Effects of supporting materials in in vitro acclimatization stage on ex vitro growth of wasabi plants

2020 ◽  
Vol 261 ◽  
pp. 109042 ◽  
Author(s):  
Nhung Ngoc Hoang ◽  
Yoshiaki Kitaya ◽  
Toshio Shibuya ◽  
Ryosuke Endo
Keyword(s):  
Ex Vitro ◽  
Ex Vitro Growth ◽  
Supporting Materials

scholarly journals 589 PS 132 TISSUE PROLIFERATION IN ELEPIDOTE RHODODENDRONS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 516c-516 ◽  
Author(s):  
Richard K. Kiyomoto ◽  
Mark H. Brand
Keyword(s):  
Shoot Proliferation ◽  
Shoot Tips ◽  
Differential Mortality ◽  
Free Medium ◽  
Ex Vitro ◽  
Shoot Morphology ◽  
Normal Shoot ◽  
Tissue Proliferation ◽  
Ex Vitro Growth

Experiments were conducted on tissue proliferation (TP) development and in vitro and ex vitro growth of tissues from plants with (TP+) and without TP (TP-). In 1993 the increase in TP in one-, two-, and three-yr-old `Holden' and `Besse Howells' was 3%, 52%. and 32% and 10%, 26% and 21%, respectively. No differential mortality was observed. Shoot tip cultures initated from TP+ and TP- `Montego' showed 10-12 mo were required for miniaturiziation and multiplication in TP- shoot tips and 4 mo in TP+ shoot tips. TP- cultures require 10 uM 2-iP for normal shoot proliferation; whereas TP+ cultures had to be transferred to hormone-free medium after 6 mo to maintain normal shoot morphology. Cutting propagation from TP- and TP+ plants older than 5 yr, showed persistence of morphological aberrations associated with TP+ plants.

scholarly journals 044 VARIETAL DIFFERENCES IN EARLY EX VITRO GROWTH AND DEVELOPMENT OF MICROPROPAGATED PONTEDERIA CORDATA L.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 434b-434
Author(s):  
Myrna Stenberg ◽  
Michael E. Kane ◽  
Nancy Philman
Keyword(s):  
Growth Rate ◽  
Growth And Development ◽  
Wetland Plants ◽  
Ex Vitro ◽  
Uptake Capacity ◽  
Early Screening ◽  
Pontederia Cordata ◽  
Plantlet Growth ◽  
Ex Vitro Growth

Micropropagation is a commercially viable and ecologically sound method for producing native herbaceous wetland plants used for wetland revegetation projects. The ability to rapidly screen, select and store germplasm of wetland species genotypes with desirable characteristics of growth rate and habit, nutrient uptake capacity, and/or substrate preference would significantly impact how micropropagated wetland plants are marketed. Early screening of plantlet growth ex vitro may provide an efficient method to select for specific characteristics of growth rate and habit. Five micropropagated lines of Pontederia cordata of differing phenotype were established in vitro from Florida populations. Rooted microcuttings were established ex vitro in a shallow outdoor tank. Growth and development were monitored over a 9 week period. Significant differences in shoot growth and number, leaf area and number, flowering and dry weights were observed between the different Pontederia cordata varieties.

scholarly journals Height and number of shoots on the survival and development of micropropagated bamboo plantlets during pre-acclimatization

2019 ◽  
Vol 49 ◽  
Author(s):  
Paulo Arthur Almeida do Vale ◽  
João Bosco de Oliveira Júnior ◽  
Frederico Henrique da Silva Costa ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Survival Rates ◽  
Biomass Accumulation ◽  
Dry Mass ◽  
Ex Vitro ◽  
In Vitro Multiplication ◽  
Dry Biomass ◽  
Survival And Development ◽  
Ex Vitro Growth ◽  
The Relationship

ABSTRACT During the in vitro multiplication of bamboo plantlets, it is common the formation of shoots aggregates. Once individualized, these can yield a greater number of plantlets than if planted in clusters. This study aimed to evaluate the effect of the relationship between height and number of initial shoots in micropropagated bamboo plantlets on the survival and development of plants, during the pre-acclimatization stage. Guadua aff. chaparensis shoots, after successive subcultures of in vitro multiplication, were classified into three height classes (2.5-5.0 cm; 5.1-10.0 cm; 10.1-15.0 cm) and number of aggregate shoots (one shoot per plantlet/single-stem plantlet, two and three shoots per plantlet) and pre-acclimatized in a commercial substrate composition plus washed sand. The plantlets were evaluated for survival, height, number of new shoots and roots, shoot and root fresh and dry mass. In plantlets from micropropagation, the plant height does not influence the survival rates, being acclimatized preferably at heights between 5.0 cm and 15.0 cm, with survival rates of up to 97 %. Plantlets with height starting at 5.1 cm and composed of 2 or 3 initial shoots show a greater vigor and ex vitro growth, a fact evidenced by the higher values obtained in relation to height and emission of new shoots and roots, as well as a greater fresh and dry biomass accumulation.

scholarly journals Cryopreservation and Acclimatization of Lycopersicon esculentum (Mill.) Genotypes

2014 ◽  
Vol 42 (2) ◽  
pp. 466-471 ◽  
Author(s):  
Ana COSTE ◽  
Sergiu VALIMAREANU ◽  
Adela HALMAGYI
Keyword(s):  
Lycopersicon Esculentum ◽  
Liquid Nitrogen ◽  
Growth Conditions ◽  
Alginate Beads ◽  
Shoot Tips ◽  
Ex Vitro ◽  
Fresh Weight Basis ◽  
Ex Vitro Growth ◽  
Laminar Air Flow

Romanian tomato (Lycopersicon esculentum Mill.) cultivars have been cryopreserved by encapsulation-dehydration and successfully acclimatized to ex vitro growth conditions. Shoot tips were excised from in vitro grown plants then precultured for 24 h in various sucrose concentrations, dehydrated up to 6 h in laminar air flow prior to direct immersion in liquid nitrogen   (−196°C) for 24 h. Different parameters have been studied: the effects of osmoprotection and desiccation duration on the regrowth of cryopreserved shoot tips, the effects of various IBA concentrations on rooting and the ex vitro cclimatization of plants recovered from liquid nitrogen. The highest frequency of regrowth (72% cv. ‘Pontica’) was obtained when encapsulated explants were precultured in 0.5 M sucrose and the moisture content (fresh weight basis) of alginate beads was 23%. The highest rooting rates (58% to 77%) for all cultivars were observed for shoots grown on MS medium supplemented with 1.0 mg/l IBA. The rooted plants could be easily acclimatized ex vitro with up to 100% survival.

scholarly journals Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid

2017 ◽  
Vol 11 (04) ◽  
pp. 485-490 ◽  
Author(s):  
Renato Fernandes Galdiano Junior ◽  
◽  
Wagner Aparecido Vendrame ◽  
Cristiane Moretto ◽  
Ricardo Tadeu de Faria ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Ex Vitro ◽  
Ex Vitro Growth ◽  
Seed Cryopreservation

scholarly journals Effect of Chitooligosaccharide on In vitro and Ex vitro Growth of Piper nigrum L.

2019 ◽  
pp. 1-8
Author(s):  
Mai Quoc Quan ◽  
Nguyen Thi Dao ◽  
Nguyen Quang Vinh
Keyword(s):  
Sodium Hypochlorite ◽  
Culture Media ◽  
Suitable Condition ◽  
Piper Nigrum ◽  
Ex Vitro ◽  
Benzyl Adenine ◽  
Ex Vitro Growth ◽  
Molecular Signals

Oligosaccharins: oligogalacturonic, and chitooligosaccharides are known as molecular signals to induce and regulate various genes in plants. This study was conducted to deternine the effects of chitooligosaccharide on bud formula and growth of Piper nigrum in both in vitro and ex vitro. The results showed that sterilize Piper nigrum shoots with 30% sodium hypochlorite at 10 min was the most suitable condition; appropriate culture media for bud formulation was Murashige and Skoog (MS) media supplemented 30 g/L saccharose, 7,5 g/L agar, 3 mg/L N6 – benzyl adenine (BA), culture media for growth of plantlet shoot was MS media supplemented 30 g/L sacharose, 7,5 g/L agar, 1 g/L NAA, 2 mg/L IBA and 45 ppm chitooligosaccharide. Supplementation of chitooligosaccharide at concentration of 45 ppm was optimal for the growth of Piper nigrum plantlets both in vitro and ex vitro. Present study indicated that chitooligosaccharide strongly promote the growth of Piper nigrum and recommend concentration for both in vitro and ex vitro is 45 ppm.

scholarly journals Physical Environment in vitro Affects Laboratory and Nursery Growth of Micropropagated Hostas

2000 ◽  
Vol 10 (4) ◽  
pp. 754-757 ◽  
Author(s):  
J. Adelberg ◽  
M. Kroggel ◽  
J. Toler
Keyword(s):  
Root Growth ◽  
Liquid Culture ◽  
Shoot Growth ◽  
Dry Weight ◽  
Ex Vitro ◽  
Agar Culture ◽  
Ex Vitro Growth ◽  
Weight Analysis ◽  
Outdoor Nursery

Hosta ×hybrid Tratt. `Blue Cadet' and Hosta tokudama Tratt. `Newberry Gold' were micropropagated in shaken liquid culture and on agar media, in conventional vessels and vessels modified for ventilation in vitro. Acclimatization under intermittent mist and growth in an outdoor nursery during the late spring and summer were monitored by dry weight analysis of sample plants every 4 days for a 60-day period (ex vitro growth). Results for `Newberry Gold' were 1) in vitro shoot growth was greater in liquid than agar culture, regardless of vessel; 2) shoots from agar or liquid culture grew at similar rates ex vitro; 3) ex vitro root growth was greater for liquid than agar cultured plants, regardless of vessel type. Results for `Blue Cadet' were 1) in vitro and ex vitro shoot growth was greater in liquid than agar culture regardless of vessel type and 2) ex vitro root growth was greatest for liquid cultured plants from conventional vessels. Ventilated vessels were generally beneficial for agar but not liquid culture. Benefits of liquid culture for micropropagation of Hosta found in vitro are at least maintained and sometimes enhanced during ex vitro growth in the mist bed and nursery.

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