scholarly journals Associations between bovine lactoferrin gene polymorphism and somatic cell count in milk

2012 ◽  
Vol 51 (No. 1) ◽  
pp. 14-20 ◽  
Author(s):  
K. Wojdak-Maksymiec ◽  
M. Kmiec ◽  
J. Ziemak
Keyword(s):  
Gene Polymorphism ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Bovine Lactoferrin ◽  
Logarithmic Scale ◽  
Black And White ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Lactoferrin Gene

The study included 124 Polish Black-and-White dairy cows of various share of the Holstein-Friesian (HF) breed. Lactoferrin (LTF) gene polymorphism was obtained with PCR-RFLP method using EcoRI enzyme. Two alleles of LTF, A and B, were found in the studied population. Their frequencies were 67.74% and 32.56%, respectively. The alleles controlled the occurrence of three genotypes: AA, BB and AB, of frequencies equal to 37.90%, 2.42% and 59.68%, respectively. It was established that statistically significant associations exist between the somatic cell count (SCC) and LTF genotype, lactation month and parity as well as the HF gene share. No significant association was found between somatic cell count and season. The highest somatic cell count (transformed to a logarithmic scale) was found in milk of the AB genotype, whereas the lowest one was found in cows of the AA genotype.

CATHL2 gene polymorphism in relation to production traits in Holstein-Friesian cows

2019 ◽  
Vol 15 (2) ◽  
pp. 23-30
Author(s):  
Sonia Hiller ◽  
Inga Kowalewska-Łuczak ◽  
Ewa Czerniawska-Piątkowska
Keyword(s):  
Statistical Analysis ◽  
Gene Polymorphism ◽  
Somatic Cell Count ◽  
The Other ◽  
Production Traits ◽  
Black And White ◽  
Potential Association ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Friesian Cows

The aim of this study was to identify SNPs mutations in the CATHL2 gene and determine their potential association with dairy performance traits in Polish Black-and-White Holstein-Friesian (phf) cows. Genotypes of individuals were identified by PCR-RFLP. The frequencies of CATHL2/DdeI alleles were C ‒ 0.693 and T ‒ 0.307, and for CATHL2/HhaI polymorphisms, G ‒ 0.763 and C ‒ 0.237. The statistical analysis showed that cows with the CC (CATHL2/DdeI) and CG (CATHL2/HhaI) genotype produced higher milk yield than the other cattle genotypes. In the case of CATHL2/DdeI and CATHL2/HhaI polymorphisms, the highest somatic cell count was found in heterozygous CT and CG cows.

scholarly journals Associations between microsatellite polymorphism within the macrophage expressed lysozyme (mLys) gene and milk Indices Properties in Polish Black-and-White Cows

2005 ◽  
Vol 53 (2) ◽  
pp. 99-106
Author(s):  
Małgorzata Walczak-Wójciak ◽  
Janusz Klupczyński ◽  
Jan Miciński ◽  
Martin Hošek
Keyword(s):  
Gene Polymorphism ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Production Capacity ◽  
Bacterial Count ◽  
Mineral Elements ◽  
Technological Properties ◽  
Black And White ◽  
Pathogenic Factors

The experiment was performed in the years 1998–2000 on two farms located near to Vistula Lowlands. The experimental materials were comprised of 52 daughters of bull Paran, among which 21 possessed the mLys-mic 7 allele and 31 – the mLys-mic 3 allele. The serum and whey bacteriolytic activity of the lysozyme, concentrations of selected mineral elements, technological properties of milk at successive the first lactation stages (30, 100, 150 and 200 days), somatic cell count and bacterial count were investigated in this study. The results obtained indicate that Lys-mic polymorphism has low suitability as a marker for milk production capacity in cows. The lysozyme gene, treated as a mastitis resistance factor, showed no effects on somatic cell count and bacterial count. Further investigations, conducted not only during the first lactation, but also during the next lactation in a herd threatened by a variety of pathogenic factors, would be carried to validate this Lys-mic gene polymorphism on mastitis resistance.

Lactoferrin gene variants, their expression in the udder and mastitis susceptibility in dairy cattle

2015 ◽  
Vol 55 (8) ◽  
pp. 999 ◽  
Author(s):  
Adrianna Pawlik ◽  
Grażyna Sender ◽  
Magdalena Sobczyńska ◽  
Agnieszka Korwin-Kossakowska ◽  
Henryka Lassa ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Holstein Friesian ◽  
Lactoferrin Gene ◽  
Estimated Breeding Values ◽  
Mastitis Susceptibility

Lactoferrin gene (LF) is regarded as one of the potential markers of mastitis susceptibility/resistance in dairy cattle. The study’s aim was therefore, to investigate the feasibility of two single nucleotide polymorphisms (SNP), placed in the 5′-flanking region and 3′-untranslated region of the LF gene, to serve as mastitis markers. The associations between these SNP and the expression of LF, both on mRNA and protein level, were estimated in the milk of Polish Holstein-Friesian cows. The relationships between polymorphisms and cows’ estimated breeding values (EBV) for somatic cell count were also calculated. It was shown that both polymorphisms have a significant impact on lactoferrin content in milk, and that LF+32 SNP is associated with the cow’s EBV for somatic cell count. No association between SNP chosen for the study and lactoferrin mRNA abundance in milk somatic cells was observed. We propose LF+32 SNP for a molecular marker of mastitis resistance in dairy cows.

scholarly journals Some factors influencing milk somatic cell count of Holstein Friesian and Brown Swiss cows under the Mediterranean climatic conditions

2009 ◽  
Vol 52 (2) ◽  
pp. 124-133 ◽  
Author(s):  
A. Koç ◽  
K. Kizilkaya
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Repeated Measures ◽  
Somatic Cell Count ◽  
Control Program ◽  
Resistance Mechanisms ◽  
Climatic Conditions ◽  
Brown Swiss ◽  
Holstein Friesian ◽  
The Mediterranean

Abstract. The aim of this study was to determine the influencing factors on somatic cell count (SCC) in the milk of Holstein Friesian (HF) and Brown Swiss (BS) cows raised on three dairy farms under the Mediterranean climatic conditions in Turkey. For a two-year period, farms were visited monthly to measure daily milk yield (DMY) and collect milk samples from each cow during the morning and evening milking. Total of 1 429 SCC readings from 67 HF and 16 BS cows were analyzed by using repeated measures. Breed (P<0.01), lactation number (LN) (P<0.01), milking time (MT) (P<0.05), lactation month (LM) (P<0.01) and the interactions of breed-LM (P<0.05), herd-LM (P<0.01) and LN-LM (P<0.01) effects on SCC were statistically significant as did the co-variable DMY effect (P<0.01). The effect of herd and herd-MT interactions on SCC were statistically insignificant (P>0.05). The SCC means for BS and HF cows were 5.464±0.060 (291 072 cells/ml) and 5.654±0.029 (450 817 cells/ml), respectively. The SCC mean for morning milking (338 065 cells/ml) was 25 850 cells/ml lower than that of evening milking. The LN4 had the highest SCC mean (490 908 cells/ml) and was statistically different from those of LN1 (P<0.01) and LN2 (P<0.05). The SCC level was the highest in the first LM (601 174 cells/ml) and this level then decreased in the later months. Possible reasons for the difference in SCC means between the breeds are different resistance mechanisms against mastitis, different morphological conformations of udders and different milk yields between these two breeds. The insignificant differences found among herds show the similarities of management, milking hygiene and barn conditions. In order to decrease SCC in milk and increase udder health, some precautions need to be taken like improving milking management, hygiene and barn conditions, milking the cow at uniform intervals, feeding the cows after the milking and applying a mastitis control program.

The MUC 1 gene polymorphism is not associated with milk productive traits and somatic cell count in Jersey cattle

2019 ◽  
Vol 50 (2) ◽  
pp. 188-189
Author(s):  
Lucas Vargas ◽  
Daniel Duarte Silveira ◽  
Rogério Fôlha Bermudes ◽  
Heden Luiz Marques Moreira ◽  
Anibal Eugênio Vercesi Filho ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Jersey Cattle ◽  
Productive Traits

An attempt to develop a method for determining the typical chemical composition of the milk of Polish Holstein-Friesian cows – a proposal

2019 ◽  
Vol 15 (3) ◽  
pp. 9-21
Author(s):  
Piotr Guliński ◽  
Anna Kłopotowska
Keyword(s):  
Chemical Composition ◽  
Somatic Cell ◽  
Cell Count ◽  
Milk Yield ◽  
Somatic Cell Count ◽  
Scientific Publications ◽  
Lactation Stage ◽  
Holstein Friesian ◽  
Calving Season ◽  
The Impact

The aim of this paper is to develop a method for determining the typical chemical composition of the milk of Polish Holstein-Friesian (PHF) cows. The paper uses data collected from 1329 test-day milking records from 20 herds of PHF dairy cattle in Sokołów County, from 2009 to 2015. The effect of the following factors on the chemical composition of milk was determined: lactation stage (15 one-month stages); age of cows (lactations 1, 2, 3–4, and 5–7); genotype (share of PHF breed: less than 50%, 50–75%, 75–82.5% and more than 82.5%); somatic cell count (SCC) in 1 ml of milk (in thousands: 0–200, 200–400, 400–1000 and more than 1000); feeding level (fat to protein (F/P) ratio): ≤1.0, 1.0–1.4, 1.4–1.7 and >1.7); calving season (autumn/winter, spring/summer) and daily milk yield (milk yield in kg: ≤15, 15–25, 25–35 and >35). Nutrition and udder health status were found to be the main factors influencing the chemical composition of milk. For selected cows with optimally balanced feed rations (F/P ratio in milk from 1.1 to 1.4) and a low somatic cell count (SCC ≤200,000/ml), daily yield was the main factor affecting the chemical composition of the milk. It was also concluded that government and scientific publications on the PHF breed should take into account the impact of the F/P ratio, SCC and yield of milk on its composition.

RELATIONSHIPS BETWEEN SPEED OF MILKING AND SOMATIC CELL COUNT AND PRODUCTION IN HOLSTEINS

1983 ◽  
Vol 63 (4) ◽  
pp. 781-789 ◽  
Author(s):  
R. K. MOORE ◽  
J. E. MOXLEY ◽  
B. W. KENNEDY ◽  
E. B. BURNSIDE
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Milk Yield ◽  
Somatic Cell Count ◽  
Genetic Correlations ◽  
Heritability Estimate ◽  
Phenotypic Correlations ◽  
Cell Counts ◽  
Holstein Friesian ◽  
The Relationship

Milking speed data were obtained for 2604 Holstein-Friesian cows, identified by sire, in test herds located in Quebec and Ontario. Milk samples were collected from each cow and analyzed for somatic cell count. Completed or projected lactation production records were available for this sample of cows. Two-minute yield and total milking time were adjusted for the effect of milk yield at sampling and the raw cell counts were transformed to the natural log scale. Sire and error variances were obtained by maximum likelihood (ML) methods and used to estimate heritabilities of and correlations between traits. The heritability estimate for the adjusted 2-min. yield, 0.23, was higher than that for the adjusted total milking time (0.13), with the estimates for the two unadjusted measures being intermediate (0.18). The phenotypic correlations between milking speed and somatic cell count were small. However, there were two distinct linear phases to the relationship between the adjusted 2-min yield and cell count. Small but significant phenotypic correlations were observed between unadjusted measures of milking speed and lactation production (0.11–0.22); however, correlations were not significant when adjustments were made for the milk yield at sampling. Genetic correlations between milking speed and somatic cell count were moderate to large and indicated an antagonistic relationship between faster milking speed and cell count. Also, the genetic correlations suggested some antagonism between increasing 2-min yield and lactation production, while the relationship between lactation traits and milking time was small. Key words: Milking speed, somatic cell count, correlations, heritabilities, Holsteins

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