scholarly journals 044 VARIETAL DIFFERENCES IN EARLY EX VITRO GROWTH AND DEVELOPMENT OF MICROPROPAGATED PONTEDERIA CORDATA L.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 434b-434
Author(s):  
Myrna Stenberg ◽  
Michael E. Kane ◽  
Nancy Philman
Keyword(s):  
Growth Rate ◽  
Growth And Development ◽  
Wetland Plants ◽  
Ex Vitro ◽  
Uptake Capacity ◽  
Early Screening ◽  
Pontederia Cordata ◽  
Plantlet Growth ◽  
Ex Vitro Growth

Micropropagation is a commercially viable and ecologically sound method for producing native herbaceous wetland plants used for wetland revegetation projects. The ability to rapidly screen, select and store germplasm of wetland species genotypes with desirable characteristics of growth rate and habit, nutrient uptake capacity, and/or substrate preference would significantly impact how micropropagated wetland plants are marketed. Early screening of plantlet growth ex vitro may provide an efficient method to select for specific characteristics of growth rate and habit. Five micropropagated lines of Pontederia cordata of differing phenotype were established in vitro from Florida populations. Rooted microcuttings were established ex vitro in a shallow outdoor tank. Growth and development were monitored over a 9 week period. Significant differences in shoot growth and number, leaf area and number, flowering and dry weights were observed between the different Pontederia cordata varieties.

scholarly journals In Vitro And Ex Vitro Propagation Of A Wild-Extinct Fern Lygodium Circinnatum (Burn.F) Sw. Grown In Bali

2016 ◽  
Vol 16 (2) ◽  
pp. 131
Author(s):  
Rindang Dwiyani
Keyword(s):  
Wild Species ◽  
Growth And Development ◽  
Ex Vitro ◽  
Foliar Fertilizer ◽  
Full Strength

Research concerning of propagation of a wild-extinct fern Lygodium circinnatum had been done at Faculty of Agriculture, Udayana University, Denpasar Bali Indonesia. At some places in Indonesia, as well as Bali, this species is used as materials for making handicraft. In Bali, the species grows wildly in the forest and it is almost extinct due to over gathering. This study aimed to find out method for domestication of L. circinnatum, therefore this wild species can be cultivated, provided materials for making handicraft and might solve the problem of extinction. Various media for growing spores of L. circinnatum in vitro and ex vitro were trialed. In conclusion, full strength of MS media without sugar was the most appropriate media for growth and development of spores of L. circinnatum in vitro. While for ex vitro, the appropriate media were paddy silt-soil and decomposed leaf either with or without addition of foliar fertilizer. However, we suggested ex-vitro cultivation was more appropriate, the technique was much easier and the spores grew faster compared to those of in-vitro.

In vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium

1993 ◽  
Vol 73 (4) ◽  
pp. 1105-1113 ◽  
Author(s):  
Ribo Deng ◽  
Danielle J. Donnelly
Keyword(s):  
Relative Humidity ◽  
Co2 Enrichment ◽  
Dry Weight ◽  
Early Growth ◽  
Rubus Idaeus ◽  
Ex Vitro ◽  
Red Raspberry ◽  
Plantlet Growth ◽  
Murashige Skoog

Micropropagated shoots of red raspberry (Rubus idaeus L. ’Comet’) were rooted on modified Murashige-Skoog medium lacking sucrose, in specially constructed plexiglass chambers, under ambient (340 ± 20 ppm) or enriched (1500 ± 50 ppm) CO2 and ambient (ca. 100%) or reduced (90 ± 5%) relative humidity. Cultured plantlets were evaluated for their survival, rooting and relative vigor, leaf and root number, stem and root length, total leaf area, total fresh and dry weight, gas exchange rate, and stomatal features, prior to transplantation to soil and at intervals for 6 wk ex vitro. In vitro CO2 enrichment promoted plantlet growth, rooting and both the survival and early growth of transplants. CO2 enrichment increased stomatal aperture of plantlet leaves but did not apparently increase water stress at transplantation. Reduced in vitro RH did not affect plantlet growth but decreased stomatal apertures and stomatal index on leaves of cultured plantlets and promoted both the survival and early growth of transplants. In vitro CO2 and RH levels did not affect the photosynthetic rate of either plantlets or transplants. Only the stomata on leaves of plantlets from the ambient CO2 and reduced RH treatment were functional. Normal stomatal function was not observed in persistent leaves of transplants from the other treatments, even 2 wk after transplantation. In vitro CO2 enrichment acted synergistically with RH reduction in improving growth of plantlets both in vitro and ex vitro. Hardened red raspberry plantlets obtained through CO2 enrichment and RH reduction survived direct transfer to ambient greenhouse conditions without the necessity for specialized ex vitro acclimatization treatment. Key words: Acclimatization, growth analysis, photosynthesis, Rubus idaeus L., stomata, tissue culture

scholarly journals 589 PS 132 TISSUE PROLIFERATION IN ELEPIDOTE RHODODENDRONS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 516c-516 ◽  
Author(s):  
Richard K. Kiyomoto ◽  
Mark H. Brand
Keyword(s):  
Shoot Proliferation ◽  
Shoot Tips ◽  
Differential Mortality ◽  
Free Medium ◽  
Ex Vitro ◽  
Shoot Morphology ◽  
Normal Shoot ◽  
Tissue Proliferation ◽  
Ex Vitro Growth

Experiments were conducted on tissue proliferation (TP) development and in vitro and ex vitro growth of tissues from plants with (TP+) and without TP (TP-). In 1993 the increase in TP in one-, two-, and three-yr-old `Holden' and `Besse Howells' was 3%, 52%. and 32% and 10%, 26% and 21%, respectively. No differential mortality was observed. Shoot tip cultures initated from TP+ and TP- `Montego' showed 10-12 mo were required for miniaturiziation and multiplication in TP- shoot tips and 4 mo in TP+ shoot tips. TP- cultures require 10 uM 2-iP for normal shoot proliferation; whereas TP+ cultures had to be transferred to hormone-free medium after 6 mo to maintain normal shoot morphology. Cutting propagation from TP- and TP+ plants older than 5 yr, showed persistence of morphological aberrations associated with TP+ plants.

scholarly journals Morphological Development of Lingonberry as Affected by In Vitro and Ex Vitro Propagation Methods and Source Propagule

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 760-763 ◽  
Author(s):  
Samir C. Debnath
Keyword(s):  
Vegetative Growth ◽  
Nutrient Medium ◽  
Growth And Development ◽  
Shoot Proliferation ◽  
Morphological Development ◽  
Stem Cuttings ◽  
Ex Vitro ◽  
Propagation Methods ◽  
In Vitro Shoot Proliferation

The growth and development of lingonberry (Vaccinium vitis-idaea L.) plants propagated either by conventional softwood cuttings or by in vitro shoot proliferation from nodal explants and by shoot regeneration from excised leaves of micropropagated shoots, were studied in cultivars `Regal', `Splendor', and `Erntedank'. Significant differences were observed between the treatments. After 3 years of growth, the in vitro-derived plants produced more stems, leaves, and rhizomes than the conventional cuttings which rarely produced rhizomes. In vitro culture on nutrient medium apparently induces the juvenile branching characteristics that favor rhizome production. This increase in vegetative growth and rhizome yield of in vitro-derived plants over stem cuttings varied among genotypes.

scholarly journals Influence of propagation method a nd indole-3-butyric acid on growth and development of in vitro- and ex vitro-derived lingonberry plants

2006 ◽  
Vol 86 (1) ◽  
pp. 235-243 ◽  
Author(s):  
Samir C Debnath
Keyword(s):  
Butyric Acid ◽  
Growth And Development ◽  
Shoot Growth ◽  
Adventitious Shoot ◽  
Ex Vitro ◽  
Adventitious Shoot Regeneration ◽  
Propagation Methods ◽  
Rhizome Morphology ◽  
Rhizome Development

The effects of two propagation methods and four indole-3-butyric acid (IBA) concentrations were studied in two cultivars of lingonberry (Vaccinium vitis-idaea L.). Cultivars Regal and Erntedank, propagated by conventional softwood cuttings (SC) and adventitious shoot regeneration from excised leaves of micropropagated shoots (LC), were evaluated for growth and morphology. Significant two- and three-way interactions for shoot and rhizome characteristics were observed among the treatments. The LC plants produced shorter and less-vigorous shoots but had more stems, branches, leaves and rhizomes in contrast to conventional cuttings, which rarely produced rhizomes. IBA had an effect on shoot and rhizome morphology, but not on the frequency of rhizome formation. In vitro culture on zeatin-containing nutrient medium apparently induces the juvenile branching characteristics that favored enhanced rhizome production. The advantage of shoot and rhizome production of adventitiously produced LC plants over SC plants varied between the genotypes. Erntedank plants had better shoot growth and rhizome development across propagation methods than did Regal plants. Key words: Vaccinium vitis-idaea, propagation, regeneration, cuttings, indole-3-butyric acid

Effects of supporting materials in in vitro acclimatization stage on ex vitro growth of wasabi plants

2020 ◽  
Vol 261 ◽  
pp. 109042 ◽  
Author(s):  
Nhung Ngoc Hoang ◽  
Yoshiaki Kitaya ◽  
Toshio Shibuya ◽  
Ryosuke Endo
Keyword(s):  
Ex Vitro ◽  
Ex Vitro Growth ◽  
Supporting Materials

scholarly journals Height and number of shoots on the survival and development of micropropagated bamboo plantlets during pre-acclimatization

2019 ◽  
Vol 49 ◽  
Author(s):  
Paulo Arthur Almeida do Vale ◽  
João Bosco de Oliveira Júnior ◽  
Frederico Henrique da Silva Costa ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Survival Rates ◽  
Biomass Accumulation ◽  
Dry Mass ◽  
Ex Vitro ◽  
In Vitro Multiplication ◽  
Dry Biomass ◽  
Survival And Development ◽  
Ex Vitro Growth ◽  
The Relationship

ABSTRACT During the in vitro multiplication of bamboo plantlets, it is common the formation of shoots aggregates. Once individualized, these can yield a greater number of plantlets than if planted in clusters. This study aimed to evaluate the effect of the relationship between height and number of initial shoots in micropropagated bamboo plantlets on the survival and development of plants, during the pre-acclimatization stage. Guadua aff. chaparensis shoots, after successive subcultures of in vitro multiplication, were classified into three height classes (2.5-5.0 cm; 5.1-10.0 cm; 10.1-15.0 cm) and number of aggregate shoots (one shoot per plantlet/single-stem plantlet, two and three shoots per plantlet) and pre-acclimatized in a commercial substrate composition plus washed sand. The plantlets were evaluated for survival, height, number of new shoots and roots, shoot and root fresh and dry mass. In plantlets from micropropagation, the plant height does not influence the survival rates, being acclimatized preferably at heights between 5.0 cm and 15.0 cm, with survival rates of up to 97 %. Plantlets with height starting at 5.1 cm and composed of 2 or 3 initial shoots show a greater vigor and ex vitro growth, a fact evidenced by the higher values obtained in relation to height and emission of new shoots and roots, as well as a greater fresh and dry biomass accumulation.

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