scholarly journals Influence of tannin addition on the content and composition of polyphenolic compounds in wines

2009 ◽  
Vol 26 (Special Issue) ◽  
pp. S33-S38 ◽  
Author(s):  
M. Cíchová ◽  
J. Petříček ◽  
J. Fiala
Keyword(s):  
Solid Phase ◽  
Polyphenolic Compounds ◽  
Diode Array ◽  
Diode Array Detection ◽  
Rp Hplc ◽  
Array Detection ◽  
Flavour Profile ◽  
The Individual ◽  
Rosé Wine ◽  
Complicated Matrices

Polyphenols can greatly affect the sensorial characteristics and stability of wine. The concentration of polyphenols in wines is very low, the sample must be thus concentrated before the analytical measurement. The extraction on solid phase (SPE) is a suitable method for the isolation, purification, and concentration of polyphenols from complicated matrices. RP-HPLC with diode array detection was used for the separation and identification of polyphenols. A library of absorption spectra of standards was created and used for the identification of 14 polyphenols in wines. The contents of the individual polyphenols and their changes after the addition of four tannin preparations were determined in eight white and two rosé wine samples. The influence of the flavour profile of the applied tannin preparations on sensorial characteristics of wines was established

scholarly journals Tetrahydropalmatine Poisoning: Diagnoses of Nine Adult Overdoses Based on Toxicology Screens by HPLC with Diode-Array Detection and Gas Chromatography–Mass Spectrometry

1999 ◽  
Vol 45 (2) ◽  
pp. 229-236 ◽  
Author(s):  
Chi-Kong Lai ◽  
Albert Yan-Wo Chan
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Diode Array ◽  
Diode Array Detection ◽  
Chromatography Mass Spectrometry ◽  
Polar Metabolites ◽  
Array Detection ◽  
Neurological Disturbance

Abstract Background: Tetrahydropalmatine (THP) is a neuroactive alkaloid with analgesic and hypnotic action. Its analysis is important because cases of human poisonings have emerged as a result of unregulated use of some proprietary biopharmaceuticals containing purified THP. Methods: We established analytical parameters for HPLC with diode-array detection (HPLC-DAD) and gas chromatography–mass spectrometry (GC-MS) for the detection of THP in serum and urine. Nine acutely THP-poisoned adults were thus screened over 16 months. Results: All patients recovered quickly after mild neurological disturbance. In general, THP was metabolized rapidly and excreted as polar metabolites in urine. Serum THP was measured in five cases and found to be <0.1–1.2 mg/L (<0.3–3.4 μmol/L). Paired analyses of urine with and without glucuronidase treatment clarified the disposition of THP. Our GC–MS method with trimethylsilane derivatization identified O-desmethyl metabolites. With a uniform solid-phase extraction, the HPLC-DAD procedure detected intact glucuronide metabolites. Conclusion: Intact glucuronide metabolites of THP are sensitive markers for THP exposures. Our methods and findings provide practical tools and information for surveillance of intoxication caused by excessive THP intake.

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