scholarly journals Incidence of Mycobacterium avium Subsp. paratuberculosis (MAP) Infection in slaughtered buffaloes (Bubalus bubalis) of Malwa Region of Madhya Pradesh

2017 ◽  
Vol 13 (02) ◽  
Author(s):  
G. P. Jatav ◽  
U. K. Garg ◽  
Supriya Shukla ◽  
Daljeet Chhabra ◽  
A. K. Jayraw ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Bubalus Bubalis ◽  
Madhya Pradesh ◽  
Mycobacterium Avium Subsp ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Gross Lesions ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Infection Study

The study was undertaken to assess the incidence of subclinical and clinical paratuberculosis in slaughtered buffaloes of Malwa region of Madhya Pradesh. They were unproductive buffaloes (1- 10 years old) slaughtered in Mhow and Indore examined for subclinical and clinical Mycobacterium avium subsp. paratuberculosis (MAP) infection. Study of gross lesions of intestine and respective mesenteric lymph nodes (MLNs) showed overall 87.33% (131/150) incidence of paratuberculosis. Out of these 131 cases of paratuberculosis, 18.21% (24/131) buffaloes suffered from subclinical and 81.68% (107/131) from clinical paratuberculosis, whereas impression smear examination revealed clinical and subclinical paratuberculosis up to 25.77 and 74.33% in intestine and 13.51 and 86.49% in MLNs, respectively.

Ultrastructural studies of bovine paratuberculosis (Johne's Disease)

2015 ◽  
Vol 15 (2) ◽  
pp. 61-65
Author(s):  
T Batbayar ◽  
S Andrei ◽  
Ch Tungalag
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Giant Cells ◽  
Mycobacterium Avium Subsp ◽  
Epithelioid Cells ◽  
Mesenteric Lymph Nodes ◽  
Ultrastructural Studies ◽  
Mesenteric Lymph ◽  
Agricultural Sciences ◽  
Mycobacterium Avium Subsp Paratuberculosis

In the present study, we report the ultrastructural morphologic features of infected macrophages, epithelioid cells, Langhans' giant cells and a more detailed impression of the interaction between macrophages and engulfed bacteria in ileum and mesenteric lymph nodes of cows naturally infected with Mycobacterium avium subsp. paratuberculosis (MAP).Mongolian Journal of Agricultural Sciences Vol.15(2) 2015; 61-65 

Mycobacterium avium subsp. avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic

2014 ◽  
Vol 77 (1) ◽  
pp. 141-144 ◽  
Author(s):  
PETR KRIZ ◽  
MARIJA KAEVSKA ◽  
IVA SLANA ◽  
IVA BARTEJSOVA ◽  
IVO PAVLIK
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Mesenteric Lymph Nodes ◽  
Tissue Samples ◽  
The Czech Republic ◽  
Mesenteric Lymph ◽  
Qpcr Method ◽  
Pig Carcasses ◽  
Slaughtered Pigs

This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB)–like lesions from 17 pig carcasses, as well as samples of head and mesenteric lymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (102 to 103 cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantification of M. a. avium in pigs for the purposes of foodborne risk assessment.

scholarly journals Paratuberculosis: new histopathological findings in red deer (Cervus elaphus) and fallow deer (Dama dama) in Chile

2017 ◽  
Vol 37 (7) ◽  
pp. 749-753 ◽  
Author(s):  
Esteban Reyes Lobão-Tello ◽  
Enrique Paredes Herbach ◽  
María José Navarrete-Talloni
Keyword(s):  
Lymph Nodes ◽  
Cervus Elaphus ◽  
Red Deer ◽  
Fallow Deer ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Intestinal Lesions ◽  
Individual Variations ◽  
Gross Lesions ◽  
Mycobacterium Avium Subsp Paratuberculosis

ABSTRACT: Paratuberculosis is a disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) that affects domestic and wild ruminants. The most common gross lesions are emaciation and corrugation and thickening of the mucosa of the small intestine. Mesenteric lymph nodes might be enlarged. For the present study, 14 red deer and 9 fallow deer from game reserves or venison farms were analyzed. The lesions found correspond to those found by other authors in other geographic locations, except for some differences in histopathological examinations. Among these differences, stands out that intestinal lesions were concentrated mostly in the ileum and granulomas were shown to be more frequent in this section of the intestine than in the corresponding lymph node. Furthermore, in multibacillary lesions the inflammatory infiltrate in the lymph nodes was mainly composed of macrophages. These differences may be due to individual variations of the animals, the stage of disease or a different strain of the pathogen. This study allowed to obtain basic information about the disease and to describe patterns of lesions found in red deer and fallow deer with prediagnosis of clinical paratuberculosis which were not described in the literature before.

scholarly journals Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy

2012 ◽  
Vol 49 (No. 7) ◽  
pp. 225-236 ◽  
Author(s):  
T. Amemori ◽  
L. Matlova ◽  
O. A Fischer ◽  
W. Y Ayele ◽  
M. Machackova ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Laparoscopic Approach ◽  
Ileocecal Valve ◽  
Mycobacterium Avium Subsp ◽  
Sample Collection ◽  
Mesenteric Lymph Nodes ◽  
Laparoscopic Biopsy ◽  
Mycobacterium Avium Subsp Paratuberculosis

The gastrointestinal tract (GIT) is a major target for Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) in cattle. Culture examination was achieved in tissue samples obtained from 10 different regions of the GIT (proximal and distal parts of the duodenum, proximal, middle and distal parts of the jejunum, proximal and distal parts of the ileum, the ileocecal valve, the caecum and the rectum) and their adjacent lymph nodes. The culture results were statistically analysed to elucidate the distribution of M. a. paratuberculosis in the GIT. A total of 63 cows older than 24 months were diagnosed with paratuberculosis by faecal and tissue cultures. The better detection rate of M. a. paratuberculosis was found in the mucosae from the jejunum to the ileocecal valve and in the lymph nodes from the jejunum to the caecum. The mean number of colony forming units (CFU) in the mucosae and the lymph nodes of the distal jejunum and the proximal ileum was significantly higher than that in the mucosae of the duodenum, the caecum and the rectum, and in the lymph nodes of the duodenum and the rectum, respectively (p < 0.05). Laparoscopic biopsy attempted out on 4 animals to test its potential use for sample collection from the statistically optimal mesenteric lymph nodes; but resulted in an abortive attempt because these targets were encircled by the intestines, the pressure of which complicated the laparoscopic approach.

scholarly journals A mixed infection of Mycobacterium avium subsp. paratuberculosis and M. a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report

2008 ◽  
Vol 53 (No. 8) ◽  
pp. 445-451 ◽  
Author(s):  
M. Moravkova ◽  
I. Trcka ◽  
J. Lamka ◽  
I. Pavlik
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Cervus Elaphus ◽  
Mixed Infection ◽  
Red Deer ◽  
Chronic Diarrhoea ◽  
Mesenteric Lymph Nodes ◽  
Tissue Samples ◽  
Mesenteric Lymph ◽  
Rflp Type

A mixed infection with <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (<i>MAP</i>) and <i>Mycobacterium avium</i> subsp. <i>hominissuis</i> (<i>MAH</i>) in one naturally infected red deer stag from a game park is described. The animal was euthanized because of symptoms of poor condition, weight loss and chronic diarrhoea. In spite of that, pathological lesions were observed only in the mesenteric lymph nodes, which were five to ten times enlarged with confluent caseous granulomas of 1 to 10 mm in size. Mycobacteria were isolated from all studied samples: a mixed infection of <i>MAP</i> and <i>MAH</i> was confirmed by multiplex PCR for the detection of IS <i>900</i>, IS<i>901</i>1, IS<i>1245</i> and <i>dnaJ</i>. MAP</i> of the identical IS<i>900</i> <i>BstE</i>II RFLP type C1 was isolated from all tissue samples and faeces. <i>MAH</i> isolates were detected in six examined tissue samples, including three mesenteric lymph nodes with caseous granulomas. Only minor differences in the band numbers and position of four different IS<i>1245</i> <i>Pvu</i>II RFLP patterns of <i>MAH</i> isolates were found. It follows from these results that red deer may potentially be infected with <i>MAH</i>, when a <i>MAP</i> infection is under way.

Pathology of the Mononuclear Cell Leukemia of Fischer Rats. I. Morphologic Studies

1983 ◽  
Vol 20 (6) ◽  
pp. 698-708 ◽  
Author(s):  
P. C. Stromberg ◽  
L. M. Vogtsberger
Keyword(s):  
Lymph Nodes ◽  
Mononuclear Cell ◽  
Significant Risk ◽  
Extramedullary Hematopoiesis ◽  
Cell Leukemia ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Lymphoid Depletion ◽  
Gross Lesions ◽  
Red Pulp

Pathological evaluations were done on 205 rats with mononuclear cell leukemia. Leukemia was diagnosed in 22.2% of males and 20.4% of females with significant risk beginning at 20 months of age. Mononuclear cell leukemia was responsible for 50% of early deaths in two-year studies. Clinically, rats became depressed, pale, icteric and had palpably enlarged spleens. Gross lesions included splenomegaly, enlarged mesenteric lymph nodes, and mottled livers. Hemorrhages occurred in the lungs, brain, and lymph nodes. Histological examination demonstrated that spleen and liver were most consistently and seriously involved, although numerous other organs contained leukemic infiltrates of variable severity. Spleens exhibited diffuse leukemic infiltration of the red pulp, follicular lymphoid depletion, and decrease in both extramedullary hematopoiesis and hemosiderin. Liver lesions consisted of diffuse centrilobular degeneration and necrosis. Erythrophagocytosis by tumor cells was common in the spleen and observed in liver, lymph nodes, and adrenals. The disease appeared to originate in the spleen. Bone marrow infiltration occurred late relative to spleen involvement and was present in less than half of the rats.

scholarly journals Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report

2011 ◽  
Vol 56 (No. 6) ◽  
pp. 294-301 ◽  
Author(s):  
M. Blahutkova ◽  
P. Fictum ◽  
M. Skoric ◽  
B. Bezdekova ◽  
P. Jahn ◽  
...  
Keyword(s):  
Real Time ◽  
Mycobacterium Avium ◽  
Real Time Pcr ◽  
Intestinal Content ◽  
Mycobacterium Avium Subsp ◽  
Pathological Findings ◽  
Quantitative Real Time Pcr ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Qpcr Method

This report describes new possibilities for intravital and post mortem diagnosis of avian mycobacteriosis in horses using the quantitative real time PCR (qPCR) method. Using this method, Mycobacterium avium subsp. hominissuis was diagnosed in two sibling Fjord horses. In the first horse, M. a. hominissuis was detected by qPCR in numbers of 2.89 &times; 10<sup>5</sup> and 1.47 &times; 10<sup>4</sup> cells per 1 g of intestinal content and mesenteric lymph nodes, respectively; in the second horse, faeces and mesenteric lymph node samples showed numbers of 6.31 &times; 10<sup>5</sup> and 3.36 &times; 10<sup>6</sup> cells per 1 g of tissue, respectively. Another aim of this study was to comprehensively describe clinical and pathological findings in both animals.

scholarly journals Pathology of Inhalational Anthrax Infection in the African Green Monkey

2007 ◽  
Vol 44 (5) ◽  
pp. 716-721 ◽  
Author(s):  
N. A. Twenhafel ◽  
E. Leffel ◽  
M. L. M. Pitt
Keyword(s):  
Lymph Nodes ◽  
Rhesus Macaques ◽  
Current Model ◽  
High Dose ◽  
Mesenteric Lymph Nodes ◽  
Primate Model ◽  
Mesenteric Lymph ◽  
Anthrax Infection ◽  
Gross Lesions ◽  
Ames Strain

There is a critical need for an alternative nonhuman primate model for inhalational anthrax infection because of the increasingly limited supply and cost of the current model. This report describes the pathology in 12 African green monkeys (AGMs) that succumbed to inhalational anthrax after exposure to a low dose (presented dose 200–2 X 104 colony-forming units [cfu]) or a high dose (presented dose 2 X 104–1 X 107 cfu) of Bacillus anthracis (Ames strain) spores. Frequent gross lesions noted in the AGM were hemorrhage and edema in the lung, mediastinum, and mediastinal lymph nodes; pleural and pericardial effusions; meningitis; and gastrointestinal congestion and hemorrhage. Histopathologic findings included necrohemorrhagic lymphadenitis of mediastinal, axillary, inguinal, and mesenteric lymph nodes; mediastinal edema; necrotizing splenitis; meningitis; and congestion, hemorrhage, and edema of the lung, mesentery, mesenteric lymph nodes, gastrointestinal tract, and gonads. Pathologic changes in AGMs were remarkably similar to what has been reported in rhesus macaques and humans that succumbed to inhalational anthrax; thus, AGMs could serve as useful models for inhalation anthrax studies.

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