Confocal laser endomicroscopy of airways problems and prospects

We have described the principle and method of confocal laser endomicroscopy using the system Cellvizio lung probe Alveoflex (Mauna Kea Technologies, France) to study the distal lower respiratory tract in real time. Acynoscopy has been performed in 25 patients aged from 24 to 75 years with different lung pathology. The problems associated with using of this technology and its potential practical applications are discussed.

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G612(P) Enterovirus D68 real-time 2-step PCR: a useful diagnostic tool in lower respiratory tract infections in children?

10.1136/archdischild-2020-rcpch.526 ◽

2020 ◽

Author(s):

P Makiello ◽

A Vossen ◽

E Wessels

Keyword(s):

Respiratory Tract ◽

Real Time ◽

Diagnostic Tool ◽

Lower Respiratory Tract ◽

Respiratory Tract Infections ◽

Lower Respiratory Tract Infections ◽

Enterovirus D68 ◽

Tract Infections

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MP35-04 FIBEROPTIC CONFOCAL LASER ENDOMICROSCOPY OF SMALL RENAL MASSES: TOWARDS REAL TIME OPTICAL DIAGNOSTIC BIOPSY

The Journal of Urology ◽

10.1016/j.juro.2015.02.1105 ◽

2015 ◽

Vol 193 (4S) ◽

Cited By ~ 1

Author(s):

Li-Ming Su ◽

Robert Allan ◽

Kellie Ritari ◽

Patrick Tomeny ◽

Christopher Carter

Keyword(s):

Real Time ◽

Confocal Laser Endomicroscopy ◽

Confocal Laser ◽

Small Renal Masses ◽

Renal Masses ◽

Optical Diagnostic ◽

Diagnostic Biopsy

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Probe-based confocal laser endomicroscopy and Barrett's esophagus – just a scientific toy or significant improvement in diagnosis?

Digestive Diseases ◽

10.1159/000516257 ◽

2021 ◽

Author(s):

Luka Vranić ◽

Tin Nadarević ◽

Davor Štimac

Keyword(s):

Diagnostic Accuracy ◽

Real Time ◽

Barrett’S Esophagus ◽

Predictive Value ◽

Barrett's Esophagus ◽

High Reliability ◽

Confocal Laser Endomicroscopy ◽

Confocal Laser ◽

Imaging Method ◽

Increased Risk

Background: Barrett’s esophagus (BE) requires surveillance to identify potential neoplasia at early stage. Standard surveillance regimen includes random four-quadrant biopsies by Seattle protocol. Main limitations of random biopsies are high risk of sampling error, difficulties in histology interpretation, common inadequate classification of pathohistological changes, increased risk of bleeding and time necessary to acquire the final diagnosis. Probe-based confocal laser endomicroscopy (pCLE) has emerged as a potential tool with an aim to overcome these obvious limitations. Summary: pCLE represents real-time microscopic imaging method that offers evaluation of epithelial and subepithelial structures with 1000-fold magnification. In theory, pCLE has potential to eliminate the need for biopsy in BE patient. The main advantages would be real-time diagnosis and decision making, greater diagnostic accuracy and to evaluate larger area compared to random biopsies. Clinical pCLE studies in esophagus show high diagnostic accuracy and its high negative predictive value offers high reliability and confidence to exclude dysplastic and neoplastic lesions. However, it still cannot replace histopathology due to lower positive predictive value and sensitivity. Key messages: Despite promising results, its role in routine use in patients with Barrett’s esophagus remains questionable primarily due to lack of well-organized double-blind randomized trials.

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Real-time in vivo optical biopsy using confocal laser endomicroscopy to evaluate distal margin in situ and determine surgical procedure in low rectal cancer

Surgical Endoscopy ◽

10.1007/s00464-018-6519-z ◽

2018 ◽

Vol 33 (7) ◽

pp. 2332-2338

Author(s):

Zhangyuanzhu Liu ◽

Xiaobei Luo ◽

Wei Jiang ◽

Dexin Chen ◽

Weisheng Chen ◽

...

Keyword(s):

Rectal Cancer ◽

Real Time ◽

Surgical Procedure ◽

Low Rectal Cancer ◽

Optical Biopsy ◽

Confocal Laser Endomicroscopy ◽

Confocal Laser ◽

Distal Margin

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Confocal Laser Endomicroscopy in Neurosurgery: A New Technique with Much Potential

Minimally Invasive Surgery ◽

10.1155/2013/851819 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

David Breuskin ◽

Jana DiVincenzo ◽

Yoo-Jin Kim ◽

Steffi Urbschat ◽

Joachim Oertel

Keyword(s):

Real Time ◽

Histological Diagnosis ◽

New Technique ◽

Confocal Laser Endomicroscopy ◽

Confocal Laser ◽

Tissue Cell ◽

Resected Tissue ◽

Technical Innovations ◽

A New Technique ◽

Real Time Information

Technical innovations in brain tumour diagnostic and therapy have led to significant improvements of patient outcome and recurrence free interval. The use of technical devices such as surgical microscopes as well as neuronavigational systems have helped localising tumours as much as fluorescent agents, such as 5-aminolaevulinic acid, have helped visualizing pathologically altered tissue. Nonetheless, intraoperative instantaneous frozen sections and histological diagnosis remain the only method of gaining certainty of the nature of the resected tissue. This technique is time consuming and does not provide close-to-real-time information. In gastroenterology, confocal endoscopy closed the gap between tissue resection and histological examination, providing an almost real-time histological diagnosis. The potential of this technique using a confocal laser endoscope EndoMAG1 by Karl Storz Company was evaluated by our group on pig brains, tumour tissue cell cultures, and fresh human tumour specimen. Here, the authors report for the first time on the results of applying this new technique and provide first confocal endoscopic images of various brain and tumour structures. In all, the technique harbours a very promising potential to provide almost real-time intraoperative diagnosis, but further studies are needed to provide evidence for the technique’s potential.

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