  
Feedback Regulator

We investigated changes in peripheral concentrations of inhibin A, total inhibin, steroids, and gonadotropins throughout the intact estrous cycle of the sow in relation to ovarian changes determined by daily transrectal ultrasonography. All visible follicles of 3 mm or more in diameter were classified as small (≥3 and <6 mm) or large (≥6 mm). Follicular recruitment was identified in two periods of the cycle: one from the late luteal to the follicular phase, characterized by an increase in the number of small follicles followed by the appearance of large follicles; and another during the early luteal phase, consisting only of increased numbers of small follicles. Plasma concentrations of inhibin A increased (P<0.05), coinciding with the two periods of follicle emergence. Estradiol (E2) levels increased (P<0.05) during the follicular phase, but not during the early luteal phase. An inverse relationship (P<0.01) between the patterns of inhibin and FSH concentrations was noted around the two periods of follicle emergence, but there was no relationship (P≥0.1) between the patterns of plasma E2and FSH during the early luteal phase. In conclusion, measurement of plasma inhibin A levels combined with ultrasonographic examination of the ovaries revealed two periods of synchronous follicular growth during the sow's estrous cycle. The results strongly suggest that inhibin A functions as a negative feedback regulator of FSH secretion throughout the estrous cycle, whereas E2appears to influence FSH secretion only during the follicular phase.

scholarly journals Estradiol and Regulation of Anti-Müllerian Hormone, Inhibin-A, and Inhibin-B Secretion: Analysis of Small Antral and Preovulatory Human Follicles’ Fluid

2006 ◽  
Vol 91 (10) ◽  
pp. 4064-4069 ◽  
Author(s):  
Claus Yding Andersen ◽  
Anne Grete Byskov
Keyword(s):  
Outcome Measures ◽  
Negative Correlation ◽  
Significant Negative Correlation ◽  
Inhibin B ◽  
University Hospital ◽  
Cancer Disease ◽  
Inhibin A ◽  
Antral Follicles ◽  
Preovulatory Follicles ◽  
Very High

Abstract Context: In ovaries surgically removed for fertility preservation, hormone concentrations in fluid from small antral follicles were determined. Levels were compared with those found in preovulatory follicular fluid. Objective: The objective of this study is to measure intrafollicular concentrations of anti-Müllerian hormone (AMH), inhibin-A, inhibin-B, estradiol, and progesterone. Setting: The study was set in a university hospital. Patients: Patients were 22 women suffering from a cancer disease and 16 women undergoing assisted reproduction. Interventions: Fluid from 35 follicles (diameter, 3–8 mm) was included and compared with that of 32 preovulatory follicles. Main Outcome Measures: The main outcome measures were intrafollicular concentrations of the measured hormones and their possible correlation. Results: Concentrations of AMH in small antral follicles were almost three orders of magnitude higher than in follicle fluid of preovulatory follicles, 790 ± 95 vs. 1.17 ± 0.14 ng/ml (mean ± sem), respectively. There was a significant negative correlation between estradiol and AMH in fluid from small antral follicles, whereas inhibin-A and inhibin-B were correlated positively with estradiol concentrations. Progesterone showed a similar correlation to levels of AMH but only in fluid of preovulatory follicles. Conclusions: The high expression of AMH in granulosa cells of small antral follicles actually translates into very high follicle fluid AMH concentrations. This most likely explains the correlation between serum AMH levels and the number of small antral follicles as previously demonstrated. The negative correlation between estradiol and AMH suggests that FSH down-regulates AMH expression. Thus, the microenvironment of the follicle shows profound changes with developmental stage and highlights the importance of studies to understand the mechanisms that regulate follicular growth and development during antral stages of development.

scholarly journals Follicular waves and concentrations of steroids and inhibin A in ovarian venous blood during the luteal phase of the oestrous cycle in ewes with an ovarian autotransplant

1998 ◽  
Vol 156 (3) ◽  
pp. 563-572 ◽  
Author(s):  
CJ Souza ◽  
BK Campbell ◽  
DT Baird
Keyword(s):  
Luteal Phase ◽  
Venous Blood ◽  
Follicular Development ◽  
Inhibin A ◽  
Ovarian Steroids ◽  
Antral Follicles ◽  
Follicular Wave ◽  
Follicle Diameter ◽  
Before And After ◽  
Follicular Size

The dynamics of ovarian follicular development and the pattern of pituitary and ovarian hormone concentration were investigated during the luteal phase in ewes with autotransplanted ovaries. The follicles were measured by ultrasound and samples of ovarian and jugular venous blood were collected at intervals of 12 h. Blood samples were collected before and after a GnRH challenge (250 ng GnRH, i.v.) to allow the determination of basal and LH-stimulated concentration of ovarian steroids. Throughout the luteal phase, large antral follicles developed in three waves, each of which was preceded by a rise in the concentration of FSH (P < 0.05). The concentrations of oestradiol and androstenedione in the unstimulated and LH-stimulated samples were similar (P > 0.05) during the first 3 days of the luteal phase but differed thereafter, with the LH-stimulated being significantly higher than the basal concentrations (P < 0.05). In the first wave of follicular development the changes in follicular size were accompanied by an increase in the concentration of ovarian steroids and inhibin A. During the second follicular wave, although changes in follicle diameter were similar to the first wave (P > 0.05), the basal concentration of ovarian steroids and inhibin A remained unchanged throughout the period of emergence and demise of the large follicles. These results confirm that the development of large antral follicles during the luteal phase of the sheep occurs in successive waves that are associated with fluctuations in FSH secretion. However while the results strongly suggest that fluctuations in both inhibin A and oestradiol secretion control FSH during the first follicular wave, the cause of the FSH fluctuations associated with waves two and three is unclear. Final resolution of this issue may need to await the development of a specific assay for dimeric inhibin B.

THE EFFECT OF A SINGLE INJECTION OF DIETHYLSTILBOESTROL OR PROGESTERONE ON THE HAMSTER OVARY

1965 ◽  
Vol 33 (1) ◽  
pp. 13-23 ◽  
Author(s):  
G. S. GREENWALD
Keyword(s):  
Anterior Pituitary ◽  
Single Injection ◽  
Follicular Development ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Oestrous Cycle ◽  
The Other ◽  
Follicular Atresia ◽  
Follicular Growth ◽  
Antral Follicles

SUMMARY A single injection of 0·25 mg. stilboestrol or 5 mg. progesterone at metoestrus (day 1) affected follicular development in the hamster ovary in different ways. Stilboestrol induced widespread follicular atresia but apparently did not interfere with the release of ovulating hormone at the end of the oestrous cycle. The atresia produced by stilboestrol appears to be mediated by changes in the levels of circulating gonadotrophin rather than by a direct effect on the ovary. This was demonstrated by injecting pregnant mare serum on day 1 of the cycle followed by stilboestrol treatment at various times thereafter. Under these circumstances the ovulation rate was only reduced below control values when stilboestrol was injected on day 1. Progesterone given on day 1 of the cycle did not interfere with the maturation of healthy Graafian follicles but acted on the terminal stages of follicular growth by blocking ovulation. After a single injection of progesterone, the life span of antral follicles was prolonged to 8–9 days. The ovulation-inhibiting effects of progesterone given on day 1 of the cycle were overcome by the injection of human chorionic gonadotrophin on day 4. Thus, progesterone blocked ovulation indirectly by preventing release of ovulating hormone from the anterior pituitary. The effects of shifting the single injection of stilboestrol or progesterone to the other days of the oestrous cycle are also considered.

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