scholarly journals In vitro pre-vascularization strategies for tissue engineered constructs – bioprinting and others

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Andy Wen Loong Liew ◽  
Yilei Zhang
Keyword(s):  
Tissue Engineering ◽  
Cell Sheet ◽  
Nutrient Deficiency ◽  
Fabrication Process ◽  
Vascular Networks ◽  
Cell Sheet Engineering ◽  
Tissue Constructs ◽  
The Future ◽  
And Cartilage

 Tissue-engineered products commercially available today have been limited to thin avascular tissue such as skin and cartilage. The fabrication of thicker, more complex tissue still eludes scientists today. One reason for this is the lack of effective techniques to incorporate functional vascular networks within thick tissue constructs. Vascular networks provide cells throughout the tissue with adequate oxygen and nutrients; cells located within thick un-vascularized tissue implants eventually die due to oxygen and nutrient deficiency. Vascularization has been identified as one of the key components in the field of tissue engineering. In order to fabricate biomimetic tissue which accurately recapitulates our native tissue environment, in vitro pre-vascularization strategies need to be developed. In this review, we describe various in vitro vascularization techniques developed recently which employ different technologies such as bioprinting, microfluidics, micropatterning, wire molding, and cell sheet engineering. We describe the fabrication process and unique characteristics of each technique, as well as provide our perspective on the future of the field.

scholarly journals A Concise Review on the Use of Mesenchymal Stem Cells in Cell Sheet-Based Tissue Engineering with Special Emphasis on Bone Tissue Regeneration

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
A. Cagdas Yorukoglu ◽  
A. Esat Kiter ◽  
Semih Akkaya ◽  
N. Lale Satiroglu-Tufan ◽  
A. Cevik Tufan
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethical Issues ◽  
Cell Sheet ◽  
Bone Tissue Regeneration ◽  
Differentiation Potential ◽  
Cell Sheet Engineering ◽  
Application Techniques

The integration of stem cell technology and cell sheet engineering improved the potential use of cell sheet products in regenerative medicine. This review will discuss the use of mesenchymal stem cells (MSCs) in cell sheet-based tissue engineering. Besides their adhesiveness to plastic surfaces and their extensive differentiation potential in vitro, MSCs are easily accessible, expandable in vitro with acceptable genomic stability, and few ethical issues. With all these advantages, they are extremely well suited for cell sheet-based tissue engineering. This review will focus on the use of MSC sheets in osteogenic tissue engineering. Potential application techniques with or without scaffolds and/or grafts will be discussed. Finally, the importance of osteogenic induction of these MSC sheets in orthopaedic applications will be demonstrated.

scholarly journals Potential of 3-D tissue constructs engineered from bovine chondrocytes/silk fibroin-chitosan for in vitro cartilage tissue engineering

Biomaterials ◽  
2011 ◽  
Vol 32 (25) ◽  
pp. 5773-5781 ◽  
Author(s):  
Nandana Bhardwaj ◽  
Quynhhoa T. Nguyen ◽  
Albert C. Chen ◽  
David L. Kaplan ◽  
Robert L. Sah ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Tissue Constructs

scholarly journals Tissue Engineering of Muscles and Cartilages Using Polyelectrolyte Hydrogels

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hyuck Joon Kwon
Keyword(s):  
Tissue Engineering ◽  
In Vivo Studies ◽  
Current Status ◽  
Polyelectrolyte Gel ◽  
Polyelectrolyte Gels ◽  
Joint Surfaces ◽  
Functional Replacement ◽  
And Cartilage

The prevalent nature of osteoarthritis that causes the erosion of joint surfaces and loss of mobility and muscle dystrophy that weakens the musculoskeletal system and hampers locomotion underlies the importance of developing functional replacement or regeneration of muscle and cartilage tissues. Polyelectrolyte gels have high potential as cellular scaffolds due to characteristic properties similar to biological matrixes. A number of in vitro and in vivo studies demonstrated that polyelectrolyte gels are useful for replacement and regeneration of muscle and cartilage tissues. In addition, it was also found that polyelectrolyte gels have high biocompatibility, durability, and resistance to biodegradation. Moreover, polyelectrolyte gels can overcome their drawbacks of mechanical behavior by introducing double network into the gel. This paper reviews the current status and recent progress of polyelectrolyte gel-based tissue engineering for repairs of muscle and cartilage tissues.

scholarly journals Recent Advances in ROS-Responsive Cell Sheet Techniques for Tissue Engineering

2019 ◽  
Vol 20 (22) ◽  
pp. 5656 ◽  
Author(s):  
Min-Ah Koo ◽  
Mi Hee Lee ◽  
Jong-Chul Park
Keyword(s):  
Tissue Engineering ◽  
Cell Sheet ◽  
Cell Detachment ◽  
Oxygen Species ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
New Approach ◽  
Responsive Systems ◽  
Cell Based Therapy ◽  
Responsive Cell

Cell sheet engineering has evolved rapidly in recent years as a new approach for cell-based therapy. Cell sheet harvest technology is important for producing viable, transplantable cell sheets and applying them to tissue engineering. To date, most cell sheet studies use thermo-responsive systems to detach cell sheets. However, other approaches have been reported. This review provides the progress in cell sheet detachment techniques, particularly reactive oxygen species (ROS)-responsive strategies. Therefore, we present a comprehensive introduction to ROS, their application in regenerative medicine, and considerations on how to use ROS in cell detachment. The review also discusses current limitations and challenges for clarifying the mechanism of the ROS-responsive cell sheet detachment.

Tailoring in vitro biological and mechanical properties of polyvinyl alcohol reinforced with threshold carbon nanotube concentration for improved cellular response

RSC Advances ◽  
2016 ◽  
Vol 6 (46) ◽  
pp. 39982-39992 ◽  
Author(s):  
Tejinder Kaur ◽  
Arunachalam Thirugnanam
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Polyvinyl Alcohol ◽  
Bone Tissue ◽  
Cellular Response ◽  
Natural Bone ◽  
Tissue Constructs ◽  
Living Bone

The development of living bone tissue constructs with structural, mechanical and functional similarities to natural bone are the major challenges in bone tissue engineering.

Reconstruction of functional endometrium-like tissue in vitro and in vivo using cell sheet engineering

2014 ◽  
Vol 446 (1) ◽  
pp. 335-340 ◽  
Author(s):  
Soichi Takagi ◽  
Tatsuya Shimizu ◽  
Goro Kuramoto ◽  
Ken Ishitani ◽  
Hideo Matsui ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Cell Sheet Engineering

Cell Sheet-Based Vascularized Myocardial Tissue Fabrication

2018 ◽  
Vol 59 (3-4) ◽  
pp. 276-285 ◽  
Author(s):  
Hyoe Komae ◽  
Minoru Ono ◽  
Tatsuya Shimizu
Keyword(s):  
Regenerative Medicine ◽  
Cardiac Tissue ◽  
Cell Sheet ◽  
Myocardial Tissue ◽  
Engineering Technology ◽  
Cell Sheet Engineering ◽  
Human Cardiomyocytes ◽  
Whole Heart

Background: The development of regenerative medicine in recent years has been remarkable as tissue engineering technology and stem cell research have advanced. The ultimate goal of regenerative medicine is to fabricate human organs artificially. If fabricated organs can be transplanted medically, it will be the innovative treatment of diseases for which only donor organ transplantation is the definitive therapeutic method at present. Summary: Our group has reported successful fabrication of thick functional myocardial tissue in vivo and in vitro by using cell sheet engineering technology which requires no scaffolds. Thick myocardial tissue can be fabricated by stacking cardiomyocyte sheets on the vascular bed every 24 h, so that a vascular network can be formed within the myocardial graft. We call this procedure a multi-step transplantation procedure. After human-induced pluripotent stem cells were discovered and human cardiomyocytes became available, a thick, macroscopically pulsate human myocardial tissue was successfully constructed by using a multi-step transplantation procedure. Furthermore, our group succeeded in fabricating functional human myocardial tissue which can generate pressure. Here, we present our way of fabricating human myocardial tissue by means of cell sheet engineering technology. Key Messages: Our group succeeded in fabricating thick, functional human myocardium which can generate pulse pressure. However, there are still a few problems to be solved until clinically functional human cardiac tissue or a whole heart can be fabricated. Research on myocardial regeneration progresses at such a pace that we believe the products of this research will save many lives in the near future.

scholarly journals Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

2020 ◽  
Author(s):  
Wen Zhao ◽  
Tong Zou ◽  
Hao Cui ◽  
Yangou Lv ◽  
Dengke Gao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Culture Time ◽  
Marrow Mesenchymal Stem Cells ◽  
3D Printed ◽  
Scaffold Group ◽  
And Cartilage

Abstract Background Cell-based tissue engineering represent a promising management for meniscus repair and regeneration. The present study aimed to investigate whether the injection of parathyroid hormone (PTH) (1–34) could promote the regeneration and chondroprotection of 3D printed scaffold seeded with bone marrow mesenchymal stem cells (BMSCs) in a canine total meniscal meniscectomy model. Methods 3D printed poly(e-caprolactone) scaffold seeded with BMSCs was cultured in vitro, and the effects of in vitro culture time on cell growth and matrix synthesis of the BMSCs-scaffold construct were evaluated by microscopic observation and cartilage matrix content detection at 7, 14, 21, and 28 days. After that, the tissue-engineered meniscus based on BMSCs–scaffold cultured for the appropriate culture time was selected for in vivo implantation. Sixteen dogs were randomly divided into four groups: PTH + BMSCs–scaffold, BMSCs–scaffold, total meniscectomy, and sham operation. The regeneration of the implanted tissue and the degeneration of articular cartilage were assessed by gross, histological, and immunohistochemical analysis at 12 weeks postoperatively. Results In vitro study showed that the glycosaminoglycan (GAG)/DNA ratio and the expression of collagen type II (Col2) were significantly higher on day 21 as compared to the other time points. In vivo study showed that, compared with the BMSCs–scaffold group, the PTH + BMSCs–scaffold group showed better regeneration of the implanted tissue and greater similarity to native meniscus with respect to gross appearance, cells composition, and cartilage extracellular matrix deposition. This group also showed less expression of terminal differentiation markers of BMSC chondrogenesis as well as lower cartilage degeneration with less damage on the knee cartilage surface, higher expression of Col2 and lower expression of degeneration markers. Conclusions Our results demonstrated that PTH (1–34) promotes the regenerative and chondroprotective effects of the BMSCs–3D printed meniscal scaffold in a canine model, and thus their combination could be a promising strategy for meniscus tissue engineering.

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