scholarly journals Effect of Tea Leaves Powder Supplementation on Fermented Oil Palm Fronds on Fermentation Characteristics, Rumen Microbial Profile, and Methane Production In vitro

Author(s):  
Nurhaita Nurhaita ◽  
Nur Hidayah
2021 ◽  
Vol 19 (1) ◽  
pp. 13
Author(s):  
Nurhaita Nurhaita ◽  
Nur Hidayah ◽  
Neli Definiati

<p class="MDPI17abstract"><strong>Objective: </strong>This research was aimed to evaluation the effect of supplementation jengkol peel powder in fermented oil palm fronds on rumen microbes profile and methane production on <em>in vitro</em>.</p><p class="MDPI17abstract"><strong>Methods: </strong>The used design was factorial randomized block design with four treatments supplementation of jengkol peel powder at 0, 2, 4, and 6% and four groups as replications. The observed variables were rumen microbes profile (population of total bacteria, cellulolytic, and protozoa), methane production, partial <em>volatile fatty acid</em> (VFA) proportion and acetate/propionate (A/P) ratio. The data were analyzed using ANOVA and different among treatments means examined by Duncan Multiple Range Test (DMRT).<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The result showed that supplementation of jengkol peel powder at 6% substrate increased (P&lt;0.05) total and cellulolytic bacteria population. Jengkol peel powder supplementation as much as 4 and 6% decreased (P&lt;0.05) protozoa population, methane production, acetate proportion and A/P ratio. Supplementation of jengkol peel powder at 2% increased (P&lt;0.05) propionate production.<strong></strong></p><p class="MDPI17abstract"><strong>Conclusions: </strong>In conclusion, fermented oil palm fronds can be substituted with jengkol peel powder at 2% substrate that did not disturb rumen microbes profile, decreased methane production, acetate proportion, A/P ratio and increased propionate production on <em>in vitro</em>.<strong></strong></p>


2020 ◽  
Author(s):  
Ahmad Fariz - Nicholas ◽  
H.A. - Hassim ◽  
A.F. - Nicholas ◽  
Marta - Lourenço ◽  
A.A - Dias ◽  
...  

Abstract Background: Oil palm fronds (OPF) when pretreated with white rot fungi (WRF) shows increased rumen degradability but with significant biomass loss. Thus, effects of pre-treated OPF with enzyme extracts from WRF on rumen degradability were studied in vitro. The enzyme extracts were prepared by inoculating OPF with three WRF, i.e Ceriporiopsis subvermispora, Lentinula edodes and Ganoderma lucidum, for 15, 30 and 45 days with either ammonium sulphate, (NH4)2SO4 and sodium nitrate, NaNO3 added to the culture media for each inoculation period. After preparation of enzyme extracts, the enzyme activities were determined. OPF was then pre-treated with enzyme extracts in a citrate buffer (pH 5.0) in a forced air oven at 40 oC during 5 days. Further, the in vitro rumen degradation of OPF pre-treated with enzyme extracts, with respect to the short chain fatty acid (SCFA) production, was determined after 24 h incubation. Activity of lignolytic (laccase and MnP), cellulolytic (CMCase and avicelase) and hemicellulolytic (xylanase) enzymes were measured in all of the extracts irrespective on the inoculation period. Results: Treatment of OPF with enzyme extracts from G. lucidum after 45 days of inoculation showed a numerical increase (13%) in total SCFA and apparently rumen degradable carbohydrates (ARDC) after 24 h in vitro incubation, without any loss of biomass. However, this increase was not clearly correlated to results of the enzyme assays. Conclusion: This study indicates pre-treatment of OPF with enzyme extracts from specific WRF to be promising to enhance the ruminal degradability of OPF without simultaneous loss of biomass.


2015 ◽  
Vol 15 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Sitti Wajizah ◽  
Samadi Samadi ◽  
Yunasri Usman ◽  
Elmy Mariana

(The evaluation of  nutritive value and In Vitro digestibility of oil palm fronds through fermentation by using  Aspergillus niger  with  different soluble carbohydrate sources) ABSTRACT. Oil palm frond (OPF) is one of potential sources of alternative feed, but has limited use due to high crude fiber and low crude protein contents. Fermentation is one of the methods widely applied to improve nutritive value of animal feed. The purpose of this research is to increase nutritive value of fermented oil palm fronds by adding different soluble carbohydrate source into fermentation media. The results of the experiments indicated that fermented oil palm fronds by Aspergillus niger had a significant effect (P0,05)  on the content of crude protein, crude fiber, and ash. Generally, fermented oil palm fronds with different soluble carbohydrate was able to increase the content of crude protein of oil palm fronds, but not optimal yet in reducing the crude fiber content of fermented substrate. However, the addition of rice bran on the fermentation medium showed the best results, characterized by increasing crude protein and decreasing crude fiber contents as well as improved dry matter and organic matter digestibility, reflected in high concentration of VFA. 


2008 ◽  
Vol 11 (4) ◽  
pp. 583-588 ◽  
Author(s):  
M. Ramin ◽  
A.R. Alimon ◽  
J.M. Panandam ◽  
K. Sijam ◽  
A. Javanmard ◽  
...  

2012 ◽  
Vol 92 (1) ◽  
pp. 79-87 ◽  
Author(s):  
H. A. Hassim ◽  
M. Lourenço ◽  
Y. M. Goh ◽  
J. J. P. Baars ◽  
V. Fievez

Hassim, H. A., Lourenço, M., Goh, Y. M., Baars, J. J. P. and Fievez, V. 2012. Rumen degradation of oil palm fronds is improved through pre-digestion with white rot fungi but not through supplementation with yeast or enzymes. Can. J. Anim. Sci. 92: 79–87. Rumen fermentation kinetics of oil palm fronds (OPF) supplemented or not with enzymes (Hemicell® or Allzyme SSF®) or yeasts (Levucell®SC or Yea-Sacc®) were studied through an in vitro gas production test (96 h) (exp. 1). In exp. 2, enzymes were supplemented to OPF pre-treated during 3 or 9 wk with either one of five white rot fungi strains. Yeasts and enzymes were tested both in active and inactive forms, which revealed the most appropriate set-up to distinguish between the rate of supplements as direct contributors to the fermentation substrate vs. stimulators of the fermentation of the basal substrate. In exp 1, addition of active and inactive Yea-Sacc® increased the apparently rumen degradable carbohydrates (ARDC) by 11%, whereas enzymes did not affect rumen degradability of non-inoculated OPF. Neither yeast nor enzymes influenced the rate of gas production of non-inoculated OPF, except for active Hemicell® at the low dose. In exp. 2, inoculation of OPF with Ceriporiopsis subvermispora for 3 wk and Lentinula edodes for 9 wk increased ARDC, but additional enzyme supplementation did not further improve ARDC or the rate of gas production.


2021 ◽  
Vol 60 (5) ◽  
pp. 2011-2026
Author(s):  
Eng Kein New ◽  
Ta Yeong Wu ◽  
Khai Shing Voon ◽  
Alessandra Procentese ◽  
Katrina Pui Yee Shak ◽  
...  

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