scholarly journals Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy

2012 ◽  
Vol 49 (No. 7) ◽  
pp. 225-236 ◽  
Author(s):  
T. Amemori ◽  
L. Matlova ◽  
O. A Fischer ◽  
W. Y Ayele ◽  
M. Machackova ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Laparoscopic Approach ◽  
Ileocecal Valve ◽  
Mycobacterium Avium Subsp ◽  
Sample Collection ◽  
Mesenteric Lymph Nodes ◽  
Laparoscopic Biopsy ◽  
Mycobacterium Avium Subsp Paratuberculosis

The gastrointestinal tract (GIT) is a major target for Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) in cattle. Culture examination was achieved in tissue samples obtained from 10 different regions of the GIT (proximal and distal parts of the duodenum, proximal, middle and distal parts of the jejunum, proximal and distal parts of the ileum, the ileocecal valve, the caecum and the rectum) and their adjacent lymph nodes. The culture results were statistically analysed to elucidate the distribution of M. a. paratuberculosis in the GIT. A total of 63 cows older than 24 months were diagnosed with paratuberculosis by faecal and tissue cultures. The better detection rate of M. a. paratuberculosis was found in the mucosae from the jejunum to the ileocecal valve and in the lymph nodes from the jejunum to the caecum. The mean number of colony forming units (CFU) in the mucosae and the lymph nodes of the distal jejunum and the proximal ileum was significantly higher than that in the mucosae of the duodenum, the caecum and the rectum, and in the lymph nodes of the duodenum and the rectum, respectively (p < 0.05). Laparoscopic biopsy attempted out on 4 animals to test its potential use for sample collection from the statistically optimal mesenteric lymph nodes; but resulted in an abortive attempt because these targets were encircled by the intestines, the pressure of which complicated the laparoscopic approach.

scholarly journals Incidence of Mycobacterium avium Subsp. paratuberculosis (MAP) Infection in slaughtered buffaloes (Bubalus bubalis) of Malwa Region of Madhya Pradesh

2017 ◽  
Vol 13 (02) ◽  
Author(s):  
G. P. Jatav ◽  
U. K. Garg ◽  
Supriya Shukla ◽  
Daljeet Chhabra ◽  
A. K. Jayraw ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Bubalus Bubalis ◽  
Madhya Pradesh ◽  
Mycobacterium Avium Subsp ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Gross Lesions ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Infection Study

The study was undertaken to assess the incidence of subclinical and clinical paratuberculosis in slaughtered buffaloes of Malwa region of Madhya Pradesh. They were unproductive buffaloes (1- 10 years old) slaughtered in Mhow and Indore examined for subclinical and clinical Mycobacterium avium subsp. paratuberculosis (MAP) infection. Study of gross lesions of intestine and respective mesenteric lymph nodes (MLNs) showed overall 87.33% (131/150) incidence of paratuberculosis. Out of these 131 cases of paratuberculosis, 18.21% (24/131) buffaloes suffered from subclinical and 81.68% (107/131) from clinical paratuberculosis, whereas impression smear examination revealed clinical and subclinical paratuberculosis up to 25.77 and 74.33% in intestine and 13.51 and 86.49% in MLNs, respectively.

Ultrastructural studies of bovine paratuberculosis (Johne's Disease)

2015 ◽  
Vol 15 (2) ◽  
pp. 61-65
Author(s):  
T Batbayar ◽  
S Andrei ◽  
Ch Tungalag
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Giant Cells ◽  
Mycobacterium Avium Subsp ◽  
Epithelioid Cells ◽  
Mesenteric Lymph Nodes ◽  
Ultrastructural Studies ◽  
Mesenteric Lymph ◽  
Agricultural Sciences ◽  
Mycobacterium Avium Subsp Paratuberculosis

In the present study, we report the ultrastructural morphologic features of infected macrophages, epithelioid cells, Langhans' giant cells and a more detailed impression of the interaction between macrophages and engulfed bacteria in ileum and mesenteric lymph nodes of cows naturally infected with Mycobacterium avium subsp. paratuberculosis (MAP).Mongolian Journal of Agricultural Sciences Vol.15(2) 2015; 61-65 

Mycobacterium avium subsp. avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic

2014 ◽  
Vol 77 (1) ◽  
pp. 141-144 ◽  
Author(s):  
PETR KRIZ ◽  
MARIJA KAEVSKA ◽  
IVA SLANA ◽  
IVA BARTEJSOVA ◽  
IVO PAVLIK
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Mesenteric Lymph Nodes ◽  
Tissue Samples ◽  
The Czech Republic ◽  
Mesenteric Lymph ◽  
Qpcr Method ◽  
Pig Carcasses ◽  
Slaughtered Pigs

This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB)–like lesions from 17 pig carcasses, as well as samples of head and mesenteric lymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (102 to 103 cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantification of M. a. avium in pigs for the purposes of foodborne risk assessment.

Prevalence of Mycobacterium avium subsp. paratuberculosis in Ileocecal Lymph Nodes and on Hides and Carcasses from Cull Cows and Fed Cattle at Commercial Beef Processing Plants in the United States†

2009 ◽  
Vol 72 (7) ◽  
pp. 1457-1462 ◽  
Author(s):  
J. E. WELLS ◽  
J. M. BOSILEVAC ◽  
N. KALCHAYANAND ◽  
T. M. ARTHUR ◽  
S. D. SHACKELFORD ◽  
...  
Keyword(s):  
United States ◽  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
The United States ◽  
Mycobacterium Avium Subsp ◽  
Culture Techniques ◽  
Beef Processing ◽  
Processing Plants ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Cull Cow

Clinical associations between Crohn's disease in humans and Mycobacterium avium subsp. paratuberculosis (MAP) have been suggested but not confirmed. Cattle could be sources for MAP, but little information on MAP prevalence with beef has been reported. Samples of ileocecal lymph nodes and swabs of hides and carcasses from 343 animals at cull cattle slaughtering facilities and 243 animals at fed cattle slaughtering facilities across the United States were analyzed for the presence of MAP. Amplification of genetic sequences detected MAP DNA predominantly on hides and in lymph nodes of samples taken at both types of processing facilities. More than 34% of the cattle at cull cow slaughtering facilities had ileocecal lymph nodes that tested positive for MAP DNA. From these same cattle, hide prevalence was more than twofold greater than the prevalence in ileocecal lymph nodes, suggesting that cross-contamination could be occurring during transport and lairage. The prevalence of MAP DNA decreased during processing, and less than 11% of the carcasses tested positive after interventions in the cull cow processing facilities. Using standard double-decontamination and culture techniques, less than 1% of the postintervention carcasses tested positive for viable MAP at cull cow facilities. In samples from the facilities processing only fed cattle, MAP prevalence of 1% or less was detected for ileocecal lymph node, hide, and carcass samples, and viable MAP was not detected. Based on this study, fed cattle carcasses are unlikely sources of MAP, and carcasses at cull cow plants have only a slight risk for transmitting viable MAP, due to current interventions.

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