scholarly journals Evaluation of Three Non-Invasive Pregnancy Diagnosis Tests (Modified Seed Germination Inhibition Test, Urine Barium Chloride Test and Milk Copper Sulphate Test) in Buffalo

2020 ◽  
Vol 8 (11) ◽  
Author(s):  
Seikh Masudur Rahman ◽  
Shib Shankar Saha
Keyword(s):  
Seed Germination ◽  
Copper Sulphate ◽  
Barium Chloride ◽  
Inhibition Test ◽  
Germination Inhibition ◽  
Pregnancy Diagnosis ◽  
Non Invasive

scholarly journals Non-invasive Pregnancy Diagnosis from Urine by the Cuboni Reaction and the Barium Chloride Test in Donkeys (Equus asinus) and Alpacas (Vicugna pacos)

2016 ◽  
Vol 19 (3) ◽  
pp. 477-484 ◽  
Author(s):  
A. Kubátová ◽  
T. Fedorova ◽  
I. Skálová ◽  
L. Hyniová
Keyword(s):  
Chloride Solution ◽  
Barium Chloride ◽  
Reproductive Status ◽  
Urine Samples ◽  
Pregnancy Diagnosis ◽  
Non Invasive ◽  
Equus Asinus ◽  
Vicugna Pacos ◽  
Barium Chloride Solution ◽  
Veterinary Institute

Abstract The aim of the research was to evaluate two chemical tests for non-invasive pregnancy diagnosis from urine, the Cuboni reaction and the barium chloride test, in donkeys (Equus asinus) and alpacas (Vicugna pacos). The research was carried out from April 2013 to September 2014. Urine samples were collected on five private Czech farms from 18 jennies and 12 alpaca females. Urine was collected non-invasively into plastic cups fastened on a telescopic rod, at 6-9 week intervals. In total, 60 and 54 urine samples from alpacas and jennies, respectively, were collected. The Cuboni reaction was performed by the State Veterinary Institute Prague. The barium chloride test was done with 5 ml of urine mixed together with 5 ml of 1% barium chloride solution. Results of the Cuboni reaction were strongly influenced by the reproductive status of jennies; the test was 100% successful throughout the second half of pregnancy. However, no relationship was found between the real reproductive status of alpaca females and results of the Cuboni reaction. It was concluded that the barium chloride test is not suitable for pregnancy diagnosis either in donkeys, due to significant influence of season on the results, or in alpacas, because no relationship between results of the test and the reproductive status of alpaca females was found. In conclusion, the Cuboni reaction has potential to become a standard pregnancy diagnostic method in donkeys.

An Alternative Buffalo Urine-based Non-invasive Early Estrus Test using Wheat and Mung Bean Seed Germination

2020 ◽  
Author(s):  
Tanvi Bhatia ◽  
Varij Nayan ◽  
Rakshita Singh ◽  
Chhama Singh ◽  
Anuradha Bhardwaj ◽  
...  
Keyword(s):  
Seed Germination ◽  
Shoot Length ◽  
Urine Samples ◽  
Distilled Water ◽  
Bean Seed ◽  
Germination Inhibition ◽  
Non Invasive ◽  
Buffalo Urine ◽  
First Time ◽  
Wheat Seeds

Background: The silent estrus problem in buffaloes is one of the major bottlenecks in buffalo management. Here, we present for the first time a simple and urine-based non-invasive seed germination inhibition test to detect the early estrus in buffaloes.Methods: The urine samples were collected from buffaloes on 0, 6, 10, 14, 18, 21 and 28 days after detection of signs of heat. The mungbean and wheat seeds (n=15 each) were treated with diluted (1:2 and 1:4) and undiluted urine samples. The results were analyzed in terms of germination inhibition percentage (GI%) and shoot length (SL) measurements. Control was established using distilled water in place of urine.Conclusion: In 1:4 dilution urine samples, a significant (P ≤ 0.05) decrease in GI% and SL were observed after 48 hours and 5 days, respectively in both the seeds, which could be useful as an estrus test. No significant results were observed for GI% and SL with undiluted and 1:2 diluted samples in both seeds. Our findings demonstrate that GI% and SL were inhibited around peri-estrus events, increased from 10th-14th days and then gradually decreased with 1:4 dilution of urine. This estrus test may assist the farmers in timing AI.

scholarly journals DOP06 Non-invasive identification of adherent-invasive E. coli in patients with Crohn’s disease

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S044-S045 ◽  
Author(s):  
A Buisson ◽  
E Vazeille ◽  
X Hébuterne ◽  
M Fumery ◽  
B Pariente ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Unmet Need ◽  
Inhibition Test ◽  
Invasive Ability ◽  
Ileal Mucosa ◽  
Multi Centre Study ◽  
E Coli ◽  
Non Invasive ◽  
Adhesion Inhibition

Abstract Background Medications limiting the adhesion of ‘adherent and invasive E. coli’ (AIEC) represent potential strategies to treat Crohn’s disease (CD). However, the ileal AIEC identification is a time-consuming procedure, and the number of AIEC strains which colonise ileal CD mucosa remains unknown. There is an unmet need for non-invasive biomarkers to identify patients colonised by AIEC. We aimed to evaluate non-invasive biomarker of ileal AIEC colonisation in patients with CD. Methods This prospective and multi-centre study included CD patients requiring ileocoloscopy. Saliva, serum, stools and ileal biopsies were collected. Abundance and global invasive ability of ileal or faecal E. coli were performed. Isolated E. coli were characterised as AIEC or non-AIEC on I407 epithelial cells and THP1 macrophages. The ERIC-PCR profiles of ileal E. coli were performed. Ileal E. coli/CEACAM6 interaction was analysed by a yeast aggregation test and T84 assays (CEACAM6 protein expression, adhesion inhibition test with D-mannose). Quantification of serum anti-E. coli and ileal or salivary CEACAM6 was realised by ELISA. Results Overall, 102 CD patients were enrolled in this study and 25.8% of them exhibited ileal AIEC colonisation (AIEC+). The abundance and global invasive ability of ileal mucosa-associated E. coli were higher in AIEC+ CD patients compared with CD patients without AIEC (AIEC−) (p = 0.0065 and p = 0.0007, respectively). There was no difference between faecal abundance and invasive ability of E. coli between AIEC+ and AIEC− patients. The ERIC-PCR profiles of ileal E. coli showed that CD AIEC+ were for 78% of them colonised by not more than 2 clonal AIEC strains. In addition, AIEC were able to interact with CEACAM6 by binding D-mannose residues and to induce CEACAM6 expression in T84 cells (p = 0.0009 and p = 0.0185, vs. non-AIEC; respectively). This was also supported by adhesion inhibition test. Serum anti-E. coli level was higher for CD AIEC+ (vs. CD AIEC-). Ileal CEACAM6 level were positively correlated with abundance of ileal associated E. coli in AIEC+ patients (r = 0.4000; p = 0.0362) and with salivary CEACAM6 level (r = 0.4690; p < 0.0001). The non-invasive biomarker ‘serum anti-E.coli/salivary CEACAM6’ index was higher for CD AIEC+ (p = 0.0174; vs. CD AIEC-). A cut-off value < 1.34 × 10−6 eliminated the presence of ileal AIEC with a high negative predictive value (90% CI95% [69%–95%]). Conclusion Our study reported that identification of faecal AIEC cannot replace identification of AIEC from ileal biopsies, most of AIEC infection are mono or bi-clonal (≤ 2 strains) and that non-invasive biomarker such as ‘serum anti-E.coli/salivary CEACAM6’ index could be helpful to screen CD patients for AIEC infection.

Evaluation of a rosette inhibition test for pregnancy diagnosis in pigs

1985 ◽  
Vol 7 (2) ◽  
pp. 129-138 ◽  
Author(s):  
A.S. Grewal ◽  
A.L.C. Wallace ◽  
Y.S. Pan ◽  
N.W. Rigby ◽  
J.B. Donnelly ◽  
...  
Keyword(s):  
Inhibition Test ◽  
Pregnancy Diagnosis
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