ASYMBIOTIC CULTIVATION IN VITRO OF THE ENDANGERED ORCHID CYPRIPEDIUM CALCEOLUS L. AND SOME ASPECTS OF EX VITRO GROWTH

2009 ◽  
pp. 539-544
Author(s):  
D. Klavina ◽  
I. Druva-Lusite ◽  
A. Gailite
Keyword(s):  
Ex Vitro ◽  
Endangered Orchid ◽  
Ex Vitro Growth ◽  
Cypripedium Calceolus ◽  
Cultivation In Vitro

scholarly journals 589 PS 132 TISSUE PROLIFERATION IN ELEPIDOTE RHODODENDRONS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 516c-516 ◽  
Author(s):  
Richard K. Kiyomoto ◽  
Mark H. Brand
Keyword(s):  
Shoot Proliferation ◽  
Shoot Tips ◽  
Differential Mortality ◽  
Free Medium ◽  
Ex Vitro ◽  
Shoot Morphology ◽  
Normal Shoot ◽  
Tissue Proliferation ◽  
Ex Vitro Growth

Experiments were conducted on tissue proliferation (TP) development and in vitro and ex vitro growth of tissues from plants with (TP+) and without TP (TP-). In 1993 the increase in TP in one-, two-, and three-yr-old `Holden' and `Besse Howells' was 3%, 52%. and 32% and 10%, 26% and 21%, respectively. No differential mortality was observed. Shoot tip cultures initated from TP+ and TP- `Montego' showed 10-12 mo were required for miniaturiziation and multiplication in TP- shoot tips and 4 mo in TP+ shoot tips. TP- cultures require 10 uM 2-iP for normal shoot proliferation; whereas TP+ cultures had to be transferred to hormone-free medium after 6 mo to maintain normal shoot morphology. Cutting propagation from TP- and TP+ plants older than 5 yr, showed persistence of morphological aberrations associated with TP+ plants.

scholarly journals 044 VARIETAL DIFFERENCES IN EARLY EX VITRO GROWTH AND DEVELOPMENT OF MICROPROPAGATED PONTEDERIA CORDATA L.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 434b-434
Author(s):  
Myrna Stenberg ◽  
Michael E. Kane ◽  
Nancy Philman
Keyword(s):  
Growth Rate ◽  
Growth And Development ◽  
Wetland Plants ◽  
Ex Vitro ◽  
Uptake Capacity ◽  
Early Screening ◽  
Pontederia Cordata ◽  
Plantlet Growth ◽  
Ex Vitro Growth

Micropropagation is a commercially viable and ecologically sound method for producing native herbaceous wetland plants used for wetland revegetation projects. The ability to rapidly screen, select and store germplasm of wetland species genotypes with desirable characteristics of growth rate and habit, nutrient uptake capacity, and/or substrate preference would significantly impact how micropropagated wetland plants are marketed. Early screening of plantlet growth ex vitro may provide an efficient method to select for specific characteristics of growth rate and habit. Five micropropagated lines of Pontederia cordata of differing phenotype were established in vitro from Florida populations. Rooted microcuttings were established ex vitro in a shallow outdoor tank. Growth and development were monitored over a 9 week period. Significant differences in shoot growth and number, leaf area and number, flowering and dry weights were observed between the different Pontederia cordata varieties.

Effects of supporting materials in in vitro acclimatization stage on ex vitro growth of wasabi plants

2020 ◽  
Vol 261 ◽  
pp. 109042 ◽  
Author(s):  
Nhung Ngoc Hoang ◽  
Yoshiaki Kitaya ◽  
Toshio Shibuya ◽  
Ryosuke Endo
Keyword(s):  
Ex Vitro ◽  
Ex Vitro Growth ◽  
Supporting Materials

scholarly journals Height and number of shoots on the survival and development of micropropagated bamboo plantlets during pre-acclimatization

2019 ◽  
Vol 49 ◽  
Author(s):  
Paulo Arthur Almeida do Vale ◽  
João Bosco de Oliveira Júnior ◽  
Frederico Henrique da Silva Costa ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Survival Rates ◽  
Biomass Accumulation ◽  
Dry Mass ◽  
Ex Vitro ◽  
In Vitro Multiplication ◽  
Dry Biomass ◽  
Survival And Development ◽  
Ex Vitro Growth ◽  
The Relationship

ABSTRACT During the in vitro multiplication of bamboo plantlets, it is common the formation of shoots aggregates. Once individualized, these can yield a greater number of plantlets than if planted in clusters. This study aimed to evaluate the effect of the relationship between height and number of initial shoots in micropropagated bamboo plantlets on the survival and development of plants, during the pre-acclimatization stage. Guadua aff. chaparensis shoots, after successive subcultures of in vitro multiplication, were classified into three height classes (2.5-5.0 cm; 5.1-10.0 cm; 10.1-15.0 cm) and number of aggregate shoots (one shoot per plantlet/single-stem plantlet, two and three shoots per plantlet) and pre-acclimatized in a commercial substrate composition plus washed sand. The plantlets were evaluated for survival, height, number of new shoots and roots, shoot and root fresh and dry mass. In plantlets from micropropagation, the plant height does not influence the survival rates, being acclimatized preferably at heights between 5.0 cm and 15.0 cm, with survival rates of up to 97 %. Plantlets with height starting at 5.1 cm and composed of 2 or 3 initial shoots show a greater vigor and ex vitro growth, a fact evidenced by the higher values obtained in relation to height and emission of new shoots and roots, as well as a greater fresh and dry biomass accumulation.

scholarly journals Cultivation of Cypripedium calceolus L. ex vitro seedlings in outdoor conditions: Short communication

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 162-170
Author(s):  
J. Obdržálek
Keyword(s):  
Average Length ◽  
Additional Treatment ◽  
Ex Vitro ◽  
Private Laboratory ◽  
Cypripedium Calceolus ◽  
Pumice Sand ◽  
Bare Root ◽  
Lime Powder

The paper presents first positive results of the experiment with three-year outdoor cultivation of Cypripedium calceolus L. ex vitro seedlings in the Czech Republic. They were propagated in vitro from mature seeds of Carpathian provenance in a Prague private laboratory. In April 2006 after three months cool refrigerating at 4°C the seedlings were prepared for planting. The rhizomes with 4 to 12 roots and visible dormant buds were used. They were planted in two types of substrates: mixture AN on the basis of liadrain (burned clay pebbles) and mixture BN on the basis of granodiorite. Both mixtures were amended with perlite, pumice, sand, zeolite and dolomite lime powder. The mineral substrates proved to be stable and convenient for transfer and cultivation of ex vitro seedlings. Additional treatment with lignohumate in other two variants of the experiment did not improve the effect. The seedlings were grown outdoors on a shaded bed till the retracting leaves. They overwintered in a cold glasshouse with temperature close to zero from late November to March. The substrates did not visually influence the phase of sprouting, the phase of growth and retracting of the plants. At the end of the third growing season the yield of 4-year-old seedlings with two to four leaves ranged from 83% to 98% in four variants. In November 2008 seedlings were taken up from the mixes and were evaluated as bare root plants. The number of the living plants with visible new buds and the quality of root system were recorded and evaluated. The average length of roots in mixture A on the basis of liadrain and B on the basis of granodiorite was 14.5 cm and 12.1 cm, respectively. The rhizomes were planted into new mixtures immediately. These seedlings will be able to grow up to the blooming size during two or three seasons. Seven year-old potted seedlings of C. calceolus will be planted into gene resource area of the Silva Tarouca Research Institute for Landscape and Ornamental Gardening at Průhonice.

scholarly journals Cryopreservation and Acclimatization of Lycopersicon esculentum (Mill.) Genotypes

2014 ◽  
Vol 42 (2) ◽  
pp. 466-471 ◽  
Author(s):  
Ana COSTE ◽  
Sergiu VALIMAREANU ◽  
Adela HALMAGYI
Keyword(s):  
Lycopersicon Esculentum ◽  
Liquid Nitrogen ◽  
Growth Conditions ◽  
Alginate Beads ◽  
Shoot Tips ◽  
Ex Vitro ◽  
Fresh Weight Basis ◽  
Ex Vitro Growth ◽  
Laminar Air Flow

Romanian tomato (Lycopersicon esculentum Mill.) cultivars have been cryopreserved by encapsulation-dehydration and successfully acclimatized to ex vitro growth conditions. Shoot tips were excised from in vitro grown plants then precultured for 24 h in various sucrose concentrations, dehydrated up to 6 h in laminar air flow prior to direct immersion in liquid nitrogen   (−196°C) for 24 h. Different parameters have been studied: the effects of osmoprotection and desiccation duration on the regrowth of cryopreserved shoot tips, the effects of various IBA concentrations on rooting and the ex vitro cclimatization of plants recovered from liquid nitrogen. The highest frequency of regrowth (72% cv. ‘Pontica’) was obtained when encapsulated explants were precultured in 0.5 M sucrose and the moisture content (fresh weight basis) of alginate beads was 23%. The highest rooting rates (58% to 77%) for all cultivars were observed for shoots grown on MS medium supplemented with 1.0 mg/l IBA. The rooted plants could be easily acclimatized ex vitro with up to 100% survival.

scholarly journals Modern technologies of increasing the tolerance of in vitro cultured plants to ex vitro conditions

2020 ◽  
Vol 26 ◽  
pp. 183-189
Author(s):  
L. R. Hrytsak ◽  
N. M. Drobyk
Keyword(s):  
Ex Vitro ◽  
Adaptive Potential ◽  
In Vitro Plants ◽  
Plant Cultivation ◽  
Planting Material ◽  
Chemical Conditions ◽  
Cultivation In Vitro ◽  
Physical And Chemical ◽  
Modern Technologies

Aim. To analyze the experience of Ukrainian and foreign scientists on technologies to increase the adaptive potential of cultivated in vitro plants to ex vitro conditions. Results. Modern acclimatization technologies are mainly aimed at improving the methods of adaptation of planting material of in vitro collections to ex vitro conditions. Much less attention is paid to technologies to increase plant resilience at the stage of their multiplication and growth in vitro. Integration and systematization of research results of a large number of scientists is allowed to describe the main strategies and methodological techniques, which implementation can significantly increase the adaptive potential of in vitro plants. Conclusions. Optimization of physical and chemical conditions of plant cultivation in vitro can induce changes in their phenotype, intensity of photosynthetic reactions, water balance, which increases the adaptive potential of plants and facilitates the process of their acclimatization to ex vitro conditions. Key words: in vitro plants, acclimatization to ex vitro conditions, adaptive potential, technology.

scholarly journals Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid

2017 ◽  
Vol 11 (04) ◽  
pp. 485-490 ◽  
Author(s):  
Renato Fernandes Galdiano Junior ◽  
◽  
Wagner Aparecido Vendrame ◽  
Cristiane Moretto ◽  
Ricardo Tadeu de Faria ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Ex Vitro ◽  
Ex Vitro Growth ◽  
Seed Cryopreservation

scholarly journals Effect of Chitooligosaccharide on In vitro and Ex vitro Growth of Piper nigrum L.

2019 ◽  
pp. 1-8
Author(s):  
Mai Quoc Quan ◽  
Nguyen Thi Dao ◽  
Nguyen Quang Vinh
Keyword(s):  
Sodium Hypochlorite ◽  
Culture Media ◽  
Suitable Condition ◽  
Piper Nigrum ◽  
Ex Vitro ◽  
Benzyl Adenine ◽  
Ex Vitro Growth ◽  
Molecular Signals

Oligosaccharins: oligogalacturonic, and chitooligosaccharides are known as molecular signals to induce and regulate various genes in plants. This study was conducted to deternine the effects of chitooligosaccharide on bud formula and growth of Piper nigrum in both in vitro and ex vitro. The results showed that sterilize Piper nigrum shoots with 30% sodium hypochlorite at 10 min was the most suitable condition; appropriate culture media for bud formulation was Murashige and Skoog (MS) media supplemented 30 g/L saccharose, 7,5 g/L agar, 3 mg/L N6 – benzyl adenine (BA), culture media for growth of plantlet shoot was MS media supplemented 30 g/L sacharose, 7,5 g/L agar, 1 g/L NAA, 2 mg/L IBA and 45 ppm chitooligosaccharide. Supplementation of chitooligosaccharide at concentration of 45 ppm was optimal for the growth of Piper nigrum plantlets both in vitro and ex vitro. Present study indicated that chitooligosaccharide strongly promote the growth of Piper nigrum and recommend concentration for both in vitro and ex vitro is 45 ppm.

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