scholarly journals How does medicine Parkon® affect the MPTP-induced oxidation stress and MAO systems of the rats brain

2021 ◽  
pp. 001-004
Author(s):  
Goldstein N ◽  
Kamensky A ◽  
Arshavskaya T ◽  
Goldstein R
Keyword(s):  
Oxidation Stress

Models of plant linear growth in conditions of oxidation stress induced by UV-B radiation

2014 ◽  
pp. 17-25
Author(s):  
I.M. Mikhailenko ◽  
◽  
E.V. Kanash ◽  
V.N. Timoshin ◽  
◽  
...  
Keyword(s):  
Linear Growth ◽  
Oxidation Stress

Electrochemical Study of DNA Damaged by Oxidation Stress

2013 ◽  
Vol 16 (2) ◽  
pp. 130-141
Author(s):  
Ondrej Zitka ◽  
Sona Krizkova ◽  
Sylvie Skalickova ◽  
Pavel Kopel ◽  
Petr Babula ◽  
...  
Keyword(s):  
Electrochemical Study ◽  
Oxidation Stress

scholarly journals Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-11
Author(s):  
Shinobu Ito ◽  
Tomohisa Mori ◽  
Hideko Kanazawa ◽  
Toshiko Sawaguchi
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Spin Trap ◽  
Detection Sensitivity ◽  
Mouse Tissue ◽  
Spin Adduct ◽  
Simple Method ◽  
Oxidation Stress ◽  
Spin Resonance

Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗in liver (y=113.8–40.7 log (day),R1=-0.779,R2=0.6,P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.

Oxidative DNA damage and subclinical hypothyroidism in children with obesity

2021 ◽  
Vol 69 (1) ◽  
Author(s):  
Inass Hassan Ahmad ◽  
Marwa khairy Abd Elwahab ◽  
Mervat El Shahat El Wakeel ◽  
Mohamed A. M. Kamal ◽  
Marwa Elhady
Keyword(s):  
Dna Damage ◽  
Lipid Profile ◽  
Thyroid Function ◽  
Subclinical Hypothyroidism ◽  
Central Obesity ◽  
Oxidative Dna Damage ◽  
Anthropometric Measurement ◽  
Thyroid Peroxidase ◽  
Obese Children ◽  
Oxidation Stress

Abstract Background Obesity-related oxidation stress plays a key role in obesity complications; however, its relation to thyroid status is an area for further research. The study aimed to assess thyroid function in obese children and its relation to oxidative deoxyribonucleic acid (DNA) damage. Results Fifty obese and 40 normal weight children were included. Anthropometric measurement, lipid profile, thyroid function, anti-thyroglobulin antibody, thyroid peroxidase antibody, and 8-hydroxydeoxyguanosine serum level as marker of oxidative DNA damage were measured. Thirty-six percent of children with obesity have subclinical hypothyroidism. Central obesity but not oxidative DNA damage and lipid profile was significantly associated with subclinical hypothyroidism. Waist circumference > 97th centile increases the risk for subclinical hypothyroidism (odd ratio 10.82; confidence interval 95% 2.75–42.409; p-value<0.001). Conclusion Central obesity represents a risk factor for subclinical hypothyroidism in obese children. Oxidation DNA damage did not show significant association with subclinical hypothyroidism.

Apocynin attenuates patulin-induced cytotoxicity through reduction of oxidation stress and apoptosis in HEK293cells

2020 ◽  
pp. 1-10
Author(s):  
J.H. Han ◽  
Y.J. Zhong ◽  
C.N. Jin ◽  
R.L. Luo ◽  
M.Y. Xia ◽  
...  
Keyword(s):  
Cell Injury ◽  
Reduced Glutathione ◽  
Hek293 Cells ◽  
Oxygen Species ◽  
Human Embryonic Kidney ◽  
Oxidation Stress ◽  
Mitochondria Membrane Potential ◽  
Alternative Intervention ◽  
Lactate Dehydrogenase Release ◽  
Derivative Products

Patulin (PAT) is a natural mycotoxin that commonly contaminates fruits and their derivative products and has been proven to induce cytotoxicity and oxidative damage in renal cells. In the present study, we aimed to evaluate the effect of apocynin, a potent phenolic antioxidant isolated from plants, on PAT-induced cell injury in human embryonic kidney (HEK293) cells. Compared with 7.5 μM PAT treatment alone, 10 μM apocynin co-treatment elevated cell viability, alleviated lactate dehydrogenase release and reduced caspase activities. Furthermore, apocynin inhibited reactive oxygen species overproduction, re-established mitochondria membrane potential and elevated intracellular ATP content. In addition, the results showed that apocynin aggrandized reduced glutathione (GSH) content, reduced oxidized glutathione (GSSG) content, raised the GSH/GSSG ratio and elevated superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase activities. Collectively, results of the study clearly show that apocynin supplement may serve as an alternative intervention to protect HEK293 cells against cytotoxicity induced by PAT through reduction of oxidation stress and apoptosis.

scholarly journals Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug

2017 ◽  
Vol 7 (4) ◽  
pp. 536-541 ◽  
Author(s):  
M. Khariv ◽  
B. Gutyj ◽  
N. Ohorodnyuk ◽  
O. Vishchur ◽  
I. Khariv ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
Relative Number ◽  
The Body ◽  
T And B Lymphocytes ◽  
Oxidation Stress ◽  
Liposomal Preparation ◽  
Experimental Group

The results of research on the influence of the elaborated complex immunotropic drug containing butafosfan, interferon, thistle and fat-soluble vitamins A, D3, E in the form of a liposomal emulsion on the activity of T- and B-cell chains of immunity in rats under the conditions of action on the body of oxidative stress are presented. It has been established that the introduction of 50% tetrachloromethane into the rats of the first and second experimental groups, with a dose of 0.25 ml per 100 g of body weight, causes oxidative stress in them which negatively affects the cellular immunity and functional activity of immunocompetent blood cells. Immunosuppressive effects of oxidative stress were manifested by a decrease in the blood of rats in the first and second experimental groups of the number of T- and B-lymphocytes and their regulatory subpopulations mainly on the 2nd and 5th day of the study. At the same time, in the blood of rats of the first experimental group in all research periods a decrease in the relative number of common, active and theophylline-resistant T-lymphocytes, as well as B-lymphocytes was observed with a noticeable increase in the number of their undifferentiated forms. At the same time, the obtained data suggest the positive effect of butafosfan, interferon, thistle and vitamins A, D3, E in the liposomal preparation on the relative amount of T- and B-lymphocytes and on the redistribution of avidity in the direction of strengthening the receptor field of plasma membranes immunocompetent cells. It was found that the normalization on the 2nd day of blood level in the second experimental group of common T-lymphocytes occurred due to the secondary forms of the blood and active T-lymphocytes by changes in the number of low-avid forms. In addition to the indicators characterizing the cellular immunity of rats, the components of the liposomal preparation showed regulatory influence on the humoral link of the immune response. In particular, on the 10th day of research in blood of rats of the second experimental group a tendency towards an increase in the relative number of B-lymphocytes and an increase in the number of cells with low and medium density of receptors was found, which, under the conditions of oxidative stress, indicates an increase in the body's ability to actively synthesize protective antibodies

STABILITY INDICATING ASSAY METHOD OF SIROLIMUS USING HPLC AND SEPARATION OF ACID DEGRADANT BY HPTLC

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (04) ◽  
pp. 48-55
Author(s):  
S. Jadhav ◽  
◽  
P. Pisal ◽  
M. Mahajan
Keyword(s):  
Hplc Method ◽  
Assay Method ◽  
Array Detector ◽  
Oxidation Stress ◽  
Stability Indicating ◽  
Rp Hplc ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
The Given ◽  
Stressed Sample

A stability indicating RP-HPLC method has been developed and subsequently validated for Sirolimus. The proposed RP-HPLC method utilizes Phenomenex, C18, 3 μm, 4 mm x 150 mm column, mobile phase consisting of acetonitrile and water (65:35 V/V) and UV detection at 277 nm using a photodiode array detector in the stressed sample chromatograms. Crushed sirolimus tablets were exposed to thermal, photolytic, aqueous and oxidation stress conditions and stressed samples were analysed by the proposed method. Peak homogeneity data of the drug peaks were obtained using photodiode array detector. The stressed sample chromatograms demonstrated the specificity of the method for their estimation in presence of degradants. 99.66% degradation was observed in acid degradation study. on the other hand, no degradation was observed in aqueous condition. The given method was linear over a range of 0.1566 mg/mL to 0.4699 mg/mL. The mean recovery was found to be 99.23%. Acid degradant was separated by HPTLC and spectroscopic analysis was performed for the same.

Oxidation stress and dyslipidemia in pathogenesis of combined craneal and brain injury

2016 ◽  
pp. 127-130
Author(s):  
L.V. Boyarinova ◽  
G.A. Boyarinov ◽  
O.D. Solovieva ◽  
O.V. Voennov ◽  
A.V. Deryugina ◽  
...  
Keyword(s):  
Brain Injury ◽  
Oxidation Stress
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