EVALUATION OF THE ROLE OF LIGHT INTENSITY AND HORMONAL TREATMENTS DURING IN VITRO ROOTING OF MYRTUS MICROPROPAGATED PLANTLETS RELATED TO ACCLIMATIZATION PERFORMANCES

One of the challenges related to propagation of Arenga pinnata is its lengthy period of seed dormancy. In this study, in vitro regeneration was carried out to determine the effect of hormonal treatment on the embryo explant of Arenga pinnata. Embryos were surface sterilized and cultured into different media supplemented with various hormones concentrations and combinations. Each treatment contained of Kinetin (KN) hormone (1.0, 2.0, and 3.0 mg/l) and in combination with indole-3-acetic acid (IAA) of 0.1, 0.2, 0.3 mg/l. The height of plumule and length of radical was observed and recorded. Treatment 8 (3 mg/ml KN + 0.1 mg/ml IAA) showed 59.09% in plumule height increment while treatment 4 (1 mg/ml KN + 0.3 mg/ml IAA) showed the highest radical increments with 93.62%. The knowledge gained in this study consequently helps us to better understand the role of KN and IAA in the in vitro regeneration protocol. Since in vitro method able to produce higher number of in vitro seedlings at one time, it is important to establish the in vitro regeneration protocol for this plant.

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Etude Des Effets Des Régulateurs De Croissance Sur La Les Stades De Prolifération Et De Développement De La Pomme De Terre (Solanum Tuberosum. L) In Vitro

European Scientific Journal ESJ ◽

10.19044/esj.2017.v13n24p145 ◽

2017 ◽

Vol 13 (24) ◽

pp. 145

Author(s):

Sadek Chahredine ◽

Nadia Ykhlef

Keyword(s):

Solanum Tuberosum ◽

Callus Induction ◽

Solanum Tuberosum L ◽

Naphthaleneacetic Acid ◽

Compact Structure ◽

Potato Plants ◽

Régulateurs De Croissance ◽

Role Of Light

The aim of this study is to determine the effects of different concentrations and combinations of the phytohormones, 1-naphthaleneacetic acid (NAA), and 6-benzylaminopurine (BAP): M1 (0.5 mg / l +1 mg / l), M2 (1 mg / l + 0.5 mg / l) , M3 (2 mg / l +2 mg / l), M4 (0.5 mg / l + l mg / l, NAA), M5 (1.0 mg / l + l mg / l , NAA), and M6 (2.0 mg / l + l mg / l, NAA). This study was carried out in dark condition on callus induction of potato plants (Solanum tuberosum L.) cultivars from potato tuber bud so as to demonstrate the role of light. The callus initiation begins after 7 days of incubation for all studied media. After two months of incubation, the better development of callus was noted in Spunta variety by using medium M1, M2, M3, and M6. The calluses took a compact structure of brown-white color for both varieties with a callus induction rate of 20- 40%. This was collected with kondor variety for M2 and (M3, M4, M5) media respectively and 10-30% for M4 (M1, M2, M3) for Spunta variety also. The highest fresh weight was recorded on M2 medium with 0.26g for Kondor variety and 0.93g for Spunta variety.

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Role of light‐intensity‐dependent changes in thiol and amino acid metabolism in the adaptation of wheat to drought

Journal of Agronomy and Crop Science ◽

10.1111/jac.12358 ◽

2019 ◽

Vol 205 (6) ◽

pp. 562-570

Author(s):

Mónika Gyugos ◽

Mohamed Ahres ◽

Zsolt Gulyás ◽

Gabriella Szalai ◽

Éva Darkó ◽

...

Keyword(s):

Amino Acid ◽

Light Intensity ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Role Of Light

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Environmental Influence on Locomotor Activity in Nephrops Norvegicus (Crustacea: Decapoda)

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315400024152 ◽

1980 ◽

Vol 60 (1) ◽

pp. 103-113 ◽

Cited By ~ 22

Author(s):

T. H. Moller ◽

E. Naylor

Keyword(s):

Locomotor Activity ◽

Light Intensity ◽

Environmental Influence ◽

Activity Patterns ◽

Nephrops Norvegicus ◽

Experimental Conditions ◽

Emergence Patterns ◽

Role Of Light ◽

Trawl Catches

Diel variations in the emergence of the burrowing prawn Nephrops norvegicus (L.) have been investigated by direct field observations (Chapman & Rice, 1971; Chapman, Johnstone & Rice, 1975; Chapman & Howard, 1979; Atkinson & Naylor, 1976), and indirectly by sequential trawling during 24 h periods (Höglund & Dybern, 1965; Simpson, 1965; Hillis, 1971; Farmer, 1974; Atkinson & Naylor, 1976; Oakley, 1979). Peak emergence appears to be related to temporal and depth-dependent variations in daylight penetration, since Nephrops are apparently nocturnal in shallow waters, crepuscular as the depth increases, and diurnal at the greatest depths of their occurrence. This lends support to the suggestion that emergence occurs at an optimum light intensity (Hillis, 1971; Chapman, Priestley & Robertson, 1972; Chapman, et al., 1975; Chapman & Howard, 1979). However, additional factors influencing emergence of Nephrops from their burrows have also to be taken in account, since laboratory studies of locomotor activity in Nephrops have consistently revealed nocturnal activity patterns in light-dark (LD) regimes, with light inhibiting locomotor activity even at extremely low irradiance levels (Arechiga & Atkinson, 1975; Atkinson & Naylor, 1973, 1976; Naylor & Atkinson, 1976). Moreover, Hammond & Naylor (1977 a) have presented qualitative evidence that the nocturnal locomotor activity peak appears to be synchronized by falling light intensity at dusk. The differences between these experimental results and emergence patterns deduced from trawl catches and underwater observations of Nephrops have not been fully resolved by studies of the role of light intensity and of gradual light transitions (Arechiga & Atkinson, 1975; Hammond & Naylor, 1977 a, b). Thus the behavioural responses of Nephrops both in the field and in the laboratory need to be assessed in relation to more accurately quantified light changes. Also, despite earlier evaluation of the problem (Atkinson & Naylor, 1976; Hammond & Naylor, 1977a) it is necessary to reconsider the possibility that the patterns of locomotor activity recorded in the laboratory are influenced by experimental conditions, as has been demonstrated for minnows (Jones, 1956), and flatfish (Verheijen & de Groot, 1967).

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