scholarly journals The availability of DTPA extracted heavy metals during laboratory incubation of contaminated soils with glucose amendments

2011 ◽  
Vol 48 (No. 12) ◽  
pp. 536-542
Author(s):  
G. Mühlbachová

The laboratory incubation with glucose treatment was carried out in order to estimate the possible effects of increased microbial activity on heavy metal availability. The soils from vicinity of a&nbsp;lead smelter operating for more than 200 years were used for the experiment. The DTPA-extractable heavy metal contents increased after glucose addition and mostly reached the highest values the second day of the incubation. The comparative study, where the chloroform fumigation was used prior to the incubation in order to decrease the microbial activities, showed especially from second day of incubation significantly lower DTPA-extractable metal contents compared to non-fumigated treatments. The interactions among the maximum possible availability of DTPA-extractable heavy metal fractions and native soil microbial characteristics were studied in differently contaminated arable and grassland soils. Irrespective of different heavy metal contents in soils, significant correlations were found among the maximum percentage increase of DTPA-extractable Pb and Cd and the ratio Bc/TOC and metabolic quotient (qCO<sub>2</sub>) which may be a&nbsp;result of the important role of organic matter and microbial characteristics in soils on the heavy metal availability.

2011 ◽  
Vol 51 (No. 1) ◽  
pp. 26-33 ◽  
Author(s):  
G. Mühlbachová ◽  
T. Šimon ◽  
M. Pechová

The relationships among soil microbial biomass, pH and available of heavy metal fractions were evaluated in longterm contaminated soils during an incubation experiment with the amendment of zeolite (natural clinoptilolite) and the subsequent addition of glucose. The values of pH after the addition of glucose decreased during the first day of incubation approximately at about one unit and corresponded with the maximum increase of microbial biomass. The available heavy metal contents extracted by H<sub>2</sub>O, 1 mol/l NH<sub>4</sub>NO<sub>3</sub> and 0.005 mol/l DTPA increased during the first two days of incubation. Only a few significant relationships were found between the available metal contents and pH or microbial biomass. This fact could be ascribed to the different dynamics of the microbial biomass, pH and metal availability after glucose addition, when the highest metal contents during the incubation were usually reached one day later in respect to the greatest changes of pH and microbial activity. In comparison to soils without zeolite addition, the variants with natural clinoptilolite showed lower heavy metal contents in all used extractants with the exception of Cd which in H<sub>2</sub>O extracts tended to increase.


2011 ◽  
Vol 49 (No. 12) ◽  
pp. 536-541 ◽  
Author(s):  
G. Mühlbachová ◽  
T. Šimon

A&nbsp;laboratory incubation experiment with zeolite and glucose was performed to evaluate the effects of zeolite amendment in heavy metal contaminated soils from two smelter areas on some microbial characteristics [Kremikovtzi (K1, K2) in Bulgariaand Př&iacute;bram (P1, P2) in the CzechRepublic]. The content of microbial biomass showed a&nbsp;tendency to decrease in Kremikovtzi soils whereas in Př&iacute;bram soils no significant effects were found after zeolite amendment. Respiratory activity and metabolic quotient (qCO2) decreased on the second and third day in Kremikovtzi soils amended with zeolite, no effects were observed in Př&iacute;bram soils. Heavy metals decreased the content of microbial biomass in Kremikovtzi soils whereas the contaminated soil from Př&iacute;bram area had the highest microbial biomass compared to non-contaminated soil during incubation, probably due to lower mineralization of carbon. The respiratory activity did not show any significant effects of zeolites on the evolution of CO2&nbsp;and qCO2&nbsp;in heavy metal contaminated Př&iacute;bram soil. The respiratory activity in non-contaminated Př&iacute;bram soil remained during the experiment lower in comparison to contaminated one, however the addition of zeolite increased qCO2.


2019 ◽  
Vol 17 (3) ◽  
pp. 288-294
Author(s):  
Md Akhter Hossain Chowdhury ◽  
Tanzin Chowdhury ◽  
Md Arifur Rahman

Heavy metal accumulation in environmental compartments is a potential risk to the living system because of their uptake by plants and subsequent introduction into the food chain. A study was carried out to investigate the heavy metal contents in industrially contaminated soils collected from six different locations of Dhaka and Mymensingh districts and their effects on two important vegetables namely tomato and cabbage. Pot experiment was conducted using contaminated soils at the net house of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh following completely randomized design (CRD) with three replicates. The higher level of heavy metal contents was found in the soil samples of Hajaribag and Dhaka Export Processing Zone (DEPZ). The highest Ni, Cd, Cr, Cu and Fe contents were 59.45, 18.79, 67.57, 40.81 and 1619.61 µg g−1 which were much above the recommended level except Cu contents. The highest yield of vegetables was obtained grown in Maskanda soil of Mymensingh district and the lowest from DEPZ soil of Dhaka. The highest Ni, Cr and Fe contents were 8.91, 7.22, 419.65 µg g−1, respectively in tomato fruits grown in the soil of Hajaribag whereas the highest Cu content (3.38 µg g−1) was obtained from Seedstore soil, Mymensingh and highest Cd content (2.88 µg g−1) was from Mitford ghat soil, Dhaka. In cabbage, the highest Ni (17.52 µg g−1) and Fe (411.25 µg g−1) contents were found in the soils of DEPZ whereas the highest Cr (9.17 µg g−1), Cd (3.52 µg g−1) and Cu (8.51 µg g−1) were obtained in the plants grown in the soils of Hajaribag, Mitford ghat and Maskanda, respectively. Concentrations of all the tested heavy metals except Cu in both vegetables were above the maximum allowable limit prescribed by the World Health Organization. Among the metals, the accumulation of Ni was found as higher amount (0.39 and 0.71 for tomato and cabbage, respectively) based on plant concentration factor or transfer factor. The results showed a positive correlation between concentration of the metals present in soils and in vegetables and the highest correlation was found with Cr in tomato and Fe in cabbage. However, both the soils and grown vegetables were consistently observed to pose a risk to human health. So, it can be recommended that government should take necessary action so that heavy metals used in the industries cannot come into the nearby agricultural field to ensure food safety as well as food security. J Bangladesh Agril Univ 17(3): 288–294, 2019


1995 ◽  
Vol 32 (12) ◽  
pp. 29-34 ◽  
Author(s):  
Christina Siebe

The relationship between three different heavy metal fractions in soils irrigated for long periods of time with untreated wastewater from Mexico City and the metal uptake by alfalfa (Medicago sativa) was studied in order to evaluate actual accumulation levels in soils, as well as to establish which extracting solution characterizes most precisely the availability of selected heavy metals to plants. Cd, Pb, Cu and Zn were analyzed in aqua regia, 1 M NH4NO3 and water extracts from 50 soil samples and correlated with total contents in alfalfa tissue collected at the same sites. Heavy metal contents of the 3 fractions increase significantly with irrigation time, but European tolerance limits are slightly exceeded only for Cd at some sites. Plant uptake of Cd and Pb also increases the longer the soils have been irrigated and correlates best with aqua regia extracts from soils.


Biochar ◽  
2021 ◽  
Author(s):  
Nahid Azadi ◽  
Fayez Raiesi

AbstractSoil amendment with biochar alleviates the toxic effects of heavy metals on microbial functions in single-metal contaminated soils. Yet, it is unclear how biochar application would improve microbial activity and enzymatic activity in soils co-polluted with toxic metals. The present research aimed at determining the response of microbial and biochemical attributes to addition of sugarcane bagasse biochar (SCB) in cadmium (Cd)-lead (Pb) co-contaminated soils. SCBs (400 and 600 °C) decreased the available concentrations of Cd and Pb, increased organic carbon (OC) and dissolved organic carbon (DOC) contents in soil. The decrease of metal availability was greater with 600 °C SCB than with 400 °C SCB, and metal immobilization was greater for Cd (16%) than for Pb (12%) in co-spiked soils amended with low-temperature SCB. Biochar application improved microbial activity and biomass, and enzymatic activity in the soils co-spiked with metals, but these positive impacts of SCB were less pronounced in the co-spiked soils than in the single-spiked soils. SCB decreased the adverse impacts of heavy metals on soil properties largely through the enhanced labile C for microbial assimilation and partly through the immobilization of metals. Redundancy analysis further confirmed that soil OC was overwhelmingly the dominant driver of changes in the properties and quality of contaminated soils amended with SCB. The promotion of soil microbial quality by the low-temperature SCB was greater than by high-temperature SCB, due to its higher labile C fraction. Our findings showed that SCB at lower temperatures could be applied to metal co-polluted soils to mitigate the combined effects of metal stresses on microbial and biochemical functions.


2015 ◽  
Vol 74 (7) ◽  
pp. 5849-5858 ◽  
Author(s):  
Bouchra Kennou ◽  
Mohamed El Meray ◽  
Abderrahmane Romane ◽  
Youssef Arjouni

1996 ◽  
Vol 42 (6) ◽  
pp. 593-603 ◽  
Author(s):  
T. M. Roane ◽  
S. T. Kellogg

Heavy metal pollution is a principle source of environmental contamination. We analyzed heavy metal impacted soil microbial communities and found that, in general, although lead adversely affected biomass, metabolic activity, and diversity, autochthonous lead- and cadmium-resistant isolates were found. In several metal-stressed soils, the microbial community consisted of two populations, either resistant or sensitive to lead. Additionally, a lead-resistant isolate was isolated from a control soil with no known previous exposure to lead, suggesting widespread lead resistance. Lead-resistant genera isolated included Pseudomonas, Bacillus, Corynebacterium, and Enterobacter species. Plasmids, ranging from 5 to 260 kb, were not detected through standard purifications from lead-resistant isolates. Positive correlations existed between antibiotic resistance and isolation habitat for lead-resistant strains, microbial metabolic activity and soil type, soluble lead concentration and microbial diversity, and arsenic concentration and total or viable cell concentrations.Key words: heavy metal, lead, cadmium, soil bacteria, stress, remediation.


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