Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT) v1 (protocols.io.jnxcmfn)

protocols.io ◽  
2017 ◽  
Author(s):  
not provided not provided
Keyword(s):  
Amino Acid ◽  
Canonical Amino Acid

scholarly journals Reassignment of a rare sense codon to a non-canonical amino acid inEscherichia coli

2015 ◽  
Vol 43 (16) ◽  
pp. 8111-8122 ◽  
Author(s):  
Takahito Mukai ◽  
Atsushi Yamaguchi ◽  
Kazumasa Ohtake ◽  
Mihoko Takahashi ◽  
Akiko Hayashi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Inescherichia Coli ◽  
Canonical Amino Acid ◽  
Sense Codon

scholarly journals Synthesis and Evaluation of Cyclic Acetals of Serine Hydroxylamine for Amide-Forming KAHA Ligations

Synthesis ◽  
2019 ◽  
Vol 51 (05) ◽  
pp. 1273-1283 ◽  
Author(s):  
Simon Baldauf ◽  
Jeffrey Bode
Keyword(s):  
Amino Acid ◽  
Amino Acid Residue ◽  
Amino Acid Residues ◽  
High Demand ◽  
Cyclic Acetals ◽  
Ligation Product ◽  
Canonical Amino Acid ◽  
The Stability ◽  
Peptide Segments ◽  
Derivatives Of

The α-ketoacid–hydroxylamine (KAHA) ligation allows the coupling of unprotected peptide segments. The most widely used variant employs a 5-membered cyclic hydroxylamine that forms a homoserine ester as the primary ligation product. While very effective, monomers that give canonical amino acid residues are in high demand. In order to preserve the stability and reactivity of cyclic hydroxylamines, but form a canonical amino acid residue upon ligation, we sought to prepare cyclic derivatives of serine hydroxylamine. An evaluation of several cyclization strategies led to cyclobutanone ketals as the leading structures. The preparation, stability, and amide-forming ligation of these serine-derived ketals are described.

Recombinant hBMP4 incorporated with non-canonical amino acid for binding to hydroxyapatite

2011 ◽  
Vol 33 (9) ◽  
pp. 1885-1890 ◽  
Author(s):  
Makoto Sakuragi ◽  
Takashi Kitajima ◽  
Teruyuki Nagamune ◽  
Yoshihiro Ito
Keyword(s):  
Amino Acid ◽  
Canonical Amino Acid

scholarly journals Complementary techniques to analyse pericellular matrix formation by human MSC within hyaluronic acid hydrogels

2020 ◽  
Vol 1 (8) ◽  
pp. 2888-2896
Author(s):  
Christoph Salzlechner ◽  
Anders Runge Walther ◽  
Sophie Schell ◽  
Nicholas Groth Merrild ◽  
Tabasom Haghighi ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Amino Acid ◽  
Cell Encapsulation ◽  
Pericellular Matrix ◽  
Encapsulated Cells ◽  
The Matrix ◽  
Canonical Amino Acid ◽  
Confocal Raman ◽  
Complementary Techniques ◽  
Raman Spectral

Hydrogels are used widely for cell encapsulation to mimic the native ECM. Here, we characterise and visualise the matrix secreted by encapsulated cells by combining fluorescent non-canonical amino acid tagging with confocal Raman spectral imaging.

scholarly journals Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Talia D. Valentini ◽  
Sarah K. Lucas ◽  
Kelsey A. Binder ◽  
Lydia C. Cameron ◽  
Jason A. Motl ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Amino Acid ◽  
Cystic Fibrosis Lung ◽  
Canonical Amino Acid

Site-Specific Modification ofCandida antarcticaLipase B via Residue-Specific Incorporation of a Non-Canonical Amino Acid

2008 ◽  
Vol 19 (6) ◽  
pp. 1127-1131 ◽  
Author(s):  
Sanne Schoffelen ◽  
Mark H. L. Lambermon ◽  
Mark B. van Eldijk ◽  
Jan C. M. van Hest
Keyword(s):  
Amino Acid ◽  
Site Specific ◽  
Specific Modification ◽  
Canonical Amino Acid

scholarly journals Decreased synthesis of ribosomal proteins in tauopathy revealed by non‐canonical amino acid labelling

2019 ◽  
Vol 38 (13) ◽  
Author(s):  
Harrison Tudor Evans ◽  
Joseph Benetatos ◽  
Marloes van Roijen ◽  
Liviu‐Gabriel Bodea ◽  
Jürgen Götz
Keyword(s):  
Amino Acid ◽  
Ribosomal Proteins ◽  
Canonical Amino Acid
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