scholarly journals CLINICAL AND LABORATORY FEATURES OF HHV-6 INFECTION IN IMMUNOCOMPROMISED CHILDREN FOLLOWED UP AT THE CHILDREN’S POLYCLINIC

2013 ◽  
Vol 18 (4) ◽  
pp. 35-39
Author(s):  
I. I Lvova ◽  
A. V Deryusheva ◽  
N. S. Legotina ◽  
E. V Sidor
Keyword(s):  
Herpes Virus ◽  
Intrauterine Infection ◽  
Medical Documentation ◽  
Pcr Assay ◽  
Diagnostic Measures ◽  
Qualitative Polymerase Chain Reaction ◽  
Laboratory Markers ◽  
Epidemiological Risk ◽  
Polymerase Chain ◽  
Herpès Virus

In a cohort of 100 immunocompromised children aged 3 months to 12 years in continuous screening of saliva and urine with a qualitative polymerase chain reaction (PCR) assay DNA herpes virus 4, 5, 6, type (CMV, EBV, HHV-6) were verified in 76% of cases. DNA herpes virus type 6 was detected significantly more often (56%). On the material of the primary medical documentation and data of clinical and laboratory examination in conditions of a children's polyclinic there was performed the analysis of clinical and epidemiological risk factors for intrauterine infection (IUI), and clinical and laboratory markers of secondary immune deficiency (SID). It was made a suggestion about predominant vertical transmission of HHV-6 during the antenatal period. Based on 100 percent of detection of opportunistic herpes viral DNA in children aged 1-3 years the tactics of "antigenic sparing" in young children with SID was justified. To optimize the diagnostic measures in respect of immunocompromised children in conditions of a children's polyclinic the performance of a comprehensive screening PCR testing for HSV infections, including HHV-6, as well as immunological and bacteriological control is proposed.

The effect of gold nanoparticles on the diagnostic polymerase chain reaction technique for equine herpes virus 1 (EHV-1)

RSC Advances ◽  
2016 ◽  
Vol 6 (60) ◽  
pp. 54898-54903 ◽  
Author(s):  
Dalia M. El-Husseini ◽  
Nashwa M. Helmy ◽  
Reham H. Tammam
Keyword(s):  
Polymerase Chain Reaction ◽  
Gold Nanoparticles ◽  
Herpes Virus ◽  
Polymerase Chain Reaction Technique ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Herpès Virus

We experimented the effect of 15 nm unmodified citrate coated GNPs on the key PCR reactants to see if these would enhance the overall outcomes of the reaction. Thus, the optimized GNPs-assisted PCR could be used for more efficient diagnosis of EHV-1.

scholarly journals Frequency of Herpes Virus (HSV) in Esophageal Muscle Samples among Patients with Achalasia under Heller Myotomy

2021 ◽  
Author(s):  
Tahmaseb Jouzdani ◽  
Amir Sadeghi ◽  
Hamed Tahmasbi ◽  
Ramin Shekouhi ◽  
Maryam Sohooli ◽  
...  
Keyword(s):  
Herpes Virus ◽  
Viral Agent ◽  
Heller’S Myotomy ◽  
History Of ◽  
Heller's Myotomy ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Herpès Virus ◽  
Esophageal Muscle ◽  
Hsv 1

Abstract Background Despite years of research, the etiology of achalasia not well understood. Scientists suppose a role for autoimmunity, in this disorder, and probable viral agent, such as herpes virus (HSV). The aim was to find out the frequency of HSV in esophageal muscle samples in patients with achalasia under Heller's myotomy. Methods In this study, 60 patients with achalasia, after fulfilling the consent form, were underwent Heller’s myotomy surgery. Biopsy samples prepared for polymerase chain reaction (PCR) method for HSV DNA detection. After DNA-extraction, replication performed using specific primers. Results The mean age was 40.62 ± 5.08 years. Thirty-nine patients (65%) were female and 21 (35%) were male. Thirty-eight (63.3%) had no history but the else (36.7%) had a positive history of HSV. HSV-1 was positive in three patients (5%). Two females and one male were HSV-positive. Conclusions HSV-1 frequency is not notable among Iranian patients with achalasia. We suggest exploring other viruses, in special that involving the pathogenesis of achalasia, with a larger sample size.

Role of Cytomegalovirus Replication in Alopecia Areata Pathogenesis

2000 ◽  
Vol 4 (2) ◽  
pp. 63-65 ◽  
Author(s):  
Annamaria Offidani ◽  
Paolo Amerio ◽  
Maria Luisa Bernardini ◽  
Claudio Feliciani ◽  
Guido Bossi
Keyword(s):  
Dna Sequences ◽  
Alopecia Areata ◽  
Herpes Virus ◽  
Cmv Infection ◽  
Tissue Samples ◽  
Triggering Factor ◽  
Polymerase Chain ◽  
Herpès Virus ◽  
Herpes Viridae

Background: Cytomegalovirus (CMV) infection has been correlated with various autoimmune disorders. Using molecular biology techniques, DNA sequences of CMV have been reported in paraffin sections of alopecia areata (AA) lesions. Reactivation of the CMV infection has been postulated as one of the pathogenic mechanisms in AA. Other studies, using different techniques however have demonstrated no correlation between CMV and AA. Objectives: This study was to clarify the role of CMV infection and to demonstrate the absence of replication of other autoimmune diseases-related herpes virus (EBV) in the pathogenesis of AA. Methods: After extraction of mRNA from tissue samples of a patient with active patchy AA, reverse transcriptase-polymerase chain reaction was carried out using primers specific for some viral members of the β-herpes viridae family (CMV, EBV, HSV). Results: No replication of the CMV or other β-herpes viridae has been detected in any of the samples collected. Conclusions: The results strongly support the hypothesis that CMV is not the triggering factor in AA, neither as a re-activator of the immune response nor as a trigger of the autoimmunity. No other herpes virus is implicated in the pathogenesis of this disease.

scholarly journals DETEKSI KOI HERPES VIRUS DENGAN METODE POLYMERASE CHAIN REACTION

2016 ◽  
Vol 14 (2) ◽  
pp. 115
Author(s):  
Dini Sahfitri Lubis ◽  
Diah Artati
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Virus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Herpès Virus

Polymerase chain reaction (PCR) merupakan salah satu metode deteksi cepat koi herpes vrus (KHV). Metode PCR ini sangat sensitif. Sensitivitas tersebut membuatnya dapat digunakan untuk melipatgandakan satu molekul DNA. Konsentrasi dan kualitas DNA dipengaruhi oleh keberhasilan pada saat melakukan ekstraksi DNA. Kegiatan percobaan ini bertujuan untuk mengetahui hasil deteksi virus KHV dengan metode PCR. Keberhasilan analisis PCR sangat dipengaruhi karakteristik DNA genom yang meliputi kemurnian, konsentrasi dan ukuran template. Virus KHV dideteksi menggunakan metode PCR sesuai dengan SNI 7547:2009. Hasil kegiatan deteksi virus KHV menggunakan PCR dari sampel kode I.230–I.286 dapat memvisualisasikan hasil PCR dengan positif KHV dengan ukuran fragmen DNA 290 bp dengan baik. Dapat dilihat dari 57 sampel yang telah diuji memiliki hasil yang berbeda-beda. Hasil uji yang diperoleh yaitu 32 sampel ikan positif terinfeksi KHV dan 25 sampel negatif KHV

scholarly journals Development of a Probe Based Real Time PCR Assay for Detection of Bovine Herpes Virus-1 in Semen and Other Clinical Samples

2012 ◽  
Vol 24 (1) ◽  
pp. 16-26
Author(s):  
Basavegowdanadoddi Marinaik Chandranaik ◽  
Doddamane Rathnamma ◽  
S. S. Patil ◽  
Ramesh C. Kovi ◽  
Jyotsana Dhawan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Herpes Virus ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Bovine Herpes Virus 1 ◽  
Herpès Virus

Parallel detection of five human herpes virus DNAs by a set of real-time polymerase chain reactions in a single run

2003 ◽  
Vol 26 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Markus Stöcher ◽  
Victoria Leb ◽  
Michael Bozic ◽  
Harald H Kessler ◽  
Gabriele Halwachs-Baumann ◽  
...  
Keyword(s):  
Real Time ◽  
Herpes Virus ◽  
Human Herpes ◽  
Chain Reactions ◽  
Parallel Detection ◽  
Polymerase Chain Reactions ◽  
Human Herpes Virus ◽  
Polymerase Chain ◽  
Herpès Virus

scholarly journals Diagnosis of Equine Herpes Virus 4 Infection using Polymerase Chain Reaction

2020 ◽  
Vol 9 (11) ◽  
pp. 887-890
Author(s):  
J. A. Vala ◽  
M. D. Patel ◽  
D. R. Patel ◽  
U. V. Ramani ◽  
I. H. Kalyani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Virus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Herpès Virus

scholarly journals Kaposi’s sarcoma-associated herpesvirus protein ORF75 among HIV-1 patients in Kenya

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Rodgers N. Demba ◽  
Sylviah M. Aradi ◽  
Matilu Mwau ◽  
Walter O. Mwanda
Keyword(s):  
Kaposi's Sarcoma ◽  
Herpes Virus ◽  
Deoxyribonucleic Acid ◽  
Kaposi’S Sarcoma ◽  
Nested Polymerase Chain Reaction ◽  
Reading Frame ◽  
Polymerase Chain ◽  
Herpès Virus ◽  
Hiv 1 ◽  
Histology Report

Background: Histology is used to identify Kaposi’s sarcoma (KS) in countries with low resources to fund healthcare costs. Approximately 95% of KS cases can be detected using a polymerase chain reaction.Objective: To determine the presence of the open reading frame 75 (ORF75) gene associated with Kaposi’s sarcoma herpes virus among HIV-1/AIDS patients and to describe morphological presentations of KS.Methods: This was a retrospective, descriptive study of archived tissue blocks collected from 2013 to 2016. Haematoxylin and eosin staining was used to identify KS. Deoxyribonucleic acid from archived tissue blocks was extracted and a nested polymerase chain reaction was used to detect the ORF75 gene.Results: All 81 cases in this study had been diagnosed as HIV-1 positive, of which 68 had hallmark features of KS in the histology report and 13 had features suggestive of KS (‘KS-like’). Microscopic identification of KS by haematoxylin and eosin staining was considered a significant indicator of KS herpes virus ORF75 gene positivity (p = 0.002). The ORF75 gene was detected in 60.5% (49/81) of tissue blocks; 27.2% were men (22/81) and 33.3% were women (27/81). The ORF75 gene was observed to be present in up to 15.4% (2/13) of the cases reported to have KS-like features.Conclusion: Following the initial diagnosis of KS by histology, the ORF75 gene was fur-ther detected from both cases that had hallmark features of KS as well as among cases with KS-like fea-tures.

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