scholarly journals Differences in phenolic content and antioxidant activity in yellow and purple-fleshed potatoes grown in the Czech Republic

2008 ◽  
Vol 54 (No. 1) ◽  
pp. 1-6 ◽  
Author(s):  
J. Lachman ◽  
K. Hamouz ◽  
M. Šulc ◽  
M. Orsák ◽  
P. Dvořák
Keyword(s):  
Antioxidant Activity ◽  
Czech Republic ◽  
Phenolic Content ◽  
Field Trials ◽  
Total Phenolic ◽  
Dpph Assay ◽  
The Czech Republic ◽  
Freeze Dried ◽  
Significant Difference ◽  
Lower Variation

The aim of this study was to determine the total phenolic (TP) content and antioxidant activity (AA) in yellow (cvs. Impala, Karin, Dita, Saturna) and purple-fleshed (cvs. Valfi, Violette) potatoes grown in the Czech Republic in 2004 in four locations in precise field trials. TP content was determined by Folin-Ciocalteau assay and AA by DPPH assay both in freeze-dried tuber matter. Results showed a statistical significant difference in TP content and AA between yellow and purple-fleshed potatoes. Purple-fleshed cultivars showed higher TP content (by 60%) than yellow-fleshed cultivars; AA in purple-fleshed cultivars was twice as high as in yellow-fleshed potatoes. A medium linear correlation between TP and AA was found ( <I>r</I><sup>2</sup> = 0.747). Average TP content in yellow-fleshed cultivars was 2.96 GAE (g of gallic acid per kg dm); in purple-fleshed cultivars it was 4.68 GAE. Average AA in yellow-fleshed cultivars was 11.26 EAA (mg of ascorbic acid per 100 g dm) and in purple-fleshed cv. 24.79 EAA. Purple-fleshed potatoes showed a lower variation among localities (only 6%). Hence, regarding a relatively high potato intake by Czechs (72 kg per capita a year), contribution of potatoes, especially purple-fleshed, to total antioxidants intake should be considered.

scholarly journals Effect of Freeze, Oven and Microwave Pretreated Oven Drying on Color, Browning Index, Phenolic Compounds and Antioxidant Activity of Hawthorn (Crataegus orientalis) Fruit

2018 ◽  
Vol 46 (2) ◽  
pp. 449-456 ◽  
Author(s):  
Hacer COKLAR ◽  
Mehmet AKBULUT ◽  
Semih KILINC ◽  
Ali YILDIRIM ◽  
Iliasu ALHASSAN
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Drying Methods ◽  
Drying Method ◽  
Microwave Pretreatment ◽  
Oven Drying ◽  
Freeze Dried

Flowers, leaves and fruits of hawthorn plant are traditionally used for treating diseases like hypertension and atherosclerosis. The medicinal effects of the plant are generally attributed to its phenolic compounds. However, the fruits are perishable materials because of their high content of water, and generally dried and stored to be used outside its season. The main aim of this research was to investigate the effect of different drying methods on phenolic compounds of the hawthorn fruit. Fruits were collected from the wild growing trees in Turkey. De-seeded fruits were dried in freeze-, oven- (60 oC) and microwave pretreated oven drying (microwave application for 5 min at 360 W before drying at 60 oC) methods and analyzed for antioxidant activity, phenolic compounds, total phenolic content and color parameters. Total phenolic content of fresh hawthorn fruits was found as 13.36 mg g-1 DW. Oven- and microwave pretreated oven drying methods had a reductive effect on total phenolic content and antioxidant activity of fruits when compared to freeze drying method. (-)-Epicatechin (994.10 mg kg-1 DW), rutin (765.30 mg kg-1 DW), and procyanidin B2 (553.80 mg kg-1 DW) were the main phenolics of the fruit. Lowest values of these three compounds were observed in oven-dried fruits. Microwave pretreatment oven drying method resulted in browner product. Although the highest phenolic concentration and antioxidant activity were occurred in freeze-dried sample, microwave pretreatment before oven drying could be applied to reduce the time and cost of drying in terms of phenolic compounds and antioxidant activity.

scholarly journals Changes of antioxidant activity in honey after heat treatment

2013 ◽  
Vol 31 (No. 6) ◽  
pp. 601-606 ◽  
Author(s):  
G. Šarić ◽  
K. Marković ◽  
D. Vukičević ◽  
E. Lež ◽  
M. Hruškar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Significant Difference ◽  
Acacia Honey ◽  
Different Temperatures ◽  
Ferric Reducing Antioxidant Power

We determined how the antioxidant activity and total phenolic content of honey changed after being subjected to a high temperature. Antioxidant activity was determined using two methods &ndash; FRAP (ferric reducing antioxidant power) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. Total phenolic content was determined by modified Folin-Ciocalteu method. The research was conducted on 31 samples of acacia honey and 8 samples of chestnut honey. All measurements were done at two temperatures &ndash; at 23&deg;C (room temperature) and after 5 min of heating at 95&deg;C. The obtained results show uneven changes of antioxidant activity and total phenolic content among individual samples, i.e. in some samples antioxidant activity decreased after heating, while in others it increased. The same applies to the total phenolic content. Statistical analysis of the results (t-test) showed no statistically significant differences between the results measured at two different temperatures (P &gt; 0.05) in all three methods used, and in both types of honey. The only statistically significant difference (P &lt; 0.05) was observed when using DPPH method in acacia honey.

Antioxidant activity, total phenolic and flavonoids contents of three herbs used as condiments and additives in pickles products

2013 ◽  
Vol 59 (3) ◽  
pp. 51-62 ◽  
Author(s):  
Abdollah Ghasemi Pirbalouti ◽  
Milad Setayesh ◽  
Amir Siahpoosh ◽  
Hamid Mashayekhi
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Food Industry ◽  
Phenolic Content ◽  
Food Additives ◽  
Total Phenolic ◽  
Endemic Plants ◽  
Dpph Assay ◽  
Frap Assay

Abstract Heracleum lasiopetalum Boiss, Kelussia odoratissima Mozaff., and Echinophora platyloba DC. belong the Apiaceae family. They are Iranian endemic plants. These three herbs have been used as food additives in traditional preparations such as pickles. Antioxidant activity (AA) of methanol extracts (ME) of the plants was evaluated by three assays, including DPPH, FRAP, and TEAC. From all three assays, comparing all the MEs for their IC50 and EC1 values, E. platyloba had the highest AA. Total phenolic content (TPC) of the extracts ranged from 74 to 120 mg TAE/g. The extract of H. lasiopetalum exhibited the highest TPC. The flavonoids content (FC) of the extracts ranged from 7.63 to 14.52 mg RE/g, from which the extract of E. platyloba had the highest flavonoids concentration. A positive correlation between the FC and AA in DPPH assay was found. A significant correlation was also found between the TPC and AA in FRAP assay. These results suggested that the level of AA in these plants varied in a great extent. Our results indicated that extract of E. platyloba could be an important dietary source of flavonoids compounds with high antioxidant capacity. In addition, E. platyloba can be used as an alternative preservative and natural flavor instead of synthetic ones in food industry (especially pickles)

Chromatographic Assessment of Polyphenolic Profile and Total Phenolic Content and Antioxidant Activity of Common Leafy Vegetables in Bangladesh

2020 ◽  
Vol 7 (1) ◽  
pp. 40-50
Author(s):  
Mohammad Khairul Alam ◽  
Rumana Tuli ◽  
Mohammad Sharif Khan ◽  
Abu Tareq Mohammad Abdullah ◽  
Maksuda Khatun ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chronic Diseases ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Centella Asiatica ◽  
Leafy Vegetables ◽  
Total Phenolic ◽  
Polyphenolic Composition ◽  
Freeze Dried

Background: Polyphenolic compounds are known to provide health benefits and protect against degenerative chronic diseases. Utilization and identification of foods with a high content of these compounds are gaining greater attention nowadays. Objective: The present study reports the total phenolic content (TPC), polyphenolic composition and antioxidant activity (DPPH, FRAP and TEAC) of 10 commonly consumed leafy vegetables growing in Bangladesh. Materials and Methods: The samples were collected from different locations of Bangladesh and mixed together to ensure sample representativeness. Folin-Ciocalteu method was used for the analysis of TPC, and quantification of polyphenolic components was done by high-performance liquid chromatography (HPLC- DAD). Additionally, antioxidant activities of the selected vegetables were also analysed by utilizing DPPH, FRAP & TEAC. Results and Discussion: TPC ranged from 23.64 ± 1.20 to 45.59 ± 3.04 mg gallic acid equivalent (GAE)/g freeze-dried sample (fds). The polyphenolic spectrum ranged from 0.30 ± 0.02 to 647.42 ± 147.12 mg/100 g fds; quantity and spectrum of which varied in the vegetables. Among the studied vegetables, Centella asiatica contained the highest amount of TPC (45.59 ± 3.04 mg GAE/g fds) and also exhibited high antioxidant capacities, as documented by DPPH, FRAP and TEAC assays. Moreover, Principal component analysis (PCA) of investigated variables clearly separated Centella asiatica from other samples. Conclusion: Phenolic compounds being strong antioxidants reduce the risk of chronic diseases and the finding of this study would aware the people to take vegetables rich in phenolics. It would also fill up the data gap in the existing food composition table of Bangladesh.

scholarly journals The antioxidant properties and α-amylase inhibition activities of polyphyto mixture with honey formulations

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2190-2196
Author(s):  
W.N.A.S.W.M. Rashidi ◽  
N. Muhammad ◽  
N. Abdullah ◽  
B.A. Talip ◽  
N. Bahrin
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibition ◽  
Interaction Effect ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Inhibition Activity ◽  
Dpph Assay ◽  
Ficus Deltoidea

Ficus carica, Orthosiphon stamineus, Ficus deltoidea and Trigona honey are known for their high antioxidant amount. In this research, these plants and honey were used to study their interactions in terms of the antioxidant properties and the inhibition of α-amylase enzyme. These plants samples were chosen as they are usually used in traditional medicine to treat diabetes. Therefore, the objectives of this study are to measure the antioxidant activity as well as the α-amylase enzyme inhibition properties of the polyphyto formulations and Trigona honey. Simplex Centroid Mixture Design (SCMD) was used to design the formulations. In this experiment, the highest (p<0.05) antioxidant content for 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was a formulation of 100% Trigona honey (90.09%). Meanwhile, a mixture of 50% F. carica – 50% O. stamineus and 50% O. stamineus – 50% Trigona honey yield the highest (p<0.05) total phenolic content (TPC) value (133.77 and 133.17 μg/mL, respectively), which indicated a synergistic interaction effect. However, for the measurement of ferric reduction antioxidant potential (FRAP), the formulation of 100% O. stamineus and 100% F. deltoidea exhibits the highest (p<0.05) value (148.95 and 148.78 μg/mL, respectively). In terms of enzyme inhibition, F. deltoidea showed the highest inhibition activity for α-amylase (8.826%). In conclusion, different percentages of F. carica, O. stamineus, F. deltoidea and Trigona honey have different interaction effects on the antioxidant activity and α-amylase inhibition depending on the amount of each factor.

scholarly journals Optimization of the extraction of phenolic compounds and antioxidant activity from the roots of Waltheria ovata using the response surface methodology

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2095-2102
Author(s):  
O. Herrera-Calderon ◽  
R. Vega
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Sample Ratio

Waltheria ovata is a medicinal plant belonging to the Sterculiaceae genus. Natural products of Waltheria ovata could be used in the food industry as natural antioxidants due to its high content of polyphenols according to the literature. The main objective in this research was to optimize the extraction of phenolic compounds and the antioxidant activity from Waltheria ovata roots using response surface methodology (RSM). The total phenolic content in different extracts was determined by spectrophotometric method (Folin-Ciocalteu reagent) and the antioxidant activity by using DPPH assay. To optimize the conditions for total phenolic content and antioxidant activity were used three independent variables: solvent/sample ratio (1:10, 1:20 and 1:30 g/mL), temperature (40, 50, and 60°C) and time (40, 50 and 60 mins). The results showed that total phenolic content and antioxidant activity in the experiments ranged from 8.7 to 12.1 mg GAE/g and 76.1% to 96.7%, respectively. The coefficients of determination (R2 values) for phenolic content and antioxidant activity were 0.86 and 0.91, respectively. Under the optimum conditions of 1:20 g/mL, 60°C and 55 mins of extraction, the values for total phenolic content and antioxidant activity were 0.448±0.02 mg GAE/g and 87.00±2.0%, respectively. These data showed that the experimental responses were reasonably close to the predicted responses (0.444 mg GAE/g and 84.67%). Therefore, the results showed that Waltheria ovata can be used as antioxidant in foods.

scholarly journals Bekul Fruit, Potential Pharmafood from Northern Region of Bali Island, Indonesia: Selected Phytochemical Analyses and Antioxidant Activity of Its Ethanol Extract.

2021 ◽  
Vol 14 (1) ◽  
pp. 477-483
Author(s):  
Agung Nova Mahendra ◽  
I Nyoman Tri Pramartha
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Activity Index ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Northern Region ◽  
Total Phenolic ◽  
Tannin Content ◽  
Dpph Assay ◽  
Gallic Acid Equivalent

Background: Bekulfruit from Northern region (Buleleng regency), Bali, Indonesia, is commonly consumed fresh in the island of Bali or processed as local delicacy and used as part of religious offerings. Up to date, there is no data regarding the taxonomy, phytochemical composition, and antioxidant properties of this Balinese fruit.This study was aimed to investigate total phenolic content, tannin content and antioxidant activity of the ethanol extract of bekul fruit obtained from Banjar district, Buleleng regency, Bali. Methods: Total phenolic compound was quantified in terms of gallic acid equivalent (GAE) by using Folin-Ciocalteu method, mean while tannin content was determined in terms of tannic acid equivalent (TAE). IC50of the extract was determined using DPPH assay, and subsequently used in the calculation of antioxidant activity index (AAI) using the formula of Scherer and Godoy (2009). Results: Bekul plant was revealed as Ziziphusjujuba Mill. Total phenolic and tannin content of the extract was 29.48 mg/100 g GAE and 91.06 mg/100 g TAE, respectively. Thevalue of IC50was 77.40 mg/ml, with antioxidant activity index (AAI) of 50.94. Conclusion:Ethanol extract of bekul (Ziziphusjujuba Mill.) fruit contains phenolic and tannin compounds. This extract is found to scavenge free radicals and possess very strong antioxidant activityin vitro. Taken together, these findings lead to the notion that bekul fruit from Northern region of Bali, Indonesia, is a promising pharma food

Total Phenolic Content and Free Radical Scavenging Activity of Nelumbo nucifera Gaertn.

2019 ◽  
Vol 886 ◽  
pp. 52-55
Author(s):  
Khemjira Jarmkom ◽  
Nakuntwalai Wisidsri ◽  
Pattaranut Eakwaropas ◽  
Warachate Khobjai
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Significant Difference

All parts of N. nucifera are used as oriental medicine for various medicinal purposes. The aimed of this study was to determine phenolic compound and antioxidant activity of different parts of three lotus (N. nucifera including of Roseum Plenum (RP), Album Plenum (AP), and Hindu Lotus (HL)). Total phenolic content using Folin-Ciocalteu reagent method and free radical scavenging activities using DPPH assay were determined. Three deferent parts of lotus (leaves, stem, and flower) were determined. The result indicated that Roseum Plenum leaf showed the highest total phenolic contents and Roseum Plenum flower showed the highest antioxidant activities. Total phenolic content of different part of different of N. nucifera showed a significant difference (p < 0.05). It also was not uniform when compare between different of N. Nucifera. The results found that the total phenolic content was the opposite of antioxidant activity. This result may be attributed to antioxidant activity may not be from phenolic content. Therefore, it is important to research and develop the potential of lotus extraction in the future.

scholarly journals Effect of Location on Physico-Chemical, Cooking and Antioxidant Properties of Variously-Treated and Milled Indian Rice Cultivars

2018 ◽  
Vol 6 (1) ◽  
pp. 183-190
Author(s):  
Rahul Thory ◽  
Kawaljit Singh Sandhu ◽  
Archana Sinhmar
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Rice Cultivar ◽  
Total Phenolic ◽  
Oil Absorption ◽  
Scavenging Activity ◽  
Rice Cultivars ◽  
Physico Chemical ◽  
Significant Difference

Rice cultivar, cv.PR-118 was grown in two different locations [abbreviated as cv.PR-118 (Pb.) and cv.PR-118 (Hr.)] and three different milling treatments were given. In the first treatment, the cultivars was normally milled, second treatment involved, parboiling and then milling, third included germination and then milling. These were then studied for the effect of location on their physico-chemical, functional, cooking and antioxidant properties. Flour from cv.PR-118 (Pb.) showed highest bulk density, water and oil absorption capacities as compared to cv. PR-118 (Hr.) for normal, parboiled and germinated samples. Grains from cv.PR-118 (Hr.) took more time to cook as compared to cv.PR-118 (Pb.) for all the treatments. Significant difference (p 0.05) was observed in antioxidant activity of cv.PR-118 grown in two locations. cv.PR-118 (Pb.) showed the highest value for ABTS+ scavenging activity as compared to cv.PR-118 (Hr.). In NPR, NBR, PPR and PBR fractions, cv.PR-118 (Hr.) showed higher values (702.3 and 588.2 µg GAE/g, respectively) for total phenolic content. Among GPR fraction, cv.PR-118 (Pb.) showed higher TPC value as compared to cv.PR-118 (Hr.).

scholarly journals Basil – a comparison of the total phenolic content and antioxidant activity in selected cultivars

10.5219/1623 ◽  
2021 ◽  
Vol 15 ◽  
pp. 445-452
Author(s):  
Martin Adámek ◽  
Jiří Mlček ◽  
Anna Adámková ◽  
Vít Guiglielmo Mišurec ◽  
Jana Orsavová ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Human Body ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Content ◽  
Total Phenolic ◽  
Cold Temperatures ◽  
Significant Difference ◽  
Phenolic Substances

During the lifetime, the human body forms a considerable amount of free radicals damaging DNA, cell membranes, and their components. A wider application of basil (Ocimum basilicum), an aromatic plant and one of the common gastronomic commodities in the human diet could help to prevent the formation of free radicals and to remove them from the human body. Therefore, determination of antioxidant activity and total content of phenolic substances in selected cultivars of basil (Ohře, Sweet green, Salad leaf, Purple opal, Thai) in a fresh and frozen state and the mixture with another plant (garlic, mint, rocket, spinach) in the fresh and refrigerated state was performed. The total content of phenolic substances in basil was established by the spectrophotometric Folin-Ciocalteu method. DPPH spectrophotometric method was used to analyze the antioxidant activity. The results have shown statistically significant differences between basil cultivars under the same growing conditions. The values of antioxidant activity in frozen samples ranged from 5.1 ±0.4 mg.g-1 AA FW to 11.71 ±0.18 mg.g-1 AA FW and the total phenolic content varied between 2.77 ±0.16 mg.g-1 GAE FW in TH and 8.93 ±0.13 mg.g-1 GAE FW. A statistically significant difference between fresh and frozen samples was established only in the “Ohře” cultivar. After the storage in cold temperatures, all mixtures showed a reduction in the antioxidant activity and total content of phenolic substances. The mixture of basil and mint performed the highest values of antioxidant activity and total content of phenolic substances and significantly differed from the other mixtures most often. The addition of basil and its mixtures to food and beverages can substantially increase their biological value and subsequently also the quality of human nutrition.

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