scholarly journals Zebrafish: Recomendações de Manutenção em Laboratório

UNICIÊNCIAS ◽  
2021 ◽  
Vol 24 (2) ◽  
pp. 141-145
Author(s):  
Carla Letícia Gediel Rivero-Wendt ◽  
Rosemary Matias ◽  
Giovana Coutinho Zulin do Nascimento ◽  
Silvia Cristina Heredia-Vieira ◽  
Magno Rafael Miranda Santos ◽  
...  

O zebrafish ou paulistinha (Danio rerio) são cada vez mais utilizados em pesquisas científicas incluindo a pesquisa básica e aplicada nas áreas de biomedicina, toxicologia, ciências ambientais e aquicultura. No Brasil, os biotérios automatizados são escassos, têm custo oneroso e para fazer uso do modelo animal são necessárias adaptações para criação e manutenção da espécie. Deve existir a compreensão de que as adaptações devem respeitar os padrões de qualidade, implicando na diminuição de mortalidade e doenças nos aquários de manutenção. O objetivo desta revisão de literatura é apresentar as recomendações de protocolo de manutenção do zebrafish, em condições de laboratório, sem o uso de biotério automatizado. Apesar da gama de informações e diferentes métodos de manutenção encontrados para a espécie, fica claro que em condições de laboratório se deve manter o animal nas melhores condições, possibilitando qualidade no desenvolvimento e na reprodução para realizar experimentos com confiabilidade de execução.   Palavras-chave: Desenvolvimento. Ambiente e Sustentabilidade. Danio rerio. Biotério.   Abstract The use of Zebrafish or paulistinha (Danio rerio) has increased considerably in basic and applied scientific research as biomedicine, toxicology, environmental sciences and aquaculture. In Brazil, automated experimental animal facilities are scarce and costly. To make use of this animal model, adaptations are required for creation and maintenance. These changes must respect quality standards, providing mortality and disease reduction in aquariums maintenance. The literature review aim is to synthesize recommendations for the zebrafish maintenance protocol in laboratory conditions without the automated experimental animal facility. Despite the information range and different maintenance methods found for the species, it is clear that under laboratory conditions, the animal must be kept in the best conditions, enabling development and reproduction quality to be used in experiments with reliable execution. Keywords: Development. Environment and Sustainability. Danio rerio. Experimental Animal Facility.

2018 ◽  
Vol 35 (1) ◽  
pp. 28-45
Author(s):  
Eman Salem ◽  
Hamdy Hassan ◽  
Ibrahim Badr ◽  
Ethar Mohamed ◽  
Bakinam Mohamed ◽  
...  

Author(s):  
Nirit Putievsky Pilosof ◽  
Yasha Jacob Grobman

Objective The study examines the integration of the Evidence-based Design (EBD) approach in healthcare architecture education in the context of an academic design studio. Background Previous research addressed the gap between scientific research and architectural practice and the lack of research on the use of the EBD approach in architectural education. Methods The research examines an undergraduate architectural studio to design a Maggie’s Centre for cancer care in Israel and evaluates the impact of the EBD approach on the design process and design outcomes. The research investigates the impact of the integration of three predesign tasks: (1) literature review of healing architecture research, (2) analysis and comparison of existing Maggie’s Centres, and (3) analysis of the context of the design project. Results The literature review of scientific research supported the conceptual design and development of the projects. The analysis of existing Maggie’s centers, which demonstrated the interpretation of the evidence by different architects, developed the students’ ability to evaluate EBD in practice critically, and the study of the projects’ local context led the students to define the relevance of the evidence to support their vision for the project. Conclusions The research demonstrates the advantages of practicing EBD at an early stage in healthcare architectural education to enhance awareness of the impact of architectural design on the users’ health and well-being and the potential to support creativity and innovative design. More studies in design studios are needed to assess the full impact of integrating EBD in architectural education.


Seizure ◽  
2006 ◽  
Vol 15 (5) ◽  
pp. 275-287 ◽  
Author(s):  
O. Mameli ◽  
M.A. Caria ◽  
A. Pintus ◽  
G. Padua ◽  
S. Mameli

2006 ◽  
Vol 47 (2) ◽  
pp. 729 ◽  
Author(s):  
Diego G. Espinosa-Heidmann ◽  
Ivan J. Suner ◽  
Paola Catanuto ◽  
Eleut P. Hernandez ◽  
Maria E. Marin-Castano ◽  
...  

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e102993 ◽  
Author(s):  
Ivan Dimauro ◽  
Loredana Grasso ◽  
Simona Fittipaldi ◽  
Cristina Fantini ◽  
Neri Mercatelli ◽  
...  

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