Burning magnesium, a sparkle in acute inflammation: gleams from experimental models

Rivaroxaban (RVX) was suggested to possess anti-inflammatory and vascular tone modulatory effects. The goal of this study was to investigate whether RVX impacts lipopolysaccharide (LPS)-induced acute vascular inflammatory response. Male rats were treated with 5 mg/kg RVX (oral gavage) followed by 10 mg/kg LPS i.p injection. Circulating levels of IL-6, MCP-1, VCAM-1, and ICAM-1 were measured in plasma 6 and 24 hours after LPS injection, while isolated aorta was used for gene expression analysis, immunohistochemistry, and vascular tone evaluation. RVX pre-treatment significantly reduced LPS mediated increase after 6h and 24h for IL-6 (4.4±2.2 and 2.8±1.7 fold), MCP-1 (1.4±1.5 and 1.3±1.4 fold) VCAM-1 (1.8±2.0 and 1.7±2.1 fold). A similar trend was observed in the aorta for iNOS (5.5±3.3 and 3.3±1.9 folds reduction, P<0.01 and P<0.001, respectively), VCAM-1 (1.3±1.2 and 1.4±1.3 fold reduction, P<0.05), and MCP-1 (3.9±2.2 and 1.9±1.6 fold reduction, P<0.01). Moreover, RVX pre-treatment, improved LPS-induced PE contractile dysfunction in aortic rings (Control vs LPS, Emax reduction = 35.4 and 31.19%, P<0.001; Control vs LPS+RVX, Emax reduction = 10.83 and 11.48%, P>0.05, respectively), resulting in 24.5% and 19.7% change in maximal constriction in LPS and LPS+RVX respectively. These data indicate that RVX pre-treatment attenuates LPS-induced acute vascular inflammation and contractile dysfunction.

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Inhibition of Carrageenan-Induced Acute Inflammation in Mice by Oral Administration of Anthocyanin Mixture from Wild Mulberry and Cyanidin-3-Glucoside

BioMed Research International ◽

10.1155/2013/146716 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 22

Author(s):

Neuza Mariko Aymoto Hassimotto ◽

Vanessa Moreira ◽

Neide Galvão do Nascimento ◽

Pollyana Cristina Maggio de Castro Souto ◽

Catarina Teixeira ◽

...

Keyword(s):

Polymorphonuclear Leukocytes ◽

Acute Inflammation ◽

Biological Activities ◽

Inhibitory Effect ◽

Experimental Models ◽

Protein Levels ◽

Paw Oedema ◽

Cox 2

Anthocyanins are flavonoids which demonstrated biological activities inin vivoandin vitromodels. Here in the anti-inflammatory properties of an anthocyanin-enriched fraction (AF) extracted from wild mulberry and the cyanidin-3-glucoside (C3G), the most abundant anthocyanin in diet, were studied in two acute inflammation experimental models, in the peritonitis and in the paw oedema assays, both of which were induced by carrageenan (cg) in mice. In each trial, AF and C3G (4 mg/100 g/animal) were orally administered in two distinct protocols: 30 min before and 1 h after cg stimulus. The administration of both AF and C3G suppresses the paw oedema in both administration times (P<0.05). In the peritonitis, AF and C3G reduced the polymorphonuclear leukocytes (PMN) influx in the peritoneal exudates when administered 1 h after cg injection. AF was more efficient reducing the PMN when administered 30 min before cg. Both AF and C3G were found to suppress mRNA as well as protein levels of COX-2 upregulated by cg in both protocols, but the inhibitory effect on PGE2production in the peritoneal exudates was observed when administered 30 min before cg (P<0.05). Our findings suggest that AF and C3G minimize acute inflammation and they present positive contributions as dietary supplements.

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Inhibitory Effect ofHelicteres gardnerianaEthanol Extract on Acute Inflammation

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/141947 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

Juliana Oliveira de Melo ◽

Laura Lícia Milani de Arruda ◽

Silmara Baroni ◽

Maria da Conceição Torrado Truiti ◽

Silvana Martins Caparroz-Assef ◽

...

Keyword(s):

Acute Inflammation ◽

Pleural Cavity ◽

Ethanol Extract ◽

Inhibitory Effect ◽

Experimental Models ◽

Perivascular Space ◽

Polymorphonuclear Cells ◽

Anti Inflammatory ◽

Inflammatory Effect

The anti-inflammatory effect of an ethanol extract ofHelicteres gardneriana(Nees) Castiglioni was assayed in experimental models of pleurisy and microcirculationin situ. Treatment of animals with 500 mg/kg body weight reduced the exudate volume (35% reduction) induced by intrapleural injection of carrageenan and the migration of polymorphonuclear cells into the inflamed pleural cavity of rats (40%). Additionally, rolling and adhesion of leukocytes and the number of leukocytes that migrated toward the perivascular space in response to the carrageenan injection were decreased by the extract (500 mg/kg). These data demonstrate the anti-inflammatory effect of the ethanol extract ofHelicteres gardnerianaand imply that inhibition of leukocyte-endothelial interactions is important in the extract's mechanism of action.

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Chemical characterization of a red raspberry fruit extract and evaluation of its pharmacological effects in experimental models of acute inflammation and collagen-induced arthritis

Food & Function ◽

10.1039/c4fo00376d ◽

2014 ◽

Vol 5 (12) ◽

pp. 3241-3251 ◽

Cited By ~ 18

Author(s):

M. E. Figueira ◽

M. B. Câmara ◽

R. Direito ◽

J. Rocha ◽

A. T. Serra ◽

...

Keyword(s):

Wistar Rats ◽

Acute Inflammation ◽

Clinical Signs ◽

Chemical Characterization ◽

Experimental Models ◽

Pharmacological Effects ◽

Red Raspberry ◽

Collagen Induced Arthritis ◽

Fruit Extract

A red raspberry extract reduces inflammation and the development of clinical signs of arthritis in Wistar rats.

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ANTI-INFLAMMATORY EFFECTS OF PROPOXAZEPAM ON DIFFERENT MODELS OF INFLAMMATION

International Journal of Medicine and Medical Research ◽

10.11603/ijmmr.2413-6077.2019.2.10900 ◽

2020 ◽

Vol 5 (2) ◽

pp. 105-112 ◽

Cited By ~ 1

Author(s):

N. Ya. Golovenko ◽

T. A. Kabanova ◽

S. A. Andronati ◽

O. I. Halimova ◽

V. B. Larionov ◽

...

Keyword(s):

Acute Inflammation ◽

Experimental Models ◽

Paw Edema ◽

Preclinical Trial ◽

Rat Paw Edema ◽

Anti Inflammatory ◽

Rat Paw ◽

First Time ◽

Inflammatory Action

Background. Propoxazepam, 7-bromo-5-(2-chlorophenyl)-3-propoxy-1H-benzo[e][1,4]diazepin-2(3H)-one, is a promising analgesic and anticonvulsant and is on preclinical trial. Objective. The aim of the research was to study the anti-inflammatory and analgesic action of Propoxazepam. Methods. The anti-inflammatory action was evaluated by carrageenan induced rat paw edema, formalin-induced paw licking response in mice and bradykinin-induced pain response in rat models. Results. It was established for the first time that the administration of Propoxazepam caused a significant anti-inflammatory activity when tested in different in vivo chemical experimental models of induced inflammation, i.e. carrageenan-, bradykinin- and formalin-induced inflammation tests. Conclusions. Propoxazepam significantly reduced acute and sub-acute inflammation and proved its efficacy and similar to anti-inflammatory action.

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Anti-inflammatory effects of muramyl dipeptide in experimental models of acute inflammation

Agents and Actions ◽

10.1007/bf01966836 ◽

1984 ◽

Vol 14 (1) ◽

pp. 72-75 ◽

Cited By ~ 4

Author(s):

Z. Zídek ◽

K. Mašek ◽

F. Šedivý

Keyword(s):

Acute Inflammation ◽

Muramyl Dipeptide ◽

Experimental Models ◽

Anti Inflammatory

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Jobelyn® exhibited anti-inflammatory, antioxidant, and membrane-stabilizing activities in experimental models

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2014-0113 ◽

2015 ◽

Vol 26 (5) ◽

Cited By ~ 5

Author(s):

Solomon Umukoro ◽

Oluwafemi Gabriel Oluwole ◽

Anthony T. Eduviere ◽

Omogbiya Itievere Adrian ◽

Abayomi M. Ajayi

Keyword(s):

Chronic Inflammation ◽

Acute Inflammation ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Experimental Models ◽

Hypotonic Medium ◽

Air Pouch ◽

Anti Inflammatory ◽

Air Pouch Model

AbstractJobelynAcute inflammation was induced with intraplanter injection of carrageenan and increase in rat paw volume was measured using plethysmometer. The volume of fluid exudates, number of leukocytes, concentrations of malondialdehyde (MDA), and glutathione (GSH) in the fluid were measured on day 5 after induction of chronic inflammation with carrageenan in the granuloma air pouch model. RBC lysis induced by hypotonic medium as determined by release of hemoglobin was measured spectrophotometerically.JB (50–200 mg/kg) given orally produced a significant inhibition of acute inflammation induced by carrageenan in rats. It reduced the volume and number of leukocytes in inflammatory fluid in the granuloma air pouch model of chronic inflammation. It further decreased the levels of MDA in the fluid suggesting antioxidant property. JB elevated the concentrations of GSH in inflammatory exudates indicating free radical scavenging activity. It also significantly inhibited RBC lysis caused by hypotonic medium, suggesting membrane-stabilizing property.JB has in vivo anti-inflammatory activity, which may be related to its antioxidant and membrane-stabilizing properties, supporting its use for the treatment of arthritic disorder.

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ACE2 as therapeutic agent

Clinical Science ◽

10.1042/cs20200570 ◽

2020 ◽

Vol 134 (19) ◽

pp. 2581-2595

Author(s):

Qiuhong Li ◽

Maria B. Grant ◽

Elaine M. Richards ◽

Mohan K. Raizada

Keyword(s):

Therapeutic Agent ◽

Renin Angiotensin System ◽

Peptide Hormone ◽

Experimental Models ◽

Electrolyte Balance ◽

Ang Ii ◽

Angiotensin Converting Enzyme 2 ◽

Beneficial Effects ◽

Overwhelming Evidence ◽

Future Challenges

Abstract The angiotensin-converting enzyme 2 (ACE2) has emerged as a critical regulator of the renin–angiotensin system (RAS), which plays important roles in cardiovascular homeostasis by regulating vascular tone, fluid and electrolyte balance. ACE2 functions as a carboxymonopeptidase hydrolyzing the cleavage of a single C-terminal residue from Angiotensin-II (Ang-II), the key peptide hormone of RAS, to form Angiotensin-(1-7) (Ang-(1-7)), which binds to the G-protein–coupled Mas receptor and activates signaling pathways that counteract the pathways activated by Ang-II. ACE2 is expressed in a variety of tissues and overwhelming evidence substantiates the beneficial effects of enhancing ACE2/Ang-(1-7)/Mas axis under many pathological conditions in these tissues in experimental models. This review will provide a succinct overview on current strategies to enhance ACE2 as therapeutic agent, and discuss limitations and future challenges. ACE2 also has other functions, such as acting as a co-factor for amino acid transport and being exploited by the severe acute respiratory syndrome coronaviruses (SARS-CoVs) as cellular entry receptor, the implications of these functions in development of ACE2-based therapeutics will also be discussed.

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