Talaromyces (Penicillium) marneffei and Mycobacterium tuberculosis coinfection in a HIV negative patient in Amritsar Punjab, India

Talaromyces marneffei is a rare dimorphic pathogenic fungus that can induce severe infections in human immunodeficiency virus (HIV)-infected patients. However, such infections have also been reported in non-HIV hosts. This current case report describes a very rare case of a T. marneffei pulmonary infection in an HIV-negative patient with a mutation in the tuberous sclerosis complex subunit 2 ( TSC2) gene. A 24-year-old male patient presented with cough and expectoration for 6 months. Computed tomography showed multiple ground-glass opacities and cystic and cavitated lesions in both lungs. Next generation sequencing (NGS) of the bronchoalveolar lavage fluid was performed to confirm T. marneffei pulmonary infection. The results were further verified using bronchoscopy specimen cultures. This was an HIV-negative patient without a travel history to endemic zones and his blood exon sequencing results showed a mutation in the TSC2 gene. To date, he has recovered well with voriconazole therapy. In summary, patients with TSC2 mutations that induce bronchopulmonary dysplasia may be potential hosts for T. marneffei. Early and timely diagnosis is important for improving prognosis. NGS plays a critical role in the diagnosis of T. marneffei pulmonary infection.

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Severe Paradoxical Reaction Requiring Tracheostomy in a Human Immunodeficiency Virus (HIV)-negative Patient with Cervical Lymph Node Tuberculosis

Yonsei Medical Journal ◽

10.3349/ymj.2008.49.5.853 ◽

2008 ◽

Vol 49 (5) ◽

pp. 853 ◽

Cited By ~ 3

Author(s):

In-Suh Park ◽

Dongwook Son ◽

Chanwoo Lee ◽

Jae Eun Park ◽

Jin-Soo Lee ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Lymph Node ◽

Cervical Lymph Node ◽

Paradoxical Reaction ◽

Negative Patient ◽

Immunodeficiency Virus ◽

Lymph Node Tuberculosis ◽

Hiv Negative

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P1619 Unusual association of tuberculous meningitis and cerebral toxoplasmosis in a HIV-negative patient. Case report

International Journal of Antimicrobial Agents ◽

10.1016/s0924-8579(07)71458-9 ◽

2007 ◽

Vol 29 ◽

pp. S455-S456

Author(s):

A. Slavcovici ◽

C. Marcu ◽

M. Lupse ◽

C. Itu ◽

D. Carstina

Keyword(s):

Case Report ◽

Tuberculous Meningitis ◽

Cerebral Toxoplasmosis ◽

Patient Case ◽

Negative Patient ◽

Unusual Association ◽

Hiv Negative

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Identification of RD5-EncodedMycobacterium tuberculosisProteins As B-Cell Antigens Used for Serodiagnosis of Tuberculosis

Clinical and Developmental Immunology ◽

10.1155/2012/738043 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 5

Author(s):

Miao-Miao Zhang ◽

Jun-Wei Zhao ◽

Zhan-Qiang Sun ◽

Jun Liu ◽

Xiao-Kui Guo ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Detection Sensitivity ◽

Comparative Genomic ◽

Healthy Controls ◽

Specific Diagnosis ◽

Pulmonary Tb ◽

Genomic Studies ◽

Immunodominant Antigens ◽

Genomic Regions ◽

Hiv Negative

Comparative genomic studies have identified severalMycobacterium tuberculosis-specific genomic regions of difference (RDs) which are absent in the vaccine strains ofMycobacterium bovisBCG and which may be useful in the specific diagnosis of tuberculosis (TB). In this study, all encoded proteins from DNA segment RD5 ofMycobacterium tuberculosis, that is, Rv3117–Rv3121, were recombined and evaluated by enzyme-linked immunosorbent assays for antibody reactivity with sera from HIV-negative pulmonary TB patients (n=60) and healthy controls (n=32). The results identified two immunodominant antigens, that is, Rv3117 and Rv3120, both of which revealed a statistically significant antigenic distinction between healthy controls and TB patients (P<0.05). In comparison with the well-known early-secreted antigen target 6 kDa (ESAT-6) (sensitivity 21.7%, specificity 90.6%), the higher detection sensitivity and higher specificity were achieved (Rv3117: sensitivity 25%, specificity 96.9%; Rv3120: sensitivity 31.7%, specificity 96.9%). Thus, the results highlight the immunosensitive and immunospecific nature of Rv3117 and Rv3120 and indicate promise for their use in the serodiagnosis of TB.

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