Clary Sage Cultivation and Mycorrhizal Inoculation Influence the Rhizosphere Fungal Community of an Aged Trace-Element Polluted Soil

Soil fungal communities play a central role in natural systems and agroecosystems. As such, they have attracted significant research interest. However, the fungal microbiota of aromatic plants, such as clary sage (Salvia sclarea L.), remain unexplored. This is especially the case in trace element (TE)-polluted conditions and within the framework of phytomanagement approaches. The presence of high concentrations of TEs in soils can negatively affect not only microbial diversity and community composition but also plant establishment and growth. Hence, the objective of this study is to investigate the soil fungal and arbuscular mycorrhizal fungi (AMF) community composition and their changes over time in TE-polluted soils in the vicinity of a former lead smelter and under the cultivation of clary sage. We used Illumina MiSeq amplicon sequencing to evaluate the effects of in situ clary sage cultivation over two successive years, combined or not with exogenous AMF inoculation, on the rhizospheric soil and root fungal communities. We obtained 1239 and 569 fungal amplicon sequence variants (ASV), respectively, in the rhizospheric soil and roots of S. sclarea under TE-polluted conditions. Remarkably, 69 AMF species were detected at our experimental site, belonging to 12 AMF genera. Furthermore, the inoculation treatment significantly shaped the fungal communities in soil and increased the number of AMF ASVs in clary sage roots. In addition, clary sage cultivation over successive years could be one of the explanatory parameters for the inter-annual variation in both fungal and AMF communities in the soil and root biotopes. Our data provide new insights on fungal and AMF communities in the rhizospheric soil and roots of an aromatic plant, clary sage, grown in TE-polluted agricultural soil.

Activity and Diversity of Microorganisms in Root Zone of Plant Species Spontaneously Inhabiting Smelter Waste Piles

The aim was to assess plant driven changes in the activity and diversity of microorganisms in the top layer of the zinc and lead smelter waste piles. The study sites comprised two types (flotation waste—FW and slag waste—SW) of smelter waste deposits in Piekary Slaskie, Poland. Cadmium, zinc, lead, and arsenic contents in these technosols were extremely high. The root zone of 8 spontaneous plant species (FW—Thymus serpyllum, Silene vulgaris, Solidago virgaurea, Echium vulgare, and Rumex acetosa; and SW—Verbascum thapsus; Solidago gigantea, Eupatorium cannabinum) and barren areas of each waste deposit were sampled. We observed a significant difference in microbial characteristics attributed to different plant species. The enzymatic activity was mostly driven by plant-microbial interactions and it was significantly greater in soil affected by plants than in bulk soil. Furthermore, as it was revealed by BIOLOG Ecoplate analysis, microorganisms inhabiting barren areas of the waste piles rely on significantly different sources of carbon than those found in the zone affected by spontaneous plants. Among phyla, Actinobacteriota were the most abundant, contributing to at least 25% of the total abundance. Bacteria belonging to Blastococcus genera were the most abundant with the substantial contribution of Nocardioides and Pseudonocardia, especially in the root zone. The contribution of unclassified bacteria was high—up to 38% of the total abundance. This demonstrates the unique character of bacterial communities in the smelter waste.

Reconstruction of 16th–17th Century Lead Smelting Processes on the Basis of Slag Properties: A Case Study from Sławków, Poland

The study focuses on the reconstruction of the technological process in the 16th–17th century lead smelter in Sławków based on chemical and petrographic analyzes of slags. There are three main types of material at the landfill: glassy, crystalline, and weathered. Glassy slags are made of amorphous phase in which crystals of pyroxene, willemite, olivine, wüstite, and lead oxide appear. Crystalline slags are composed of wollastonite, rankinite, melilite, anorthite, quartz, and Fe oxides. Weathered slags have a composition similar to glassy slags, but they also contain secondary phases: anglesite and cerussite. Chemical analyzes confirmed that the smelter used sulphide ores, which were roasted, and the main addition to the charge was quartz sand. The smelting process took place in a brick-built furnace, under reducing conditions, with varied oxygen fugacity ranging from WM to MH buffer. The slag characteristics show a knowledge of the workers in the field of smelting methods. The addition of SiO2 allowed for the binding of elements that could contaminate the obtained lead, and at the same time, the low melting point of the material (1150 °C) and the melt viscosity (logη = 1.34 for 1150 °C) was maintained, enabling the effective separation of liquid lead.

Great Mining Camps of Canada 8. The Bathurst Mining Camp, New Brunswick, Part 2: Mining History and Contributions to Society

In the Bathurst Mining Camp (BMC), 12 of the 45 known massive sulphide deposits were mined between 1957 and 2013; one was mined for iron prior to 1950, whereas three others had development work but no production. Eleven of the deposits were mined for base metals for a total production of approximately 179 Mt, with an average grade of 3.12% Pb, 7.91% Zn, 0.47% Cu, and 93.9 g/t Ag. The other deposit was solely mined for gold, present in gossan above massive sulphide, producing approximately one million tonnes grading 1.79 g/t Au. Three of the 11 mined base-metal deposits also had a gossan cap, from which gold was extracted. In 2012, the value of production from the Bathurst Mining Camp exceeded $670 million and accounted for 58 percent of total mineral production in New Brunswick.Base-metal production started in the BMC in 1957 from deposits at Heath Steele Mines, followed by Wedge in 1962, Brunswick No. 12 in 1964, Brunswick No. 6 in 1965, Caribou in 1970, Murray Brook, Stratmat Boundary and Stratmat N-5 in 1989, Captain North Extension in 1990, and lastly, Half Mile Lake in 2012. The only mine in continuous production for most of this time was Brunswick No. 12. During its 49-year lifetime (1964–2013), it produced 136,643,367 tonnes of ore grading 3.44% Pb, 8.74% Zn, 0.37% Cu, and 102.2 g/t Ag, making it one of the largest underground base-metal mines in the world.The BMC remains important to New Brunswick and Canada because of its contributions to economic development, environmental measures, infrastructure, mining innovations, and society in general. The economic value of metals recovered from Brunswick No. 12 alone, in today’s prices exceeds $46 billion. Adding to this figure is production from the other mines in the BMC, along with money injected into the local economy from annual exploration expenditures (100s of $1000s per year) over 60 years. Several environmental measures were initiated in the BMC, including the requirement to be clean shaven and carry a portable respirator (now applied to all mines in Canada); ways to treat acid mine drainage and the thiosalt problem that comes from the milling process; and pioneering studies to develop and install streamside-incubation boxes for Atlantic Salmon eggs in the Nepisiguit River, which boosted survival rates to over 90%. Regarding infrastructure, provincial highways 180 and 430 would not exist if not for the discovery of the BMC; nor would the lead smelter and deep-water port at Belledune. Mining innovations are too numerous to list in this summary, so the reader is referred to the main text. Regarding social effects, the new opportunities, new wealth, and training provided by the mineral industry dramatically changed the living standards and social fabric of northern New Brunswick. What had been a largely poor, rural society, mostly dependent upon the fishing and forestry industries, became a thriving modern community. Also, untold numbers of engineers, geologists, miners, and prospectors `cut their teeth’ in the BMC, and many of them have gone on to make their mark in other parts of Canada and the world.

Cadmium Is Associated with Type 2 Diabetes in a Superfund Site Lead Smelter Community in Dallas, Texas

Objective: To test the hypothesis that cadmium (Cd) exposure is associated with type 2 diabetes mellitus (T2DM). Materials and Methods: A two-phase health screening (physical examination and laboratory tests) was conducted in a lead smelter community following a Superfund Cleanup. Participants were African Americans aged >19 years to <89 years. Multiple logistic regression was used to analyze T2DM regressed on blood Cd level and covariates: body mass index (BMI), heavy metals (Ar, Cd, Hg, Pb), duration of residence, age, smoking status, and sex. Results: Of 875 subjects environmentally exposed to Cd, 55 were occupationally exposed to by-products of lead smelting and 820 were community residents. In addition, 109 T2DM individuals lived in the community for an average of 21.0 years, and 766 non-T2DM individuals for 19.0 years. T2DM individuals (70.3%) were >50 years old. Blood Cd levels were higher among T2DM subjects (p < 0.006) compared to non-T2DM individuals. Logistic regression of T2DM status identified significant predictors: Cd level (OR = 1.85; 95% CI: 1.14–2.99, p < 0.01), age >50 years (OR = 3.10; 95% CI: 1.91–5.02, p < 0.0001), and BMI (OR = 1.07; CI: 1.04–1.09, 0.0001). In meta-analysis of 12 prior studies and this one, T2DM risk was OR = 1.09 (95% CI: 1.03–1.15, p < 0.004) fixed effects and 1.22 (95% CI: 1.04–1.44, p < 0.02) random effects. Discussion: Chronic environmental Cd exposure was associated with T2DM in a smelter community, controlling for covariates. T2DM onset <50 years was significantly associated with Cd exposure, but >50 years was not. Meta-analysis suggests that Cd exposure is associated with a small, but significant increased risk for T2DM. Available data suggest Cd exposure is associated with an increased propensity to increased insulin resistance.