scholarly journals Identification of mink (Neovison vison) fecal proteins during embryonic diapause and placental pregnancy for non-invasive pregnancy diagnosis in wildlife

2020 ◽  
Author(s):  
E Curry ◽  
JS Easley ◽  
J Wojtusik ◽  
TL Roth
Keyword(s):  
Neovison Vison ◽  
Embryonic Diapause ◽  
Pregnancy Diagnosis ◽  
Non Invasive

scholarly journals Non-invasive identification of protein biomarkers for early pregnancy diagnosis in the cheetah (Acinonyx jubatus)

PLoS ONE ◽  
2017 ◽  
Vol 12 (12) ◽  
pp. e0188575 ◽  
Author(s):  
Diana C. Koester ◽  
David E. Wildt ◽  
Morgan Maly ◽  
Pierre Comizzoli ◽  
Adrienne E. Crosier
Keyword(s):  
Early Pregnancy ◽  
Protein Biomarkers ◽  
Acinonyx Jubatus ◽  
Pregnancy Diagnosis ◽  
Non Invasive

scholarly journals Saliva Crystallization in Cattle: New Possibility for Early Pregnancy Diagnosis?

2013 ◽  
Vol 46 (3) ◽  
pp. 102-104 ◽  
Author(s):  
Iva Skalova ◽  
Tamara Fedorova ◽  
Karolina Brandlova
Keyword(s):  
Early Pregnancy ◽  
Artificial Insemination ◽  
Low Cost ◽  
Oestrous Cycle ◽  
Holstein Cows ◽  
Practical Application ◽  
Synchronization Process ◽  
Pregnancy Diagnosis ◽  
Non Invasive

Abstract Saliva sampling is a non-invasive, simple and low-cost procedure. The aim of this study was to confirm the presence and changes of saliva crystallization in domestic cattle during synchronized oestrous cycle and early pregnancy. We verified saliva crystallization as a method for early pregnancy diagnosis. Eight Holstein cows were included into the research. The samples were collected daily from 16th day before to 34th day after artificial insemination (in total 51 days). We observed the following types of crystallization: none, dotted, branch-like, fir-like, fern-like and combinations of them and an atypical pattern. We confirmed the presence of saliva crystallization in cattle and its changes during oestrus synchronization process, insemination and post-insemination periods. We found significant differences in pregnant and non-pregnant animals between 20th and 29th day after insemination. We concluded that pregnancy diagnosis by saliva crystallization might be possible but the practical application of this method is currently unfeasible.

scholarly journals Método no invasivo de diagnóstico de gestación en venadas Odocoileus virginianus. (Non-Invasive Method for Pregnancy Diagnosis in Odocoileus Virginianus.)

2015 ◽  
Vol 6 (1) ◽  
pp. 9 ◽  
Author(s):  
P. Cervera Hernández ◽  
R. C. Montes Pérez ◽  
F. Victoria Arceo ◽  
J. Camacho Reyes ◽  
I. F. Castillo López
Keyword(s):  
Odocoileus Virginianus ◽  
Reproductive Season ◽  
Pregnancy Diagnosis ◽  
Non Invasive ◽  
Total Correlation ◽  
In Captivity ◽  
Invasive Method

ResumenObjetivo: Valorar la eficiencia del diagnóstico de gestación mediante la estimación de niveles de progestinas fecales en venadas cola blanca O. virginianus en la época no reproductiva. Materiales y métodos: Se analizaron muestras sanguíneas y heces de nueve venadas cola blanca (VCB), semanalmente, durante dos meses en la época reproductiva. Adicionalmente, las VCB fueron expuestas a macho durante un mes durante la época reproductiva, y se colectaron heces dos veces fuera de esta, para medir los niveles de metabolitos de progestinas. Se determinó el valor de corte para diagnosticar gestación a partir de los niveles de metabolitos de progestinas fecales en fase luteal y folicular. La progesterona sanguínea fue medida por radioinmunoanálisis, y se correlacionó con los niveles de progestinas fecales. Los diagnósticos de gestación fueron relacionados con los partos. Resultados: La correlación total entre niveles de progestinas sanguíneas y metabolitos de progestinas fecales fue 0,37 (p&lt,0,01). La variación de la correlación individual fue 0,104 hasta 0,99. El valor de corte para diagnosticar gestación fue 4000 ng/g en heces. La eficiencia del diagnóstico de gestación fue 77,78%, el valor predictivo del diagnóstico positivo fue 75%, y el negativo fue 80%, respectivamente. Conclusiones: Es posible diagnosticar gestación fuera de la época de reproducción mediante la estimación de los niveles de metabolitos de progestinas fecales, en VCB en cautiverio, tomando como valor de corte 4000 ng/g de heces. AbstractObjective: To value the eciency of pregnancy diagnosis by estimating levels of fecal progestins inwhite-tailed deer O. virginianus outside the reproductive season. Materials and methods: Blood and feces samples of nine White tailed-deer (WTD) were analyzed weekly for two months in reproductive season. Additionally WTD were exposed to a male during a month in reproductive season and feces were collected twice in non-reproductive season, in order to estimate progestins metabolites levels. The cut value for pregnancy diagnosis from levels of fecal progestins metabolites in luteal and follicular phases of WTD, was determined. Blood progesterone levels were measured by radioimmuno analysis and were correlated with the fecal progestins levels. Pregnancy diagnosis was related with the deliveries. Results: Total correlation coecient between blood progestins levels and fecal metabolites progestins was 0.37 (p&lt,0.01). Individual correlation coecient variation was 0.104 to 0.99. Cut o value for pregnancy diagnosis was 4000 ng/g in feces. Eciency for pregnancy diagnosis was 77.78%, predictive positive value was 75% and the negative was 80% respectively. Conclusions: It is possible to carry out pregnancy diagnosis in non-reproductive season, through quantification of fecal progestin metabolites levels, in WTD in captivity, taking as cut value 4000 ng/g feces.

scholarly journals Non-Invasive Pregnancy Diagnosis in Big Cats using the PGFM (13,14-dihydro-15-keto-PGF2α) Assay

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0143958 ◽  
Author(s):  
Martin Dehnhard ◽  
Vinod Kumar ◽  
Mithileshwari Chandrasekhar ◽  
Katarina Jewgenow ◽  
Govindhaswamy Umapathy
Keyword(s):  
Pregnancy Diagnosis ◽  
Non Invasive

scholarly journals Non-invasive Pregnancy Diagnosis from Urine by the Cuboni Reaction and the Barium Chloride Test in Donkeys (Equus asinus) and Alpacas (Vicugna pacos)

2016 ◽  
Vol 19 (3) ◽  
pp. 477-484 ◽  
Author(s):  
A. Kubátová ◽  
T. Fedorova ◽  
I. Skálová ◽  
L. Hyniová
Keyword(s):  
Chloride Solution ◽  
Barium Chloride ◽  
Reproductive Status ◽  
Urine Samples ◽  
Pregnancy Diagnosis ◽  
Non Invasive ◽  
Equus Asinus ◽  
Vicugna Pacos ◽  
Barium Chloride Solution ◽  
Veterinary Institute

Abstract The aim of the research was to evaluate two chemical tests for non-invasive pregnancy diagnosis from urine, the Cuboni reaction and the barium chloride test, in donkeys (Equus asinus) and alpacas (Vicugna pacos). The research was carried out from April 2013 to September 2014. Urine samples were collected on five private Czech farms from 18 jennies and 12 alpaca females. Urine was collected non-invasively into plastic cups fastened on a telescopic rod, at 6-9 week intervals. In total, 60 and 54 urine samples from alpacas and jennies, respectively, were collected. The Cuboni reaction was performed by the State Veterinary Institute Prague. The barium chloride test was done with 5 ml of urine mixed together with 5 ml of 1% barium chloride solution. Results of the Cuboni reaction were strongly influenced by the reproductive status of jennies; the test was 100% successful throughout the second half of pregnancy. However, no relationship was found between the real reproductive status of alpaca females and results of the Cuboni reaction. It was concluded that the barium chloride test is not suitable for pregnancy diagnosis either in donkeys, due to significant influence of season on the results, or in alpacas, because no relationship between results of the test and the reproductive status of alpaca females was found. In conclusion, the Cuboni reaction has potential to become a standard pregnancy diagnostic method in donkeys.

X-ray microtomography

1988 ◽  
Vol 46 ◽  
pp. 998-999
Author(s):  
H.W. Deckman ◽  
B.F. Flannery ◽  
J.H. Dunsmuir ◽  
K.D' Amico
Keyword(s):  
Computed Tomography ◽  
Internal Structure ◽  
Imaging Technique ◽  
Three Dimensional ◽  
Tissue Structure ◽  
Individual Element ◽  
X Ray ◽  
Non Invasive ◽  
Panoramic Imaging ◽  
Three Dimensional Images

We have developed a new X-ray microscope which produces complete three dimensional images of samples. The microscope operates by performing X-ray tomography with unprecedented resolution. Tomography is a non-invasive imaging technique that creates maps of the internal structure of samples from measurement of the attenuation of penetrating radiation. As conventionally practiced in medical Computed Tomography (CT), radiologists produce maps of bone and tissue structure in several planar sections that reveal features with 1mm resolution and 1% contrast. Microtomography extends the capability of CT in several ways. First, the resolution which approaches one micron, is one thousand times higher than that of the medical CT. Second, our approach acquires and analyses the data in a panoramic imaging format that directly produces three-dimensional maps in a series of contiguous stacked planes. Typical maps available today consist of three hundred planar sections each containing 512x512 pixels. Finally, and perhaps of most import scientifically, microtomography using a synchrotron X-ray source, allows us to generate maps of individual element.

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