Association between the HMW-glutenin subunits and gluten strength characteristics in khorassan wheat lines - Short Communications

Khorassan wheat (Triticum turgidum ssp. turanicum Jakubz em. A. Löve & D. Löve) is an ancient tetraploid wheat that was grown in the Mediterranean region and Near East. Sixteen lines differing in the composition of high-molecular-weight glutenin subunits (HMWGs) were evaluated for SDS-sedimentation volume and quality index (QI). The data suggested that the two subunit combinations detected in the examined materials at the Glu-B1 locus showed differences in both characteristics (relatively higher levels at the presence of the subunit combination 7+15 compared to 6+8). Weak gluten is in general characteristic of this wheat species. It could be used in a better way for other baking applications than for the pasta industry.

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Variation of high molecular weight glutenin subunits in two neglected tetraploid wheat subspecies

Czech Journal of Genetics and Plant Breeding ◽

10.17221/61/2008-cjgpb ◽

2009 ◽

Vol 44 (No. 4) ◽

pp. 140-146 ◽

Cited By ~ 4

Author(s):

L. Caballero ◽

L.M. Martín ◽

J.B. Alvarez

Keyword(s):

Genetic Diversity ◽

Triticum Turgidum ◽

Tetraploid Wheat ◽

Glutenin Subunits ◽

Bread Making ◽

Allelic Variants ◽

Passport Data ◽

Hmw Glutenin ◽

Novel Alleles ◽

New Alleles

The genetic diversity of 140 accessions of Triticum turgidum ssp. carthlicum Nevski em. A. Löve & D. Löve and 159 accessions of T. turgidum ssp. polonicum L. em. Thell. was evaluated by the analysis of HMW glutenin subunits. Seven allelic variants were found among the carthlicum accessions: three at the Glu-A1 locus (two of them were novel alleles) and four at the Glu-B1 locus (one of them novel). More variability was found among the polonicum accessions with 16 allelic variants: six at the Glu-A1 locus (three of them novel), and ten at the Glu-B1 locus (five of them novel). Totally, ten new alleles were found, one of which appeared in both subspecies. Out of 19 different combinations of alleles detected in both subspecies, 14 were novel. Based on the available passport data, the carthlicum accessions could be separated by origin into 18 groups, and the polonicum accessions into 33 such groups. The genetic diversity was lower among the carthlicum (Ht = 0.174) than among the polonicum accessions (Ht = 0.562). In both subspecies, most diversity was present between groups differing in origin, whereas diversity within the groups was very low. The detected variability offers possibilities for the improvement of bread making quality in durum wheat through introduction of newly detected alleles and for the broadening of genetic diversity in this wheat species.

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The Independent Domestication of Timopheev’s Wheat: Insights from Haplotype Analysis of the Brittle rachis 1 (BTR1-A) Gene

Genes ◽

10.3390/genes12030338 ◽

2021 ◽

Vol 12 (3) ◽

pp. 338

Author(s):

Moran Nave ◽

Mihriban Taş ◽

John Raupp ◽

Vijay K. Tiwari ◽

Hakan Ozkan ◽

...

Keyword(s):

Promoter Region ◽

Haplotype Analysis ◽

Common Ancestor ◽

Triticum Turgidum ◽

Tetraploid Wheat ◽

Wheat Species ◽

Loss Of Function ◽

Brittle Rachis ◽

Gene Level ◽

Large Panel

Triticum turgidum and T. timopheevii are two tetraploid wheat species sharing T. urartu as a common ancestor, and domesticated accessions from both of these allopolyploids exhibit nonbrittle rachis (i.e., nonshattering spikes). We previously described the loss-of-function mutations in the Brittle Rachis 1 genes BTR1-A and BTR1-B in the A and B subgenomes, respectively, that are responsible for this most visible domestication trait in T. turgidum. Resequencing of a large panel of wild and domesticated T. turgidum accessions subsequently led to the identification of the two progenitor haplotypes of the btr1-A and btr1-B domesticated alleles. Here, we extended the haplotype analysis to other T. turgidum subspecies and to the BTR1 homologues in the related T. timopheevii species. Our results showed that all the domesticated wheat subspecies within T. turgidum share common BTR1-A and BTR1-B haplotypes, confirming their common origin. In T. timopheevii, however, we identified a novel loss-of-function btr1-A allele underlying a partially brittle spike phenotype. This novel recessive allele appeared fixed within the pool of domesticated Timopheev’s wheat but was also carried by one wild timopheevii accession exhibiting partial brittleness. The promoter region for BTR1-B could not be amplified in any T. timopheevii accessions with any T. turgidum primer combination, exemplifying the gene-level distance between the two species. Altogether, our results support the concept of independent domestication processes for the two polyploid, wheat-related species.

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Composition of and variation in high- and low-molecular weight glutenin subunits, and omega gliadins in Ethiopian tetraploid wheat germplasm

Plant Genetic Resources ◽

10.1079/pgr2006110 ◽

2006 ◽

Vol 4 (2) ◽

pp. 134-143 ◽

Cited By ~ 7

Author(s):

Faris Hailu ◽

Eva Johansson ◽

Arnulf Merker ◽

Getachew Belay ◽

Harjit-Singh ◽

...

Keyword(s):

Molecular Weight ◽

Tetraploid Wheat ◽

Glutenin Subunit ◽

Low Molecular Weight ◽

Glutenin Subunits ◽

Hmw Glutenin Subunits ◽

Lmw Glutenin Subunits ◽

Hmw Glutenin ◽

Omega Gliadins ◽

High Degree

A collection of 120 Ethiopian tetraploid wheat accessions was analysed for high-molecular weight (HMW) glutenin subunit, low-molecular weight (LMW) glutenin subunit and omega gliadin composition by SDS–PAGE. For the HMW glutenin subunits, a new allelic variant, 2****, was detected which has not been previously described at the Glu-A1 locus. A high proportion of Glu-A1x banding pattern was observed in durum wheat. For the Glu-B1 locus four different banding patterns were detected. Among those HMW glutenin subunits, 7+8 were the most common, while subunits 14+15 and 6+8 were found to be rare. A high degree of variation was evident for the LMW glutenin subunits and D-zone omega gliadins. The association of the composition of the gluten with quality has been discussed. This wide variation can be used in improving the quality of wheat and to widen its genetic base.

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Variation in HMW glutenin subunits of different species of wheat

Plant Soil and Environment ◽

10.17221/4199-pse ◽

2011 ◽

Vol 48 (No. 1) ◽

pp. 15-19

Author(s):

Z. Gálová ◽

MichalíkI ◽

H. Knoblochová ◽

E. Gregová

Keyword(s):

Triticum Turgidum ◽

Wheat Cultivars ◽

Glutenin Subunits ◽

Bread Making ◽

Wheat Varieties ◽

Hmw Glutenin Subunits ◽

Sds Page ◽

Hmw Glutenin ◽

Triticum Durum Desf

Method ISTA SDS-PAGE was used for separation, detection and evaluation of high molecular weight glutenin subunits (HMW) in the different wheat species. The relation has been studied between the HMW glutenin subunit alleles and the bread-making quality of 25 world wheat cultivars and 21 regional varieties common wheat varieties (Triticum aestivum L.), 17 winter spelt wheat (Triticum spelta L.), 3 durum wheat cultivars (Triticum durum DESF.), 9 cultivars of Triticum turgidum L. and 5 cultivars of Triticum polonicum L. The highest frequency of occurrence of HMW glutenin subunits 2*, 13 + 16 and 5 + 10 were found in world wheat cultivars. In Slovak wheat varieties were analysed subunits 0, 7 + 9 and 5 + 10, 2 + 12. The HMW subunits 0, 7 + 8 with Glu-score 4 were determined in Triticum durum DESF. Three electrophoretical profile groups of different HMW glutenin subunits were found in Triticum turgidum L. and Triticum polonicum L. and six electrophoretical profile groups were determined in Triticum spelta L. The verified correlations between bread-making quality and specific HMW subunits of glutenin can be utilised by wheat breeders using SDS-PAGE of proteins as a screening test for the prediction of bread-making quality of wheat.

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Gluten index compared with SDS-sedimentation volume for early generation selection for gluten strength in durum wheat

Canadian Journal of Plant Science ◽

10.4141/cjps09035 ◽

2010 ◽

Vol 90 (1) ◽

pp. 1-11 ◽

Cited By ~ 18

Author(s):

F R Clarke ◽

J M Clarke ◽

N A Ames ◽

R E Knox ◽

R J Ross

Keyword(s):

Durum Wheat ◽

Protein Concentration ◽

Triticum Turgidum ◽

Sodium Dodecyl ◽

Field Trials ◽

Gluten Strength ◽

Sedimentation Volume ◽

Selection For ◽

Highly Correlated ◽

Gluten Index

Gluten strength is an important end-use quality factor in durum wheat [Triticum turgidum L. ssp. durum (Desf.) Husn.], affecting pasta manufacture and cooking quality. The objective of this research was to determine the inheritance and heritability of gluten index in comparison with the widely used SDS-sedimentation (sodium dodecyl sulphate) technique for selection for gluten strength. Seven durum populations were grown in replicated, multi-location, multi-year field trials in Saskatchewan, Canada, during the period 1995 to 2002. Gluten index and SDS-sedimentation volume were determined on all plots after harvest. Both traits were affected by genotype, and to a lesser extent by year or location. Genotype environmental interactions were generally minor. The majority of genotypes in each population had similar relative ranking for gluten index and SDS-sedimentation volume in each environment. Both traits were highly heritable, ranging from 0.80 to 0.97, and both were complexly inherited with estimates of effective factors ranging from 3 to 21. Gluten index and SDS-sedimentation volume were highly correlated, indicating that they are measuring similar aspects of gluten strength. SDS-sedimentation volume was positively associated with protein concentration (r2 = 0.52), but gluten index was not. Therefore, gluten index would be more desirable than SDS-sedimentation volume for use in selection where there are within-trial protein concentration trends.Key words: Gluten strength, SDS-sedimentation, gluten index, heritability, inheritance

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Molecular mapping of QTLs for gluten strength as measured by sedimentation volume and mixograph in durum wheat (Triticum turgidum L. ssp durum)

Journal of Cereal Science ◽

10.1016/j.jcs.2009.01.001 ◽

2009 ◽

Vol 49 (3) ◽

pp. 378-386 ◽

Cited By ~ 39

Author(s):

R.M. Patil ◽

M.D. Oak ◽

S.A. Tamhankar ◽

V.S. Rao

Keyword(s):

Durum Wheat ◽

Molecular Mapping ◽

Triticum Turgidum ◽

Gluten Strength ◽

Sedimentation Volume

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Relationships between the HMW- and LMW-glutenin subunits and SDS-sedimentation volume in Spanish hulled wheat lines

Czech Journal of Genetics and Plant Breeding ◽

10.17221/8/2008-cjgpb ◽

2008 ◽

Vol 44 (No. 3) ◽

pp. 114-117 ◽

Cited By ~ 3

Author(s):

L. Caballero ◽

L.M. Martín ◽

J.B. Alvarez

Keyword(s):

Protein Composition ◽

Quality Traits ◽

Glutenin Subunits ◽

Hulled Wheat ◽

Hulled Wheats ◽

Endosperm Storage Protein ◽

Wide Range ◽

Lmw Glutenin Subunits ◽

Sedimentation Volume ◽

Wheat Lines

Emmer and spelt are two hulled wheats that were widely grown in Spain until the latter 1960s. Twenty-nine emmer and twenty-six spelt lines obtained from Spanish accessions of these hulled wheats were analysed for quality traits and endosperm storage protein composition. The results showed a wide range of variability in these traits. Likewise, a certain association between some alleles of these proteins and the SDS-sedimentation volume has been detected.

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