scholarly journals Effect of Saliva/Blood Contamination on Enamel Bond Strength

2021 ◽  
Vol 23 (4) ◽  
pp. 277-281
Author(s):  
Rodrigo Galo ◽  
Isabela Maluta ◽  
Marta Maria Martins Giamatei Contente ◽  
Carolina Paes Torres ◽  
Maria Cristina Borsatto

AbstractIn the routine clinical situation, the contamination by blood and/or saliva in restorative procedures can be happen in non-cooperation of the patient in dental office. The aim of the study was to assess in vitro shear bond strength of a resin sealant associated with two types of adhesives contaminated with saliva and blood. Healthy human molars were used and the specimens and the crowns were sectioned in the bucco-lingual direction, thus obtaining two segments of similar proportions (mesial and distal), totaling 60 surfaces, and the surfaces were randomly divided into 4 groups (n = 15). Group I (control) received no type of contamination and the sealant was applied. In group II, the surfaces were contaminated with 10 μl of saliva/blood and the sealant was applied. In group III, the surfaces were contaminated with 10 μl of saliva/blood and the Single Bond total-etch adhesive system was applied followed by application of sealant. In group IV, the surfaces were contaminated with 10 μl of saliva/blood and the Prime & Bond NT total-etch adhesive system was applied followed by the application of sealant. Samples were tested in the universal testing machine and the analysis of shear bond strength was performed. A difference between Group I (12.61MPa) and the other groups was found; Group II (2. 28MPa) was different than Groups III (7.07MPa) and IV (7.79MPa), but Groups III and IV were similar. The application of an adhesive system when there is contamination with saliva/blood is required prior to application of pit and fissure sealants. Keywords: Pit and Fissure Sealants. Biological Contamination. Shear Strength. ResumoNa situação clínica de rotina, a contaminação por sangue e/ou saliva em procedimentos restauradores pode ocorrer em pacientes que não colaboram no consultório odontológico. O objetivo do estudo foi avaliar a resistência ao cisalhamento in vitro de um selante de resina associado a dois tipos de adesivos contaminados com saliva e sangue. Foram utilizados molares humanos saudáveis e os espécimes e as coroas foram seccionados na direção bucal-lingual, obtendo assim dois segmentos de proporções semelhantes (mesial e distal), totalizando 60 superfícies, e as superfícies foram divididas aleatoriamente em 4 grupos (n = 15). O Grupo I (controle) não recebeu nenhum tipo de contaminação e o selante foi aplicado. No grupo II, as superfícies foram contaminadas com 10 μl de saliva / sangue e o selante foi aplicado. No grupo III, as superfícies foram contaminadas com 10 μl de saliva / sangue e o sistema adesivo Single-Bond foi aplicado seguindo a aplicação de selante. No grupo IV, as superfícies foram contaminadas com 10 μl de saliva / sangue e o sistema adesivo de ataque total Prime & Bond NT foi aplicado seguido da aplicação de vedante. As amostras foram testadas na máquina de ensaio universal e a análise da resistência à ligação ao cisalhamento foi realizada. Uma diferença entre o Grupo I (12,61MPa) e os outros grupos foi encontrada; O Grupo II (2,28 MPa) foi diferente dos Grupos III (7,07MPa) e IV (7,79 MPa), mas os Grupos III e IV foram semelhantes. A aplicação de um sistema adesivo quando existe contaminação com saliva / sangue é necessária antes da aplicação de selantes de fissura e fissura.. Palavras-chave: Selantes de Fossas e Fissuras. Contaminação Biológica. Resistência ao Cisalhamento.

2012 ◽  
Vol 17 (4) ◽  
pp. 1-7 ◽  
Author(s):  
Roberto Hideo Shimizu ◽  
Karlos Giovani Grando ◽  
Isabela Almeida Shimizu ◽  
Augusto Ricardo Andriguetto ◽  
Ana Cláudia Moreira Melo ◽  
...  

OBJECTIVE: This in vitro study was designed to evaluate the shear bond strength (SBS) of orthodontic metal brackets bonded by direct and indirect techniques. METHODS: Thirty healthy human maxillary premolar teeth were used. The teeth were divided into three groups of 10 teeth each: Group I - indirect bonding with SondhiTM Rapid-Set system (3M/Unitek), Group II - indirect bonding with TransbondTM XT adhesive system (3M/Unitek) and Group III - direct bonding with TransbondTM XT adhesive system (3M/Unitek). After bonding and obtaining the specimens for the study, the specimens were subjected to SBS testing in a universal testing machine (Emic, model DL-500). The Kolmogorov-Smirnov test was applied to ascertain that the data had a normal distribution and the Bartlett test to check whether there was homogeneity of variance. One-factor analysis of variance was performed and, subsequently, Tukey's test for paired means. A 5% significance level was adopted. RESULTS: The results of Group I were 67.6 (N) and 5.9 (MPa); Group II, 68.9 (N) and 6.1 (MPa) and Group III (control), 92.5 (N) and 8.1 (MPa). CONCLUSION: It can therefore be concluded that the means for Group III were significantly higher compared with Groups I and II in both Newton (N) and Megapascal (MPa) values. The means attained by the indirect bonding technique used in Groups I and II, however, exhibited no statistically significant differences.


Author(s):  
Dr. Sundaresan Balagopal ◽  
Dr. Bahavathi Ananthan Hemasathya ◽  
Dr. Ramasamy Saravanan Saravanan Gayathri ◽  
Dr. M. Anisha Anisha Sebatni ◽  
Dr. Vasudevan Navabharathy

Aim: The aim of the study was to evaluate the shear bond strength of composite to dentin with 2 different dentin bonding agents after dry dentin is rewetted with a desensitizer. Materials and Methods: Sixty human mandibular molars were taken and the occlusal surface was ground to expose flat dentinal surface.  The samples were divided into two major groups Group I and Group II based on the bonding agent used. In Group I Gluma Bond5 was used and in Group II Adper Single Bond 2 was used.  Each major group is subdivided into three subgroups a, b and c with ten samples each.  Sub Group ‘a’ is moist dentin group. Sub Group ‘b’ is dry dentin group, Sub Group ‘c’ is rewetted dentin group. The desensitizer used is GLUMA Desensitizer. All these samples were thermocycled and the shear bond test was performed using Instron Universal Testing Machine. Results: Dry dentin rewetted with GLUMA Desensitizer followed by GLUMA Bond5 showed highest shear bond strength than that of other subgroups. The data was analysed using ANOVA and Tukeys honestly significant test. Conclusion: Desensitizer used as a rewetting agent increases the shear bond strength of composite on dry dentin. Keywords: Glutaraldehyde, rewetting.


Coatings ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 273
Author(s):  
Abdulmajeed Ali Asiri ◽  
Rawaiz Khan ◽  
Sultan Saeed Alzahrani ◽  
Sajjad Haider ◽  
Salah Ud-Din Khan ◽  
...  

This study aimed to measure and compare the shear bond strength (SBS) of a self-adhering flowable resin composite to dentin with a conventional flowable composite, and evaluate the various modes of failure. Thirty human premolar teeth (n = 30) were randomly allocated to two groups (Group I: Vertise™ Flow; Group II: Filtek Ultimate + Single Bond Universal) of 15 specimens each, and SBS was measured for specimens from each group by applying a shear load using a universal testing machine (UTM). Modes of failure were observed with an optical microscope. An independent-samples T-test was performed to test Levene’s assumption of homogeneity of variance across both groups, with the critical value set at 0.05. The results revealed, that the self-adhering flowable composite (Group I: Vertise™ Flow) resulted in a lower shear bond strength compared with the conventional adhesive system composite (Group II: Filtek Ultimate + Single Bond Universal). Moreover, group I predominantly exhibited non-cohesive failure, which reflects poor bonding to dentin. On the other hand, Group II showed mixed failure for most of the samples, which demonstrated strong adhesive bonding. Therefore, it could be concluded that the self-adhering flowable resin composite (Group I) results in lower SBS to dentin compared with a conventional adhesive system.


2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Zohreh Moradi ◽  
Farnoosh Akbari ◽  
Sara Valizadeh

Aim. This study aimed to assess shear bond strength (SBS) of resin cement to zirconia ceramic with different surface treatments by using Single Bond Universal. Methods. In this in vitro study, 50 zirconia discs (2 × 6 mm) were divided into 5 groups of (I) sandblasting with silica-coated alumina (CoJet)  + silane + Single Bond 2, (II) sandblasting with CoJet + Single Bond Universal, (III) sandblasting with alumina + Single Bond Universal, (IV) sandblasting with alumina + Z-Prime Plus, and (V) Single Bond Universal with no surface treatment. Resin cement was applied in plastic tubes (3 × 5 mm2), and after 10,000 thermal cycles, the SBS was measured by a universal testing machine. The mode of failure was determined under a stereomicroscope at × 40 magnification. Data were analyzed using one-way ANOVA. Results. The maximum (6.56 ± 4.29 MPa) and minimum (1.94 ± 1.96 MPa) SBS values were noted in groups III and I, respectively. Group III had the highest frequency of mixed failure (60%). Group V had the maximum frequency of adhesive failure (100%). Conclusion. Single Bond Universal + sandblasting with alumina or silica-coated alumina particles is an acceptable method to provide a strong SBS between resin cement and zirconia.


2019 ◽  
Vol 7 (13) ◽  
pp. 2162-2166 ◽  
Author(s):  
Rasha M. Abdelraouf ◽  
Manar Mohammed ◽  
Fatma Abdelgawad

AIM: This study aimed to assess the shear bond strength of a self-adhering flowable resin composite versus a total-etch one to different surfaces of permanent-molars. MATERIAL AND METHODS: Thirty-six sound human permanent molars were used. The teeth were embedded in acrylic blocks, such that their buccal surfaces were shown. The teeth were divided into three groups: Group I: Uncut-Enamel, Group II: Cut-enamel-surfaces with minimal-grinding and Group III: dentin-surfaces. Half of the teeth in each group were used for bonding to a self-adhering flowable resin-composite (Dyad-flow, Kerr, USA). While the other half of each group was bonded to a total-etch flowable resin-composite (Filtek™Z350-XT,3M-ESPE, USA) which necessitate etching and bonding. Teflon-mold was used for constructing resin composite cylinders (3 × 3 mm) over the buccal surfaces. The Dyad-flow was applied in the central hole of the mould placed upon tooth-surface, and then light-cured for 20 seconds. The Filtek-Z350-XT was applied similarly after etching and bonding steps. The teeth were stored in 37°C distilled water for 24 hours. The strength was measured using a universal testing machine and statistically analysed. Modes of failure were studied using digital-microscope. RESULTS: Mean values of shear bond strength for the Dyad and Filtek-Z350-XT in the uncut-enamel were 3.5 and 24.6MPa respectively, while that for cut-enamel were 4.5 and 12.7MPa respectively (Both highly statistically significant P ≤ 0.01) and in dentin were 4.3 and 6.7MPa respectively (Statistically significant P ≤ 0.05). The failure mode for Dyad was mainly adhesive (un-cut or cut-enamel 83.3% adhesive and 16.7% mixed, while in dentin 100% adhesive). While the modes of failure for Filtek-Z350-XT in enamel, either cut or un-cut, were 50% cohesive and 50% mixed, whereas in dentin 100% adhesive. CONCLUSION: Bonding of self-etch ″Dyad-flow″ flowable resin-composite was lower than the total-etch one in enamel and dentin. Thus further material improvement may be required.


2014 ◽  
Vol 13 (1) ◽  
pp. 7
Author(s):  
Dewi Puspitasari ◽  
Andi Soufyan ◽  
Ellyza Herda

Composite resin is a widely used aesthetic restoration. The restoration can fail due to secondary caries. Chlorhexidinegluconate 2% is used as a cavity disinfectant to eliminate microorganisms on the prepared cavity and to prevent thesecondary caries. The purpose of this study was to analyze the effect of chlorhexidine gluconate 2% to the bondstrength of composite resin with self etch system adhesive on dentine. Sixteen specimens of buccal dentine of premolarscrown are divided into 2 different groups. Group I: Clearfil SE Bond self-etch primer was applied for 20 seconds,Clearfil SE Bond bonding was applied for 5 seconds and polymerized for 10 seconds. Composite resin was constructedincrementally and polymerized for 20 seconds. Group II: prior to self etch primer application as in group I,chlorhexidine gluconate 2% was applied for 15 seconds. Shear bond strength was tested using Testing machine andanalyzed with unpaired T test. The highest shear bond strength was obtained by applying chlorhexidine gluconate 2%.The study concludes that chlorhexidine gluconate 2% application to dentine did not affect significantly to the bondstrength composite resin using self etch adhesive systems.


2018 ◽  
Vol 19 (6) ◽  
pp. 642-646
Author(s):  
BS Kavya Rani ◽  
Surya Viswambharapanicker ◽  
Shamil Mattumathody ◽  
Anagha Muralidharan ◽  
Abdul Rasheed Naseema Dinsha ◽  
...  

2021 ◽  
Vol 10 (40) ◽  
pp. 33-37
Author(s):  
Joyce de Figueiredo Meira Barbosa ◽  
Lara Pepita de Souza Oliveira ◽  
Marcelo Nascimento Bruce ◽  
Jonas Alves Oliveira ◽  
Ligia Regina Mota Vasconcelos ◽  
...  

Acrylic denture teeth may suffer fracture or wear requiring the need for repair. This study aimed to evaluate the shear bond strength between acrylic resin artificial teeth restored with composite resin (with two different surface treatments) and acrylic resin (AR), simulating repair with these materials. Thirty artificial incisors were included by the palatal side in a circular microwave-polymerized AR base and polished on their buccal side with 120 and 320 grit sandpaper in a metallographic polisher and, then, divided into three groups: (I) restoration with self-cured AR; (II) conventional restoration with Z-100 composite resin with application of acid etching and Single Bond-3M adhesive; and (III) restoration with Z-100 composite resin with submersion for 30 seconds in acetone and application of Single Bond-3M adhesive. Shear bond strength tests were performed in a Universal Testing Machine (Instron). The results were submitted to ANOVA and Tukey (p<0.05) tests, in which Group I (33.26MPa ±10.76) and Group III (22.24MPa ±13.13) showed no statistically significant difference, but both were superior to Group II (10.31MPa ±5.62), which showed a lower value of shear bond strength. It can be concluded that composite resin restoration with acetone pretreatment can be a viable alternative for repair.


Author(s):  
Zahra Khamverdi ◽  
Naemeh Karimian ◽  
Maryam Farhadian ◽  
Hamed Gheitouli

Objectives: Bond strength of composite restorations plays an important role in their success. This in vitro study evaluated the effect of a hemostatic agent on shear bond strength of universal adhesives. Materials and Methods: Thirty-six extracted human molars were used in this study. Buccal and lingual surfaces were reduced to obtain flat dentin surfaces and were ground with a silicone paper. The samples were randomly divided into three groups (n=12) based on the application of hemostatic agent: group 1: no contamination (control), group 2: aluminum chloride application, and group 3: ferric sulfate application. Each group was then divided into two subgroups (n=6) for using G-Premio and Single Bond Universal. Resin cylinders (Filtek Z550) were bonded to dentin surfaces according to the manufacturer’s instructions. After 1000 thermal cycles, shear load was applied to the specimens using a universal testing machine at a crosshead speed of 1 mm/min until failure. Data were statistically analyzed using two-way ANOVA and Tukey’s post-hoc test (α=0.05). Results: There were statistically significant differences in shear bond strength of the three main groups for both G-Premio and Single Bond Universal (P<0.05). When the adhesive systems were compared with each other, G-Premio showed higher shear bond strength than Single Bond Universal (P<0.05). Conclusion: Contamination with hemostatic agents had an adverse effect on the shear bond strength of universal adhesives. Moreover, G-Premio yielded a higher bond strength than Single Bond Universal.


2019 ◽  
Vol 26 (08) ◽  
pp. 1377-1681
Author(s):  
Kashif Haroon ◽  
Uzma Ijaz ◽  
Saad Haroon ◽  
Taimoor Khan ◽  
Raheela Yasmin ◽  
...  

The revolution in orthodontics has been due to replacement of cemented bands by orthodontic brackets. Acid etching is required to bond a bracket on tooth’s surface. Traditional three step acid etch procedure consisted of etching, sealing/priming and bonding. The self etch system (etchant and primer together) can be used without the need for surface preparation, saving chair side time and minimizing contamination. Objectives: To compare the shear bond strength (SBS) of orthodontic bracket bonded with Conventional etching primer (Transbond XT, 3M Unitek) with self etch primer (Adper Prompt L-Pop, 3M ESPE). Study Design: Prospective study Setting: Orthodontic Clinic of Ihsan Mumtaz Hospital, Lahore and PCSIR (Lahore). Period: 6 months from June 2018 to December 2018. Material and Methods: 60 human maxillary premolars were randomly divided into 2 groups of 30 each. Group-I was bonded with conventional etching primer + composite and Group-II was bonded with self etch primer + composite. Shear bond strength was measured through Ionstron force testing machine. Statistical Analysis: For each variable, shear bond strength, arithmetic mean, standard deviation, minimum and maximum values were calculated. t-test was used to compare the shear bond strength (SBS) of the two groups and P value less than and equal to 0.05 was considered significant. Results: The mean shear bond strength in group 1 and group II was10.5MPa and 7.36MPa respectively. Conclusion: The shear bond strength of Conventional etching primer + adhesive was significantly higher than the shear bond strength of self etch primer +adhesive group.


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