Lipoprotein(a), its autoantibodies, and circulating T lymphocyte subpopulations as independent risk factors for coronary artery atherosclerosis

2016 ◽  
Vol 88 (9) ◽  
pp. 31-38 ◽  
Author(s):  
O I Afanasievа ◽  
E A Pylaeva ◽  
E A Klesareva ◽  
A V Potekhina ◽  
S I Provatorov ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Lymphocyte Activation ◽  
Blood Lipid ◽  
Lymphocyte Subpopulations ◽  
Blood Analysis ◽  
Lipoprotein A ◽  
T Lymphocyte Subpopulations ◽  
Coronary Artery Atherosclerosis ◽  
Independent Risk Factors

Aim. To study the role of lipoprotein(a) [Lp(a)] as a potential autoantigen causing the activation of immunocompetent cells in atherosclerosis. Subjects and methods. A total of 104 men with stable coronary artery (CA) disease and different degrees of progressive coronary atherosclerosis were examined. Clinical blood analysis was carried out and lymphocyte subpopulations (CD4+, Th1, Th17, and Treg) were determined using immunofluorescence and flow cytometry. In addition, the indicators of blood lipid composition, Lp(a), autoantibody (autoAb) titer to Lp(a), and low-density lipoproteins (LDL), and the lymphocyte activation marker sCD25 were also measured. Results. The Lp(a) level was shown to predict the severity of CA lesions (β=0.28, p

Coronary Artery Atherosclerosis in Hypertensive Patients: The Role of Fibrinogen Genetic Variability

2017 ◽  
Vol 70 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Nikolaos Papageorgiou ◽  
Alexandros Briasoulis ◽  
Georgios Hatzis ◽  
Emmanuel Androulakis ◽  
Maria Kozanitou ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Genetic Variability ◽  
Hypertensive Patients ◽  
Coronary Artery Atherosclerosis

Role of measurement of fractional flow reserve in coronary artery atherosclerosis

2015 ◽  
Vol 87 (9) ◽  
pp. 106 ◽  
Author(s):  
F. Yu. Kopylov ◽  
A. A. Bykova ◽  
Yu. V. Vasilevsky ◽  
S. S. Simakov
Keyword(s):  
Coronary Artery ◽  
Fractional Flow Reserve ◽  
Fractional Flow ◽  
Flow Reserve ◽  
Coronary Artery Atherosclerosis

Impaired Immunocompetence in Patients with Malignant Gliomas

Neurosurgery ◽  
1982 ◽  
Vol 10 (5) ◽  
pp. 571-573 ◽  
Author(s):  
Massimo A. Gerosa ◽  
Alessandro Olivi ◽  
Mark L. Rosenblum ◽  
Gian Piero Semenzato ◽  
Antonio Pezzutto
Keyword(s):  
T Lymphocyte ◽  
Peripheral Blood ◽  
Radiation Treatment ◽  
Malignant Gliomas ◽  
Suppressor Cells ◽  
Lymphocyte Subpopulations ◽  
Helper Cells ◽  
T Lymphocyte Subpopulations ◽  
Suppressor Lymphocytes

Abstract T-lymphocyte subpopulations (Tg or “suppressor cells” and Tm or “helper cells”) in the peripheral blood of 16 patients with malignant intracranial gliomas were analyzed. The number of Tm-lymphocytes was very close to normal in both preand postoperative samples, whereas Tg-lymphocytes were significantly increased preoperatively (18.55 ± 1.11 vs. 12.72 ± 1.19, p < 0.001) and were still slightly higher than normal after operation and radiation treatment. These preliminary findings suggest a possible role of T-suppressor lymphocytes in the impairment of cell-mediated immunocompetence that has been observed repeatedly in patients with malignant gliomas.

scholarly journals Circulating ANGPTL3 and ANGPTL4 levels predict coronary artery atherosclerosis severity

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Ting Sun ◽  
Wanlin Zhan ◽  
Lijiang Wei ◽  
Zuojun Xu ◽  
Li Fan ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Coronary Atherosclerosis ◽  
Coronary Artery Stenosis ◽  
Plasma Concentrations ◽  
Artery Stenosis ◽  
Coronary Artery Atherosclerosis ◽  
Vascular Stenosis ◽  
Independent Risk Factors ◽  
Atherosclerosis Development ◽  
Diagnostic Coronary Angiography

Abstract Background We investigated the role of ANGPTL3 and ANGPTL4 in atherosclerosis development and determined whether plasma concentrations of ANGPTL3 and ANGPTL4 are related to the degree of coronary stenosis. Methods A total of 305 consecutive patients with angina who underwent diagnostic coronary angiography were enrolled in the study between August 2017 and August 2018. The levels of ANGPTL3 and ANGPTL4 were measured by using competitive ELISA kits. Results According to the degree of coronary artery stenosis, patients were classified into four types: coronary artery stenosis of < 10%, 10-50%, 50-75, and > 75%. The plasma ANGPTL3 level was higher (51.71 ± 52.67 vs. 24.65 ± 10.32 ng/mL, P < 0.001) and that of ANGPTL4 was lower (454.66 ± 269.05 vs. 875.49 ± 961.15 ng/mL, P < 0.001) in the coronary artery stenosis ≥ 10% group than in the < 10% group. ANGPTL3 and ANGPTL4 levels were significantly associated with the severity of coronary vascular stenosis. ROC curve analyses indicated that ANGPTL3 concentrations above 30.5 ng/mL can predict atherosclerosis with a sensitivity of 71.2% and specificity of 75.3%, and that ANGPTL4 levels below 497.5 ng/mL can predict atherosclerosis with a sensitivity of 63.9% and specificity of 74.5%. ANGPTL3 and ANGPTL4 were determined to be independent risk factors for coronary atherosclerosis with odds ratios (ORs) of 0.189 (95% CI 0.097-0.368, P < 0.001) and 3.625 (95% CI 1.873-7.016, P < 0.001), respectively. Conclusions Increased ANGPTL3 or decreased ANGPTL4 shows an association with coronary atherosclerosis and, may become a predictor of coronary atherosclerosis in the future.

Impaired T-Lymphocyte Subpopulations and Deranged Profile of Immunologic Activation in Patients with Gaucher Disease Type I

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4915-4915
Author(s):  
Argiris S Symeonidis ◽  
George Theodorou ◽  
Constantina Repa ◽  
Theodore Marinakis ◽  
Panayiotis Tsaftaridis ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Gaucher Disease ◽  
Lymphocyte Activation ◽  
Absolute Number ◽  
Lymphocyte Subpopulations ◽  
Type I ◽  
Cd8 T Lymphocytes ◽  
T Lymphocyte Subpopulations ◽  
Hla Dr

Abstract Patients with Gaucher disease exhibit substantial evidence of impairment of their immune system, namely, increased serum levels of proinflammatory cytokines and immunoglobulins, and increased incidence of B-cell malignancies, such as non-Hodgkin’s lymphoma, MGUS and multiple myeloma. We investigated peripheral blood T-lymphocyte subpopulations with dual color flow cytometry, as well as the direction of T-lymphocyte activation, by using intracytoplasmic immunostaining for IL-2, IL-4, IL-10 and IFN-gamma, on resting CD4+ and CD8+ T-lymphocytes and following activation with PMA- 1 with the presence of Brefeldin-A. Evaluations were performed on 16 patients with type I Gaucher disease and on 17 healthy controls. Patients had significantly decreased absolute lymphocyte count (1621±684 vs 2148±566/mm3, p=0.013), CD3+ (1197±478 vs 1508±431/mm3, p=0.045) and CD4+ T-lymphocytes (658±245 vs 945±253/mm3, p=0.021), but not CD8+ T-lymphocytes (491±331 vs 486±189/mm3, p: n.s.), resulting in a significant reduction of the CD4/CD8 ratio (1.59±0.68 vs 2.16±0.83, p=0.041). The populations of naive CD4+CD45RA+ and of memory CD4+CD45RO+ T-lymphocytes were also significantly decreased (218±128 vs 432±179/mm3, p=0.0005 and 484±185 vs 631±231/mm3, p=0.056 respectively), however, CD8+CD45RA+ and CD8+CD45RO+ subpopulations did nor differ significantly, when compared to controls. CD3−CD56+, but not CD3+CD56+ lymphocytes were also decreased (131±82 vs 199±97/mm3, p=0.037). Patients had higher percentages of CD8+ (29.2±9.7 vs 23.5±6.8%, p=0.042), CD8+CD45RA+ (22.1±6.2 vs 18.3±5.0%, p=0.046) and CD8+CD45RO+ T-lymphocytes (13.2±6.2 vs 9.6±3.7%, p=0.027), as well as of activated CD8+HLA-DR+ (0.93±0.68 vs 0.48±0.21%, p=0.008) and CD4+HLA-DR+ T-lymphocytes (1.77±0.93 vs 1.09±0.48%, p=0.008). Moreover, although both, the absolute number and the percentage of CD20+ B-lymphocytes were similar, patients exhibited significantly increased absolute number and percentage of CD5+CD20+ B-lymphocytes (1.63±0.55 vs 0.64±0.37% p=0.00002 and 29±20 vs 13±8/mm3, p=0.011, respectively). Finally, patients with Gaucher disease had significantly increased resting TH2-polarized CD4+T-lymphocytes (CD4+IL-10+: 0.41±0.29 vs 0.24±0.11%, p=0.045) and TH1-polarized CD8+ T-lymphocytes (CD8+IFNγ+: 0.15±0.07 vs 0.08±0.04%, p=0.005, CD8+IL10+: 0.22±0.08 vs 0.32±0.014, p=0.052, and IFNγ+/IL4+ ratio among the CD8+ population 2.54±2.1 vs 1.08±0.91, p=0.018). Following mitogenic activation a very significant impairment of obtaining the TH1 phenotype was observed (CD4+IL2+ lymphocytes 33.7±17.1 vs 65.4±6.1%, p&lt;0.00001). The above findings suggest that in patients with Gaucher disease there is a significant numerical impairment of T-helper lymphocytes and a shift towards TH-2 direction of lymphocyte activation. These findings may explain the rarity of autoimmune manifestations despite the chronic inflammatory reaction, as well as the increased incidence of lymphoid malignancies, which has been reported among patients suffering from this disease.

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