"Conveyor machine" for collecting gastric juice

Free acid was found in the fasting gastric contents of each of 13 squirrel monkeys studied. A comparison of gastric contents from unrestrained and restrained monkeys indicated that restraint significantly increased the free acid concentration. Over a 24-hr period, there was a decrease in volume, free acid and total acid concentration, and pepsin value of the gastric juice. Histamine (0.1 mg/kg hr, subcutaneously) produced a significant increase in free acid and total acid concentration, while the volume and pepsin values were not changed. Methacholine, at cumulative doses of 1.0 and 2.0 mg/kg, subcutaneously, produced a significant decrease in free and total acid concentration while the volume was not altered. Atropine, in cumulative doses of 8, 16, and 48 mg/kg, intraperitoneally, produced a significant fall in volume of gastric juice collected. However, free acid concentration was reduced only at the highest dose and total acid concentration was not changed by any of the doses tested.

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The effect of gastric juice on the development of erosive changes in hard dental tissue

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh1408413s ◽

2014 ◽

Vol 142 (7-8) ◽

pp. 413-418 ◽

Cited By ~ 1

Author(s):

Ivana Stojsin ◽

Tatjana Brkanic ◽

Slavoljub Zivkovic

Keyword(s):

Gastric Juice ◽

Significant Degree ◽

Sem Analysis ◽

Dental Tissue ◽

Hard Dental Tissue ◽

Dental Hard Tissues ◽

Entry Points ◽

Gastric Contents ◽

Electron Microscopy Analysis ◽

The Moment

Introduction. Gastroesophageal reflux disease (GERD) is an esophageal disorder where the refluxed gastric contents enters first into the esophagus followed by the pharynx, oral cavity, larynx, airway and middle ear, causing a range of disorders and symptoms. Hydrochloric acid from the gastric contents is responsible for the demineralization of dental hard tissues and release of matrix metalloproteinase from the dentin. Objective. The aim of this study was to verify the SEM (scanning electron microscopy) analysis of the surface enamel, the enamel-dentin border and dentine after the exposure of intact teeth to filtrate of gastric contents obtained during routine endoscopy. Methods. Material used in the research was 10 extracted human impacted third molars. The coronal part of the tooth was divided into two parts, and then the two halves of teeth were exposed to the filtrate of gastric juice obtained during routine gastroscopy, which had been frozen until the moment of the experiment initiation. All samples of teeth were immersed in the filtrate of the content at a temperature of 20?C for 60 minutes. The prepared samples were observed by the SEM in the area of the enamel, the enamel-dentin border and in the area of dentin at different magnification. Results. The SEM analysis showed that both enamel and dentin had a significant demineralization of these tissues. Enamel surface resembled a demineralization similar to that of acid conditioning before the application of composite restorations. The degree of mineralization was more intense towards the enamel - dentin border, and at this area the enamel prisms were not fully recognizable. The dentin had a complete loss of peritubular dentin, the entry points of the dentin tubules were expanded and intertubular dentin demineralization was also registered. Conclusion. SEM analysis showed a significant degree of destruction of enamel and dentin. Significant changes in the surface structure of enamel and dentin were also registered. The filtrate of the gastric juice degrades enamel pellicle and the dentin organic component.

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THE SELF-REGULATION OF THE ACIDITY OF THE GASTRIC CONTENTS AND THE REAL ACIDITY OF THE GASTRIC JUICE

Quarterly Journal of Experimental Physiology ◽

10.1113/expphysiol.1914.sp000167 ◽

1914 ◽

Vol 8 (1) ◽

pp. 1-12 ◽

Cited By ~ 25

Author(s):

W. Boldyreff

Keyword(s):

Gastric Juice ◽

Self Regulation ◽

The Self ◽

The Real ◽

Gastric Contents

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JUVENILE PERNICIOUS ANEMIA

PEDIATRICS ◽

10.1542/peds.8.1.88 ◽

1951 ◽

Vol 8 (1) ◽

pp. 88-106

Author(s):

EDWARD H. REISNER ◽

JAMES A. WOLFF ◽

R. JAMES MCKAY ◽

EUGENIA F. DOYLE

Keyword(s):

Vitamin B12 ◽

Pernicious Anemia ◽

Gastric Juice ◽

Liver Extract ◽

Macrocytic Anemia ◽

Intrinsic Factor ◽

The Other ◽

Free Hydrochloric Acid ◽

Laboratory Findings ◽

Gastric Contents

Histories have been presented of two pairs of sibling children with recurrent macrocytic anemia with megaloblastic bone marrow responding specifically to liver extract and vitamin B12. The clinical features and laboratory findings were similar to those of adult pernicious anemia in every respect except for the inconstant presence of histamine refractory achlorhydria. All the patients responded to vitamin B12 given parenterally, but in three to whom it was given by mouth no response was obtained. Two of these subsequently responded to oral B12 when it was accompanied by normal gastric juice. The other two developed histamine refractory achlorhydria while in hematologic remission. This is interpreted as proof that the anemia was due to deficient intrinsic factor in the gastric juice. Three patients showed evidence of disease of the spinal cord, in two of whom the symptoms were severe. These symptoms were compatible with a diagnosis of dorsolateral sclerosis, and improved with antipernicious anemia therapy. In one of these patients, treated with folic acid, the neurologic lesions were aggravated severely. Pernicious anemia due to the absence of gastric juice intrinsic factor occurs in children. The presence of free hydrochloric acid in the gastric contents does not preclude the possibility of the disease.

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Fractional study of gastric contents with a thin probe

Kazan medical journal ◽

10.17816/kazmj50375 ◽

1926 ◽

Vol 22 (1) ◽

pp. 102-103

Author(s):

L. Rakhlin

Keyword(s):

Gastric Digestion ◽

Gastric Contents

Fractional study of gastric contents with a thin probe, according to Vandorf'y (Klin. Woch, 1925, No. 29) makes it possible to trace the entire course of gastric digestion.

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A new principle in surgical treatment of peptic ulcer. Prof. Bogoraz (New Khir. Arkh., vol. 3, b. 2)

Kazan medical journal ◽

10.17816/kazmj80136 ◽

1923 ◽

Vol 19 (6) ◽

pp. 98-98

Author(s):

V. Bogolyubov

Keyword(s):

Peptic Ulcer ◽

Gastric Ulcer ◽

Surgical Treatment ◽

Gastric Juice ◽

Gastric Resection ◽

Relative Increase ◽

Duodenal Juice ◽

Gastric Digestion ◽

The Absolute

Of the peculiarities of gastric digestion in peptic ulcer the absolute or relative increase in acidity of gastric juice is in the foreground. Gastric ulcer surgeries-gastroenterostomy (alone or with disconnection of the pylorus) and gastric resection-achieve their purpose by facilitating the discharge of duodenal juice into the stomach and a more rapid emptying of the stomach of its contents.

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Gastric digestion of α-lactalbumin in adult human subjects using capsule endoscopy and nasogastric tube sampling

British Journal Of Nutrition ◽

10.1017/s0007114514001196 ◽

2014 ◽

Vol 112 (4) ◽

pp. 638-646 ◽

Cited By ~ 14

Author(s):

Louise M. Sullivan ◽

Joseph J. Kehoe ◽

Lillian Barry ◽

Martin J. M. Buckley ◽

Fergus Shanahan ◽

...

Keyword(s):

Capsule Endoscopy ◽

Protein Unfolding ◽

Structural Changes ◽

Human Subjects ◽

Native Protein ◽

Gastric Digestion ◽

Nasogastric Tubes ◽

Subsequent Decrease ◽

Gastric Contents

In the present study, structural changes in the milk protein α-lactalbumin (α-LA) and its proteolysis were investigated for the potential formation of protein–fatty acid complexes during in vivo gastric digestion. Capsule endoscopy allowed visualisation of the digestion of the test drinks, with nasogastric tubes allowing sampling of the gastric contents. A total of ten healthy volunteers had nasogastric tubes inserted into the stomach and ingested test drinks containing 50 g/l of sucrose and 25 g/l of α-LA with and without 4 g/l of oleic acid (OA). The samples of gastric contents were collected for analysis at 3 min intervals. The results revealed a rapid decrease in the pH of the stomach of the subjects. The fasting pH of 2·31 (sd 1·19) increased to a pH maxima of pH 6·54 (sd 0·29) after ingestion, with a subsequent decrease to pH 2·22 (sd 1·91) after 21 min (n 8). Fluorescence spectroscopy and Fourier transform IR spectroscopy revealed partial protein unfolding, coinciding with the decrease in pH below the isoelectric point of α-LA. The activity of pepsin in the fasting state was found to be 39 (sd 12) units/ml of gastric juice. Rapid digestion of the protein occurred: after 15 min, no native protein was detected using SDS–PAGE; HPLC revealed the presence of small amounts of native protein after 24 min of gastric digestion. Mirocam® capsule endoscopy imaging and video clips (see the online supplementary material) revealed that gastric peristalsis resulted in a heterogeneous mixture during gastric digestion. Unfolding of α-LA was observed during gastric transit; however, there was no evidence of a cytotoxic complex being formed between α-LA and OA.

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