Stimulation of ex vitro growth of Rhododendron hybrids ‘Nova Zembla’ and ‘Alfred’ by inoculation of roots with Serendipita indica

Experiments were carried out to study the consequences of inoculating Rhododendron ‘Nova Zembla’ and ‘Alfred’ microcuttings and young plants with Serendipita indica (formerly Piriformospora indica), an endophytic fungus. The inoculation at the in vitro rooting stage decreased the in vitro root and shoot quality, but after planting microplants in a greenhouse in a non-fertilised peat substrate, the plants grew 33% taller, with 10% more leaves and with more abundant roots in comparison with the non-inoculated microplants. The young plants rooted in vitro, acclimated in a greenhouse in a peat substrate and inoculated with S. indica at the time of the transplantation to the pots displayed, after 15 months, an increase in the shoot lengths by 35% and 13%, depending on the cultivar, in the leaf number by 47% and in the chlorophyll content by 31% when compared with the non-inoculated plants. The beneficial effect of S. indica on the young Rhododendron plants indicates the possibility of its practical use in the nursery production of this plant.

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Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

Glasnik Sumarskog fakulteta ◽

10.2298/gsf1511099m ◽

2015 ◽

pp. 99-112

Author(s):

Marija Markovic ◽

Dragana Skocajic ◽

Mihailo Grbic ◽

Matilda Djukic ◽

Dragica Obratov-Petkovic ◽

...

Keyword(s):

Medicinal Plant ◽

Nutrient Solution ◽

Ph Value ◽

Ex Vitro Rooting ◽

In Vitro Rooting ◽

Efficient Production ◽

Ms Medium ◽

Ex Vitro ◽

Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

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589 PS 132 TISSUE PROLIFERATION IN ELEPIDOTE RHODODENDRONS

HortScience ◽

10.21273/hortsci.29.5.516c ◽

1994 ◽

Vol 29 (5) ◽

pp. 516c-516 ◽

Cited By ~ 2

Author(s):

Richard K. Kiyomoto ◽

Mark H. Brand

Keyword(s):

Shoot Proliferation ◽

Shoot Tips ◽

Differential Mortality ◽

Free Medium ◽

Ex Vitro ◽

Shoot Morphology ◽

Normal Shoot ◽

Tissue Proliferation ◽

Ex Vitro Growth

Experiments were conducted on tissue proliferation (TP) development and in vitro and ex vitro growth of tissues from plants with (TP+) and without TP (TP-). In 1993 the increase in TP in one-, two-, and three-yr-old `Holden' and `Besse Howells' was 3%, 52%. and 32% and 10%, 26% and 21%, respectively. No differential mortality was observed. Shoot tip cultures initated from TP+ and TP- `Montego' showed 10-12 mo were required for miniaturiziation and multiplication in TP- shoot tips and 4 mo in TP+ shoot tips. TP- cultures require 10 uM 2-iP for normal shoot proliferation; whereas TP+ cultures had to be transferred to hormone-free medium after 6 mo to maintain normal shoot morphology. Cutting propagation from TP- and TP+ plants older than 5 yr, showed persistence of morphological aberrations associated with TP+ plants.

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044 VARIETAL DIFFERENCES IN EARLY EX VITRO GROWTH AND DEVELOPMENT OF MICROPROPAGATED PONTEDERIA CORDATA L.

HortScience ◽

10.21273/hortsci.29.5.434b ◽

1994 ◽

Vol 29 (5) ◽

pp. 434b-434

Author(s):

Myrna Stenberg ◽

Michael E. Kane ◽

Nancy Philman

Keyword(s):

Growth Rate ◽

Growth And Development ◽

Wetland Plants ◽

Ex Vitro ◽

Uptake Capacity ◽

Early Screening ◽

Pontederia Cordata ◽

Plantlet Growth ◽

Ex Vitro Growth

Micropropagation is a commercially viable and ecologically sound method for producing native herbaceous wetland plants used for wetland revegetation projects. The ability to rapidly screen, select and store germplasm of wetland species genotypes with desirable characteristics of growth rate and habit, nutrient uptake capacity, and/or substrate preference would significantly impact how micropropagated wetland plants are marketed. Early screening of plantlet growth ex vitro may provide an efficient method to select for specific characteristics of growth rate and habit. Five micropropagated lines of Pontederia cordata of differing phenotype were established in vitro from Florida populations. Rooted microcuttings were established ex vitro in a shallow outdoor tank. Growth and development were monitored over a 9 week period. Significant differences in shoot growth and number, leaf area and number, flowering and dry weights were observed between the different Pontederia cordata varieties.

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A COMPREHENSIVE DESCRIPTION OF EX-VITRO ROOTING OF PISTACHIO ROOTSTOCKS GROWN IN PHOTOMIXOTROPHIC AND PHOTOAUTOTROPHIC CONDITION

FRUIT GROWING RESEARCH ◽

10.33045/fgr.v36.2020.08 ◽

2020 ◽

Vol 36 ◽

pp. 66-74

Author(s):

Mohammad Javad Mahmoudi Meimand ◽

Mohammad Hossein Shamshiri ◽

Khalil Malekzadeh ◽

Mohammad Reza Dehghani

Keyword(s):

Sucrose Concentration ◽

Ex Vitro Rooting ◽

In Vitro Rooting ◽

Naphthalene Acetic Acid ◽

Ex Vitro ◽

Indole Butyric Acid ◽

Micro Propagation ◽

The Media ◽

Photoautotrophic Condition

As a basic principle, ex-vitro rhizogenesis increases the micropropagation efﬁciency of Micro propagation in any plant from both biological and economic viewpoints. In the current study, we surveyed the effects of number of air exchanges along with sucrose concentration on in-vitro rooting of two pistachio rootstocks consisting of UCB1 and Qazvini versus ex-vitro rooting. Based on our findings for the UCB1 rootstock, microshoot ex-vitro rooting reached the highest percentage (63.70 %) after six weeks' treatment with indole butyric acid (IBA) (5000 ppm) and free naphthalene acetic acid (NAA), while for Qazvini rootstock treated with NAA (6000 ppm) along with IBA (5000 ppm), rooting achieved 35.06%. Photomixotrophic resulted from decreasing sucrose concentration from 30 to 15 (g L-1) in corporation with ventilation condition increased UCB1 rooting (67.89%) as well as plant survival (58.34%). For Qazvini rootstock, maximum sucrose concentration (30 g L-1) improved rooting parameters. For in-vitro rooting experiment, rooting percentage of UCB1 plantlets as well as the main and lateral produced roots were higher in media supplemented with (1 mg l-1) IBA, free NAA, and BA. Regarding Qazvini rootstock, the highest in-vitro rooting percentage (43.75%) and root length were associated with the media supplemented with BA (0.5 mg l-1), IBA (2 mg l-1), and NAA (2 mg l-1). As a result, for both the studied rootstocks, better rooting parameters were observed in the ex-vitro rooted microshoots than in-vitro rooted.

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