scholarly journals Effect of Explants and Low Cost Medium on Morphogenic Response of Aerial Stem and Rhizome Bud Explants of Turmeric (Curcuma longa L.) for Plant Regeneration

2020 ◽  
Vol 11 ◽  
pp. 15-24
Author(s):  
Hewa Fonsekage Lakma Upendri ◽  
Thayamini H. Seran
Keyword(s):  
Low Cost ◽  
Stem Explants ◽  
Ms Medium ◽  
Shoot Bud ◽  
Rhizome Bud ◽  
Aerial Stem ◽  
The Cost ◽  
Bud Initiation ◽  
Embryogenic Response

This study was done to select suitable explants and low cost medium for plant regeneration of turmeric. Therefore, the different explants were excised from the aerial stems and rhizome buds and surface sterilized. The sterilized explants were cultured on MS medium fortified with 2.0 mg/l BAP. From the survived aerial stem explants, 0.5 cm long vertical half of the aerial stem explants exhibited somatic embryogenic response (69.7%). The highest morphogenic response (74%) of shoot bud initiation was observed from the top slice of the surviving rhizome bud explants. Further, Yara Mila complex fertilizer, which is an ideal granular fertilizer mixture, was used as an alternative to MS medium. Three different concentrations of Yara Mila complex fertilizer (1.0, 3.0, and 5.0 g/l ) supplemented with 2.0 mg/l BAP each were tested with the MS medium fortified with 2.0 mg/l BAP (control treatment) for in vitro establishment from aerial stem explants and top slice of the rhizome bud explants. Both explants were surface sterilized and cultured on MS medium and different concentrations of Yara Mila complex fertilizer fortified with 2.0 mg/l BAP. From the survived explants, aerial stem explants exhibited somatic embryogenic response (69.7%) and shoot bud initiation (74%) on normal MS media. The higher performances were observed in 1.0 g/l concentration of complex fertilizer incorporated medium with 51% embryogenic response from the aerial stem explants and 52.3% shoot bud formation response from the top slice of the rhizome bud. The cost of 1 kg complex fertilizer was Rs. 182. It could be concluded that complex fertilizer is a cost effective alternative medium for MS medium for in vitro propagation reducing the cost of the substituted ingredients by 99.87% in the tissue culture of turmeric.

scholarly journals Propagation of Lily Arumsari using Generic Media through In Vitro Culture

2020 ◽  
Vol 11 (2) ◽  
pp. 140-148
Author(s):  
Ridho Kurniati ◽  
Fauziah Khairatunnisa ◽  
Reni Indrayanti
Keyword(s):  
Plant Growth Regulator ◽  
Low Cost ◽  
Ms Medium ◽  
Single Factor ◽  
Mass Propagation ◽  
Murashige And Skoog Medium ◽  
Number Of Leaves ◽  
Leaves And Roots ◽  
The Cost

Lily was usually propagated using MS (Murashige and Skoog) medium supplemented with plant growth regulator and hormone. The important concerns in mass propagation and seed production of lily for industries are cheap, easy and low cost medium. This study’s objective was to find out of generic media to substitute MS medium and to decrease the cost of mass propagation of lily in vitro. Three substitutions medium were Vitagrow, Growmore, Gibril, and G-60. Bulbs of lily Arumsari varieties were used as materials. Complete Random Design with a single factor was used in the experimental design. The treatments were type of generic in vitro medium (Vitagrow, Growmore, Gibril, and G-60), consisting of three replication and 20 units per repetition and five bulbs per unit of repetition. The parameter observed was the total number of leaves and roots, length of leaves and roots, and bulb growth percentage. Growmore medium showed a better result than others in total number of leaves (50,67), length of leaves (1,6 cm), length of roots (0,312 cm) and percentage of bulb growth (100%). The highest total number of roots was achieved in G-60 medium.

scholarly journals High Frequency Micropropagation of Aloe vera L. Burm. f. as a Low Cost Option Towards Commercialization

2010 ◽  
Vol 20 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Anusree Das ◽  
Priyanka Mukherjee ◽  
Timir Baran Jha
Keyword(s):  
Low Cost ◽  
Aloe Vera ◽  
In Vitro Rooting ◽  
Shoot Bud ◽  
Rooting Medium ◽  
Bud Induction ◽  
Aloe Gel ◽  
The Cost ◽  
First Time

An efficient micropropagation protocol has been developed using shoot apical meristem as explants in a high barbaloin content 'bitter' cultivar of Aloe vera L. The protocol involves induction, multiplication and in vitro rooting of the regenerated shoots and their acclimation under ex vitro conditions. 35.5 µM BAP and 9.8 µM IBA in combination with 81.4 µM adenine sulphate proved optimum for shoot bud induction. Combination of 8.87 µM BAP and 2.46 µM IBA produced highest number of shoot buds (22.0 ± 0.14) and enhanced bud proliferation within one - two weeks after first subculture. For induction of in vitro rooting, Aloe gel as an alternative to conventional rooting medium used for the first time resulted in 100% rooting and highest number of roots per culture (10.90 ± 0.17). The plantlets were successfully hardened. Cent per cent plants survived in the field condition. Chromosomal analysis of the regenerated plantlets established a stable germplasm with 2n = 14 bimodal chromosomes. The cost effectiveness and economic viability of the protocol has also been evaluated. Key words: Aloe vera, bitter cultivar, micropropagation, cost analysis D.O.I. 10.3329/ptcb.v20i1.5962 Plant Tissue Cult. & Biotech. 20(1): 29-35, 2010 (June)

scholarly journals STUDI PENGARUH MEDIA ALTERNATIF UNTUK PERBANYAKAN PISANG BARANGAN (Musa acuminata L.) SECARA IN-VITRO

2021 ◽  
Vol 12 (1) ◽  
pp. 33
Author(s):  
Rosmaina Rosmaina ◽  
Ragil Endika ◽  
Zulfahmi Zulfahmi
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Low Cost ◽  
Ms Medium ◽  
Liquid Fertilizer ◽  
Culture Techniques ◽  
Randomized Design ◽  
Media Component ◽  
The Cost

The main obstacles in the commercialization of seedlings mass propagation through tissue culture techniques is the high cost of the culture media component. therefore, the production of lowcost tissue culture is required. This study aims to develop low cost in-vitro media for the production of the seedlings of Barangan banana. The research was composed following Factorial Completely Randomized Design, the first factor was Terra Novalgro liquid fertilizer with three concentrations, namely 1, 2, and 3 ml L-1, while the second factor was Gandasil with 3 concentrations namely 1, 2, and 3 mg L-1, so obtained nine treatments, each treatment was repeated 10 times, so that there were 90 experimental units. MS media was used as control. The parameter observed was number of shoots, number of leaves and number of roots. The results of this study exhibited that the treatment of 1 ml L-1 liquid fertilizer + 2 mg L-1 foliar fertilizer produced 9.30 shoots/explant and 1.90 leaves/explant, that no significantly different from MS medium (control) which produced 9.0 shoots/explant and 0.3 leaves/explants. Therefore, the using completed liquid fertilizers and foliar fertilizers as medium in vitro propagation of barangan bananas can become an alternative replace MS medium, as well as reduced the cost of culture media as 91% -93% compared to MS media.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals Potential of Launea taraxacifolia (Willd) Amin Ex. C. Jeffrey for in Vitro Regeneration

2011 ◽  
Vol 3 (3) ◽  
pp. 93-96
Author(s):  
Ayobola M.A. SAKPERE ◽  
Ejeoghene R. AYISIRE ◽  
Olufemi I. ABIOYE
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Dichlorophenoxyacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
First Report ◽  
Full Strength

This study investigated the potential of Launea taraxacifolia for in vitro regeneration. Stem and leaf explants were inoculated on full strength Murashige and Skoog (MS) medium supplemented with varying concentrations of 2, 4-dichlorophenoxyacetic acid (2,4-D). Leaf explants responded to all concentrations of 2,4-D used while stem explants responded to only two of the 2, 4-D concentrations suggesting that leaf explants might be a better source of explants. Leaf explants generated shoots on medium supplemented with 0.5 mg/l kinetin and 0.1 mg/l 2, 4-D. This study is the first report on in vitro regeneration of Launea taraxacifolia.

scholarly journals Effect of Different Concentrations of Plant Growth Regulators on in Vitro Propagation of Curcuma roscoeana Wall.

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 460D-460
Author(s):  
Chamchuree Sotthikul ◽  
Pimchai Apavatjrut
Keyword(s):  
Plant Growth ◽  
In Vitro Propagation ◽  
Clonal Propagation ◽  
Stem Explants ◽  
Ms Medium ◽  
Chiang Mai ◽  
Perennial Plant ◽  
The Media ◽  
Low Rate

Curcuma roscoeana Wall. is a tuberous perennial plant with tuberous rhizomes. It is an endangered species. In nature, it has a very low rate of multiplication. Propagation of C. roscoeana in vitro was done by culturing 0.5 × 1.0-mm shoot tips from young buds onto modified Murashige and Skoog (MS)+ 0.25 mg/L kinetin. Stem explants 10.0 mm in size, measured from the base of the plantlets longitudinally cut in half, were used in the experiments. The first experiment was done by varying the concentration of both kinetin and NAA, in MS liquid medium, at 0–8.0 mg/L and 0–0.05 mg/L, respectively. There were no significant differences of kinetin and NAA concentrations on the number of plantlets obtained. The 0.5-mg/L kinetin treatment gave the highest yield in number of new plantlets (3.1 plantlets/cultured explant). In the second experiment, various concentrations of BAP from 0 to 8.0 mg/l were tested. 2.8–3.7 plantlets were formed in the media with 0.05–2.0 mg/L of BAP. The most-suitable concentration of BAP was at 1.0 mg/L, providing 3.7 plantlets/cultured explants. Kinetin or BAP alone could be used in MS medium for rapid clonal propagation of C. roscoeana. The rooted plantlets could be successfully transferred into growing pots. Acknowledgement: The studies were supported in part by The King's Initiative Centre for Fruit and Flower propagation and Development, Ban Rai, Chiang Mai.

scholarly journals In Vitro Propagation of Eriostemon myoporoides and Eriostemon `Stardust'

HortScience ◽  
1994 ◽  
Vol 29 (6) ◽  
pp. 686-688 ◽  
Author(s):  
James R. Ault
Keyword(s):  
Root Length ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Length ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
Axillary Shoot Proliferation

Optimal axillary shoot proliferation was obtained from stem explants of a clone of Eriostemon myoporoides DC. on Murashige and Skoog (MS) basal medium containing 0.1 mg BA/liter, and of Eriostemon `Stardust' on MS medium containing 0.5 mg BA/liter. Overall average number of shoots and shoot lengths for all treatments was greater for E. `Stardust' (22.4 shoots and 12.1-mm shoot length) than for E. myoporoides (4.5 shoots and 8.3-mm shoot length). Maximum percent rooting of E. myoporoides (42%) and E. `Stardust' (95%) was obtained on MS medium supplemented with 1.0 mg K-IBA/liter for E. myoporoides and 0.1 mg NAA/liter for E. `Stardust'. Overall average percent rooting and root lengths were greater for E. `Stardust' (42% rooting and 11.0-mm root length) than for E. myoporoides (27% rooting and 2.3-mm root length). For E. `Stardust', reducing sucrose in the rooting medium from 50 to 25 g·liter-1 significantly decreased overall average percent rooting to 1670 and root length to 6.8 mm. Plantlets of both clones were acclimatized in the greenhouse and transferred successfully to soil, although survival was <7070. Chemical names used: N -(phenylmethyl) -l H -purine-6-amine (BA); potassium-l H -indole-3-butyric acid (K-IBA); l-naphthaleneacetic acid (NAA).

scholarly journals Challenges and Opportunity of in vitro Propagation of Paulownia tomentosa Steud for Commercial Production in Nepal

2016 ◽  
Vol 4 (2) ◽  
pp. 155-160
Author(s):  
Lila Bahadur Magar ◽  
Nisha Shrestha ◽  
Saraswoti Khadka ◽  
Jay Raj Joshi ◽  
Jibaraj Acharya ◽  
...  
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Royalty Rate ◽  
Paulownia Tomentosa ◽  
Shoot Bud ◽  
Plastic Bags

Paulownia tomentosa Steud is a fast growing multipurpose tree. In vitro propagation using nodal explants of Paulownia tomentosa was performed by manipulating amount of cytokinin and auxin in culture media. Shoot bud proliferation from explants were assessed in Murashige and Skoog (MS) medium supplemented with various amounts of hormones such as a) 0.5-2.5 mg/l benzylaminopurine (BAP) and 0.1 mg/l naphthalene acetic acid (NAA), b) 0.5-2.5 mg/l BAP and 0.5-2.5 mg/l kinetin (KN) and c) 0.5- 2.5 mg/l BAP and 0.1 mg/l indole-3-acetic acid (IAA). In the present study, we found that hormone combination of BAP and NAA gave optimum growth results. MS medium enriched with 2.0 mg/l BAP and 0.1 mg/l IAA resulted a similar outcome but took 3-4 weeks with respect to the same medium enriched with 1.0 mg/ml BAP and 0.1 mg/ml NAA, which showed response within 2-3 weeks. Shoot length of 2.5-3.5 cm with 3-4 nodes and 8-12 leaves were obtained on MS medium supplemented with 1.0 mg/l BAP and 0.1 mg/l NAA. The acclimatization of explants was done in a polyhouse at 20±5oC for 2-6 weeks. Rooting has been induced in nonsterile sand. Rooted plants were transferred to plastic bags containing mixture of soil, sand and compost in the ratio of 1:1:1.Besides aforementioned issues, there are several other challenges associated with in vitro propagation of P. tomentosa. The plants were established (90%) on MS medium enriched with BAP and NAA and adapted ex vitro with surviving up to 80%. People received an opportunity with this plant because it grows fast and can generate income in 10 years in comparison with others, but at the same time people also have been facing the challenges for plantation of P. tomentosa as government of Nepal does not formulate necessary national policies, legislations and regulatory frameworks in its favor. Thus, system should be developed to set royalty rate of P. tomentosa recognizing its lifetime value.Int J Appl Sci Biotechnol, Vol 4(2): 155-160

scholarly journals Conservation and Production of Ipecac (Cephaelis ipecacuanha Rich.) Plants from Long Term Shoot Cultures

1970 ◽  
Vol 18 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Rituparna Kundu Chaudhuri ◽  
Timir Baran Jha
Keyword(s):  
Growth Conditions ◽  
Shoot Cultures ◽  
Shoot Bud ◽  
Regenerated Plants ◽  
One Year ◽  
Bud Initiation ◽  
Cephaelis Ipecacuanha ◽  
Important Medicinal Plant

In vitro germplasms of ipecac (Cephaelis ipecacuanha Rich.), an important medicinal plant, maintained through reduced growth conditions for more than 12-years, were used as source material for micropropagation. MS with different combinations of Kn (2 mg/l), BAP (2 mg/l), 2iP (2-3 mg/l), NAA (0.2 mg/l) and adenine (40-100 mg/l) as additive was used to induce fresh multiplication of shoots from the nodal meristems and direct shoot bud initiation on the internodal segments. Complete plant regeneration has been achieved from such long term cultures. Regenerated plants maintained their phenotypic and chromosomal stability. Eighty per cent hardened plants, survived in the field condition, are growing well and 25% of them produced flowers within one year. Long term preservation through reduced growth conditions and successful regeneration of morphologically stable plants with stable chromosome numbers (2n = 22) from such long term cultures of ipecae plants.  Key words: Long term culture, Ipecac, micropropagation, flowering D.O.I. 10.3329/ptcb.v18i2.3646 Plant Tissue Cult. & Biotech. 18(2): 157-164, 2008 (December)

scholarly journals In Vitro Propagation of Pumpkin and Ash Gourd through Nodal Segments

1970 ◽  
Vol 16 ◽  
pp. 67-71 ◽  
Author(s):  
ME Haque ◽  
MAR Sarkar ◽  
MA Mahmud ◽  
D Rezwana ◽  
B Sikdar
Keyword(s):  
Shoot Multiplication ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Bud ◽  
Benincasa Hispida ◽  
Ash Gourd

The present study was undertaken with a view to develop an efficient protocol for in vitro multiple shoot formation and subsequent root induction considering various cultural aspects using nodal segments of Cucurbita maxima and Benincasa hispida. The best hormone for shoot multiplication of pumpkin was BAP (2 mg/l), incase of ash-gourd BAP was 1.5 mg/l. For callus induction BAP+2,4-D was best combination for pumpkin and it was 2.0+0.1 mg/l when in ash gourd BAP+NAA was the best combination. IBA + ½MS medium were used for induction of shoot bud root. In both of pumpkin and ash gourd 1.5 mg/l IBA was found best for induction of roots. Key words: In vitro, MS medium, nodal segments, multiplication, rooting, pumpkin and ash gourd.   DOI:10.3329/jbs.v16i0.3743 J. bio-sci. 16: 67-71, 2008

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