scholarly journals Molecular Identification of Human Sporotrichosis in Korea

2019 ◽  
pp. 45-51
Author(s):  
Hye Ri Kim ◽  
Dong Hoon Shin ◽  
Jee-Bum Lee ◽  
Jong Soo Choi
Keyword(s):  
Northeast China ◽  
Sporothrix Schenckii ◽  
Sensu Stricto ◽  
Its Sequences ◽  
Predominant Species ◽  
Molecular Studies ◽  
Deep Mycosis ◽  
Causative Species ◽  
Group B ◽  
Private Collections

Background: Sporotrichosis is a common deep mycosis caused by the Sporothrix schenckii complex. Until 2016, no molecular studies had been conducted on these fungi, and all the included strains were reported as S. schenckii. However, investigations conducted in northeast China, Japan, and India revealed that S. globosa was the most prevalent Sporothrix species, whereas S. schenckii sensu stricto was reported very rarely. Objective: To investigate the accurate prevalent causative species of sporotrichosis among strains reported as S.schenckii in Korea. Methods: We isolated strains of Sporothrix spp. Prevalent in Korea from fungus collection centers or private collections and reviewed the available literature on molecular studies of strains from this region. We found five S. schenckii (1998-2016) and three S. globosa (2016-2018) strains. Ribosomal DNA internal transcribed spacer (ITS) sequences of these strains were compared with those of the S. schenckii complex strains. Results: The ribosomal ITS sequences of the eight strains were 100% identical with that of S. globose. No S. schenckii sensu stricto was found. In addition, a study on the molecular analysis of Korean S. schenckii published by Ishizaki et al. (2004) demonstrated that the eight strains were of the mitochondrial subtype group B (S. globosa). Thus, all the 16 strains examined within the Korean S. schenckii complex were determined to be S. globosa. Conclusion: In summary, S. globosa is the causative species within the tested Korean sporotrichosis cases reported between 1998 and 2018. Based on our analyses, S. globosa, and not S. schenckii, may be the predominant species in Korea.

Identification of multiple species and subpopulations among Australian clinical Sporothrix isolates using whole genome sequencing

2018 ◽  
Vol 57 (7) ◽  
pp. 905-908 ◽  
Author(s):  
David New ◽  
Alicia G Beukers ◽  
Sarah E Kidd ◽  
Adam J Merritt ◽  
Kerry Weeks ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Sporothrix Schenckii ◽  
Sensu Stricto ◽  
Northern Territory ◽  
Whole Genome ◽  
Phylogenetic Resolution ◽  
Genetic Clustering ◽  
Multiple Species

AbstractWhole genome sequencing (WGS) was used to demonstrate the wide genetic variability within Sporothrix schenckii sensu lato and establish that there are two main species of Sporothrix within Australian clinical isolates—S. schenckii sensu stricto and Sporothrix globosa. We also demonstrated southwest Western Australia contained genetically similar S. schenckii ss strains that are distinct from strains isolated in the eastern and northern states of Australia. Some genetic clustering by region was also noted for northern NSW, Queensland, and Northern Territory. Phylogenetic analysis of WGS data provided greater phylogenetic resolution compared to analysis of the calmodulin gene alone.

On the disintegration of Molluginaceae: a new genus and family (Kewa, Kewaceae) segregated from Hypertelis, and placement of Macarthuria in Macarthuriaceae

Phytotaxa ◽  
2014 ◽  
Vol 181 (4) ◽  
pp. 238 ◽  
Author(s):  
Maarten J.M. Christenhusz ◽  
Samuel F. Brockington ◽  
Pascal-Antoine Christin ◽  
Rowan F. Sage
Keyword(s):  
Type Species ◽  
New Genus ◽  
Sensu Stricto ◽  
New Family ◽  
Family Names ◽  
Molecular Studies ◽  
Traditional Sense

Molecular studies have shown that Molluginaceae in the traditional sense is polyphyletic. Several genera have already been separated into various families (e.g. Caryophyllaceae, Limeaceae, Lophiocarpaceae, Microteaceae), but recent studies have shown that Macarthuria and Hypertelis also make Molluginaceae polyphyletic if they remain to be included in this family. Hypertelis is biphyletic, with its type species found to belong to Molluginaceae sensu stricto, but the remainder of the genus is to be placed elsewhere. Therefore a new genus, Kewa, is proposed for the rest of Hypertelis, and two new family names are coined: Kewaceae and Macarthuriaceae, which are here morphologically characterized.

Cortinarius khinganensis (Agaricales), a new species of section Illumini from Northeast China

Phytotaxa ◽  
2021 ◽  
Vol 500 (1) ◽  
pp. 1-10
Author(s):  
MENG-LE XIE ◽  
TIE-ZHENG WEI ◽  
BÁLINT DIMA ◽  
YONG-PING FU ◽  
RUI-QING JI ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Relationships ◽  
Northeast China ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Sensu Stricto ◽  
Similar Species ◽  
Molecular Phylogenetic ◽  
A New Species

This study presents one telamonioid species new to science based on morphological characteristics and molecular phylogenetic analyses. Cortinarius khinganensis was collected from the Greater Khingan Mountains, Northeast China and it is characterized by hygrophanous, vivid brownish red and striate pileus, white universal veil, and subglobose spores. According to phylogenetic analyses results, C. khinganensis belongs to the section Illumini, which is a lineage distantly related from subgenus Telamonia sensu stricto. Detailed descriptions of the new species and the comparisons with morphologically similar species are provided. The phylogenetic relationships within the section Illumini are also discussed.

Geocerthia, a new genus of terrestrial ovenbird (Aves: Passeriformes: Furnariidae)

Zootaxa ◽  
2009 ◽  
Vol 2213 (1) ◽  
pp. 64-68 ◽  
Author(s):  
R. TERRY CHESSER ◽  
SANTIAGO CLARAMUNT ◽  
ELIZABETH DERRYBERRY ◽  
ROBB T. BRUMFIELD
Keyword(s):  
New Genus ◽  
Sensu Stricto ◽  
Molecular Studies ◽  
Form Part

The avian genus Upucerthia was until recently considered to consist of nine species (Sibley and Monroe 1990, Dickinson 2003, Remsen 2003) of mainly terrestrial ovenbirds, commonly known as earthcreepers. Recent molecular studies of the genus (Chesser et al. 2007, Fjeldså et al. 2007) indicated that Upucerthia, as traditionally constituted, was highly polyphyletic, its nine species apparently belonging to four distinct lineages. Four species of Upucerthia – albigula, jelskii, validirostris, and the name-bearing species dumetaria – formed a well-supported clade sister to Cinclodes (Chesser et al. 2007, Fjeldså et al. 2007). Of the five other species of Upucerthia, U. andaecola and U. ruficaudus formed a clade with Eremobius phoenicurus and Chilia melanura, all of which were subsequently transferred to the genus Ochetorhynchus (Chesser et al. 2007, Fjeldså et al. 2007). Upucerthia harterti and U. certhioides formed a distinct clade and were placed in the newly erected genus Tarphonomus (Chesser and Brumfield 2007). The position of the final species, U. serrana, was not well resolved, although it appeared to form part of a clade including the genera Cinclodes and Upucerthia sensu stricto (Chesser et al. 2007, Fjeldså et al. 2007).

scholarly journals Sporotrichosis in Mexico

2020 ◽  
Author(s):  
Conchita Toriello ◽  
Carolina Brunner-Mendoza ◽  
Estela Ruiz-Baca ◽  
Esperanza Duarte-Escalante ◽  
Amelia Pérez-Mejía ◽  
...  
Keyword(s):  
Systematic Review ◽  
Geographic Distribution ◽  
Sporothrix Schenckii ◽  
Public Information ◽  
Laboratory Diagnosis ◽  
Sensu Stricto ◽  
Sporothrix Brasiliensis ◽  
Source Of Infection ◽  
Common Clinical Presentation ◽  
Subcutaneous Mycoses

Abstract Sporotrichosis is an endemic mycosis caused by the species of the Sporothrix genus, and it is considered one of the most frequent subcutaneous mycoses in Mexico. This mycosis has become a relevant fungal infection in the last two decades. Today, much is known of its epidemiology and distribution, and its taxonomy has undergone revisions. New clinical species have been identified and classified through molecular tools, and they now include Sporothrix schenckii sensu stricto, Sporothrix brasiliensis, Sporothrix globosa, and Sporothrix luriei. In this article, we present a systematic review of sporotrichosis in Mexico that analyzes its epidemiology, geographic distribution, and diagnosis. The results show that the most common clinical presentation of sporotrichosis in Mexico is the lymphocutaneous form, with a higher incidence in the 0–15 age range, mainly in males, and for which trauma with plants is the most frequent source of infection. In Mexico, the laboratory diagnosis of sporotrichosis is mainly carried out using conventional methods, but in recent years, several researchers have used molecular methods to identify the Sporothrix species. The treatment of choice depends mainly on the clinical form of the disease, the host’s immunological status, and the species of Sporothrix involved. Despite the significance of this mycosis in Mexico, public information about sporotrichosis is scarce, and it is not considered reportable according to Mexico’s epidemiological national system, the “Sistema Nacional de Vigilancia Epidemiológica.” Due to the lack of data in Mexico regarding the epidemiology of this disease, we present a systematic review of sporotrichosis in Mexico, between 1914 and 2019, that analyzes its epidemiology, geographic distribution, and diagnosis.

scholarly journals Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods

2017 ◽  
Vol 61 (10) ◽  
Author(s):  
A. Espinel-Ingroff ◽  
D. P. B. Abreu ◽  
R. Almeida-Paes ◽  
R. S. N. Brilhante ◽  
A. Chakrabarti ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Antifungal Agents ◽  
Collaborative Study ◽  
Sporothrix Schenckii ◽  
International Study ◽  
Sensu Stricto ◽  
Content Type ◽  
Cutoff Values ◽  
Definition Of ◽  
Species Specific

ABSTRACT Clinical and Laboratory Standards Institute (CLSI) conditions for testing the susceptibilities of pathogenic Sporothrix species to antifungal agents are based on a collaborative study that evaluated five clinically relevant isolates of Sporothrix schenckii sensu lato and some antifungal agents. With the advent of molecular identification, there are two basic needs: to confirm the suitability of these testing conditions for all agents and Sporothrix species and to establish species-specific epidemiologic cutoff values (ECVs) or breakpoints (BPs) for the species. We collected available CLSI MICs/minimal effective concentrations (MECs) of amphotericin B, five triazoles, terbinafine, flucytosine, and caspofungin for 301 Sporothrix schenckii sensu stricto, 486 S. brasiliensis, 75 S. globosa, and 13 S. mexicana molecularly identified isolates. Data were obtained in 17 independent laboratories (Australia, Europe, India, South Africa, and South and North America) using conidial inoculum suspensions and 48 to 72 h of incubation at 35°C. Sufficient and suitable data (modal MICs within 2-fold concentrations) allowed the proposal of the following ECVs for S. schenckii and S. brasiliensis, respectively: amphotericin B, 4 and 4 μg/ml; itraconazole, 2 and 2 μg/ml; posaconazole, 2 and 2 μg/ml; and voriconazole, 64 and 32 μg/ml. Ketoconazole and terbinafine ECVs for S. brasiliensis were 2 and 0.12 μg/ml, respectively. Insufficient or unsuitable data precluded the calculation of ketoconazole and terbinafine (or any other antifungal agent) ECVs for S. schenckii, as well as ECVs for S. globosa and S. mexicana. These ECVs could aid the clinician in identifying potentially resistant isolates (non-wild type) less likely to respond to therapy.

Sporothrix schenckii sensu stricto Isolated from Soil in an Armadillo’s Burrow

2014 ◽  
Author(s):  
Anderson Messias Rodrigues ◽  
Eduardo Bagagli ◽  
Zoilo Pires de Camargo ◽  
Sandra de Moraes Gimenes Bosco
Keyword(s):  
Sporothrix Schenckii ◽  
Sensu Stricto

scholarly journals Resolving a century-old case of generic mistaken identity: polyphyly of Chitoniscus sensu lato resolved with the description of the endemic New Caledonia Trolicaphyllium gen. nov. (Phasmatodea, Phylliidae)

ZooKeys ◽  
2021 ◽  
Vol 1055 ◽  
pp. 1-41
Author(s):  
Royce T. Cumming ◽  
Stephane Le Tirant ◽  
Thies H. Büscher
Keyword(s):  
Type Species ◽  
New Genus ◽  
New Caledonia ◽  
Sensu Stricto ◽  
New Combinations ◽  
Mistaken Identity ◽  
Molecular Studies

With every molecular review involving Chitoniscus Stål, 1875 sensu lato samples from Fiji and New Caledonia revealing polyphyly, the morphology from these two distinct clades was extensively reviewed. Morphological results agree with all previously published molecular studies and therefore Trolicaphylliumgen. nov. is erected to accommodate the former Chitoniscus sensu lato species restricted to New Caledonia, leaving the type species Chitoniscus lobiventris (Blanchard, 1853) and all other Fijian species within Chitoniscus sensu stricto. Erection of this new genus for the New Caledonian species warrants the following new combinations: Trolicaphyllium brachysoma (Sharp, 1898), comb. nov., Trolicaphyllium erosus (Redtenbachher, 1906), comb. nov., and Trolicaphyllium sarrameaense (Größer, 2008a), comb. nov. Morphological details of the female, male, freshly hatched nymph, and egg are illustrated and discussed alongside the Chitoniscus sensu stricto in order to differentiate these two clades which have been mistaken as one for decades.

scholarly journals Occurrence of Anisakis and Hysterothylacium nematodes in Atlantic chub mackerels from Libyan coasts

2019 ◽  
Vol 56 (4) ◽  
pp. 347-352 ◽  
Author(s):  
S. Cavallero ◽  
R. A. El Sherif ◽  
A. Pizzarelli ◽  
A.A. El Fituri ◽  
M. El Showhdi ◽  
...  
Keyword(s):  
Risk Mitigation ◽  
Sensu Stricto ◽  
Mitigation Strategies ◽  
Parasitic Nematodes ◽  
Predominant Species ◽  
Scomber Colias ◽  
Zoonotic Risk ◽  
Risk Mitigation Strategies ◽  
Hysterothylacium Aduncum

SummaryThe occurrence of zoonotic parasitic nematodes in Atlantic chub mackerels (Scomber colias syn. Scomber japonicus) from Libyan waters was investigated, using epizootiological estimations and molecular specific characterization of larvae. Nematodes belonging to Anisakis spp., the main etiological agent of anisakiasis in Mediterranean waters, and to Hysterothylacium spp. so far considered not pathogenic to humans, were detected. Prevalence values were generally high in visceral cavities (over 40 % for both parasites) while were low for Anisakis (around 1 %) and null for Hysterothylacium in muscles. Moreover, the level of infections was associated with seasons, a feature potentially useful to plan fishing captures and to elaborate risk mitigation strategies for anisakiasis. Species molecular identification performed on a subsample described the presence of Hysterothylacium aduncum as the predominant species, along with Anisakis pegreffii and the hybrids (A. pegreffii and A. simplex sensu stricto), thus posing a concrete zoonotic risk following the consumption of such fish species as a raw preparation.

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