Determination of Grafting Adaptation of Some Chestnut (Castanea sativa Mill.) Genotypes with Marigoule (C. Sativa × C. Crenata) Cultivar

In this study, to reproduce natural chestnut genotypes, the fruit characteristics of the Düzce region determined by local people are good in a healthy way, compatible with Marigoule (C. sativa × C. crenata) hybrid varieties resistant to branch cancer and root rot was investigated. In this study, 24 different chestnut genotypes were used. As the grafting method, the most commonly used tongue grafting, split grafting, chip budding grafting, and side grafting method were used. All procedures related to grafting were carried out in the greenhouses of the Düzce University Faculty of Forestry. In this study, local genotypes that are compatible with Marigoule chestnut were determined. As a result, the native genotype, which showed the best adaptation to Marigoule seedlings, was 87.5% of Yalnızçam, and after that, 79.2% of the Ereğli Sefer genotype. The lowest fit showed Broken genotype with 15% and Akçakoca1genotype with 17.5%. Besides, the compatibility of foreign varieties Maraval, Marigoule, and Betizac were also investigated. As a result, Betizac had the highest compatibility with 95%, while Maraval had 67.5%. The most successful in the grafting methods applied was the side graft (74.2%) followed by tongue grafting (59.9%), splitting grafting (51.4%), and chip budding grafting (29.7%).

A New Grafting Method for Watermelon to Inhibit Rootstock Regrowth and Enhance Scion Growth

Grafting is a widely used technique in watermelon (Citrullus lanatus) production. However, cost of grafted seedlings is generally high as a result of intensive labor inputs for propagation using traditional grafting methods such as the manual removal of rootstock regrowth. This study developed a new grafting tool to physically remove the epidermis of pumpkin (C. maxima × C. moschata) and bottle gourd (Lagenaria siceraria) rootstock cotyledon base during grafting; we called this a new grafting method. Compared with the traditional grafting, the new grafting method significantly decreased the pumpkin rootstock regrowth rate from 100% to 8% in hole insertion and 2% in one cotyledon grafting, respectively. These attenuated rates for bottle gourd rootstock regrowth were 23% and 9% in hole insertion and one cotyledon grafting, respectively. The scion dry weights of new hole insertion and one cotyledon grafting were increased by 78% and 74% when pumpkin was used as rootstock as compared with traditional grafting without regrown rootstock removal, while the respective values were 33% and 17% in bottle gourd rootstock grafted plants. In addition, the time used for the new hole insertion grafting method to physically remove the epidermis of pumpkin rootstock cotyledon base was significantly shorter than the time required to remove the rootstock regrowth manually three times in the traditional grafting (4.2 s/plant vs. 9.3 s/plant). Similar results were also observed in the new one cotyledon grafting (4.2 s/plant vs. 8.8 s/plant). Taken together, this study presents a new method in watermelon grafting to reduce rootstock regrowth, therefore benefiting both scion growth and plant management, thus the development of this new method is clearly useful in watermelon production.

EFFICIENCY OF VEGETATIVE PROPAGATION OF CAJÁ-DE-JABUTI UNDER CONCENTRATIONS OF INDOLEBUTYRIC ACID AND GRAFTING METHODS

ABSTRACT The objectives of the tests were to evaluate concentrations of indolebutyric acid (IBA) combined with different types of cuttings and grafting methods on the vegetative propagation of cajazeira-dejabuti (S. testudinis). The experiment with stem cuttings was conducted in a greenhouse with an intermittent misting system. The experimental design was completely randomized in a 5 x 6 factorial arrangement consisting of five cutting types: apical, medial, basal, stratified, and tanchan, and six IBA concentrations: 0, 1,000, 2,000, 3,000, 4,000, and 5,000 mg kg-1, with four replications of ten cuttings. The experiment with the root cuttings followed a randomized block design composed of six IBA concentrations (0, 1,000, 2,000, 3,000, 4,000, and 5,000 mg kg-1) with four replications, evaluating after 120 days the percentages of live, sprouted, rooted, and calloused cuttings, and the number and dry mass of roots and sprouts. Five grafting methods (chip budding, shield budding, cleft grafting, wedge grafting, and splice grafting) were tested by evaluating the graft success and sprouting percentages at 30 and 60 days after grafting, respectively. Statistical analyses were performed using the Kruskal-Wallis non-parametric test. The IBA concentrations of 0, 2,000, 3,000, and 5,000 mg kg-1 increased the number of sprouts in tanchan cuttings. The tanchan cuttings showed the highest survival and percentage of sprouts regardless of the IBA concentration. The IBA concentration of 2,200 mg kg-1 promotes higher rooting rates in root cuttings. Splice grafting provides higher graft success and sprouting rates.

Comparison of Structure and Adsorption Properties of Mesoporous Silica Functionalized with Aminopropyl Groups by the Co-Condensation and the Post Grafting Methods

The adsorptive potential has been evaluated for the aminopropyl functionalized mesoporous silica materials obtained by co-condensation and post grafting methods. Nitrogen sorption, small angle neutron and X-ray scattering (SANS and SAXS) demonstrated high surface area and well-ordered hexagonal pore structure suitable for applications as adsorbents of metals from waste waters. A comparison of Cr(VI) adsorption properties of the materials prepared by different functionalization methods has been performed. The obtained results demonstrated the adsorption capacity due to the affinity of the chromium ions to the amino groups, and showed that co-condensation of tetraethoxysilane (TEOS) and 3-aminopropyl triethoxysilane (APTES) resulted in higher metal sorption capacity of the materials compared to post-synthesis grafting of aminopropyl groups onto the mesoporous silica particles.

Grafting methods and use of fasteners in monoembryonic, polyembryonic, and multi-stemmed jaboticaba (Plinia jaboticaba (Vell) Berg) seedlings

Jaboticaba is a native fruit from Brazil, appreciated for its sweet taste and organoleptic characteristics. The propagation of this fruit species occurs exclusively through seeds, which present high germination rates, although with a long period of juvenility, a fact that favors advances in studies aiming at an early fruiting. This research aimed to study a more adequate and rapid grafting method for the jaboticaba cv. Sabará. The experimental design was in randomized blocks, with 12 treatments consisting of combinations using three types of seedlings (monoembryonic, polyembryonic, and multi-stemmed), two grafting methods (top cleft and splice grafting), and two fasteners (parafilm® tape and circular clip), with three replications consisting of 10 seedlings each. The analyzed characteristics were: monoembryony (%); polyembryony (%), multi-stemming (%), grafting success rate (%), survival (%), scion length (cm), scion diameter (mm), and rootstock diameter (mm). The percentage of monoembryony (46.4%) was higher than the success rates of polyembryony (36%), and multi-stemming (17.6%). The top cleft grafting method of monoembryonic seedlings fastened with parafilm® and circular clip, and polyembryonic seedlings fastened with circular clip were the more suitable for grafting in the jaboticaba cv. Sabará.