Obtention of tetraploids in bottle gourd (Lagenaria siceraria) and citron (Citrullus lanatus var. citroides) by shoot immersion method using different colchicine doses and application times

2020 ◽  
pp. 357-362
Author(s):  
M.D. Kombo ◽  
N. Sari ◽  
I. Solmaz
Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 3019-3025
Author(s):  
Sean M. Toporek ◽  
Anthony P. Keinath

Pythium species cause root and stem rot in watermelon (Citrullus lanatus), but cucurbit rootstocks used to graft watermelon have not been evaluated for resistance. P. aphanidermatum and P. myriotylum were inoculated onto 15 nongrafted watermelon, citron (Citrullus amarus), bottle gourd (Lagenaria siceraria), and interspecific hybrid squash (Cucurbita maxima × C. moschata) cultivars in a growth chamber. Watermelon was more susceptible than bottle gourd and interspecific hybrid squash at 20 and 30°C. Twenty-one cultivars were inoculated in a field with an equal blend of both Pythium species. Interspecific hybrid squash was less susceptible than bottle gourd and watermelon in 2018 and 2019. Seedless watermelon cultivar Tri-X 313 was grafted to one citron, one bottle gourd, and three interspecific hybrid squash rootstocks. Plants were inoculated in the field as described. Grafting to interspecific hybrid squash rootstocks reduced disease incidence compared with nongrafted controls in 2018 and 2019. Mefenoxam and propamocarb applied at transplanting did not affect disease compared with non-fungicide-treated plots. Grafting to interspecific hybrid squash Camelforce significantly increased total and marketable fruit numbers and total weight in 2019 compared with the nongrafted control. In summary, interspecific hybrid squash was consistently resistant to Pythium, demonstrating resistance and utility in watermelon grafting.


Plant Disease ◽  
2010 ◽  
Vol 94 (10) ◽  
pp. 1195-1199 ◽  
Author(s):  
Judy A. Thies ◽  
Jennifer J. Ariss ◽  
Richard L. Hassell ◽  
Steve Olson ◽  
Chandrasekar S. Kousik ◽  
...  

Four bottle gourd (Lagenaria siceraria) cultivars, one squash (Cucurbita moschata × C. maxima) hybrid, five wild watermelon (Citrullus lanatus var. citroides) germplasm lines, and one commercial wild watermelon (C. lanatus var. citroides) cultivar were evaluated as rootstocks for cultivated watermelon (C. lanatus var. lanatus) in fields infested with the southern root-knot nematode (Meloidogyne incognita) in Charleston, SC in 2007 and 2008, and in Quincy, FL in 2008. Commercial watermelon ‘Fiesta’ (diploid seeded) and ‘Tri-X 313’ (triploid seedless) scions were grafted onto the rootstocks in 2007 and 2008, respectively. In 2007, the plants grafted on rootstock from the wild watermelon germplasm line RKVL 318 had significantly less (P < 0.05) root galling than nongrafted ‘Fiesta’ watermelon or plants with the squash hybrid or bottle gourd rootstocks. In 2008, ‘Fiesta’ plants with rootstocks from all five wild watermelon germplasm lines and the commercial watermelon rootstock had significantly less (P < 0.05) root galling than plants with the squash hybrid or bottle gourd rootstocks. Root galling of the squash hybrid and bottle gourd rootstocks was severe (78 to 99%) in both years. Root galling for nongrafted ‘Fiesta’ and ‘Tri-X 313’ watermelon was 36 and 50%, respectively. Root galling for the wild watermelon germplasm lines ranged from 11 to 34% and 36 to 44% in 2007 and 2008, respectively. Wild watermelon germplasm lines derived from C. lanatus var. citroides were identified that may be useful as resistant rootstocks for managing root-knot nematodes in watermelon.


Plant Disease ◽  
2018 ◽  
Vol 102 (7) ◽  
pp. 1290-1298 ◽  
Author(s):  
Chandrasekar S. Kousik ◽  
Mihir Mandal ◽  
Richard Hassell

Powdery mildew (PM) is a major foliar disease causing serious economic losses of cucurbit crops grown in the United States. The pathogen Podosphaera xanthii, which causes PM, is known to infect seedlings, stems, foliage, petioles, and fruit of cucurbit crops. In recent years, grafting watermelon on resistant rootstocks for managing soilborne diseases has been gaining popularity in the U.S.A. However, grafting for managing foliar diseases has not yet received adequate attention. Three greenhouse experiments were conducted during the summer months of 2012, 2013, and 2014 to determine if PM-resistant rootstocks could impart resistance to a susceptible watermelon scion. Susceptible watermelon scion ‘Mickey Lee’ seedlings were grafted onto 25 watermelon (Citrullus lanatus, C. amarus, C. mucosospermus) and four bottle gourd (Lagenaria siceraria) rootstocks. Grafted plants were inoculated with a 2 × 104 conidia ml−1 suspension of P. xanthii conidia and disease severity was rated 14 days after inoculation. Mickey Lee grafted on six PM-resistant watermelon rootstocks had significantly (P ≤ 0.05) lower PM severity on cotyledons, 2nd true leaf, and upper leaves (rating for 3rd to 7th or 8th true leaf) compared with Mickey Lee grafted on susceptible watermelon USVL677-PMS or bottle gourd USVL848-PMS rootstocks. However, some of the resistant watermelon rootstocks did not impart significant resistance to the scion. The resistant bottle gourd rootstocks USVL482-PMR and USVL351-PMR provided significantly greater levels of resistance, compared with many of the resistant watermelon rootstocks. Grafting watermelon on resistant rootstocks may help mitigate the effects of PM on susceptible scion seedlings.


Agriculture ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 812
Author(s):  
Changjin Liu ◽  
Weiguo Lin ◽  
Chongran Feng ◽  
Xiangshuai Wu ◽  
Xiaohu Fu ◽  
...  

Grafting is a widely used technique in watermelon (Citrullus lanatus) production. However, cost of grafted seedlings is generally high as a result of intensive labor inputs for propagation using traditional grafting methods such as the manual removal of rootstock regrowth. This study developed a new grafting tool to physically remove the epidermis of pumpkin (C. maxima × C. moschata) and bottle gourd (Lagenaria siceraria) rootstock cotyledon base during grafting; we called this a new grafting method. Compared with the traditional grafting, the new grafting method significantly decreased the pumpkin rootstock regrowth rate from 100% to 8% in hole insertion and 2% in one cotyledon grafting, respectively. These attenuated rates for bottle gourd rootstock regrowth were 23% and 9% in hole insertion and one cotyledon grafting, respectively. The scion dry weights of new hole insertion and one cotyledon grafting were increased by 78% and 74% when pumpkin was used as rootstock as compared with traditional grafting without regrown rootstock removal, while the respective values were 33% and 17% in bottle gourd rootstock grafted plants. In addition, the time used for the new hole insertion grafting method to physically remove the epidermis of pumpkin rootstock cotyledon base was significantly shorter than the time required to remove the rootstock regrowth manually three times in the traditional grafting (4.2 s/plant vs. 9.3 s/plant). Similar results were also observed in the new one cotyledon grafting (4.2 s/plant vs. 8.8 s/plant). Taken together, this study presents a new method in watermelon grafting to reduce rootstock regrowth, therefore benefiting both scion growth and plant management, thus the development of this new method is clearly useful in watermelon production.


2011 ◽  
Vol 30 (5) ◽  
pp. 233-236 ◽  
Author(s):  
Rajesh Puri ◽  
Randhir Sud ◽  
Abdul Khaliq ◽  
Mandhir Kumar ◽  
Sanjay Jain

Author(s):  
Muhammad Zahoor ◽  
Muhammad Ikram ◽  
Nausheen Nazir ◽  
Sumaira Naz ◽  
Gaber El-Saber Batiha ◽  
...  

: Herbal remedies have been employed for the treatment and management of different diseases for ages. Herbal medicines are a promising choice over modern synthetic drugs because of their low side effects and are thus considered safe and effective in treating human diseases. Lagenaria siceraria (Mol.) Standley fruit (Bottle gourd) belongs to the Cucurbitaceae family that has been used in a different system of traditional medication to treat various diseases. This is a domestic plant that provides food as well as medication. This vegetable has low caloric values and high water contents. The edible portion of it contains phytochemicals like vitamins, proteins, choline, minerals, terpenoids, flavonoids etc. Several bioactive compounds have been isolated from L. siceraria, including triterpenoids, sterols, cucurbitacins, flavones, C-glycosides and β-glycosides. Researchers have evaluated various parts of this plant viz., fruit, root, flowers, and leaves for pharmacological activities like antianxiety, antidepressant, diuretic, antimicrobial, cytotoxic, antihyperlipidemic, cardioprotective, analgesic, anti-inflammatory, anthelmintic, anti-hyperglycemic, antihepatotoxic, anti-urolithiatic, antistress, antiulcer, anticancer, hepatoprotective, anthelmintic, immunomodulatory, and antioxidant. In this review, an attempt has been made to explore its phytochemical constituents, traditional, medicinal, and pharmacological uses to highlight the therapeutic importance of this well-known plant. This would be helpful in reviving its importance and highlight its several promising aspects to encourage researchers for further research on L. siceraria.


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