scholarly journals Cytological Studies in Cluster Bean by the Application of Physical and Chemical Mutagens (Cyamopsis tetragonoloba L.)

2014 ◽  
Vol 21 ◽  
pp. 35-40 ◽  
Author(s):  
L. Mullainathan ◽  
T. Aruldoss ◽  
S. Velu

The miotic and meotic behaviour is considered to be one of the most dependable indices to estimate the potency of mutagen cytological aberrations in plants serve as an excellent monitoring system for the determination of mutagens that may cause a genetic hazard in the present study cluster bean contains 7 bivalents (24 = 14) for the present investigation the plant exposed to dolf concentration of 60, 80, and 100kr gamma rays and 0.4, 0.6 and 0.8 % Mm ( EMS ) the present study reveals various chromosomal aberration includes stickiness precocisus movement ,single and anaphasis bridge laggards and synchronized movement of chromosomes at anaphase

Author(s):  
Devi Suresh ◽  
M. Ananthan ◽  
C. Vanniarajan ◽  
P. Balasubramanian ◽  
T. Sivakumar ◽  
...  

Background: MDU-1 is a high yielding, indeterminate cluster bean variety. In order to develop determinate mutant with high yield, MDU-1 cluster bean variety was irradiated using gamma rays, electron beam and combination of gamma rays and EMS with different doses and the variability induced in M5 generations were studied. Methods: Twenty one best individual plants from M4 generation were selected based on plant height and higher yield and forwarded to M5 generation where they were evaluated in RBD with three replications. Fourteen non-segregating mutant lines with desirable traits were identified in M5 generation and were evaluated for variability along with the parent MDU1. Result: Five best mutant lines namely ACMC-020-04, ACMC-020-11, ACMC-020-08, ACMC-020-10 and ACMC-020-11 were selected from the 14 accessions based on per se performance and variability analysis. The selected mutants needs to be forwarded for stability testing in different environments.


OCL ◽  
2019 ◽  
Vol 26 ◽  
pp. 35 ◽  
Author(s):  
Souhail Channaoui ◽  
Mostapha Labhilili ◽  
Mohamed Mouhib ◽  
Hamid Mazouz ◽  
Mohamed El Fechtali ◽  
...  

Genetic variability is a prerequisite for any plant breeding program, and mutagenesis is a proven way of creating new variation within a crop germplasm. Novel genetic variability in rapeseed was induced by gamma rays, Ethyl Methane Sulphonate (EMS) and combined mutagen treatment, using various doses and concentrations. The objective was to evaluate and compare the obtained M2 mutants for important quantitative traits in two contrasted environments. Data on phenological, morphological and agronomic parameters were recorded. A large variability was observed and mutagenic treatments had a significant effect on all traits studied. Compared to control plants, mutant genotypes derived from seeds treated with low EMS concentrations during moderate time were earlier and characterized by a higher number of pods per plant. For high concentration of EMS during long time and for combinations of physical and chemical mutagens, a significant decrease in plant height and stature was noticed, as compared to control. Besides, plants derived from gamma rays-treated seeds exhibited the highest 1000-seed weight. The novel induced variability may be integrated in rapeseed breeding program as a new germplasm with improved agronomic traits. Particularly, EMS1-7-stable mutant may be exploited to develop efficiently and quickly a new rapeseed cultivar with some desirable traits. The present study highlights once more the possibility to bring novel genetic diversity for rapeseed desirable traits improvement through mutation breeding.


2017 ◽  
Vol 1 ◽  
pp. 19
Author(s):  
R. Elangovan and P. Pavadai

Mutation breeding in crop plants such as bhendi (Abelmoschus esculentus (L). Moench) is a successful approach in change of product having narrow genetic base. In the present study to the determine the effect of physical mutagen such as gamma rays and chemical mutagens such as Ethyl methane sulphonate (EMS) and Diethyl sulphate (DES) were used. The seeds were treating with different doses/concentration of Gamma irradiation (10KR, 20KR, 30KR, 40KR, 50KR and 60KR), EMS and DES (0.1, 0.2, 0.3, 0.4, 0.5, and 0.6%) for six hours were applied to 200 seed sample of each concentration and one respective control on bhendi. plants of four generations viz., M1, M2, M3 and M4. The cytological analysis, for example, chromosomal number was watched and recorded for images. The maximum changes of chromosome were observed in 50 KR of gamma rays and 0.4 % of EMS treatments than the other physical and chemical mutagens. The length and shape of chromosome for varied in treated plants than the untreated plants.


Author(s):  
O.S. Bezuglova ◽  

Rostov Region belongs to the highly protected natural territories characterized by the continuous plowing. There territories are the only reserves with the soils preserved in their natural state. However, these areas often lack detailed information about the soils quality and composition. Surveying soils on these territories is crucial for determination of their basic physical and chemical properties. The resulted compilation of soil maps could lay a foundation for creating the Red Book of Soils and the formation of a section in the soil-geographical database of the Russian Federation. Subsequently, such information can be used as a background data for the main types of soils in the region. It will be also valuable during monitoring and justification of conservation measures.


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